LOCUS       NM_024399               1565 bp    mRNA    linear   ROD 12-AUG-2024
DEFINITION  Rattus norvegicus aspartoacylase (Aspa), mRNA.
ACCESSION   NM_024399 XM_006246815
VERSION     NM_024399.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Takeda,S., Hoshiai,R., Tanaka,M., Izawa,T., Yamate,J., Kuramoto,T.
            and Kuwamura,M.
  TITLE     Myelin lesion in the aspartoacylase (Aspa) knockout rat, an animal
            model for Canavan disease
  JOURNAL   Exp Anim 73 (3), 347-356 (2024)
   PUBMED   38538326
  REMARK    GeneRIF: Myelin lesion in the aspartoacylase (Aspa) knockout rat,
            an animal model for Canavan disease.
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Nishitani,A., Tanaka,M., Shimizu,S., Kunisawa,N., Yokoe,M.,
            Yoshida,Y., Suzuki,T., Sakuma,T., Yamamoto,T., Kuwamura,M.,
            Takenaka,S., Ohno,Y. and Kuramoto,T.
  TITLE     Involvement of aspartoacylase in tremor expression in rats
  JOURNAL   Exp Anim 65 (3), 293-301 (2016)
   PUBMED   27026062
  REMARK    GeneRIF: Interaction of N-acetyl-L-aspartate accumulation due to
            ASPA deficiency with an unstable neuronal membrane potential caused
            by HCN1 deficiency was involved in tremor development.
REFERENCE   3  (bases 1 to 1565)
  AUTHORS   Di Pietro,V., Amorini,A.M., Tavazzi,B., Vagnozzi,R., Logan,A.,
            Lazzarino,G., Signoretti,S., Lazzarino,G. and Belli,A.
  TITLE     The molecular mechanisms affecting N-acetylaspartate homeostasis
            following experimental graded traumatic brain injury
  JOURNAL   Mol Med 20 (1), 147-157 (2014)
   PUBMED   24515258
  REMARK    GeneRIF: The decrease in N-acetylaspartate following severe
            traumatic brain injury was characterized by profound mitochondrial
            malfunctioning, dramatic repression of Nat8l gene and concomitant
            increase in the ASPA gene and protein levels.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1565)
  AUTHORS   Benesh,A.E., Fleming,J.T., Chiang,C., Carter,B.D. and Tyska,M.J.
  TITLE     Expression and localization of myosin-1d in the developing nervous
            system
  JOURNAL   Brain Res 1440, 9-22 (2012)
   PUBMED   22284616
REFERENCE   5  (bases 1 to 1565)
  AUTHORS   Moffett,J.R., Arun,P., Ariyannur,P.S., Garbern,J.Y.,
            Jacobowitz,D.M. and Namboodiri,A.M.
  TITLE     Extensive aspartoacylase expression in the rat central nervous
            system
  JOURNAL   Glia 59 (10), 1414-1434 (2011)
   PUBMED   21598311
  REMARK    GeneRIF: This study demonistrated that aspartoacylase expression in
            the rat white matter central nervous system.
REFERENCE   6  (bases 1 to 1565)
  AUTHORS   Francis,J.S., Olariu,A., McPhee,S.W. and Leone,P.
  TITLE     Novel role for aspartoacylase in regulation of BDNF and timing of
            postnatal oligodendrogenesis
  JOURNAL   J Neurosci Res 84 (1), 151-169 (2006)
   PUBMED   16634055
REFERENCE   7  (bases 1 to 1565)
  AUTHORS   McPhee,S.W., Francis,J., Janson,C.G., Serikawa,T., Hyland,K.,
            Ong,E.O., Raghavan,S.S., Freese,A. and Leone,P.
  TITLE     Effects of AAV-2-mediated aspartoacylase gene transfer in the
            tremor rat model of Canavan disease
  JOURNAL   Brain Res Mol Brain Res 135 (1-2), 112-121 (2005)
   PUBMED   15857674
REFERENCE   8  (bases 1 to 1565)
  AUTHORS   Madhavarao,C.N., Moffett,J.R., Moore,R.A., Viola,R.E.,
            Namboodiri,M.A. and Jacobowitz,D.M.
  TITLE     Immunohistochemical localization of aspartoacylase in the rat
            central nervous system
  JOURNAL   J Comp Neurol 472 (3), 318-329 (2004)
   PUBMED   15065127
  REMARK    GeneRIF: ASPA colocalized throughout the brain with CC1, a marker
            for oligodendrocytes, with 92-98% of CC1-positive cells also
            reactive with the ASPA antibody.
REFERENCE   9  (bases 1 to 1565)
  AUTHORS   Kirmani,B.F., Jacobowitz,D.M. and Namboodiri,M.A.
  TITLE     Developmental increase of aspartoacylase in oligodendrocytes
            parallels CNS myelination
  JOURNAL   Brain Res Dev Brain Res 140 (1), 105-115 (2003)
   PUBMED   12524181
  REMARK    GeneRIF: The develomental increase of this enzyme in
            oligodendrocytes parallels CNS myelination.
REFERENCE   10 (bases 1 to 1565)
  AUTHORS   Kaul,R., Gao,G.P., Balamurugan,K. and Matalon,R.
  TITLE     Cloning of the human aspartoacylase cDNA and a common missense
            mutation in Canavan disease
  JOURNAL   Nat Genet 5 (2), 118-123 (1993)
   PUBMED   8252036
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On or before Nov 24, 2020 this sequence version replaced
            XM_006246815.3, NM_024399.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078813.1, SRR26360176.1832146.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-399               JAXUCZ010000010.1  58438639-58439037   c
            400-595             JAXUCZ010000010.1  58434469-58434664   c
            596-689             JAXUCZ010000010.1  58433279-58433372   c
            690-797             JAXUCZ010000010.1  58426900-58427007   c
            798-907             JAXUCZ010000010.1  58422174-58422283   c
            908-1565            JAXUCZ010000010.1  58418595-58419252   c
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q24"
     gene            1..1565
                     /gene="Aspa"
                     /note="aspartoacylase"
                     /db_xref="GeneID:79251"
                     /db_xref="RGD:621693"
     exon            1..399
                     /gene="Aspa"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..1105
                     /gene="Aspa"
                     /EC_number="3.5.1.15"
                     /note="ACY-2; aminoacylase-2"
                     /codon_start=1
                     /product="aspartoacylase"
                     /protein_id="NP_077375.2"
                     /db_xref="GeneID:79251"
                     /db_xref="RGD:621693"
                     /translation="MTSCVAEEPIKKIAIFGGTHGNELTGVFLVTHWLKNGAEVHRAG
                     LEVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEMSEDLPYEVRRAQEINHLFGPK
                     NSDDAYDVVFDLHNTTSNMGCTLILEDSRNDFLIQMFHYIKTCMAPLPCSVYLIEHPS
                     LKYATTRSIAKYPVGIEVGPQPHGVLRADILDQMRRMLKHALDFIQRFNEGKEFPPCA
                     IDVYKIMEKVDYPRNESGDVAAVIHPNLQDQDWKPLHPGDPVFVSLDGKVIPLGGDCT
                     VYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRSTLH"
     exon            400..595
                     /gene="Aspa"
                     /inference="alignment:Splign:2.1.0"
     exon            596..689
                     /gene="Aspa"
                     /inference="alignment:Splign:2.1.0"
     exon            690..797
                     /gene="Aspa"
                     /inference="alignment:Splign:2.1.0"
     exon            798..907
                     /gene="Aspa"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1565
                     /gene="Aspa"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tggacattgt gacagaaaca gttgtgaagt cagttcccaa gagagctctg tatttttcac
       61 ttctcactta gatctaaact tggaaacttt tctcagaagt taagtgtcct ttgacctcct
      121 ctcttctgaa ttgcagaaac cagaccagat tttggtattt ggtaaaatga cttcttgtgt
      181 tgctgaagaa cctattaaaa agattgccat cttcggaggg actcatggaa atgaactgac
      241 tggagtgttt ctagttactc actggctaaa gaatggcgct gaagttcaca gagcagggct
      301 ggaagtgaag ccattcatta ccaacccaag agcggtggag aagtgcacca gatacattga
      361 ctgtgacctg aaccgtgttt ttgaccttga aaatcttagc aaagagatgt ctgaagattt
      421 gccgtatgaa gtgagaaggg ctcaagaaat aaatcactta tttggtccaa aaaatagtga
      481 tgatgcctac gatgtcgttt ttgaccttca taacactact tctaacatgg gttgcactct
      541 tattcttgag gattccagga atgacttttt aatccagatg tttcactata ttaagacctg
      601 catggctcca ttaccctgct ctgtttacct catcgagcat ccttccctca aatatgcaac
      661 cactcgttcc attgccaagt atcctgttgg tatagaagtt ggtcctcagc ctcacggtgt
      721 tctgagagct gatattttag accaaatgag aagaatgctc aagcacgctc ttgattttat
      781 acagcgtttc aatgaaggta aagaatttcc cccctgtgct attgatgtct ataaaataat
      841 ggagaaagtt gattatccaa ggaacgaaag tggagacgtg gctgctgtta tccatcctaa
      901 tctgcaggat caagactgga aaccattgca ccctggagat cctgtgtttg tgtctcttga
      961 tggaaaagtt attccactgg gtggagactg taccgtgtac ccagtgtttg tgaatgaagc
     1021 tgcatattat gaaaaaaaag aagcatttgc aaagacaaca aaactaacac tcaacgcaaa
     1081 aagcatccgc tccactttgc actaaacatc tctgcaagct cacagttatg ggatcttaca
     1141 gacctctaga agtctgtaaa cgctccaaga ccagagtgcc tcattctagt tcttttcctc
     1201 agcacctggc cccatgcctc aagcagtaag catccaggcc aggttctgaa aggaaagaat
     1261 aaattcatga atgtctttca tatatgtttg gatgagctat gtacagtaga caggcaagtg
     1321 tgcttctggt ttctgtatta ttctttatac attacattct ggtagtttaa cattgttaac
     1381 tttcacattt agaacataac attgaaatac atgctacatg caatttagca tacagtgaga
     1441 cagactgaat atgtgttttc aaggaatgca taagtactat tcaaaactaa tagtacagac
     1501 ttgctgtttt taagtgctga tatgtgagtt aataacagtg tattattaaa tgtccattcc
     1561 ttaaa
//
