LOCUS       NM_012508               8833 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus ATPase plasma membrane Ca2+ transporting 2
            (Atp2b2), mRNA.
ACCESSION   NM_012508
VERSION     NM_012508.8
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Varshney,S., Adela,R., Kachhawa,G., Dada,R., Kulshreshtha,V.,
            Kumari,R., Agarwal,R. and Khadgawat,R.
  TITLE     Disrupted placental vitamin D metabolism and calcium signaling in
            gestational diabetes and pre-eclampsia patients
  JOURNAL   Endocrine 80 (1), 191-200 (2023)
   PUBMED   36477942
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   Tian,Q., Feng,M.Y., Gu,H.H., Zhang,Z.H., Qian,S.X. and Zhuang,J.H.
  TITLE     [Estradiol regulates the expression of plasma membrane Ca2+-ATPase
            isoform 2 in inner ear of rats]
  JOURNAL   Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 57 (12), 1484-1490
            (2022)
   PUBMED   36707954
  REMARK    GeneRIF: [Estradiol regulates the expression of plasma membrane
            Ca[2+]-ATPase isoform 2 in inner ear of rats].
REFERENCE   3  (bases 1 to 8833)
  AUTHORS   Radzik,T., Boczek,T., Ferenc,B., Studzian,M., Pulaski,L. and
            Zylinska,L.
  TITLE     Calcium Dyshomeostasis Alters CCL5 Signaling in Differentiated PC12
            Cells
  JOURNAL   Biomed Res Int 2019, 9616248 (2019)
   PUBMED   31032369
  REMARK    GeneRIF: Although modification of PMCAs composition could provide
            some protection against calcium overload, reduction of PMCA2
            appeared to be more detrimental to the cells than deficiency of
            PMCA3. Under pathological conditions, including inflammatory CCL5
            action and long-lasting Ca(2+) dyshomeostasis, insufficient cell
            protection may result in progressive degeneration and death of
            neurons.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8833)
  AUTHORS   Boczek,T., Lisek,M., Ferenc,B. and Zylinska,L.
  TITLE     Cross talk among PMCA, calcineurin and NFAT transcription factors
            in control of calmodulin gene expression in differentiating PC12
            cells
  JOURNAL   Biochim Biophys Acta Gene Regul Mech 1860 (4), 502-515 (2017)
   PUBMED   28153703
  REMARK    GeneRIF: higher occupancy of Calm2 and Calm3 promoters by NFATc2
            and increased expression of these genes in response to NFATc2
            silencing were demonstrated in PMCA2 and PMCA3-reduced lines.
REFERENCE   5  (bases 1 to 8833)
  AUTHORS   de Juan-Sanz,J., Nunez,E., Zafra,F., Berrocal,M., Corbacho,I.,
            Ibanez,I., Arribas-Gonzalez,E., Marcos,D., Lopez-Corcuera,B.,
            Mata,A.M. and Aragon,C.
  TITLE     Presynaptic control of glycine transporter 2 (GlyT2) by physical
            and functional association with plasma membrane Ca2+-ATPase (PMCA)
            and Na+-Ca2+ exchanger (NCX)
  JOURNAL   J Biol Chem 289 (49), 34308-34324 (2014)
   PUBMED   25315779
  REMARK    GeneRIF: GlyT2 activity is pre-synaptically controlled by physical
            and functional association with plasma membrane PMCA2, PMCA3, and
            NCX1.
REFERENCE   6  (bases 1 to 8833)
  AUTHORS   Brandt,P. and Neve,R.L.
  TITLE     Expression of plasma membrane calcium-pumping ATPase mRNAs in
            developing rat brain and adult brain subregions: evidence for
            stage-specific expression
  JOURNAL   J Neurochem 59 (4), 1566-1569 (1992)
   PUBMED   1328526
REFERENCE   7  (bases 1 to 8833)
  AUTHORS   Adamo,H.P. and Penniston,J.T.
  TITLE     New Ca2+ pump isoforms generated by alternative splicing of rPMCA2
            mRNA
  JOURNAL   Biochem J 283 (Pt 2) (Pt 2), 355-359 (1992)
   PUBMED   1315513
REFERENCE   8  (bases 1 to 8833)
  AUTHORS   Ikeda,M., Mikuni,M., Nishikawa,T. and Takahashi,K.
  TITLE     A neurochemical study of a new mutant mouse presenting
            myoclonus-like involuntary movement: a possible model of
            spontaneous serotonergic hyperactivity
  JOURNAL   Brain Res 495 (2), 337-348 (1989)
   PUBMED   2765934
REFERENCE   9  (bases 1 to 8833)
  AUTHORS   Shull,G.E. and Greeb,J.
  TITLE     Molecular cloning of two isoforms of the plasma membrane
            Ca2+-transporting ATPase from rat brain. Structural and functional
            domains exhibit similarity to Na+,K+- and other cation transport
            ATPases
  JOURNAL   J Biol Chem 263 (18), 8646-8657 (1988)
   PUBMED   2837461
REFERENCE   10 (bases 1 to 8833)
  AUTHORS   Matsui,K., Mukoyama,M., Adachi,K. and Ando,K.
  TITLE     [Fundamental study on ataxic mice (wriggle mouse Sagami)]
  JOURNAL   Jikken Dobutsu 36 (2), 185-189 (1987)
   PUBMED   3038581
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000148.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by NM_012508.9.
            
            On Mar 11, 2021 this sequence version replaced NM_012508.7.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-169               JACYVU010000148.1  44537894-44538062   c
            170-215             JACYVU010000148.1  44470618-44470663   c
            216-310             JACYVU010000148.1  44411716-44411810   c
            311-827             JACYVU010000148.1  44330899-44331415   c
            828-1025            JACYVU010000148.1  44298806-44299003   c
            1026-1283           JACYVU010000148.1  44293681-44293938   c
            1284-1409           JACYVU010000148.1  44292686-44292811   c
            1410-1535           JACYVU010000148.1  44287058-44287183   c
            1536-1694           JACYVU010000148.1  44277934-44278092   c
            1695-1909           JACYVU010000148.1  44275841-44276055   c
            1910-2152           JACYVU010000148.1  44270645-44270887   c
            2153-2394           JACYVU010000148.1  44255294-44255535   c
            2395-2629           JACYVU010000148.1  44254392-44254626   c
            2630-2809           JACYVU010000148.1  44246972-44247151   c
            2810-2897           JACYVU010000148.1  44246701-44246788   c
            2898-3004           JACYVU010000148.1  44243750-44243856   c
            3005-3196           JACYVU010000148.1  44243067-44243258   c
            3197-3410           JACYVU010000148.1  44240979-44241192   c
            3411-3622           JACYVU010000148.1  44239526-44239737   c
            3623-3730           JACYVU010000148.1  44239322-44239429   c
            3731-3913           JACYVU010000148.1  44237049-44237231   c
            3914-8833           JACYVU010000148.1  44224604-44229523   c
FEATURES             Location/Qualifiers
     source          1..8833
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..8833
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /note="ATPase plasma membrane Ca2+ transporting 2"
                     /db_xref="GeneID:24215"
                     /db_xref="RGD:2176"
     exon            1..169
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            170..215
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            216..310
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            311..827
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     CDS             629..4225
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /EC_number="7.2.2.10"
                     /note="plasma membrane Ca++-ATPase; plasma membrane
                     calcium pump; plasma membrane calcium ATPase; ATPase, Ca++
                     transporting, plasma membrane 2"
                     /codon_start=1
                     /product="plasma membrane calcium-transporting ATPase 2"
                     /protein_id="NP_036640.1"
                     /db_xref="GeneID:24215"
                     /db_xref="RGD:2176"
                     /translation="MGDMTNSDFYSKNQRNESSHGGEFGCSMEELRSLMELRGTEAVV
                     KIKETYGDTESICRRLKTSPVEGLPGTAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEA
                     LQDVTLIILEIAAIISLGLSFYHPPGESNEGCATAQGGAEDEGEAEAGWIEGAAILLS
                     VICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVVGDIAQIKY
                     GDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMVVTAV
                     GVNSQTGIIFTLLGAGGEEEEKKDKKAKQQDGAAAMEMQPLKSAEGGDADDKKKANMH
                     KKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLTECTPVYV
                     QYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATA
                     ICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINAKTLELLVNAIAINSAYTTKIL
                     PPEKEGALPRQVGNKTECGLLGFVLDLRQDYEPVRSQMPEEKLYKVYTFNSVRKSMST
                     VIKMPDESFRMYSKGASEIVLKKCCKILSGAGEPRVFRPRDRDEMVKKVIEPMACDGL
                     RTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITV
                     RMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKL
                     RVLARSSPTDKHTLVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTD
                     VAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQ
                     DSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNILGHA
                     VYQLTLIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIH
                     GERNVFDGIFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGEL
                     VWGQVIATIPTSRLKFLKEAGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRG
                     LNRIQTQIRVVKAFRSSLYEGLEKPESRTSIHNFMAHPEFRIEDSQPHIPLIDDTDLE
                     EDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSL"
     exon            828..1025
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1283
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1409
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1535
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1694
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1909
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2152
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2394
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2629
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2809
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..2897
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            2898..3004
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3005..3196
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3197..3410
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3411..3622
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3623..3730
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3731..3913
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
     exon            3914..8833
                     /gene="Atp2b2"
                     /gene_synonym="PMCA2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggcggagcg cagtgcagcg gcggcggcgg gagctcgggc gggcgcgggg cgcagaggcg
       61 gcggcggcgg cggggacgca gggccgggcc gggccgggcg cgcagcaggg gcggcggcag
      121 cggcagcggc agcggcggcg gcggcatcat cgccccggag cgggcgcagg gtttgccacc
      181 ctcggagtgt ggactaacag cagccgagga gacagagctg aacttggtca cgcagtccct
      241 agagtcagca agacatgggt tccattgtgc agctcctgac acctctgatg taaccagatg
      301 acgtggaggg gagctgtccc gaccatgtag atgccagccc cagggatgat cagcatgggc
      361 cccactgaat ggagactcct gggtcagcat tcctgagcct gttcggcccc tggacctcac
      421 cccactcctg gggacactcc tcagagctct ccagtgctgt cgccagccca cctcagccat
      481 cgcctcctcc tgggactgga agttggcctc ctggagctaa taccctctgg tgagggtgta
      541 ctgaggacag ccacacacag ccatcaccca caggcccctt ttccagtgct gaccctcagg
      601 cccagcctga gcagaaactt cagcaaacat gggtgatatg accaacagcg acttttactc
      661 caaaaaccaa agaaatgagt cgagccatgg gggcgagttc gggtgcagca tggaagagct
      721 gcgctccctc atggagctgc ggggcactga ggctgtggtc aagatcaagg agacatatgg
      781 ggacactgaa tccatctgcc ggcgcctcaa aacctctcct gttgaaggtt tgccaggcac
      841 cgctccagac ctggaaaaga ggaaacagat tttcgggcaa aacttcatac ctccaaagaa
      901 accaaaaacc ttcctgcagc tggtgtggga agcactacag gacgtgacgc tcatcatcct
      961 ggagatcgcg gccatcatct ccctgggact gtccttctac cacccacccg gagagagcaa
     1021 tgaaggatgt gccacggccc aaggtggggc agaagatgaa ggtgaagcag aagcaggctg
     1081 gatcgagggg gctgccatcc tgctgtcagt catctgtgtg gtcctcgtca cagccttcaa
     1141 tgattggagt aaagagaaac agttccgggg cctgcagagc cgaattgagc aggaacagaa
     1201 gttcaccgta gtccgggctg gccaggtggt ccagatccct gtggccgaga ttgtggttgg
     1261 ggacattgcc cagatcaaat acggtgacct ccttcctgct gatggcctct tcatccaggg
     1321 caatgacctc aagatcgatg aaagctcgct cacaggggag tctgaccagg tgcgcaagtc
     1381 tgtggataag gaccctatgt tgctgtcagg aactcatgtg atggaaggct caggacggat
     1441 ggtggtgact gctgtgggtg tcaactctca aactggcatc atatttaccc tgcttggggc
     1501 tggtggtgaa gaggaagaga agaaagacaa aaaagccaag cagcaggatg gggcagctgc
     1561 tatggaaatg cagcctctga agagtgcgga gggcggtgat gcagatgaca agaagaaagc
     1621 caacatgcac aagaaagaga agtcggtgct tcagggcaag ctcaccaagc tggctgtgca
     1681 gataggcaag gcgggcctgg tgatgtcggc catcacggtg atcatcctgg tgctctactt
     1741 caccgtggac accttcgtgg tcaacaagaa gccgtggctg acggagtgca caccggtcta
     1801 cgtgcagtac ttcgtcaagt tcttcatcat tggtgtgacg gtgctggtgg tcgctgtgcc
     1861 cgagggcctc cctctggctg tcaccatctc actggcctat tccgtgaaga aaatgatgaa
     1921 ggacaacaac ctggtccgtc acctggacgc ctgtgagacc atgggtaacg ccacagccat
     1981 ctgctcagac aagacaggca cgctgaccac caaccgcatg accgtggtgc aggcctatgt
     2041 cggtgatgtc cactacaagg agatccctga tcccagctcc atcaatgcca agacgttgga
     2101 gctgctggtc aacgccattg ccatcaacag cgcctacacc accaagatcc tccccccaga
     2161 aaaagagggc gccctgcccc ggcaggtggg caacaagaca gagtgtggcc tgctgggctt
     2221 cgtgctggac ctgaggcagg attacgagcc ggtacgcagc cagatgccag aggaaaagct
     2281 gtataaggtg tacaccttca actctgtgcg caagtccatg agcaccgtca tcaagatgcc
     2341 tgacgagagc ttccgcatgt acagcaaagg tgcttcggag attgtgctca aaaagtgctg
     2401 caagattctc agtggggcag gggagccccg tgtcttccgg ccccgtgaca gggatgagat
     2461 ggttaagaag gtgatcgagc ccatggcctg tgacgggctc cgtaccatct gtgtggccta
     2521 tcgtgacttc cccagcagcc ctgagcctga ctgggacaat gagaatgaca ttctcaatga
     2581 actcacctgc atctgcgtgg tgggcatcga agacccagtg cgacctgagg tcccagaagc
     2641 catccgcaag tgccagcggg ccggtatcac agtccgcatg gtcaccggtg acaacatcaa
     2701 cacagcccgg gccatcgcca tcaagtgtgg cattatccac cctggagagg acttcctgtg
     2761 cctggaaggc aaagaattca atcggaggat ccgcaacgag aagggggaga ttgagcagga
     2821 gcggattgac aagatctggc caaagctgag ggtgctggct cgatcctcgc ccacggataa
     2881 gcacaccctg gtcaaaggca tcatcgacag cacacacact gagcagcggc aggtggtggc
     2941 tgtgacaggg gatgggacca acgatgggcc tgctctcaag aaggcagatg tgggcttcgc
     3001 gatgggcatc gcaggcacgg atgtggccaa ggaggcctca gacatcatcc tgacagacga
     3061 caacttcagc agcatcgtta aggcagtgat gtggggccgt aatgtctatg acagcatatc
     3121 caaattcctg cagtttcagc tgactgtcaa tgtggtggct gtgatcgtgg ccttcacggg
     3181 tgcctgcatt acacaggact cccctctcaa ggcggtgcag atgctctggg tgaacctcat
     3241 catggacaca tttgcttccc tggccctggc aacagagcca cccaccgaga ctctgctcct
     3301 gaggaaacct tacggccgca acaagccgct catctcgaga accatgatga agaacatcct
     3361 gggccacgcc gtctaccagc taaccctcat cttcaccctg ctcttcgtgg gtgagaagat
     3421 gttccagatt gacagcggaa ggaacgcccc gctgcactcg ccaccctcgg agcactacac
     3481 catcatcttc aacaccttcg tcatgatgca gcttttcaac gagatcaacg cccgtaagat
     3541 ccacggcgag cgtaacgtct tcgacggaat cttccggaac cccatcttct gcaccatcgt
     3601 tctcggcact tttgccatcc agatagtgat tgtgcagttc ggcggcaaac ccttcagctg
     3661 ctccccactc cagctggacc agtggatgtg gtgcatcttc ataggcctgg gagagctcgt
     3721 ctggggccag gtcattgcca ccatccctac cagcaggctc aagtttctga aagaggcagg
     3781 gcggctaaca cagaaggagg agatccccga ggaagagtta aatgaggatg tggaagagat
     3841 agaccacgca gagcgggagc ttcgccgagg ccagatccta tggttccggg gcctgaatcg
     3901 gatccagaca cagatccgcg tcgtgaaggc gttccgtagc tctctctatg aagggttaga
     3961 aaaacccgag tctcgaacct ccatccataa cttcatggct catcctgaat tccggatcga
     4021 agattcccag ccccacatcc ccctcatcga tgacaccgac ctggaagaag atgccgcgct
     4081 caagcagaac tcgagcccgc cgtcctcgct caacaagaac aatagcgcca tcgacagcgg
     4141 gatcaacctg acgaccgaca cgagcaaatc agctacctct tcaagtccag ggagccccat
     4201 ccacagcctg gagacgtcgc tttagctgag gcccctgtcg cctggccatg ctcatggacc
     4261 caccgccacc cgcctccagg cgcacaccct tccaggcacc aactccccga cgcagcaagg
     4321 agcaatgggc ggaacccaca cagcggagag agaacccgtt tccacacaca gaccctttct
     4381 ctggctgcca tgctgtttga actctttttc actccttagg ccatgggcgg ggttgccccc
     4441 ggggtcatct ggagcagaga gtggcgtgga ttgttttttt ttttttttcc aaaacataaa
     4501 aaaataataa cgaattaatt ttaaaaaaaa agagcccttc caggctccag cccagacacg
     4561 gaccagccat gcacccgcca gccactgcgt ccccagcatg aatgtacctg gacactttta
     4621 atcccctccc tcgattggtt cttggagggt tagggagggg agttttttgt ttgttttctt
     4681 aggcgggaac tgcaaacagg aactgcgaac agactgctgt tcattcagtc cactggtcta
     4741 tgaggtttca aatgcttgcc cagcatggtc tcaaatgtct agattaattt aataactttt
     4801 tgaaatcaca gagtaggttt gcaccaatga aacccaagaa ctccacagac actcatgagg
     4861 ttatatccgt ttctttgtat ctatatcaac gtatacttct tgaagactgt atacgtgcgt
     4921 atagataagt agatattata tatatatata catatatata catggatata taaagttcct
     4981 ttgccggcat gttgccttgt ttctgcttaa attgctctat tttaacttat gtcctaaaag
     5041 aagaatgtaa tttgtttaca aacctgtaga taatgtcttt ggctatttgt gtggtttaag
     5101 aacactggtg gccgagatgc ttacaaacag cttggcccaa cagacacatc ccctggattg
     5161 catccttgaa gttttacgat agccatgctc tgattttttg cctgctattt ccgttcaata
     5221 acgttactac tgtgggaagc tcaggcagaa agccaacagc tcccaaggat ccaccctgag
     5281 gagctacgca acatgctcca ccgtagacaa gggagaagag gagagaaggt ggttcccccg
     5341 ggctggcaac atggctgtcc aggtcccatg acgtcagtag tgcacccgga gaaggaaggc
     5401 ttttctctca atgaaactcg cttgagttta cagagagcat ttcagaactg agttgtcttt
     5461 ggcactgtct ttgtgagtca aggtagtgtt cagatatcac agatgctatt gtgttgattt
     5521 tttttttttt ttagttagta gtttagaagt ttctttcctt ataaacagca gcatagatac
     5581 tgctgacata ctggatgagg gttggtacgg cttgctgctt tcggtttttc tttttgaaga
     5641 atagcctttt ttctctgcac tatttagatc cgaatgaacc ttatgatgtg tacattgaga
     5701 tgtattcagt gtgattttta aaccaaattg tcttcctgta gttacaacat atactgtagc
     5761 cttttaacag caagtcctgc tttcccagac agaaagccat tccgattatc ttcccatatc
     5821 acaggtgaga aaaggtggct atcccctccc ccaacaataa cagcactagt aatcccaact
     5881 caataagagc ttgtctgtcg gcctctgact gctaagcact ttgtgggtct cagctttttt
     5941 tcataaaaga acttttgtat ttaatacaaa gtttgctttg agacttttca gcatacaatc
     6001 tttttccata aacttgtaca gtgcaaaaag acattttgaa taccatgacc ggtggtgtcc
     6061 cgtgctttga ggaaaaccaa acactttccg cctcacttgt gaactccatc cttcactctc
     6121 tcccttctca caatcgctcc tcgctcttca gcctggtccc cagccctcct ccaggcccag
     6181 agacctcttc actaaacaag atgacagcca cttgggaaaa gagccatagt ccgctgggag
     6241 ggcccacgtc tggatggctg tgaaaaactt gagtgattgg tttggggttc aaagtctgcc
     6301 cgtcccacct ggccacaatc tctctgtaag gaagaatatc tctgaaggat tctgaatgta
     6361 tccaggagag agagagctgc ctctccagca ggtggaccag ccctagatat actcactgga
     6421 ttccagtaca gactccacca gaggcagccc acagtacccc tccaagggag ggaaggaaac
     6481 cccggggctg ccttcaaaca ggataggttt ttctttgggt tttttgttgt tgtttgattg
     6541 gtttgggggt tttttggttt tgtttttttt gggggggggt tttgtttttt gttttttgtt
     6601 ttttttggtg gggagtcctt tgtcacattt ttcatcctac cctcctccat tcaaccatgc
     6661 atctatgacc tcaacctcat tccatgccac cagggttctt gtgcttacaa accagggtcc
     6721 ttgggctttg gaaccaagcc tcaagggcaa agaggaaggg gttctctgag cagagccagg
     6781 ttctcagaca gggagcagag gaagctagca aggaggcctg acattagcgc tggcttcccg
     6841 gcgtcaggaa tgagggatag cctatgctcc agcccctcgt tctcacactc ttccatacac
     6901 acctacatag gagatggttg gttggtcaga actcactgta gtacacaaag cttgcacttc
     6961 cgcgtcctat atctagcagc atgggatacg tttggcagtt caccccatta gggggtcaat
     7021 aatttatgac cattcatctg tttttatgaa tttttttatc tagacaataa ttgtaaataa
     7081 agaattcacc gtctctgctc atttaatact atgcaatggt tatgttttcc atggctggct
     7141 gttcctgctc acgcaggtat ttctggtgtg tctatgatat ttaaaaacac aaatcaagca
     7201 gaaataggaa atctctactc ttttctagca tgggaccctg cccctccttt cttgtcctct
     7261 gcactctgta agtgcatcag ctccctgccc cctcagaatg ctgagagaca atggggggca
     7321 ctcaacttgc tgctcacagc tatccaggat tgaggagcag cctccatgtc tccacccacc
     7381 cacccctctc ctatcctcaa cccaaccaca gcagagctgt ctggagggag gtgccctatt
     7441 tctgagagtg gatgtggcac tggcagctcc caagccagag cctatcctat cctgggtcct
     7501 ctggttaaaa aagagccact gtgtccccat gcctgttaaa aagctacctt tgcctcctcc
     7561 tgatttttta tatagacctc agtccaattc tagaatttct ctttaacccc cttgtctggc
     7621 accagccaca tgttttaatt attatccccc tcaaacatgt tagtatatag tagggagtaa
     7681 gtccccctcc ctgttcacaa gattccccct cacatctgtt cttctgaaga aatgctaaaa
     7741 tttttgccag accgtctgaa cttctcttac cgcagactct gggccctgtt gctttatgtg
     7801 ccacccctac ccccattgta atcactgtcc atttcagatc ctctatgtgc agaatgtcct
     7861 gtgagaacag acaaaacaac accctgaatt ctgggaaact atcaacttac ctgtcccctt
     7921 agccccagaa ttgtcaggag aggccacaag ccccttctaa caagtcatct gtggttgtcc
     7981 cagagccagt ccccatatgc tccttgactt gatgggaaag gaagacctct cccagagcca
     8041 gtcttggaga gaacattcga tctcatcaga cccacactac agagggatac tttctgtccg
     8101 ccttttctaa actggttttt ggtgatttta tttgggttat tgggagtcat ctcttattcg
     8161 gagttatagc acctttggga tacaagaatg tcttgcctga tgtcttggca cctttgatgt
     8221 agccatagga ggttggcaac tgaaccacac tctgacaagc ctttcatcca actctcttct
     8281 gctctagtat ctaaagcccc acccttggag aaccctagca gtgaggattc ttctagaagc
     8341 accaaggctg cagtcagacc ctaaaagtcc ccatgtgttt tccaaggtcc aaatggagtc
     8401 ctctgattta atatagagat ccgacccacc caaggcatag gaccccaggc cctgggtagc
     8461 tgccatcatc ccctcctccc aaaacaaaag gtttgaggct gcttgggccc ttccaggccc
     8521 atggtctgtt ttgtaatgtc tgtggtgctc tccctccagg gcccttcctc tcgggggtca
     8581 ccacactttg ctaactctcg tgtgcacatc ttttataacg gagtagcgag ggaatggtgc
     8641 cgcctccagc ttctataagc tgcctgggct ctggagggct ctgggacaat tggggctggg
     8701 aagtgactgt gctcttattg tactctcttt atttctctgt atctctggct tgtgctcttt
     8761 gtaactaaca ggatttgtct gccttttcaa cactatactg agcaataaca ataaatgcac
     8821 acgtggaaac gca
//
