LOCUS       NP_598201               1036 aa            linear   ROD 27-APR-2025
DEFINITION  potassium-transporting ATPase alpha chain 2 isoform a [Rattus
            norvegicus].
ACCESSION   NP_598201
VERSION     NP_598201.2
DBSOURCE    REFSEQ: accession NM_133517.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 1036)
  AUTHORS   Sarkozy,M., Zvara,A., Gyemant,N., Fekete,V., Kocsis,G.F., Pipis,J.,
            Szucs,G., Csonka,C., Puskas,L.G., Ferdinandy,P. and Csont,T.
  TITLE     Metabolic syndrome influences cardiac gene expression pattern at
            the transcript level in male ZDF rats
  JOURNAL   Cardiovasc Diabetol 12, 16 (2013)
   PUBMED   23320804
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1036)
  AUTHORS   Pestov,N.B., Korneenko,T.V., Shakhparonov,M.I., Shull,G.E. and
            Modyanov,N.N.
  TITLE     Loss of acidification of anterior prostate fluids in Atp12a-null
            mutant mice indicates that nongastric H-K-ATPase functions as
            proton pump in vivo
  JOURNAL   Am J Physiol Cell Physiol 291 (2), C366-C374 (2006)
   PUBMED   16525125
REFERENCE   3  (residues 1 to 1036)
  AUTHORS   Qiu,L.Y., Swarts,H.G.P., Tonk,E.C.M., Willems,P.H.G.M.,
            Koenderink,J.B. and De Pont,J.J.H.H.M.
  TITLE     Conversion of the low affinity ouabain-binding site of non-gastric
            H,K-ATPase into a high affinity binding site by substitution of
            only five amino acids
  JOURNAL   J Biol Chem 281 (19), 13533-13539 (2006)
   PUBMED   16531406
  REMARK    GeneRIF: The substitution of 5 amino acids in the low affinity
            ouabain binding site of non-gastric proton-potassium exchanging
            ATPase in order to increase ouabain binding affinity is reported.
REFERENCE   4  (residues 1 to 1036)
  AUTHORS   Swarts,H.G., Koenderink,J.B., Willems,P.H. and De Pont,J.J.
  TITLE     The non-gastric H,K-ATPase is oligomycin-sensitive and can function
            as an H+,NH4(+)-ATPase
  JOURNAL   J Biol Chem 280 (39), 33115-33122 (2005)
   PUBMED   16046397
REFERENCE   5  (residues 1 to 1036)
  AUTHORS   Li,J., Codina,J., Petroske,E., Werle,M.J., Willingham,M.C. and
            DuBose,T.D. Jr.
  TITLE     The effect of beta-subunit assembly on function and localization of
            the colonic H+,K+-ATPase alpha-subunit
  JOURNAL   Kidney Int 66 (3), 1068-1075 (2004)
   PUBMED   15327400
REFERENCE   6  (residues 1 to 1036)
  AUTHORS   Pestov,N.B., Romanova,L.G., Korneenko,T.V., Egorov,M.V.,
            Kostina,M.B., Sverdlov,V.E., Askari,A., Shakhparonov,M.I. and
            Modyanov,N.N.
  TITLE     Ouabain-sensitive H,K-ATPase: tissue-specific expression of the
            mammalian genes encoding the catalytic alpha subunit
  JOURNAL   FEBS Lett 440 (3), 320-324 (1998)
   PUBMED   9872395
REFERENCE   7  (residues 1 to 1036)
  AUTHORS   Codina,J., Delmas-Mata,J.T. and DuBose,T.D. Jr.
  TITLE     Expression of HKalpha2 protein is increased selectively in renal
            medulla by chronic hypokalemia
  JOURNAL   Am J Physiol 275 (3), F433-F440 (1998)
   PUBMED   9729517
REFERENCE   8  (residues 1 to 1036)
  AUTHORS   Meneton,P., Schultheis,P.J., Greeb,J., Nieman,M.L., Liu,L.H.,
            Clarke,L.L., Duffy,J.J., Doetschman,T., Lorenz,J.N. and Shull,G.E.
  TITLE     Increased sensitivity to K+ deprivation in colonic
            H,K-ATPase-deficient mice
  JOURNAL   J Clin Invest 101 (3), 536-542 (1998)
   PUBMED   9449685
REFERENCE   9  (residues 1 to 1036)
  AUTHORS   Kone,B.C. and Higham,S.C.
  TITLE     A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2
            subunit. Cloning, functional expression, and renal adaptive
            response to chronic hypokalemia
  JOURNAL   J Biol Chem 273 (5), 2543-2552 (1998)
   PUBMED   9446555
REFERENCE   10 (residues 1 to 1036)
  AUTHORS   Crowson,M.S. and Shull,G.E.
  TITLE     Isolation and characterization of a cDNA encoding the putative
            distal colon H+,K(+)-ATPase. Similarity of deduced amino acid
            sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA
            expression in distal colon, kidney, and uterus
  JOURNAL   J Biol Chem 267 (19), 13740-13748 (1992)
   PUBMED   1320029
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Aug 29, 2014 this sequence version replaced NP_598201.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform (a,
            also known as HKalpha2a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U94912.1, M90398.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     Protein         1..1036
                     /product="potassium-transporting ATPase alpha chain 2
                     isoform a"
                     /EC_number="7.2.2.19"
                     /EC_number="7.2.2.13"
                     /note="potassium-transporting ATPase alpha chain 2; proton
                     pump; non-gastric H(+)/K(+) ATPase subunit alpha; ATPase,
                     H+/K+ transporting, nongastric, alpha polypeptide;
                     hydrogen/potassium-exchanging ATPase 12A; H-K-ATPase alpha
                     2; HK alpha 2; sodium pump; non-gastric Na(+)/K(+) ATPase
                     subunit alpha"
                     /calculated_mol_wt=114787
     Region          1..50
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Region          40..1036
                     /region_name="ATPase-IIC_X-K"
                     /note="sodium or proton efflux -- potassium uptake
                     antiporter, P-type ATPase, alpha subunit; TIGR01106"
                     /db_xref="CDD:273445"
     Site            100..120
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            144..164
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            301..320
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            333..350
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            785..804
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            815..835
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            856..878
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            931..950
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            955
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by PKA. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            965..983
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     Site            999..1019
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (P54708.1)"
     CDS             1..1036
                     /gene="Atp12a"
                     /coded_by="NM_133517.2:279..3389"
                     /note="isoform a is encoded by transcript variant 1"
                     /db_xref="GeneID:171028"
                     /db_xref="RGD:620569"
ORIGIN      
        1 mrrkteiysv elngtkdvkp adqrddkkfk gaknkdlepn kshekeelkk eldlddhrls
       61 nteleqkygt niiqglssvr atellardgp ntltppkqtp eiikflkqmv ggfsillwig
      121 aalcwiafvi qyvnnsasld nvylgailvl vviltgifay yqeakstnim asfskmipqq
      181 alvirdaekk visaeqlvvg dvveikggdq ipadirlvfs qgckvdnssl tgesepqars
      241 tefthenple tknigfystt clegtatgiv intgdrtiig riaslasgvg sektpiaiei
      301 ehfvhivagv avsigiiffi tavcmkyyvl daiiflisii vanvpeglla tvtvtlslta
      361 krmakknclv knleavetlg stsiicsdkt gtltqnrmtv ahlwfdnqif vadtsenqtk
      421 qafdqssgtw aslskiitlc nraefrpgqe svpimkrtvv gdasetallk fsevilgdvm
      481 girkrnhkva eipfnstnkf qlsihetedp ndkrflvvmk gaperilekc stimingqeq
      541 pldkssadsf htaymelggl gervlgfchl ylpaeqfpqs yifdvdsvnf ptsnfcfvgl
      601 lsmidpprst vpdavskcrs agikvimvtg dhpitakaia ksvgiisann etvediakrr
      661 niaveqvnkr eakaavvtgm elkdmtpeql delltnyqei vfartspqqk liivegcqrq
      721 daivavtgdg vndspalkka digiamgiag sdaaknaadm vllddnfasi vtgveegrli
      781 fdnlkktiay tltkniaelc pfliyivagl plpigtitil fidlgtdiip sialayekae
      841 sdimnrkprh kkkdrlvntq laiysylhig lmqalggflv yftvyaqqgf wptslinlrv
      901 awetddindl edsygqewtr yqrkylewtg staffvaimi qqiadliirk trrnsifqqg
      961 lfrnkviwvg iasqvivali lsyglgsvpa lsftmlrvqy wfvavphail iwvydemrkl
     1021 firlypgsww dknmyy
//
