LOCUS       NM_022848               3447 bp    mRNA    linear   ROD 23-APR-2024
DEFINITION  Rattus norvegicus ATPase Na+/K+ transporting subunit alpha 4
            (Atp1a4), transcript variant 1, mRNA.
ACCESSION   NM_022848 XM_003754713
VERSION     NM_022848.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Upmanyu,N., Dietze,R., Kirch,U. and Scheiner-Bobis,G.
  TITLE     Ouabain interactions with the alpha4 isoform of the sodium pump
            trigger non-classical steroid hormone signaling and integrin
            expression in spermatogenic cells
  JOURNAL   Biochim Biophys Acta 1863 (11), 2809-2819 (2016)
   PUBMED   27599714
  REMARK    GeneRIF: Ouabain interactions with the alpha4 isoform of the sodium
            pump trigger non-classical steroid hormone signaling and integrin
            expression in spermatocytes.
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Clausen,M.V., Nissen,P. and Poulsen,H.
  TITLE     The alpha4 isoform of the Na,K-ATPase is tuned for changing
            extracellular environments
  JOURNAL   FEBS J 283 (2), 282-293 (2016)
   PUBMED   26476261
  REMARK    GeneRIF: Atp1a4 is tuned for changing extracellular environments
            during sperm capacitation.
REFERENCE   3  (bases 1 to 3447)
  AUTHORS   Laird,J.G., Pan,Y., Modestou,M., Yamaguchi,D.M., Song,H.,
            Sokolov,M. and Baker,S.A.
  TITLE     Identification of a VxP Targeting Signal in the Flagellar Na+ /K+
            -ATPase
  JOURNAL   Traffic 16 (12), 1239-1253 (2015)
   PUBMED   26373354
REFERENCE   4  (bases 1 to 3447)
  AUTHORS   McDermott,J.P., Sanchez,G., Chennathukuzhi,V. and Blanco,G.
  TITLE     Green fluorescence protein driven by the Na,K-ATPase alpha4 isoform
            promoter is expressed only in male germ cells of mouse testis
  JOURNAL   J Assist Reprod Genet 29 (12), 1313-1325 (2012)
   PUBMED   23229519
REFERENCE   5  (bases 1 to 3447)
  AUTHORS   Jimenez,T., Sanchez,G. and Blanco,G.
  TITLE     Activity of the Na,K-ATPase alpha4 isoform is regulated during
            sperm capacitation to support sperm motility
  JOURNAL   J Androl 33 (5), 1047-1057 (2012)
   PUBMED   22441762
  REMARK    GeneRIF: Na,K-ATPase alpha4 activity is up-regulated during sperm
            capacitation through mechanisms that involve both increases in
            molecular activity and levels of alpha4 at the sperm plasma
            membrane.
REFERENCE   6  (bases 1 to 3447)
  AUTHORS   Wagoner,K., Sanchez,G., Nguyen,A.N., Enders,G.C. and Blanco,G.
  TITLE     Different expression and activity of the alpha1 and alpha4 isoforms
            of the Na,K-ATPase during rat male germ cell ontogeny
  JOURNAL   Reproduction 130 (5), 627-641 (2005)
   PUBMED   16264093
REFERENCE   7  (bases 1 to 3447)
  AUTHORS   Blanco,G.
  TITLE     Functional expression of the alpha4 isoform of the Na,K-ATPase in
            both diploid and haploid germ cells of male rats
  JOURNAL   Ann N Y Acad Sci 986, 536-538 (2003)
   PUBMED   12763881
  REMARK    GeneRIF: This isoform is developmentally expressed in haploid and
            diploid spermatozoa.
REFERENCE   8  (bases 1 to 3447)
  AUTHORS   Woo,A.L., James,P.F. and Lingrel,J.B.
  TITLE     Roles of the Na,K-ATPase alpha4 isoform and the Na+/H+ exchanger in
            sperm motility
  JOURNAL   Mol Reprod Dev 62 (3), 348-356 (2002)
   PUBMED   12112599
REFERENCE   9  (bases 1 to 3447)
  AUTHORS   Underhill,D.A., Canfield,V.A., Dahl,J.P., Gros,P. and Levenson,R.
  TITLE     The Na,K-ATPase alpha4 gene (Atp1a4) encodes a ouabain-resistant
            alpha subunit and is tightly linked to the alpha2 gene (Atp1a2) on
            mouse chromosome 1
  JOURNAL   Biochemistry 38 (45), 14746-14751 (1999)
   PUBMED   10555956
REFERENCE   10 (bases 1 to 3447)
  AUTHORS   Shamraj,O.I. and Lingrel,J.B.
  TITLE     A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed
            in testis
  JOURNAL   Proc Natl Acad Sci U S A 91 (26), 12952-12956 (1994)
   PUBMED   7809153
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Sep 4, 2012 this sequence version replaced NM_022848.2.
            
            Transcript Variant: This variant (1) is the longer transcript. Both
            variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U15176.1, SRR26360181.2118574.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                JAXUCZ010000013.1  87251997-87252054   c
            59-230              JAXUCZ010000013.1  87251553-87251724   c
            231-290             JAXUCZ010000013.1  87251309-87251368   c
            291-497             JAXUCZ010000013.1  87248342-87248548   c
            498-611             JAXUCZ010000013.1  87247559-87247672   c
            612-746             JAXUCZ010000013.1  87244593-87244727   c
            747-864             JAXUCZ010000013.1  87244206-87244323   c
            865-1133            JAXUCZ010000013.1  87240158-87240426   c
            1134-1335           JAXUCZ010000013.1  87238659-87238860   c
            1336-1445           JAXUCZ010000013.1  87238155-87238264   c
            1446-1580           JAXUCZ010000013.1  87237824-87237958   c
            1581-1770           JAXUCZ010000013.1  87233783-87233972   c
            1771-1943           JAXUCZ010000013.1  87233480-87233652   c
            1944-2080           JAXUCZ010000013.1  87229054-87229190   c
            2081-2231           JAXUCZ010000013.1  87228524-87228674   c
            2232-2400           JAXUCZ010000013.1  87228073-87228241   c
            2401-2555           JAXUCZ010000013.1  87226547-87226701   c
            2556-2679           JAXUCZ010000013.1  87226300-87226423   c
            2680-2825           JAXUCZ010000013.1  87225504-87225649   c
            2826-2956           JAXUCZ010000013.1  87221327-87221457   c
            2957-3058           JAXUCZ010000013.1  87221124-87221225   c
            3059-3150           JAXUCZ010000013.1  87216689-87216780   c
            3151-3447           JAXUCZ010000013.1  87216139-87216435   c
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q24"
     gene            1..3447
                     /gene="Atp1a4"
                     /note="ATPase Na+/K+ transporting subunit alpha 4"
                     /db_xref="GeneID:29132"
                     /db_xref="RGD:61952"
     exon            1..58
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            59..230
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..3179
                     /gene="Atp1a4"
                     /EC_number="7.2.2.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium/potassium-transporting ATPase subunit alpha-4;
                     sodium pump subunit alpha-4; Na(+)/K(+) ATPase alpha-4
                     subunit; ATPase, Na+/K+ transporting, alpha 4 polypeptide;
                     sodium/potassium-transporting ATPase subunit alpha-4-like"
                     /codon_start=1
                     /product="sodium/potassium-transporting ATPase subunit
                     alpha-4 isoform 1"
                     /protein_id="NP_074039.1"
                     /db_xref="GeneID:29132"
                     /db_xref="RGD:61952"
                     /translation="MEPGKETAATSEQKPRPTLRASNTNRQPKVKRRKKDLEELKKEV
                     VMDDHKLTLDELSAKYSVDLTKGLSVTDAQEILTLNGPNVLTPPPTTPEWIKFCKQLF
                     GGFSLLLWTGSLLCFLAYGIHVSYYQENANKDNLYLGIVLSAVVIITGCFSYYQEAKS
                     SKIMESFKTMVPQQALVIRDGEKMQINVRDVVLGDLVEVKGGDQVPADIRVIAAQGCK
                     VDNSSLTGESEPQSRCPDCTHENPLETRNIIFFSTNCVEGTARGVVIATGDHTVMGRI
                     ASLTSGLTMGKTPIATEIEHFIHIITAVAVFLGVTFFFLSLILGYTWLDAVIFLIGII
                     VANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRM
                     TVAHLWFDKTVYEADTSEEQTTGKTFPKSSDTWFYLARIAGLCNRADFKPHQESLPIT
                     KRTTTGDASESALLKFIEQSYSPVSEMRQKNPKVAEIPFNSTNKYQMSIHLLEDNSEA
                     HVLLMKGAPERILDFCSSFLLNGQEYPMDEEMKTDFQNAYIELGGLGERVLGFCFLNL
                     PSNFSKGFQFNTEELNFPMENLCFAGLISMIDPPRTAVPDAVSKCRSAGIKVIMVTGD
                     HPITAKAIAKSVGIISEANETAEDIAARLNISISQVSNKSIKAIVVHGSELKDMDSGQ
                     LDNILKSYKEIVFARTSPQQKLIIVEGCQRLGAIVAVTGDGVNDSPALKKADIGIAMG
                     ITGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLF
                     IVLSIPLPLGTITILCIDLGTDMVPAISLAYETPESDIMKRLPRNPKTDNLVNDRLIG
                     MAYGQIGMIQALAGFFTYFVILAENGFKPLDLLGIRLYWDDTNLNDLEDTYGQQWTYE
                     QRKVVEFTCQTAFFISIVIVQWADLIICKTRRNSLFKQGMKNKVLIFGLLEETILAAC
                     LSYIPGMDVALRMYPLKINWWFCALPYSVLIFIYDEVRKLIIRRRPGGWLEKETYY"
     exon            231..290
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            291..497
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            498..611
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            612..746
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            747..864
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            865..1133
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1335
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1445
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1580
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            1581..1770
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1943
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2080
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2231
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2400
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            2401..2555
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..2679
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            2680..2825
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..2956
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3058
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            3059..3150
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
     exon            3151..3447
                     /gene="Atp1a4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgacctcggc tcccactgca cttcccacct gccgagcccc tgcatcttca tcattcagta
       61 gagccaacgc acctatatct agagatcaca tgatggagcc tggaaaagag acagcggcca
      121 ctagtgagca gaagccaaga cccaccctta gggcttcaaa tacgaataga cagccgaaag
      181 tgaaaagaag gaagaaagat ttagaggagt tgaagaagga agtggttatg gacgatcaca
      241 agctaacgtt ggatgagctg agtgccaagt actctgtgga cttgaccaag ggtcttagcg
      301 tcacagatgc ccaggaaatt ctgactctaa atggaccgaa tgtactaacc cctcctccaa
      361 ccactccaga atggatcaaa ttctgtaaac aattatttgg tggcttctcc ctcctgctgt
      421 ggactggttc cttgctctgc ttcctggcct atggcataca tgtatcttat taccaagaaa
      481 acgccaacaa agataatctg tacctgggta ttgtactatc tgccgtggtc atcatcactg
      541 gctgcttctc atattaccag gaggcgaaga gctcaaagat catggagtct tttaagacca
      601 tggtgcctca gcaagctctg gtaattcgag atggagagaa aatgcagatc aatgtaagag
      661 atgtggtttt gggagaccta gtagaagtga agggtggaga ccaagtccct gctgatatca
      721 gggttatcgc tgcgcaagga tgtaaggtag acaattcctc cttgactggg gagtcagaac
      781 cacagtctcg ttgccctgat tgcacccatg agaaccctct ggagacacgg aatatcatct
      841 tcttctccac caactgcgta gaaggaacag cccggggtgt tgtgattgct acaggagacc
      901 acacagtaat gggccggatt gcttccctga catcaggatt gacaatgggc aagaccccga
      961 ttgccactga aatcgaacac ttcatccata taatcacagc tgtcgcagtc ttcctcggtg
     1021 tcacgttttt tttcctctca cttatcttgg gctatacctg gctggatgct gtcattttcc
     1081 ttattggcat cattgtggcc aatgtgccag aagggctgct ggccactgtc actgtgtgcc
     1141 tgacgctgac agccaagcgc atggctcgca agaactgcct ggtgaagaac ctggaggcgg
     1201 tggagacgct gggctccacg tccaccatct gctcggacaa gactggcacc ctcacccaga
     1261 accgcatgac ggtggctcat ctgtggtttg ataagactgt gtatgaggct gacaccagcg
     1321 aggagcaaac cacggggaaa acatttccca agtcctctga cacctggttt tacctggctc
     1381 gaattgctgg cctctgcaac cgggccgact ttaagcctca tcaggagtct cttcccataa
     1441 ctaagcggac aacaacaggg gatgcttctg agtcagccct cctcaaattc attgagcagt
     1501 cttacagccc tgtgagtgaa atgagacaga aaaaccccaa ggtggcagag attcctttta
     1561 attctaccaa caagtaccag atgtctattc accttctgga agataactca gaagcccacg
     1621 tgctgctgat gaaaggtgct ccagaaagga tcttggattt ttgctccagt ttcctgctca
     1681 atgggcagga gtaccctatg gacgaagaaa tgaagacaga cttccagaat gcctacatag
     1741 aactgggagg tctgggcgag cgtgtgctgg ggttctgctt cttgaatttg cctagcaatt
     1801 tctctaaggg attccaattt aataccgaag aattaaattt tcccatggag aatctgtgtt
     1861 tcgccggcct catatctatg atcgaccctc ctcgaacggc tgtgcctgat gctgttagca
     1921 agtgccgaag tgcagggatt aaggtaatca tggtaacagg ggatcatccc atcacagcca
     1981 aagctattgc caaaagtgtg ggcatcattt cagaagctaa tgaaacggcg gaggacatcg
     2041 ctgctcggct taatatctcc atcagtcaag tcagtaacaa gtctattaaa gccattgtag
     2101 tgcacggctc agagctgaag gacatggact caggtcagct ggacaatatc ctcaagtcct
     2161 acaaggagat cgtgtttgcg cggacctctc ctcagcagaa gctcatcatc gtggagggct
     2221 gtcagagact gggagccatt gtggcagtga ctggtgacgg ggtgaacgac tcccccgcgc
     2281 tgaagaaggc tgacatcggc attgccatgg gcatcactgg ctctgatgtc tctaagcagg
     2341 cggctgacat gatccttctc gacgacaact ttgcctccat tgtgacgggc gtggaggagg
     2401 gtcgcctcat ctttgacaac ctgaagaagt ccatcgccta taccctgacc agcaacatcc
     2461 ctgagatcac ccccttcctg ctgttcatcg tcttaagtat acccttgcct ctgggtacca
     2521 ttaccattct ctgcattgac ctgggtactg acatggtccc tgctatctcc ttggcttatg
     2581 agacacctga aagtgacatc atgaaaaggc ttccacggaa ccccaaaact gacaatctcg
     2641 tgaacgaccg tcttatcggc atggcctatg ggcagatcgg gatgatacag gcgctggctg
     2701 gatttttcac ctatttcgtg atcctggcgg agaatggttt taagcctctt gatcttctgg
     2761 gtatccgtct atactgggat gatacaaacc taaatgacct agaggacacc tatgggcagc
     2821 agtggaccta tgagcagcgg aaggtggtgg agttcacatg ccaaacagcc ttcttcatca
     2881 gcatcgtgat cgtgcagtgg gctgacctca tcatctgcaa gacgcgcaga aattcactct
     2941 tcaagcaggg catgaaaaac aaagtcctaa tatttggact ccttgaggag acgatcctgg
     3001 cagcttgcct gtcctacatt ccaggcatgg atgtagccct gagaatgtac ccactcaaga
     3061 taaactggtg gttctgcgca cttccctaca gtgttctcat cttcatctat gatgaagttc
     3121 gaaaactcat catccgcaga cggcccggtg gatggctgga gaaggagacg tactactaat
     3181 tgcatcagag aaagatcttc acatgacatc agtctcgaag ccaagactga gtataacaat
     3241 gacatctgag ataaagctga gcagggggcg cgattgtatg ggttgaattt actttggatg
     3301 aggcgagttc tggggctgga gcaagagcat ctggtaggag atggagtgga gaggaggccc
     3361 agcctgctct gcctggggcc cagcgagtag gtgcagtttt gttatatcca gcagccggtg
     3421 gagagtaaac acttggaaca accctca
//
