LOCUS       NM_133517               4019 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Rattus norvegicus ATPase H+/K+ transporting non-gastric alpha2
            subunit (Atp12a), transcript variant 1, mRNA.
ACCESSION   NM_133517
VERSION     NM_133517.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Sarkozy,M., Zvara,A., Gyemant,N., Fekete,V., Kocsis,G.F., Pipis,J.,
            Szucs,G., Csonka,C., Puskas,L.G., Ferdinandy,P. and Csont,T.
  TITLE     Metabolic syndrome influences cardiac gene expression pattern at
            the transcript level in male ZDF rats
  JOURNAL   Cardiovasc Diabetol 12, 16 (2013)
   PUBMED   23320804
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Pestov,N.B., Korneenko,T.V., Shakhparonov,M.I., Shull,G.E. and
            Modyanov,N.N.
  TITLE     Loss of acidification of anterior prostate fluids in Atp12a-null
            mutant mice indicates that nongastric H-K-ATPase functions as
            proton pump in vivo
  JOURNAL   Am J Physiol Cell Physiol 291 (2), C366-C374 (2006)
   PUBMED   16525125
REFERENCE   3  (bases 1 to 4019)
  AUTHORS   Qiu,L.Y., Swarts,H.G.P., Tonk,E.C.M., Willems,P.H.G.M.,
            Koenderink,J.B. and De Pont,J.J.H.H.M.
  TITLE     Conversion of the low affinity ouabain-binding site of non-gastric
            H,K-ATPase into a high affinity binding site by substitution of
            only five amino acids
  JOURNAL   J Biol Chem 281 (19), 13533-13539 (2006)
   PUBMED   16531406
  REMARK    GeneRIF: The substitution of 5 amino acids in the low affinity
            ouabain binding site of non-gastric proton-potassium exchanging
            ATPase in order to increase ouabain binding affinity is reported.
REFERENCE   4  (bases 1 to 4019)
  AUTHORS   Swarts,H.G., Koenderink,J.B., Willems,P.H. and De Pont,J.J.
  TITLE     The non-gastric H,K-ATPase is oligomycin-sensitive and can function
            as an H+,NH4(+)-ATPase
  JOURNAL   J Biol Chem 280 (39), 33115-33122 (2005)
   PUBMED   16046397
REFERENCE   5  (bases 1 to 4019)
  AUTHORS   Li,J., Codina,J., Petroske,E., Werle,M.J., Willingham,M.C. and
            DuBose,T.D. Jr.
  TITLE     The effect of beta-subunit assembly on function and localization of
            the colonic H+,K+-ATPase alpha-subunit
  JOURNAL   Kidney Int 66 (3), 1068-1075 (2004)
   PUBMED   15327400
REFERENCE   6  (bases 1 to 4019)
  AUTHORS   Pestov,N.B., Romanova,L.G., Korneenko,T.V., Egorov,M.V.,
            Kostina,M.B., Sverdlov,V.E., Askari,A., Shakhparonov,M.I. and
            Modyanov,N.N.
  TITLE     Ouabain-sensitive H,K-ATPase: tissue-specific expression of the
            mammalian genes encoding the catalytic alpha subunit
  JOURNAL   FEBS Lett 440 (3), 320-324 (1998)
   PUBMED   9872395
REFERENCE   7  (bases 1 to 4019)
  AUTHORS   Codina,J., Delmas-Mata,J.T. and DuBose,T.D. Jr.
  TITLE     Expression of HKalpha2 protein is increased selectively in renal
            medulla by chronic hypokalemia
  JOURNAL   Am J Physiol 275 (3), F433-F440 (1998)
   PUBMED   9729517
REFERENCE   8  (bases 1 to 4019)
  AUTHORS   Meneton,P., Schultheis,P.J., Greeb,J., Nieman,M.L., Liu,L.H.,
            Clarke,L.L., Duffy,J.J., Doetschman,T., Lorenz,J.N. and Shull,G.E.
  TITLE     Increased sensitivity to K+ deprivation in colonic
            H,K-ATPase-deficient mice
  JOURNAL   J Clin Invest 101 (3), 536-542 (1998)
   PUBMED   9449685
REFERENCE   9  (bases 1 to 4019)
  AUTHORS   Kone,B.C. and Higham,S.C.
  TITLE     A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2
            subunit. Cloning, functional expression, and renal adaptive
            response to chronic hypokalemia
  JOURNAL   J Biol Chem 273 (5), 2543-2552 (1998)
   PUBMED   9446555
REFERENCE   10 (bases 1 to 4019)
  AUTHORS   Crowson,M.S. and Shull,G.E.
  TITLE     Isolation and characterization of a cDNA encoding the putative
            distal colon H+,K(+)-ATPase. Similarity of deduced amino acid
            sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA
            expression in distal colon, kidney, and uterus
  JOURNAL   J Biol Chem 267 (19), 13740-13748 (1992)
   PUBMED   1320029
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Aug 29, 2014 this sequence version replaced NM_133517.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform (a,
            also known as HKalpha2a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U94912.1, M90398.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               JAXUCZ010000015.1  34559209-34559495
            288-440             JAXUCZ010000015.1  34560173-34560325
            441-500             JAXUCZ010000015.1  34562458-34562517
            501-701             JAXUCZ010000015.1  34565029-34565229
            702-815             JAXUCZ010000015.1  34565976-34566089
            816-950             JAXUCZ010000015.1  34566834-34566968
            951-1068            JAXUCZ010000015.1  34567093-34567210
            1069-1337           JAXUCZ010000015.1  34567444-34567712
            1338-1536           JAXUCZ010000015.1  34568652-34568850
            1537-1646           JAXUCZ010000015.1  34568995-34569104
            1647-1781           JAXUCZ010000015.1  34570429-34570563
            1782-1974           JAXUCZ010000015.1  34573520-34573712
            1975-2150           JAXUCZ010000015.1  34575197-34575372
            2151-2287           JAXUCZ010000015.1  34576207-34576343
            2288-2438           JAXUCZ010000015.1  34577941-34578091
            2439-2607           JAXUCZ010000015.1  34579258-34579426
            2608-2762           JAXUCZ010000015.1  34579515-34579669
            2763-2886           JAXUCZ010000015.1  34581092-34581215
            2887-3032           JAXUCZ010000015.1  34581394-34581539
            3033-3166           JAXUCZ010000015.1  34582134-34582267
            3167-3268           JAXUCZ010000015.1  34582443-34582544
            3269-3360           JAXUCZ010000015.1  34582979-34583070
            3361-4019           JAXUCZ010000015.1  34583208-34583866
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..4019
                     /gene="Atp12a"
                     /note="ATPase H+/K+ transporting non-gastric alpha2
                     subunit"
                     /db_xref="GeneID:171028"
                     /db_xref="RGD:620569"
     exon            1..287
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     CDS             279..3389
                     /gene="Atp12a"
                     /EC_number="7.2.2.19"
                     /EC_number="7.2.2.13"
                     /note="isoform a is encoded by transcript variant 1;
                     potassium-transporting ATPase alpha chain 2; proton pump;
                     non-gastric H(+)/K(+) ATPase subunit alpha; ATPase, H+/K+
                     transporting, nongastric, alpha polypeptide;
                     hydrogen/potassium-exchanging ATPase 12A; H-K-ATPase alpha
                     2; HK alpha 2; sodium pump; non-gastric Na(+)/K(+) ATPase
                     subunit alpha"
                     /codon_start=1
                     /product="potassium-transporting ATPase alpha chain 2
                     isoform a"
                     /protein_id="NP_598201.2"
                     /db_xref="GeneID:171028"
                     /db_xref="RGD:620569"
                     /translation="MRRKTEIYSVELNGTKDVKPADQRDDKKFKGAKNKDLEPNKSHE
                     KEELKKELDLDDHRLSNTELEQKYGTNIIQGLSSVRATELLARDGPNTLTPPKQTPEI
                     IKFLKQMVGGFSILLWIGAALCWIAFVIQYVNNSASLDNVYLGAILVLVVILTGIFAY
                     YQEAKSTNIMASFSKMIPQQALVIRDAEKKVISAEQLVVGDVVEIKGGDQIPADIRLV
                     FSQGCKVDNSSLTGESEPQARSTEFTHENPLETKNIGFYSTTCLEGTATGIVINTGDR
                     TIIGRIASLASGVGSEKTPIAIEIEHFVHIVAGVAVSIGIIFFITAVCMKYYVLDAII
                     FLISIIVANVPEGLLATVTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGT
                     LTQNRMTVAHLWFDNQIFVADTSENQTKQAFDQSSGTWASLSKIITLCNRAEFRPGQE
                     SVPIMKRTVVGDASETALLKFSEVILGDVMGIRKRNHKVAEIPFNSTNKFQLSIHETE
                     DPNDKRFLVVMKGAPERILEKCSTIMINGQEQPLDKSSADSFHTAYMELGGLGERVLG
                     FCHLYLPAEQFPQSYIFDVDSVNFPTSNFCFVGLLSMIDPPRSTVPDAVSKCRSAGIK
                     VIMVTGDHPITAKAIAKSVGIISANNETVEDIAKRRNIAVEQVNKREAKAAVVTGMEL
                     KDMTPEQLDELLTNYQEIVFARTSPQQKLIIVEGCQRQDAIVAVTGDGVNDSPALKKA
                     DIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYTLTKNIAE
                     LCPFLIYIVAGLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKKKDRL
                     VNTQLAIYSYLHIGLMQALGGFLVYFTVYAQQGFWPTSLINLRVAWETDDINDLEDSY
                     GQEWTRYQRKYLEWTGSTAFFVAIMIQQIADLIIRKTRRNSIFQQGLFRNKVIWVGIA
                     SQVIVALILSYGLGSVPALSFTMLRVQYWFVAVPHAILIWVYDEMRKLFIRLYPGSWW
                     DKNMYY"
     exon            288..440
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            441..500
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            501..701
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            702..815
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            816..950
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1068
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1337
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1536
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1646
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1781
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            1782..1974
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            1975..2150
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2287
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2438
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            2439..2607
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2762
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            2763..2886
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            2887..3032
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            3033..3166
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3268
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            3269..3360
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
     exon            3361..4019
                     /gene="Atp12a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acagtggctg gtgatcggcc gcggaggtgc ttgctgcgtc cgggttggcg ccttgcttct
       61 gcaacgccaa catcttcgcc ttcaccactc agggacccgg acctcaacac ccgccgcggc
      121 cgcggcagcc gccaccaaaa gttcagactt gattctgtga accacagtcc ggcgcgcgct
      181 aaagcaacgc gcatcttcac gcctatccgg ctcacctcaa caggccagca tccggtgtct
      241 tacatcgttt gccagcgggt tggaccacca gccacagtat gcgccggaaa acagaaattt
      301 attctgtaga gctcaacgga actaaagacg tcaagccagc agaccagagg gatgacaaaa
      361 agttcaaagg agcgaagaac aaggacttgg aacccaacaa gagtcacgag aaagaggagc
      421 tgaagaaaga acttgatctg gatgatcaca gactcagtaa tacagaattg gaacagaaat
      481 atggtaccaa catcatccag ggtctctcca gcgtcagagc tacagagctc ctggctcggg
      541 atgggcccaa cactcttacc cctcccaagc aaactccaga gattatcaag ttcctcaagc
      601 agatggtggg gggcttttcc atcctcctgt ggataggtgc tgccttgtgc tggatcgcat
      661 ttgtgatcca gtacgtcaac aattccgcat ccctagacaa cgtctacttg ggcgccatac
      721 ttgtcctggt tgttatttta acggggattt ttgcttatta ccaagaagcc aaaagcacca
      781 acatcatggc cagctttagt aagatgatcc cccagcaagc tcttgtcatc cgagatgccg
      841 aaaagaaggt catttcagca gagcaactgg tggtggggga cgttgtggag attaaaggag
      901 gggaccagat ccctgcagac atcagactgg tattttccca agggtgcaaa gtagataact
      961 cctctctcac tggggaatct gagccccagg ctcgttcaac tgagtttacc catgaaaacc
     1021 ccttggaaac caagaacata ggcttctatt ccacaacctg cctggaaggt acagcaactg
     1081 gcattgtcat caacacgggt gaccgtacca tcatcgggcg catagcctcc ttggcttcag
     1141 gcgttgggag tgagaaaaca cccattgcca ttgagatcga gcattttgtc catattgtgg
     1201 caggagtggc tgtgtccatt ggcatcattt tcttcatcac cgccgtgtgc atgaagtact
     1261 atgtcctcga cgccatcatc ttcctcatta gcatcattgt ggctaacgtg ccagagggcc
     1321 tcctggctac tgtcactgtt accctgtcac tgacggcaaa acggatggcc aagaagaact
     1381 gcctggtaaa gaatctggag gcagtggaga ctcttggctc cacctccatc atctgctcag
     1441 acaagacggg gaccctgacc cagaacagga tgactgtggc tcacttgtgg tttgacaatc
     1501 agatctttgt ggctgatact agtgaaaacc aaacaaaaca agcctttgac caaagctctg
     1561 gaacctgggc ttccttgtcc aagataataa cactgtgtaa ccgagcagag ttcaggccag
     1621 gccaggagag tgtccccatc atgaagagaa ctgtggttgg agacgcctca gaaactgctc
     1681 tgctaaagtt ctcagaggtc attttgggtg acgtgatggg aattaggaaa agaaaccaca
     1741 aagtggctga aattcctttt aattctacca acaaatttca gctctccata catgagacag
     1801 aggaccctaa tgacaaacgc ttcctcgtgg tgatgaaagg agcccctgag aggatcttag
     1861 agaagtgcag caccatcatg atcaatggcc aggagcagcc actggacaag agctctgctg
     1921 atagcttcca cacagcctac atggagctgg gcggtctggg cgagcgtgtg ttgggtttct
     1981 gtcacctcta cttgccagca gagcagttcc cccaaagtta catctttgat gtcgactccg
     2041 taaactttcc cacttccaac ttctgctttg tggggcttct gtccatgatc gaccctcctc
     2101 ggtctactgt gccagacgca gtctccaaat gtcggagtgc cgggatcaag gttatcatgg
     2161 tcacaggtga tcaccctatc accgccaaag ctattgccaa gagtgtagga atcatatcgg
     2221 ccaacaacga gacagtggaa gacatcgcga aacgccgcaa tatcgctgtg gagcaggtca
     2281 acaaaagaga agctaaagcc gctgtggtga ctggcatgga gctgaaggac atgaccccag
     2341 aacaactgga tgagcttttg accaactacc aagaaatcgt atttgcccgg acatcacccc
     2401 agcagaagtt gatcattgtg gagggctgtc agaggcagga cgccatcgtt gccgtgacgg
     2461 gtgatggagt gaacgactct ccagcactga agaaggcaga catcgggatt gctatgggga
     2521 tagcaggttc tgacgctgct aagaacgcag ctgacatggt cttactagac gacaacttcg
     2581 cgtctatagt cacaggggtg gaggaaggtc gcctgatctt cgacaacctg aagaaaacca
     2641 ttgcgtacac cctgacaaag aacatcgccg agctctgccc ttttttgatc tacatcgttg
     2701 ctgggctccc cctgcctatt ggaaccatca ctatcctatt catcgacctg ggcacagaca
     2761 ttatcccctc cattgccttg gcctatgaga aagcagaaag tgacattatg aataggaaac
     2821 ctcgccacaa gaagaaagac agactggtga acacgcagct ggccatctac tcttaccttc
     2881 acattggtct catgcaagct ctgggaggtt tcctcgtcta tttcactgtc tatgcacagc
     2941 agggcttttg gcccacttct ctcatcaacc tgagggtagc atgggaaaca gatgacataa
     3001 atgacttgga agacagctac gggcaggaat ggacaaggta tcagagaaaa tacctggaat
     3061 ggacaggctc aacggctttc tttgttgcca tcatgatcca acaaattgcg gatctgatca
     3121 tcaggaaaac ccggaggaat tccatctttc agcaggggct cttcagaaat aaagtcatct
     3181 gggtggggat cgcctcacag gtcatcgttg cactgatcct ctcctatggt ctcgggagtg
     3241 tcccagctct gagctttacc atgctcaggg ttcagtactg gtttgtggct gtgccacatg
     3301 ccattttgat ttgggtgtat gacgagatgc gaaagttgtt catcaggctc taccctggaa
     3361 gctggtggga taagaacatg tattattgag accatgtcac tcacaggtct cccagtggcc
     3421 tatcagaggt gttcttcttc aagtttggct acaccttcgg agatctctgc acaatgtgaa
     3481 tgctaccaaa ccccacatga gagagacttt tggacagaaa actgtatgaa gcatacttag
     3541 gttttgtatt ttaaagctga gatttaactg tttatataga atgttcatct ctatgtctgc
     3601 ctcttaattt taaatgatgt gaatttcaga tatcagcata gggaaggata tatttatgtg
     3661 tatgtaaagg tcactgtgtt aaatagcctt gtatatccaa ggcatgtgct ggggtctgcc
     3721 ctaagctaga ctatgattcc tcaggcctta ctttcttact ctcctagagg cccaacagac
     3781 cccctaaatc caccaagcaa actttctgtt tgctggagtt taacctcagc aaagagtacc
     3841 agtgacttaa gcagtaatat ttatttctag taaaacattg tcaagctctc gccagcaatc
     3901 attttgtgtc agtcatgtgt tgtgttgtaa ctgttagatg tattcatgtg tgacatgtgt
     3961 gactagattg gcaggtccag tgaggcctcg tttttaagta aaagatgttt tctaaagcc
//
