LOCUS       NM_001105955            9727 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus assembly factor for spindle microtubules (Aspm),
            mRNA.
ACCESSION   NM_001105955 NM_001198563 XM_001067212 XM_213891
VERSION     NM_001105955.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Vange,P., Bruland,T., Beisvag,V., Erlandsen,S.E., Flatberg,A.,
            Doseth,B., Sandvik,A.K. and Bakke,I.
  TITLE     Genome-wide analysis of the oxyntic proliferative isthmus zone
            reveals ASPM as a possible gastric stem/progenitor cell marker
            over-expressed in cancer
  JOURNAL   J Pathol 237 (4), 447-459 (2015)
   PUBMED   26178168
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Fujimori,A., Itoh,K., Goto,S., Hirakawa,H., Wang,B., Kokubo,T.,
            Kito,S., Tsukamoto,S. and Fushiki,S.
  TITLE     Disruption of Aspm causes microcephaly with abnormal neuronal
            differentiation
  JOURNAL   Brain Dev 36 (8), 661-669 (2014)
   PUBMED   24220505
REFERENCE   3  (bases 1 to 9727)
  AUTHORS   Alsiary,R., Bruning-Richardson,A., Bond,J., Morrison,E.E.,
            Wilkinson,N. and Bell,S.M.
  TITLE     Deregulation of microcephalin and ASPM expression are correlated
            with epithelial ovarian cancer progression
  JOURNAL   PLoS One 9 (5), e97059 (2014)
   PUBMED   24830737
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9727)
  AUTHORS   Xu,X.L., Ma,W., Zhu,Y.B., Wang,C., Wang,B.Y., An,N., An,L., Liu,Y.,
            Wu,Z.H. and Tian,J.H.
  TITLE     The microtubule-associated protein ASPM regulates spindle assembly
            and meiotic progression in mouse oocytes
  JOURNAL   PLoS One 7 (11), e49303 (2012)
   PUBMED   23152892
REFERENCE   5  (bases 1 to 9727)
  AUTHORS   Asami,M., Pilz,G.A., Ninkovic,J., Godinho,L., Schroeder,T.,
            Huttner,W.B. and Gotz,M.
  TITLE     The role of Pax6 in regulating the orientation and mode of cell
            division of progenitors in the mouse cerebral cortex
  JOURNAL   Development 138 (23), 5067-5078 (2011)
   PUBMED   22031545
REFERENCE   6  (bases 1 to 9727)
  AUTHORS   Desir,J., Cassart,M., David,P., Van Bogaert,P. and Abramowicz,M.
  TITLE     Primary microcephaly with ASPM mutation shows simplified cortical
            gyration with antero-posterior gradient pre- and post-natally
  JOURNAL   Am J Med Genet A 146A (11), 1439-1443 (2008)
   PUBMED   18452193
REFERENCE   7  (bases 1 to 9727)
  AUTHORS   Paramasivam,M., Chang,Y.J. and LoTurco,J.J.
  TITLE     ASPM and citron kinase co-localize to the midbody ring during
            cytokinesis
  JOURNAL   Cell Cycle 6 (13), 1605-1612 (2007)
   PUBMED   17534152
REFERENCE   8  (bases 1 to 9727)
  AUTHORS   Fish,J.L., Kosodo,Y., Enard,W., Paabo,S. and Huttner,W.B.
  TITLE     Aspm specifically maintains symmetric proliferative divisions of
            neuroepithelial cells
  JOURNAL   Proc Natl Acad Sci U S A 103 (27), 10438-10443 (2006)
   PUBMED   16798874
REFERENCE   9  (bases 1 to 9727)
  AUTHORS   Shen,J., Eyaid,W., Mochida,G.H., Al-Moayyad,F., Bodell,A.,
            Woods,C.G. and Walsh,C.A.
  TITLE     ASPM mutations identified in patients with primary microcephaly and
            seizures
  JOURNAL   J Med Genet 42 (9), 725-729 (2005)
   PUBMED   16141009
REFERENCE   10 (bases 1 to 9727)
  AUTHORS   Kouprina,N., Pavlicek,A., Collins,N.K., Nakano,M., Noskov,V.N.,
            Ohzeki,J., Mochida,G.H., Risinger,J.I., Goldsmith,P., Gunsior,M.,
            Solomon,G., Gersch,W., Kim,J.H., Barrett,J.C., Walsh,C.A.,
            Jurka,J., Masumoto,H. and Larionov,V.
  TITLE     The microcephaly ASPM gene is expressed in proliferating tissues
            and encodes for a mitotic spindle protein
  JOURNAL   Hum Mol Genet 14 (15), 2155-2165 (2005)
   PUBMED   15972725
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On or before Oct 27, 2010 this sequence version replaced
            NM_001198563.1, NM_001105955.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.49328.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-403               JAXUCZ010000013.1  53625584-53625986
            404-547             JAXUCZ010000013.1  53627557-53627700
            548-1994            JAXUCZ010000013.1  53627856-53629302
            1995-2099           JAXUCZ010000013.1  53632046-53632150
            2100-2246           JAXUCZ010000013.1  53632388-53632534
            2247-2492           JAXUCZ010000013.1  53633653-53633898
            2493-2560           JAXUCZ010000013.1  53634663-53634730
            2561-2702           JAXUCZ010000013.1  53638807-53638948
            2703-2833           JAXUCZ010000013.1  53639456-53639586
            2834-3009           JAXUCZ010000013.1  53640163-53640338
            3010-3155           JAXUCZ010000013.1  53641411-53641556
            3156-3241           JAXUCZ010000013.1  53641647-53641732
            3242-3463           JAXUCZ010000013.1  53642535-53642756
            3464-3671           JAXUCZ010000013.1  53644295-53644502
            3672-3814           JAXUCZ010000013.1  53644595-53644737
            3815-3943           JAXUCZ010000013.1  53644960-53645088
            3944-4138           JAXUCZ010000013.1  53654328-53654522
            4139-7918           JAXUCZ010000013.1  53659792-53663571
            7919-8085           JAXUCZ010000013.1  53664843-53665009
            8086-8179           JAXUCZ010000013.1  53666253-53666346
            8180-8389           JAXUCZ010000013.1  53667776-53667985
            8390-8539           JAXUCZ010000013.1  53669000-53669149
            8540-8731           JAXUCZ010000013.1  53670318-53670509
            8732-8924           JAXUCZ010000013.1  53670633-53670825
            8925-9079           JAXUCZ010000013.1  53670962-53671116
            9080-9256           JAXUCZ010000013.1  53672409-53672585
            9257-9426           JAXUCZ010000013.1  53673251-53673420
            9427-9727           JAXUCZ010000013.1  53674189-53674489
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q13"
     gene            1..9727
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /note="assembly factor for spindle microtubules"
                     /db_xref="GeneID:289054"
                     /db_xref="RGD:1307111"
     exon            1..403
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..9532
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /note="asp (abnormal spindle)-like, microcephaly
                     associated; calmodulin binding protein 1; asp (abnormal
                     spindle) homolog, microcephaly associated; abnormal
                     spindle microtubule assembly"
                     /codon_start=1
                     /product="abnormal spindle-like microcephaly-associated
                     protein"
                     /protein_id="NP_001099425.2"
                     /db_xref="GeneID:289054"
                     /db_xref="RGD:1307111"
                     /translation="MATVQAAYSSEESGRRARRDPAAEDASPPVLLLSHFCAVPFLCF
                     GDVRVGTSRTRSLVLHNPHAEPLHVELSLLRAAGQGFSASPNRCELQPKEKITISVTW
                     TPLKEGGVREIVTFLVNDFLKHQAVLLGNAEEQRKKKRSLWNTSKKIPASSKYTKRTS
                     KNQNFNESFTISQKVDRIRSPLQPCENLAMSECCSPTENSSLPLEENKVPTSSISPIR
                     ECQSEACLSLFSPGPTAYSSLHESENARNSKVQDASISKNFDFNEEVTNETFINPISV
                     SHHSEGDSKLALTPNCSSPLNIAQTQMHFLSPDSFENDSYTSDNGLRSMKNVLSDTFK
                     KDPSESVCLESQTVHEVCQTILSPESFLNDNYGLKKGLNLESVNPVLSPTQFVKDNMV
                     HVGQQMGELSPLPAKPSQDRRVNEGLADNPKGQHAQTPSSKSEQQNPGEMNLHNYGFT
                     KQKPKISELQDAFCNQSKQLKKRRPILSATVTKRKPTNAREKLPEINKPNAKRSLEGV
                     VGECGKEVDKLKEKAFHSSLPVVEAVINKPPSYRNEVTPSTVVIARKRKSSGTVGDTS
                     GKLAVAEWMDVCEIKRIHFSPLESTPSTVARTTKKEALTSNRISNLERSGLKKKTDSS
                     ILKTPLSKTKRKRRSIVPVAQSHLTFIKPLKAAIPRHPMPFAAKNMFYDERWKEKQEQ
                     GFTWWLNYILTPDDFTVKTNVSKVNAATLILGAEGQHKVSVPKAPTKEEVSLRAYTAS
                     CRLNRLRRTACSLFTSEKMVKAIKKIEIEIEVGRLLVRKDRHLWKDIGQRQKVLNWLL
                     SYNPLWLRIGLETVFGELIPLADNSDITGLAMFILNRLLWNPDIAAEYRHPTVPLLFR
                     DGHEAALSKFTLKKLLLLICFLDHAKISRLIDHDPCLFCKDAEFKASKELLLAFSRDF
                     LSGEGDLSRHLSLLGLPVSHVQTPLDEFDFAVTNLAVDLQCGVRLVRTIELLTQNWNL
                     SDKLRIPAISRVQKMHNVDLVLQVLKSRGVSLTDEHGNTILSKDIVDRHREKTLGLLW
                     KIAFAFQVDISLNLNQLKEEIDFLKHTHNIKTAMSALICSSPAVTKKQKDRRFSGNFE
                     HYGDSVKLLMDWVNAVCAFYNKKVENFTVSFSDGRVLCYLIHHYHPGYVPFDAICQRT
                     TQSVACTQTGSVVLNSSSESEGGCLDLSLEALDHESTPEMYKELLENEKKNFHLVRSA
                     ARDLGGIPAMIHHADMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQITWRKYK
                     LKRDLKDRQERDKAARIIQAVVLNFLTRRRLQKRVSAALVIQTCWRRVSAQRKLLMLK
                     NEKLAKLHSASASLIQAHWRRHSTRKHFLKLKHCSVILQSRLRMKIALTSYKRYRWAT
                     VTIQRRWRAHLSRKRDQQRFRKLKSASLVIQSVFRRWKRRKLQLQTKAAVTLQRAFRE
                     WRCRKQTRERAAVVIQSWYRMHRELQKYIYIRSCVVVIQRRVRCFHAQKLCKRRKDAV
                     LTLQKHFRARQMGKLARTDYLQKRAAAIQLQAAFRGMKARRSYRLQIQAACVLQSYWR
                     MRQGRVKFLNLKKTVIKLQAHIRKHQQLQKYQKIKKAAVTIQTHFRASISARKSLASY
                     QKTRSSVIVLQSAYRGMQARKTFRRALTSVVKIQSCYRAYISRKKFRSLRNAAVKLQS
                     IVKMKQSRKQYLQVRAAAVFIQRWYRSQKRKESVQVREACIKLQSYFRGCLVRKQMRL
                     QSKAAISLQSYFRMWRVRQRYLKTRKAALVIQTFYSAYRAQVHQRKIFLQVKGAAVCL
                     QAAYRGRRARRQIKQQSTAAVTIQRVFRGHSQRMKYQTVLQSAVKVQRWYRAHKVASD
                     MRIHFLKTREAVVVLQSACRGWQVRQQLRRQHQAAVKIQAAFRMARAQRQYKLVRNAA
                     VVIQQHVRARAAGERQHSEYIRLRHAALVFQATWKGKMLRREIAQRHQCAALIQSYYR
                     MYSQRRKWSLMKTAARQIQVCYRAYRVGKEQRHLYLKTKAAVVILQSAYRGMKARRQI
                     TECHKAAVTIQSKFRAYRTQKKYTTCRTSAIIIQRWYRNIKITTQQREAYLTLKRAAT
                     KVQAVYRGIQVRRHIQHMYMAATLIKALFKIQQSRAAAVIQVRYRAYYLGKIQQEKYL
                     TTLRAIKTLQAGVRGARVRKTLRKMHFAATLIQSYFRRHRQRTYFHRLRKAATVVQQR
                     YRAVKEGSSQFQRYSRLRRSVILVQAAFRGLKARRHLKAMHLAATVIQRRFRTLLMRR
                     KFLSLRKTVIWIQRQYRARLHAKYCRQQLLLEKAVTKIQSSYKGWMVRKSVQKMHRAA
                     TVIQATFRMHRACVRYQRLKRASVVIQKQYRAHRTAELQRQLFVRQRQAALTLQAAFR
                     GVKARNHLKTMHASATLIQSKFRARIVRRQFLALRKAAIFVQRKYRATLYAKHKLHQF
                     LQLRKAAITIQSSFRRLMVKKQLQERHRAAALIQATFRMHRTYVRFHLWKRASIRIQQ
                     HYRTYRTRKLQREQLSREEHRAAVRIQSTYRMHRQRCVYQQLRWAAKVIQKKYRANKR
                     RQDLPMACKEETPLTQMHFQGSNMAKQVQLQHDAAVIIQKHFRAFKARRLVEAERGSQ
                     AVWRTSEARQDLRRGEAACRIQAWYRRCRVRRRYLTLLKAVKTIQGYFYAKQERTRFL
                     KMRAAAIIIQRKWRAALCARAACESLTLKRHGAACVIQAHFRGYKGRQSFRQQRSAAL
                     TIQRYVRASVAARQERTKYVQFKKSAVVLQALVRGWLARKRVSEQKANIRRFRFIAAA
                     YCHMCALRIQRAYRRHVAMRNAKKHVNSVIVIQRWFRRRLQQQRFIEQYRKIMAAERD
                     VQARRHQQDRAASVIQKAVRRFLLSRRQQKVNSSATRIQALWRGYASRKKNDRTEIKA
                     IRQSLRAVSRNVEEENKLYRRTERALHHLLTYKHLSAILEAMKHLEVVTRLSPLCCEN
                     MAESGAVSTVFVVIRSCNRSVPCMEVVGCAVQVLLNVAKYDKTISAVYDAENCVDTLL
                     ELLQAYREKPGDRVAEKSASIFTRTCCLLAVLLKTEQCAFDAQSRSKVIDRIYRLYKF
                     TVPKHKVNTQGLFDKQKQNSCIGFPFIPERSIKTRIVSRLKPQWVLRRDNMEEITNSL
                     QAIQLVMDTLGIPYC"
     exon            404..547
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            548..1994
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2099
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2100..2246
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2492
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..2560
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2702
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2833
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            2834..3009
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3010..3155
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3156..3241
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3242..3463
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3464..3671
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..3814
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3815..3943
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            3944..4138
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            4139..7918
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            7919..8085
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            8086..8179
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            8180..8389
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            8390..8539
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            8540..8731
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            8732..8924
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            8925..9079
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            9080..9256
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            9257..9426
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
     exon            9427..9727
                     /gene="Aspm"
                     /gene_synonym="Calmbp1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggctaggc tactttcccg ggagtttggg gatatccctg ttcttgtgat ctttgtcctg
       61 gtccttggct tccgggaggc cggcgggacc cgagcagcgg gtacctgact gcccggcgcg
      121 ggcggctgcc atggcgacgg tgcaggcggc ttacagctcg gaggagagcg ggcgacgggc
      181 gcgccgggat cccgcggccg aggacgcgtc tccgccggtg ctattgctca gccacttctg
      241 cgccgttccc ttcctctgtt tcggggatgt ccgcgtgggt acgtcgcgta cgcggtctct
      301 ggtcctgcac aacccgcacg cggagcctct gcatgtggag ctgtcgctgc tgcgcgccgc
      361 aggccagggc ttcagcgcgt cgccgaaccg ctgcgagctg cagcctaaag aaaagattac
      421 catttctgtt acctggacac cactgaaaga agggggagta agggagattg tgacatttct
      481 tgtaaatgat tttctgaagc accaggctgt attgttagga aatgcagaag agcagaggaa
      541 gaaaaagaga agtctttgga ataccagtaa gaagattcca gcctcttcaa aatataccaa
      601 aaggacctcc aaaaaccaaa attttaatga atcgtttact atttcacaaa aagttgacag
      661 aattaggagc ccactgcagc cttgtgaaaa tctggctatg agtgaatgct gctccccaac
      721 agaaaacagt tctttacccc ttgaagaaaa caaagtgccc acctcatcca tcagccctat
      781 tagagaatgc cagagtgaag cttgcttgtc actgttttcc cccggaccca ctgcctattc
      841 gtctcttcat gaatccgaaa atgcacgaaa ttcaaaagta caagatgcca gcatttcaaa
      901 aaattttgat tttaacgagg aagttacaaa tgaaactttt attaatccca ttagcgttag
      961 tcaccacagt gagggggata gtaaacttgc tctgacccca aactgttctt cgcctttgaa
     1021 tattgcacaa acccaaatgc attttttaag tccagattct tttgaaaacg acagctatac
     1081 atctgataat ggcctgagat caatgaaaaa tgttttatca gatacattta agaaagatcc
     1141 ctcagaatct gtatgtctgg aatcccaaac tgtacatgaa gtttgccaaa caatcttaag
     1201 cccagagtct tttctaaatg ataattatgg acttaagaaa ggtctaaact tagaatccgt
     1261 caatcctgtt ttatctccaa cccaatttgt aaaggataac atggtccacg tagggcagca
     1321 aatgggtgag ctatcaccat tgccagctaa accgtctcag gaccggagag taaatgaagg
     1381 cttggcggat aaccccaagg gtcagcatgc acaaactccc agcagtaaat ctgaacaaca
     1441 aaaccctgga gaaatgaatt tacataacta tggttttaca aagcaaaaac ccaagatttc
     1501 tgagcttcaa gatgcttttt gtaaccagag caaacaactg aaaaagagac gtccaattct
     1561 ctccgccact gttaccaaaa ggaagcccac caatgccaga gaaaagctac ctgagattaa
     1621 caaaccaaat gcaaaaagga gtctcgaagg tgtagtagga gaatgtggaa aagaagtgga
     1681 taagctaaaa gagaaagcct ttcattcttc tcttccagtt gtagaggcag taataaataa
     1741 acctccaagt tatagaaatg aagtaacacc ttcaactgtt gtaatcgccc gcaaaagaaa
     1801 gagcagtggg accgtgggag acacaagtgg aaagctggct gttgcagaat ggatggacgt
     1861 gtgtgaaatc aaacggatcc atttttctcc tctggaatct acaccatcaa ctgtggcaag
     1921 aaccacaaag aaagaagcac tcacttcaaa tcgtataagc aacttggagc gatcaggcct
     1981 gaagaagaaa acagattcat caatattaaa aacacctctt tctaaaacaa agagaaagag
     2041 aagatccatt gtccctgtag cacagtctca tctgaccttt ataaagccat taaaagcagc
     2101 tattcctaga cacccaatgc catttgctgc aaagaatatg ttctatgatg aacgctggaa
     2161 ggaaaaacag gaacagggct tcacttggtg gctaaattat atattaacac ctgatgactt
     2221 cactgtaaaa accaatgttt ctaaagtaaa cgcggccacc cttattttgg gagcggaggg
     2281 ccagcataaa gtaagtgtcc cgaaagcacc tacaaaagag gaagtgtctc tcagagctta
     2341 caccgccagc tgcaggctga atagattacg tcgtacagct tgctccttgt ttacttctga
     2401 aaaaatggtt aaagctatta aaaagattga aattgaaatc gaagttgggc ggctacttgt
     2461 tcgaaaagat cgacacctct ggaaagatat tggacaacgc caaaaagtac tgaattggct
     2521 cttgtcatat aatcctctgt ggcttcgaat tggtctagag acagtttttg gagaactgat
     2581 tcctctcgca gacaacagtg acatcacagg attggcgatg ttcattctga atcgcttact
     2641 ttggaatcct gacatagcgg ctgaatatag acaccccact gtccctctcc tgtttcggga
     2701 tggtcatgaa gcagctttgt ctaagttcac attgaaaaag ttactgttgt tgatctgttt
     2761 ccttgaccat gctaaaattt ccagacttat tgatcatgat ccgtgtctct tttgtaaaga
     2821 tgctgaattc aaggctagta aagagctcct tctagccttt tcacgagact tcctaagtgg
     2881 tgaaggtgac ctttctcgtc accttagctt attggggttg cctgtgagcc acgttcagac
     2941 accacttgat gaatttgatt ttgctgttac gaatcttgca gtggacttgc agtgtggggt
     3001 tcgccttgtg cgaactatag agcttctgac ccagaactgg aatctgtcag acaaactcag
     3061 gattcctgca ataagtcgag ttcaaaagat gcacaatgtt gatcttgttc ttcaggttct
     3121 caaatcacga ggagtttctc tgacggatga gcacggaaac actatcttgt ctaaggatat
     3181 cgtggatagg cacagagaga aaacccttgg gttgctttgg aaaatagcat ttgctttcca
     3241 ggtggatatt tcccttaatt taaatcaact aaaggaagaa attgattttc taaagcacac
     3301 acacaatata aagacagcta tgtctgcact aatatgctct tctccagcgg ttactaagaa
     3361 gcaaaaagac agacggttta gtgggaactt tgaacactat ggtgacagtg tgaagctatt
     3421 gatggactgg gtaaatgcag tttgcgcctt ctataataaa aaggtggaga attttacagt
     3481 gtctttctca gatggccgtg tgctatgcta tttgatccac cattaccacc cgggctatgt
     3541 tcctttcgat gctatatgcc agcgcaccac tcagtccgtg gcctgcacac agactggttc
     3601 agtggtattg aattcctcat ccgaatctga aggcggttgt ctggatctgt ctctggaagc
     3661 acttgatcat gaaagtactc cagaaatgta caaagagctt ctagaaaacg aaaagaaaaa
     3721 ttttcatctg gtgagatctg cagctagaga tcttggagga atacctgcta tgattcatca
     3781 tgcagatatg tcaaatacca tcccagacga gaaggtggtt atcacctact tgtcatttct
     3841 ttgtgcacgg cttttggatc tccgtaaaga aataagagct gcccgactta tacagataac
     3901 ctggagaaaa tataaactaa aaagagacct gaaagaccgt caggaaagag acaaagctgc
     3961 tagaattatt caggcagtcg ttctcaattt tctaactaga agaagattgc aaaagagagt
     4021 cagtgcagcg ctcgtcattc aaacatgttg gcgaagagtc tcagcacaga ggaagttgct
     4081 gatgctgaag aatgaaaaac tggcaaaact tcacagtgca tcagcatcac tgattcaggc
     4141 tcactggaga agacactcca ccagaaaaca cttcctgaag ttgaaacact gttccgtcat
     4201 ccttcagtcc aggttaagaa tgaaaatcgc ccttacatct tacaagcgat atcgttgggc
     4261 cacggttacc atccagaggc gttggcgcgc ccatttaagt agaaaacgag atcagcagag
     4321 attcagaaag ctgaaatctg catcccttgt aatccagtct gtgttcagac ggtggaagcg
     4381 acgcaagctg cagctccaaa ccaaagccgc ggtgacattg cagcgagctt ttagagaatg
     4441 gcgttgcagg aaacagacac gagaaagggc cgctgttgtg atacagtcgt ggtacagaat
     4501 gcacagggaa ttgcagaagt acatttacat taggtcttgt gttgtagtca tccagagaag
     4561 agttcggtgc tttcacgccc aaaagttatg taagagaagg aaagacgctg tcctgactct
     4621 ccagaagcac ttcagggccc gtcagatggg gaagttagcg cgcactgact atttgcagaa
     4681 gcgagcggct gccattcaac tgcaggctgc cttcaggggc atgaaggctc gccgttcata
     4741 ccgtttgcaa attcaagctg cctgtgttct tcagtcctat tggagaatga gacagggaag
     4801 agttaaattc ttaaatctta aaaagactgt tatcaaattg caagcacaca taagaaaaca
     4861 ccaacaacta cagaaatatc agaagataaa gaaagcagct gttacaattc agactcattt
     4921 ccgagcttcc atttcagcca ggaagagtct agcatcttac cagaaaacaa ggtcttcagt
     4981 cattgttcta cagtctgcat atagagggat gcaagccagg aaaacatttc gtcgtgcttt
     5041 aacatctgta gtaaagattc agtcatgtta tcgggcttat atctccagaa agaagtttcg
     5101 gagcttgagg aatgctgcag taaagctgca gtcaattgtc aaaatgaaac agtcacgtaa
     5161 gcagtattta caggtaagag ccgctgcggt atttatccag cggtggtacc gttcccaaaa
     5221 gagaaaagag tctgtacagg tgcgcgaagc ctgtatcaaa ctgcagtcgt acttcagagg
     5281 atgcctggtc agaaagcaga tgaggctgca aagcaaggct gccatttctc tgcagtctta
     5341 cttcaggatg tggagagtga ggcagcgcta cctgaagacg cgcaaggcgg ctcttgtcat
     5401 tcagactttc tacagtgcct accgagcaca agtccatcag aggaagatct tcctgcaggt
     5461 caaaggagca gctgtttgtt tgcaagcagc ttacagaggc cgcagagcac gccggcaaat
     5521 caagcagcag tctacagctg ccgttacaat tcagagggtt tttagaggcc acagtcagag
     5581 gatgaaatac cagactgtgc ttcagtctgc tgtgaaggtt cagagatggt acagggcaca
     5641 caaggttgct tctgacatga gaattcactt tctaaagaca agggaggctg tcgttgttct
     5701 gcagtctgcc tgccgtggct ggcaagttcg gcagcagctc aggaggcaac atcaggctgc
     5761 agtgaagatc caggctgctt tcagaatggc cagggcccag aggcagtata aactagtgag
     5821 aaatgctgca gtcgtcatcc agcagcatgt cagggcacgg gctgcaggag agaggcagca
     5881 ctcggagtac atccggctcc gtcacgccgc cctggtgttt caggctacgt ggaagggaaa
     5941 gatgctgagg agagagatcg cacagcggca ccagtgcgcg gccctcatac agtcgtacta
     6001 cagaatgtac agtcagcgta ggaagtggag cctcatgaaa accgctgctc gtcagattca
     6061 ggtgtgctat agggcctaca gagttggaaa ggaacagcgc catttgtact tgaaaacaaa
     6121 ggcagctgta gtaattttac agtcagctta ccgtggtatg aaagcgagaa gacagataac
     6181 tgaatgccac aaagcagcag tcactataca gtctaaattt agggcctaca gaacacagaa
     6241 gaaatataca acctgtagaa cttctgctat tataattcag agatggtatc ggaatattaa
     6301 aattaccacc cagcaacgtg aggcatacct taccttaaag agagcagcaa ctaaagtcca
     6361 agctgtgtac agaggcattc aagtaaggag acacattcag cacatgtaca tggcggccac
     6421 actcattaaa gccttgttta agatacagca gtcgagagca gcggctgtga tccaggtgag
     6481 gtacagagcg tattatctag gtaaaattca gcaggaaaag tacctgacga ctttaagggc
     6541 tattaagacc cttcaagccg gtgtcagagg agcaagagtt agaaagactt taagaaagat
     6601 gcatttcgca gcaacgctca ttcagtcata ctttaggaga cacagacagc gaacatattt
     6661 ccaccgcttg aggaaggcag ccacagtggt gcagcagagg taccgagcag taaaggaagg
     6721 aagctctcag tttcagaggt acagcagact gaggcgttct gtaatcctcg tccaggctgc
     6781 ctttagggga ctgaaagcgc ggagacactt aaaagctatg catttagctg ctactgtcat
     6841 tcagaggaga ttcaggacac tactgatgag aaggaagttc ctctctctca ggaaaacagt
     6901 catttggatt cagagacagt accgtgcaag acttcacgcc aagtattgcc gacaacaatt
     6961 actgttggag aaggcggtta caaagatcca gtcatcatac aaaggatgga tggtaaggaa
     7021 aagtgtgcag aagatgcaca gggctgctac tgtgattcag gccactttcc gaatgcacag
     7081 agcctgtgtg aggtaccagc gtttgaagag ggcctcagtt gtgatccaga agcaataccg
     7141 agcacacaga actgcagaac tgcagaggca gctttttgtc aggcagagac aagctgctct
     7201 gacccttcag gctgccttta ggggcgtgaa agccagaaac catttgaaga ctatgcatgc
     7261 ttctgcaacc cttattcaga gtaaatttag agctagaata gtaaggagac aattccttgc
     7321 tctcagaaaa gctgctatat tcgtgcagag gaaatacaga gccacccttt atgccaagca
     7381 taaattgcac caattcttgc agttacgaaa ggcagccatt acaatacagt cctcttttcg
     7441 aagactgatg gtcaaaaagc agttacaaga aaggcacagg gctgcggctc tcatccaggc
     7501 tacgtttaga atgcacagaa cttacgttag gtttcacctt tggaagcgtg cctcaattag
     7561 aattcaacag cattatcgaa catacagaac tagaaaactg caaagagaac aactcagcag
     7621 agaagaacac cgtgctgctg tcagaataca aagcacatac aggatgcata ggcagcgctg
     7681 tgtctaccag cagcttcggt gggcagcaaa agtgatacaa aagaaataca gagcaaataa
     7741 gagacggcaa gatctgccca tggcatgtaa ggaagaaaca cctcttactc agatgcattt
     7801 tcagggctca aacatggcca aacaagttca gctgcagcat gacgctgctg tgattataca
     7861 gaagcatttt agagccttta aagcaaggag gctggtggag gctgaaagag gctcccaggc
     7921 tgtgtggaga acatctgaag ctcggcagga cttacgcaga ggggaagctg cctgcaggat
     7981 tcaagcttgg tataggcgct gcagagtgcg caggagatac ctaactttat tgaaagctgt
     8041 taaaaccatc caaggttact tctatgcgaa gcaggagaga acgaggttct tgaagatgag
     8101 ggcagcggct atcataattc aaaggaagtg gagagctgca ctctgtgcaa gagcagcatg
     8161 tgagagtttg accttaaaaa gacatggagc cgcttgtgtg atccaggccc actttagagg
     8221 ctataagggg aggcagtcct tccgacagca gaggtctgct gctttaacca tacagagata
     8281 tgtacgagcc tcggtggctg cgaggcagga gaggacaaag tacgtccagt ttaagaagtc
     8341 tgcagttgtt ttacaggcat tggtgcgtgg ctggttagca agaaaaagag tttcagaaca
     8401 gaaagccaac attcgacgtt tccgctttat agcagctgca tactgtcaca tgtgtgctct
     8461 tagaatccag cgagcttata gacgccacgt ggctatgagg aatgctaaga agcatgttaa
     8521 ttcagtcatc gttattcaga gatggtttcg aagaagatta cagcagcaga ggttcattga
     8581 gcaatatcgt aagatcatgg cagctgagcg tgacgttcaa gctcgtcggc accagcagga
     8641 cagggctgca tcagtgattc agaaggcagt gcgccgcttt ctcctgagca gaagacagca
     8701 aaaggtcaac agcagcgcca ctagaattca ggcattatgg agaggctatg cttcaaggaa
     8761 gaaaaatgat cgcacagaaa ttaaagctat tcgacaaagc cttcgcgctg tcagcaggaa
     8821 cgtggaggag gaaaacaagc tgtacagaag aactgagcgt gcccttcatc acctgctcac
     8881 gtacaagcac ttgtcggcca ttctggaagc catgaagcac ctggaggttg ttaccaggtt
     8941 atctcccctt tgctgtgaga acatggctga aagcggagca gtgtctaccg tcttcgtggt
     9001 gatccgcagc tgtaatcgca gtgttccttg catggaagtt gttggatgtg ctgtccaagt
     9061 cctgctgaat gtggcaaagt atgataagac gatctcagct gtttacgatg ctgaaaactg
     9121 tgtggacaca ctgctggagc tgctgcaggc gtaccgggag aagcctggtg accgagttgc
     9181 cgagaagagt gccagcattt tcacaaggac gtgctgttta ttagctgtgc tgttgaagac
     9241 agagcagtgt gcttttgatg cacaaagtag atcaaaagtc attgatcgta tttatcgtct
     9301 ctacaaattt acagttccta agcataaagt gaatacacag ggattatttg ataagcagaa
     9361 gcagaattcc tgtataggct ttccttttat accagaaaga tctataaaga ccaggatagt
     9421 ttcaagactt aagccacagt gggttttgag gcgtgataat atggaagaaa tcacaaattc
     9481 cctgcaagct attcaactgg tgatggatac ccttggaatt ccttattgct aaaaggaaac
     9541 attttcagtg tgggaccaaa aaaaaaactt atgcaatcat ggatacctgg agcagttttt
     9601 actgtcagtg taaactgttt agatatgact ttgtgtaaaa ataaatatgt atatattaca
     9661 agttactaag ttgtttaatg aattcatttt tactgttttt aatttttgag attataattt
     9721 aaataca
//
