LOCUS       NM_013079               2040 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus asparagine synthetase (glutamine-hydrolyzing)
            (Asns), mRNA.
ACCESSION   NM_013079
VERSION     NM_013079.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ono,A., Hirooka,K., Nakano,Y., Nitta,E., Nishiyama,A. and
            Tsujikawa,A.
  TITLE     Gene expression changes in the retina after systemic administration
            of aldosterone
  JOURNAL   Jpn J Ophthalmol 62 (4), 499-507 (2018)
   PUBMED   29713904
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Hester,S.D. and Nesnow,S.
  TITLE     Transcriptional responses in thyroid tissues from rats treated with
            a tumorigenic and a non-tumorigenic triazole conazole fungicide
  JOURNAL   Toxicol Appl Pharmacol 227 (3), 357-369 (2008)
   PUBMED   18164361
REFERENCE   3  (bases 1 to 2040)
  AUTHORS   Cui,H., Darmanin,S., Natsuisaka,M., Kondo,T., Asaka,M., Shindoh,M.,
            Higashino,F., Hamuro,J., Okada,F., Kobayashi,M., Nakagawa,K.,
            Koide,H. and Kobayashi,M.
  TITLE     Enhanced expression of asparagine synthetase under glucose-deprived
            conditions protects pancreatic cancer cells from apoptosis induced
            by glucose deprivation and cisplatin
  JOURNAL   Cancer Res 67 (7), 3345-3355 (2007)
   PUBMED   17409444
REFERENCE   4  (bases 1 to 2040)
  AUTHORS   Wang,Y., Maciejewski,B.S., Weissmann,G., Silbert,O., Han,H. and
            Sanchez-Esteban,J.
  TITLE     DNA microarray reveals novel genes induced by mechanical forces in
            fetal lung type II epithelial cells
  JOURNAL   Pediatr Res 60 (2), 118-124 (2006)
   PUBMED   16864689
REFERENCE   5  (bases 1 to 2040)
  AUTHORS   Ito,Y.N., Ito,M., Takita,H., Yoneya,S., Peyman,G.A., Gehlbach,P.L.
            and Mori,K.
  TITLE     Transpupillary thermotherapy-induced modification of angiogenesis-
            and coagulation-related gene expression in the rat posterior fundus
  JOURNAL   Mol Vis 12, 802-810 (2006)
   PUBMED   16885923
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2040)
  AUTHORS   Colletta,G. and Cirafici,A.M.
  TITLE     TSH is able to induce cell cycle-related gene expression in rat
            thyroid cell
  JOURNAL   Biochem Biophys Res Commun 183 (1), 265-272 (1992)
   PUBMED   1543496
REFERENCE   7  (bases 1 to 2040)
  AUTHORS   Greco,A., Gong,S.S., Ittmann,M. and Basilico,C.
  TITLE     Organization and expression of the cell cycle gene, ts11, that
            encodes asparagine synthetase
  JOURNAL   Mol Cell Biol 9 (6), 2350-2359 (1989)
   PUBMED   2569668
REFERENCE   8  (bases 1 to 2040)
  AUTHORS   Van Heeke,G. and Schuster,S.M.
  TITLE     Expression of human asparagine synthetase in Escherichia coli
  JOURNAL   J Biol Chem 264 (10), 5503-5509 (1989)
   PUBMED   2564390
REFERENCE   9  (bases 1 to 2040)
  AUTHORS   Cooney,D.A., King,V.D., Cable,R.G., Taylor,B. Jr. and Wodinsky,I.
  TITLE     L-Asparagine synthetase in serum as a marker for neoplasia
  JOURNAL   Cancer Res 36 (9 pt.1), 3238-3245 (1976)
   PUBMED   10081
REFERENCE   10 (bases 1 to 2040)
  AUTHORS   Huang,Y.Z. and Knox,E.W.
  TITLE     Glutamine-dependent asparagine synthetase in fetal, adult and
            neoplastic rat tissues
  JOURNAL   Enzyme 19 (5-6), 314-328 (1975)
   PUBMED   237754
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081719.1.
            
            On Jun 10, 2007 this sequence version replaced NM_013079.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081719.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q21"
     gene            1..2040
                     /gene="Asns"
                     /note="asparagine synthetase (glutamine-hydrolyzing)"
                     /db_xref="GeneID:25612"
                     /db_xref="RGD:2162"
     exon            1..156
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            157..428
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     CDS             180..1865
                     /gene="Asns"
                     /EC_number="6.3.5.4"
                     /note="glutamine-dependent asparagine synthetase"
                     /codon_start=1
                     /product="asparagine synthetase [glutamine-hydrolyzing]"
                     /protein_id="NP_037211.2"
                     /db_xref="GeneID:25612"
                     /db_xref="RGD:2162"
                     /translation="MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCC
                     FGFHRLAVVDPLFGMQPIRVRKYPYLWLCYNGEIYNHKALQQRFEFEYQTNVDGEIIL
                     HLYDKGGIEKTICMLDGVFAFILLDTANKKVFLGRDTYGVRPLFKALTEDGFLAVCSE
                     AKGLVSLKHSTTPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDEPLHAIYDS
                     VEKLFPGFEIETVKNNLRILFNNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKE
                     AQVPYALQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQSLDEVIFSLETY
                     DITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESER
                     LLKELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKDGIEKHLLRE
                     TFEDSNLLPKEILWRPKEAFSDGITSVKNSWFKILQDFVEHQVDDAMMSEASQKFPFN
                     TPQTKEGYYYRQIFEHHYPGRADWLTHYWMPKWINATDPSARTLTHYKSTAKA"
     exon            429..666
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            667..852
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            853..954
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1082
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1209
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1316
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1417
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1499
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1655
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..2016
                     /gene="Asns"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctgggcggcc ccagacagaa ggcaaaggag gccttcgtct agacaccatt tcttttactc
       61 tatgacatct aaacctggaa aacttcggcg ctccgagggt tttgcctggg accagctgga
      121 cgcctgcaca agaccagccg taattgggcg ccccagactg cctgcagccc tcctgtagca
      181 tgtgtggcat ctgggccctc ttcggcagcg atgactgcct ttccgtgcag tgtctgagtg
      241 cgatgaagat tgcgcacagg ggcccagatg cattccgttt tgagaacgtc aatggataca
      301 ccaactgctg ttttggcttc caccggctgg cggtggttga ccccctgttt ggaatgcagc
      361 caataagagt gaggaaatat ccttatctgt ggctgtgtta caacggtgaa atctacaacc
      421 acaaggcgct acaacaacgt ttcgaatttg agtatcagac caatgtggac ggtgagataa
      481 ttctccatct ctatgacaaa ggcggcatcg agaaaaccat ctgtatgttg gatggggtgt
      541 ttgcatttat cttactggac actgccaata agaaagtatt cctgggcaga gatacctatg
      601 gtgtcaggcc tttgtttaaa gccttgacag aagatggatt tctggctgtg tgttcagaag
      661 ccaaaggcct tgtctccttg aaacactcca ccaccccctt cctaaaagtg gagccctttc
      721 ttcctggaca ctatgaagtt ttggatttaa aaccaaatgg caaagtcgcg tctgtggaaa
      781 tggtcaaata ccatcactgt acggatgaac cactgcatgc catctatgac agtgtggaga
      841 aactcttccc aggctttgag atagagaccg tgaaaaacaa tctgcgtatc ctttttaaca
      901 acgctatcaa gaaacgcttg atgactgacc ggaggattgg ctgcctttta tcaggaggcc
      961 tggactccag cttggttgct gcctccctgc tgaagcaact caaggaggcc caagtgccct
     1021 atgctctcca gacatttgct atcggcatgg aagacagccc tgatctactg gctgccagaa
     1081 aggtggcaaa ttatattgga agtgagcatc atgaagtcct ttttaactct gaagaaggca
     1141 ttcagtccct ggacgaagtc atattttcct tggaaactta tgatattacg acagttcgag
     1201 catctgtagg tatgtattta atttccaagt atattcggaa gaacacagac agcgtggtga
     1261 tcttctccgg agaggggtca gatgagctta cacagggcta tatatatttc cacaaggcgc
     1321 cttctcctga gaaggcggag gaggagagtg agaggctcct gaaggaactc tacctgtttg
     1381 atgtcctccg tgccgaccgc actactgctg ctcacggtct cgaactgaga gtcccgtttc
     1441 tggatcatcg gttttcttcc tattacctgt ctctgccacc agaaatgaga attccaaaag
     1501 atggcataga aaaacatctc ctgagagaga cttttgagga ctccaacctg ctacccaaag
     1561 agattctctg gcgacccaag gaagccttca gtgatgggat cacctcagtc aagaactcct
     1621 ggttcaagat tttacaggac ttcgttgaac atcaggttga tgatgcgatg atgtctgagg
     1681 cctcccagaa atttcccttc aatactcccc aaactaaaga aggctattac taccgtcaga
     1741 tctttgaaca ccattacccc ggccgggctg attggctgac ccattattgg atgcccaagt
     1801 ggatcaatgc caccgaccct tctgcccgca ctctgaccca ttacaagtca actgccaaag
     1861 cttagacgct ctctacactc ttgtgtaaaa gtcaatgttt cttcttcctg ctctgaaggt
     1921 agagagacat tgaaacaatc agagagaatg aaagtcaacc atcagctgct caggcttatt
     1981 taggcatgga aagaaataaa agtatcacat ctaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
