LOCUS       NM_134394               1451 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Rattus norvegicus autophagy related 3 (Atg3), mRNA.
ACCESSION   NM_134394
VERSION     NM_134394.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Zhang,X.X., Lang,Y.F., Li,X., Li,Z., Xu,Y.Q. and Chu,H.Q.
  TITLE     The protective effect of puerarin-loaded mesoporous silicon
            nanoparticles on alcoholic hepatitis through mTOR-mediated
            autophagy pathway
  JOURNAL   Biomed Microdevices 24 (4), 37 (2022)
   PUBMED   36308627
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Frudd,K., Burgoyne,T. and Burgoyne,J.R.
  TITLE     Oxidation of Atg3 and Atg7 mediates inhibition of autophagy
  JOURNAL   Nat Commun 9 (1), 95 (2018)
   PUBMED   29311554
  REMARK    GeneRIF: A thiol-dependent process is being reported that may
            account for impaired autophagy during aging. This is through direct
            oxidation of key autophagy-related (Atg) proteins Atg3 and Atg7.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1451)
  AUTHORS   Liang,B., Xiao,T., Long,J., Liu,M., Li,Z., Liu,S. and Yang,J.
  TITLE     Hydrogen sulfide alleviates myocardial fibrosis in mice with
            alcoholic cardiomyopathy by downregulating autophagy
  JOURNAL   Int J Mol Med 40 (6), 1781-1791 (2017)
   PUBMED   29039471
REFERENCE   4  (bases 1 to 1451)
  AUTHORS   Yoo,B.H., Zagryazhskaya,A., Li,Y., Koomson,A., Khan,I.A.,
            Sasazuki,T., Shirasawa,S. and Rosen,K.V.
  TITLE     Upregulation of ATG3 contributes to autophagy induced by the
            detachment of intestinal epithelial cells from the extracellular
            matrix, but promotes autophagy-independent apoptosis of the
            attached cells
  JOURNAL   Autophagy 11 (8), 1230-1246 (2015)
   PUBMED   26061804
  REMARK    GeneRIF: ATG3 contributes to autophagy induced by the detachment of
            intestinal epithelial cells from the extracellular matrix, but
            promotes autophagy-independent apoptosis of the attached cells
REFERENCE   5  (bases 1 to 1451)
  AUTHORS   Nath,S., Dancourt,J., Shteyn,V., Puente,G., Fong,W.M., Nag,S.,
            Bewersdorf,J., Yamamoto,A., Antonny,B. and Melia,T.J.
  TITLE     Lipidation of the LC3/GABARAP family of autophagy proteins relies
            on a membrane-curvature-sensing domain in Atg3
  JOURNAL   Nat Cell Biol 16 (5), 415-424 (2014)
   PUBMED   24747438
  REMARK    Erratum:[Nat Cell Biol. 2014 Aug;16(8):821]
REFERENCE   6  (bases 1 to 1451)
  AUTHORS   Radoshevich,L., Murrow,L., Chen,N., Fernandez,E., Roy,S., Fung,C.
            and Debnath,J.
  TITLE     ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and
            cell death
  JOURNAL   Cell 142 (4), 590-600 (2010)
   PUBMED   20723759
REFERENCE   7  (bases 1 to 1451)
  AUTHORS   Yang,Z. and Klionsky,D.J.
  TITLE     Mammalian autophagy: core molecular machinery and signaling
            regulation
  JOURNAL   Curr Opin Cell Biol 22 (2), 124-131 (2010)
   PUBMED   20034776
  REMARK    Review article
REFERENCE   8  (bases 1 to 1451)
  AUTHORS   Wu,B.X., Darden,A.G., Laser,M., Li,Y., Crosson,C.E., Hazard,E.S.
            3rd and Ma,J.X.
  TITLE     The rat Apg3p/Aut1p homolog is upregulated by ischemic
            preconditioning in the retina
  JOURNAL   Mol Vis 12, 1292-1302 (2006)
   PUBMED   17110912
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1451)
  AUTHORS   Tanida,I., Ueno,T. and Kominami,E.
  TITLE     LC3 conjugation system in mammalian autophagy
  JOURNAL   Int J Biochem Cell Biol 36 (12), 2503-2518 (2004)
   PUBMED   15325588
  REMARK    Review article
REFERENCE   10 (bases 1 to 1451)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Komatsu,M., Ueno,T. and Kominami,E.
  TITLE     Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple
            substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the
            conjugation of hApg12p to hApg5p
  JOURNAL   J Biol Chem 277 (16), 13739-13744 (2002)
   PUBMED   11825910
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000011.1.
            
            On Mar 29, 2021 this sequence version replaced NM_134394.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078743.1, AF175224.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-391               JAXUCZ010000011.1  69158890-69159280   c
            392-433             JAXUCZ010000011.1  69155750-69155791   c
            434-483             JAXUCZ010000011.1  69151616-69151665   c
            484-554             JAXUCZ010000011.1  69148875-69148945   c
            555-662             JAXUCZ010000011.1  69147274-69147381   c
            663-712             JAXUCZ010000011.1  69143429-69143478   c
            713-794             JAXUCZ010000011.1  69140179-69140260   c
            795-985             JAXUCZ010000011.1  69135842-69136032   c
            986-1113            JAXUCZ010000011.1  69135014-69135141   c
            1114-1182           JAXUCZ010000011.1  69132605-69132673   c
            1183-1451           JAXUCZ010000011.1  69130948-69131216   c
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q21"
     gene            1..1451
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /note="autophagy related 3"
                     /db_xref="GeneID:171415"
                     /db_xref="RGD:708464"
     exon            1..391
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     CDS             320..1264
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /note="autophagy-related protein 3;
                     preconditioning-inducible gene 1 protein; ATG3 autophagy
                     related 3 homolog; APG3 autophagy 3-like"
                     /codon_start=1
                     /product="ubiquitin-like-conjugating enzyme ATG3"
                     /protein_id="NP_599221.2"
                     /db_xref="GeneID:171415"
                     /db_xref="RGD:708464"
                     /translation="MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGD
                     HLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDD
                     GDGGWVDTYHNTGITGITEAVKEITLESKDSIKLQDCSVLCDEEEEEEEGEAADMEEY
                     EESGLLETDEATLDTRRIVEACKAKADAGGEDAILQTRTYDLYITYDKYYQTPRLWLF
                     GYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPPMCSVHPCRHAEVMKKIIET
                     VAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM"
     exon            392..433
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            434..483
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            484..554
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            555..662
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            663..712
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            713..794
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            795..985
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1113
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1182
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1451
                     /gene="Atg3"
                     /gene_synonym="Apg3l; PIG-1; Pig1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcggcgggg ctgttgggag agtgtgagaa gaggaagggg aagccggaag gggttcaagt
       61 ggagcttggg ggacgaaaga cagcttcgag aaggccgggg gctgcgcgcg cacccgtctc
      121 tcgctcagcc ggctgggcct ctgctgtcgc ggccaggtga cagctgggcc gggcctccgc
      181 gtgccgccgc ccctccctcc cggccgggcg ggtgccgctg cgggattcga tgccagtcgg
      241 gcacgtccgg ggcagttttt gactccctgg cccggattgc gacagtctct ccgtgcccca
      301 tcgctgctcc ggtcccagga tgcagaatgt gatcaacacg gtgaagggaa aggctctgga
      361 agtggccgag tacctgaccc cggtcctcaa ggaatcaaaa tttaaggaaa caggtgtaat
      421 caccccagaa gagtttgtgg cagctggaga tcacttagtc caccactgtc caacatggca
      481 atgggctaca ggggaagaat tgaaagtgaa ggcatatcta ccaacaggga aacagttttt
      541 ggtaaccaaa aatgttccat gctacaagcg gtgtaaacag atggagtatt cggatgaatt
      601 ggaggctatc attgaagaag atgatggtga tgggggctgg gtagacacgt accataacac
      661 aggtattaca ggaattactg aagcagttaa ggagattaca ctggaaagca aggacagtat
      721 aaaactccaa gactgctcag tgctgtgcga tgaagaggag gaggaagagg aaggggaagc
      781 tgcagacatg gaagaatatg aagagagtgg attgttggaa acagatgagg ctaccctaga
      841 caccagaaga atagtggaag cttgtaaagc taaagctgat gctgggggtg aagacgccat
      901 tctgcaaaca agaacctatg acctgtacat cacttacgac aagtactacc agacgccacg
      961 gctctggctg tttggctatg atgagcaacg gcagccttta acagttgagc acatgtatga
     1021 agacatcagt caagatcatg tgaagaaaac agtgaccatt gaaaaccatc ctcacctccc
     1081 accacctcct atgtgttcag ttcacccctg caggcatgct gaagtgatga agaaaattat
     1141 tgagaccgtt gcagaaggcg ggggagagct tggtgttcat atgtatcttt taattttttt
     1201 gaaatttgtt caagctgtca ttccaacaat agaatatgac tacacaagac acttcacaat
     1261 gtagtggaga ggatatagag tctatcctaa ttactgcttc tgatttttaa agaattaata
     1321 catagatgtg accattgacc atatttacca ataagaataa tttctctaat aatgggactg
     1381 atatgtttat gcattaaata aaaactttct gctaccagcc ttatttgttt aataaaatgt
     1441 gtgtgaagaa a
//
