LOCUS       NM_001107689           11393 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus ASH1 like histone lysine methyltransferase
            (Ash1l), mRNA.
ACCESSION   NM_001107689 XM_001074246 XM_227409
VERSION     NM_001107689.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Zeng,J., Gao,W., Tang,Y., Wang,Y., Liu,X., Yin,J., Su,X., Zhang,M.,
            Kang,E., Tian,Y., Ni,B. and He,W.
  TITLE     Hypoxia-sensitive cells trigger NK cell activation via the
            KLF4-ASH1L-ICAM-1 axis, contributing to impairment in the rat
            epididymis
  JOURNAL   Cell Rep 42 (11), 113442 (2023)
   PUBMED   37952156
  REMARK    GeneRIF: Hypoxia-sensitive cells trigger NK cell activation via the
            KLF4-ASH1L-ICAM-1 axis, contributing to impairment in the rat
            epididymis.
REFERENCE   2  (bases 1 to 11393)
  AUTHORS   Brinkmeier,M.L., Geister,K.A., Jones,M., Waqas,M., Maillard,I. and
            Camper,S.A.
  TITLE     The Histone Methyltransferase Gene Absent, Small, or Homeotic
            Discs-1 Like Is Required for Normal Hox Gene Expression and
            Fertility in Mice
  JOURNAL   Biol Reprod 93 (5), 121 (2015)
   PUBMED   26333994
REFERENCE   3  (bases 1 to 11393)
  AUTHORS   Eram,M.S., Kuznetsova,E., Li,F., Lima-Fernandes,E., Kennedy,S.,
            Chau,I., Arrowsmith,C.H., Schapira,M. and Vedadi,M.
  TITLE     Kinetic characterization of human histone H3 lysine 36
            methyltransferases, ASH1L and SETD2
  JOURNAL   Biochim Biophys Acta 1850 (9), 1842-1848 (2015)
   PUBMED   26002201
REFERENCE   4  (bases 1 to 11393)
  AUTHORS   Atta,H., El-Rehany,M., Hammam,O., Abdel-Ghany,H., Ramzy,M.,
            Roderfeld,M., Roeb,E., Al-Hendy,A., Raheim,S.A., Allam,H. and
            Marey,H.
  TITLE     Mutant MMP-9 and HGF gene transfer enhance resolution of
            CCl4-induced liver fibrosis in rats: role of ASH1 and EZH2
            methyltransferases repression
  JOURNAL   PLoS One 9 (11), e112384 (2014)
   PUBMED   25380300
  REMARK    GeneRIF: Data show that Ash1l and EZH2 methyltransferases protein
            expression was significantly elevated in fibrotic liver and
            decreased in mutant matrix metalloproteinase-9 (mMMP-9) and human
            hepatocyte growth factor (hHGF) gene transfer treated fibrotic
            livers.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11393)
  AUTHORS   Xia,M., Liu,J., Wu,X., Liu,S., Li,G., Han,C., Song,L., Li,Z.,
            Wang,Q., Wang,J., Xu,T. and Cao,X.
  TITLE     Histone methyltransferase Ash1l suppresses interleukin-6 production
            and inflammatory autoimmune diseases by inducing the
            ubiquitin-editing enzyme A20
  JOURNAL   Immunity 39 (3), 470-481 (2013)
   PUBMED   24012418
REFERENCE   6  (bases 1 to 11393)
  AUTHORS   Black,J.C., Van Rechem,C. and Whetstine,J.R.
  TITLE     Histone lysine methylation dynamics: establishment, regulation, and
            biological impact
  JOURNAL   Mol Cell 48 (4), 491-507 (2012)
   PUBMED   23200123
  REMARK    Review article
REFERENCE   7  (bases 1 to 11393)
  AUTHORS   Pinheiro,I., Margueron,R., Shukeir,N., Eisold,M., Fritzsch,C.,
            Richter,F.M., Mittler,G., Genoud,C., Goyama,S., Kurokawa,M.,
            Son,J., Reinberg,D., Lachner,M. and Jenuwein,T.
  TITLE     Prdm3 and Prdm16 are H3K9me1 methyltransferases required for
            mammalian heterochromatin integrity
  JOURNAL   Cell 150 (5), 948-960 (2012)
   PUBMED   22939622
REFERENCE   8  (bases 1 to 11393)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Nov 27, 2020 this sequence version replaced NM_001107689.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMEA5760383, SAMEA5760386
                              [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-158               JAXUCZ010000002.1  176644924-176645081
            159-674             JAXUCZ010000002.1  176660555-176661070
            675-5232            JAXUCZ010000002.1  176673101-176677658
            5233-5334           JAXUCZ010000002.1  176692567-176692668
            5335-6070           JAXUCZ010000002.1  176699845-176700580
            6071-6250           JAXUCZ010000002.1  176718374-176718553
            6251-6345           JAXUCZ010000002.1  176721779-176721873
            6346-6454           JAXUCZ010000002.1  176731239-176731347
            6455-6661           JAXUCZ010000002.1  176739836-176740042
            6662-6808           JAXUCZ010000002.1  176740213-176740359
            6809-6917           JAXUCZ010000002.1  176751726-176751834
            6918-7082           JAXUCZ010000002.1  176752920-176753084
            7083-7177           JAXUCZ010000002.1  176753250-176753344
            7178-7332           JAXUCZ010000002.1  176756350-176756504
            7333-7485           JAXUCZ010000002.1  176759995-176760147
            7486-7540           JAXUCZ010000002.1  176764273-176764327
            7541-7673           JAXUCZ010000002.1  176764408-176764540
            7674-7922           JAXUCZ010000002.1  176767792-176768040
            7923-8009           JAXUCZ010000002.1  176768868-176768954
            8010-8100           JAXUCZ010000002.1  176770463-176770553
            8101-8239           JAXUCZ010000002.1  176771812-176771950
            8240-8412           JAXUCZ010000002.1  176772069-176772241
            8413-8579           JAXUCZ010000002.1  176774485-176774651
            8580-8620           JAXUCZ010000002.1  176776415-176776455
            8621-8922           JAXUCZ010000002.1  176777508-176777809
            8923-11393          JAXUCZ010000002.1  176778377-176780847
FEATURES             Location/Qualifiers
     source          1..11393
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..11393
                     /gene="Ash1l"
                     /note="ASH1 like histone lysine methyltransferase"
                     /db_xref="GeneID:310638"
                     /db_xref="RGD:1306350"
     exon            1..158
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            159..674
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     CDS             258..9014
                     /gene="Ash1l"
                     /EC_number="2.1.1.354"
                     /note="probable histone-lysine N-methyltransferase ASH1L;
                     ash1 (absent, small, or homeotic)-like"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase ASH1L"
                     /protein_id="NP_001101159.1"
                     /db_xref="GeneID:310638"
                     /db_xref="RGD:1306350"
                     /translation="MDPRNTAMLGLGSDSEGFSRKSPSAISSGTLASKREVEIEGNTD
                     EEDPRKRNRERAIEAGKDDGLTDAQQQFSVKETNFSEGNLKLKIGLQAKRTKKPPKNL
                     ENYVCRPAIKTTVKHSRKALKSGKMTDEKNEHCPSKWDSSKLFKKAGDATAIECQSEE
                     SVHLHSQGENNPLSKKLSPVHSQMADYIPAAPPLVGSRDPDIKDRALLNGGTSVTEKL
                     AQLIATCPPSKSSKAKPKKLGTGTTVGLVSKDLIRKPGVGSIAGIIHKDLIKKPALST
                     AVGLVTKDPGKKPMFNAAVSLINKDSVKKLGTGTTAVFINKDLGKKPGTVTTVGLLSK
                     DPGKKLGIGIVPGLVNKESGKKLGLGTVVGLVNKELGKKLSSTVGLVAKDVTKKIVAS
                     SAMGLVNKDIGKKLLSCPIAGQLGSKDALNLKSEALLPTQEQLKASCSANISNHESQE
                     LPESLKDSATSKTFEKNVMRHSKESMLEKFSVRKEITNLEKEMFNEGTCIQQDSFSSN
                     ERGAFETSKHEKQPPVYCTSPDFQIGGASDASTAKSPFGAVGESNLPSSSPTVSVNPL
                     TRNPPETSSQLVPNPLLLNSTAEQMEEISESIGKNQFTAESTHLNVGHRSLGHSISIE
                     CKGIDKELNESKSTHLDISRISSSLGKKPSLASDSGIHTITPSVVNFTSLFSNKPFLK
                     LGAVAAPDKHCQVAESLSTSFQSKPLKKRKGRKARWTKVVARSTCRSPKGLDLERSEL
                     FKNVSCSSLSNSSEPAKFMKTIGASSFVDHDFLKRRLPKLSKSSAPSLALLADSEKAS
                     HKSFITHKLSSSMCVTSDLLSDIYKPKRGRPKSKEMPQLEGPPKRTLKIPASKVFSLQ
                     SKEEQEPPILQPEIEIPSFKQSLSVSPFPKKRGRPKRQMRSPVKMKPPVLSVAPFVAT
                     ESPSKLESESENHRSSSDFFESEDQLQDTDDLEDSHRQSVCSVSDLEMEPDKKISKRN
                     NGQLMKTIIRKINKMKTLKRKKLLNQILSSSVESSNKGKVQSKLHNTVSSLAATFGSK
                     LGQQINVSKKGTIYIGKRRGRKPKTVLNGLLSGSPASLAVLEQTAQQAAGSALGQILP
                     PLLPSPASSSEILPSPICSQSSGTSGGQSPVSSDAGFVEPSSVPYLHVHSRQGSMIQT
                     LAMKKAAKGRRRLSPPTLLPNSPSHLSELTSLKEATPSPVSESHSDETIPSDSGIGTD
                     NNSTSDRAEKFCGQKKRRHSFEHISLIPPETSTVLNSLKEKHKHKCKRRSHDYLSYDK
                     MKRQKRKRKKKYPQLRNRQDPDFIAELEELISRLSEIRITHRSHHFIPRDLLPTIFRI
                     NFNSFYTHPSFPLDPLHYIRKPDLKKKRGRPPKMREAMAEMPFMHSLSFPLSSTGFYP
                     SYGMPYSPSPLTAAPIGLGYYGRYPPTLYPPPPSPSFTTPLPPPSYMHAGHLLLNPTK
                     YHKKKHKLLRQEAFLTTSRTPLLSMSTYPSVPPEMAYGWMVEHKHRHRHKHREHRSEQ
                     PQVSMDTGSSRSVLESLKRYRFGKDTVGDRYKHKEKHRCHMSCPHLSPSKSLINREEQ
                     WVSREPSESSSLALGLQTPLQIDCSESSPSLSLGGFTPNSEPASSDEHMNLFTSAIGS
                     CRVSNPNSSCRKKLTDSPGLFPVQDTALSRPHRKEPLPSTERAIQSLAGSQSASDKPS
                     QRSSESTNCSPTRKRSSSESTSSTVNGIPSRSPRLVASLDDSVDCLLQRIVQHDEQES
                     VEKNGDAPITTVSAPPSSSPGHSYSKERTLGKSDSLLVPAVPSDSCNSIPLLSEKLAS
                     RCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFV
                     KRRPGRPRKCPLQAVVSMQAFQAAQFVNPELNEGEEVSLHLSPDTVTDVIEAVVQSVN
                     LNSEHKKGWKRKNWLLEEQTRKKQKTVPEEEEQENNKSFIEKPVEIPSPLETPAEPSE
                     PESNLQPVLALIPREKKAPRPPKKKYQRAGLYSDVYKTTDPKSRLIQLKKEKLEYTPG
                     EHEYGLFPAPIHVDVYVDVKPLSGYEATTCNCKKPDDDTRKGCGDDCLNRMIFAECSP
                     NTCPCGEQCCNQRIQRHEWVQCLERFRAEEKGWGIRTKEPLKAGQFIIEYLGEVVSEQ
                     EFRNRMIEQYHNHSDHYCLNLDSGMVIDSYRMGNEARFINHSCDPNCEMQKWSVNGVY
                     RIGLYALKDVPAGTELTYDYNFHSFNVEKQQLCKCGFEKCRGIIGGKSQRMNGLPSHK
                     GSQPASTHRKSARSKEKRKSKHKLKKRRGHPSEEPSENINTPTRLTPQLQMKPMSNRE
                     RNFVLKHHVFLVRNWEKIHQKQEEVKHSRDIHSTSLYTRWNGICRDDGNIKSDVFMTQ
                     FSALQTARSVRTRRLAAAEENLEVARAARLAQIFKEICDGIISYKDSSQQALAAPLLN
                     LPPKKKNADYYEKISDPLDLSTIEKQILTGYYKTVEAFDADMLKVFRNAEKYYGRKSP
                     IGRDVCRLRKAYYSARHEASAQIDEIVGETASEADSSETSVSEKENSHEKDDDVIRCI
                     CGLYKDEGLMIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQ
                     PGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIE
                     KLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLD
                     LYTYCKGRPKGVKEQDVYICDYRLDKSAHLFYKIHRNRYPVCTKPYAFDHFPKKLTPK
                     RDFSPHYVPDNYKRNGGRSSWKSERSKPLLKDLGQDDDALPLIEEVLASQEQAANEMP
                     SPEEPDQERVTGDVSDAEKKPEESSQEPQLASTPEERRHSQRERLNQILLNLLEKIPG
                     KNAIDVTYLLEEGSGRKLRRRTLFIPENSFRK"
     exon            675..5232
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            5233..5334
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            5335..6070
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            6071..6250
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            6251..6345
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            6346..6454
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            6455..6661
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            6662..6808
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            6809..6917
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            6918..7082
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            7083..7177
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            7178..7332
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            7333..7485
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            7486..7540
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            7541..7673
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            7674..7922
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            7923..8009
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            8010..8100
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            8101..8239
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            8240..8412
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            8413..8579
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            8580..8620
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            8621..8922
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
     exon            8923..11393
                     /gene="Ash1l"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctttggcttc tcctctttcg cgtttccgcc ggtccgctta cccgctggcc gcctcctgac
       61 aagcgggagg gttacggtgt ggacccagag gagccgaggc accaggctgc caccctctct
      121 ccccacttgc ctgctctcgg cgctctcggc ctcacacggg aaagagatgc tggttgcaag
      181 ttttggaaaa cactattttg tatggaagag tgttctgtaa aggttttgta agatcgctca
      241 gtaatagtgc acttgtgatg gaccctagga atactgctat gttaggattg ggttctgact
      301 ccgaagggtt ttcaagaaag agtccttctg ccatcagttc tggcactctg gccagcaaga
      361 gagaagtaga aatagagggt aacacagacg aagaggatcc tcgaaagcgg aatcgagaaa
      421 gggccataga agcaggaaaa gacgatggtt tgacagatgc acagcaacag ttttcagtga
      481 aagaaacaaa cttttcagag ggaaatttaa aattgaaaat tggccttcag gctaagagaa
      541 ctaaaaagcc tccaaagaac ttggagaact atgtgtgtcg ccctgccata aaaacaactg
      601 tcaagcactc aaggaaagca cttaaaagtg ggaagatgac agatgagaag aatgaacact
      661 gcccttcaaa atgggactct tcaaaattgt tcaagaaagc aggtgatgct acagccatcg
      721 aatgccaatc tgaagaaagt gtccatcttc actctcaggg ggaaaataac cctttgtcta
      781 agaagctttc tccagtgcat tcacaaatgg ccgattacat tcctgcagca ccacctcttg
      841 ttggcagtcg ggatcctgac ataaaggaca gagcattact taatggagga actagtgtaa
      901 cagaaaagtt ggcacagctc attgctacct gtcctccttc caagtcttcc aaggcaaaac
      961 cgaagaagtt aggaactggc actacagtag gattggttag caaggacttg atcaggaaac
     1021 caggtgttgg ttctatagct ggaataattc ataaagactt aattaaaaag cctgctctta
     1081 gcacagcagt tggattggta actaaagatc cagggaaaaa gcctatgttc aatgcagcag
     1141 taagcctgat caataaggac tctgtgaaaa aattgggaac tggcactaca gcggtattca
     1201 ttaataaaga cttaggcaaa aagccaggga ctgtcactac agtaggactg ctgagcaaag
     1261 acccagggaa gaagctagga attggtatcg ttccagggtt agtgaataaa gagtctggca
     1321 agaaattagg acttggcacc gtagttggac tggttaataa agagttggga aagaaattga
     1381 gttctacagt tggcctagtg gccaaggatg ttacgaagaa gattgtagca agctcagcaa
     1441 tgggattggt taataaggac attggaaaga aattattaag ttgccccatt gcaggacaac
     1501 tgggcagtaa agatgcctta aaccttaaat ctgaagcact gctccccact caggaacaac
     1561 ttaaggcttc ctgtagtgca aacataagta atcatgaaag tcaagaactt cctgagtctc
     1621 tgaaagacag tgctaccagc aaaacctttg agaagaatgt tatgcggcat agtaaagaaa
     1681 gcatgttgga aaagttttca gtccgaaaag aaattactaa tttggagaaa gaaatgttta
     1741 atgaaggaac atgcattcag caagacagtt tctcatctaa tgaaagggga gcctttgaga
     1801 cctcaaagca tgaaaagcaa cctcctgtgt attgcacttc tcctgacttt caaataggag
     1861 gtgcctctga cgcatctaca gctaaatctc cctttggtgc agtaggagaa agcaatcttc
     1921 cttcctcgtc acctactgta tctgttaacc ctttaaccag aaatccccct gaaacttctt
     1981 cacagttggt tccaaaccca ttacttttaa attctacagc agaacaaatg gaagaaattt
     2041 ctgaatctat tggaaagaac cagttcactg ctgaaagtac ccacttgaat gttggtcata
     2101 ggtcattagg tcatagcata agtattgagt gtaaaggaat tgataaagaa ctaaatgaat
     2161 caaaaagtac acatctagat atttctagaa taagttcttc cttgggaaaa aagccaagtt
     2221 tggcttctga ctctggtatt catacaatta ctccttccgt tgttaatttt actagtttat
     2281 ttagtaacaa accttttcta aagcttggtg cagtagctgc accagacaag cactgccagg
     2341 tcgctgaaag cctaagcact agtttccagt ccaaaccatt gaaaaaaaga aaagggagaa
     2401 aagctcggtg gactaaagtg gtggcaagaa gcacatgccg atccccaaaa ggactagact
     2461 tagaacggtc agagcttttt aagaatgttt cctgtagctc actatcaaat agttctgagc
     2521 cagcaaagtt tatgaaaact atcggagcat cgtcttttgt cgatcatgac ttccttaaac
     2581 gccgattgcc aaagttgagt aagtcttcag ctccatctct tgctctttta gccgacagtg
     2641 agaaagcatc tcataagtct tttatcactc acaaactgtc ctccagtatg tgtgtcacta
     2701 gtgatctttt gtcggatatt tataagccca aaagaggaag acccaaatcc aaggaaatgc
     2761 ctcaactaga aggtccacct aaaaggactt taaaaatacc tgcctctaaa gttttttctt
     2821 tacagtctaa agaagaacaa gaacccccaa ttttacaacc agaaattgaa attccttcct
     2881 tcaaacaaag tttgtctgta tctccttttc caaaaaagag aggcaggcct aagaggcaga
     2941 tgaggtctcc agtgaagatg aagccccctg tactgtcagt ggctccattt gttgccactg
     3001 aaagtccaag taagctagag tctgaaagtg agaaccatag aagtagcagt gatttctttg
     3061 agagtgagga tcagcttcag gatacagatg acttagagga cagtcatagg caaagtgtct
     3121 gcagtgtgag tgacctggag atggagccgg ataaaaaaat tagcaagcga aacaatggac
     3181 aattaatgaa aacaattatc cgcaaaataa ataaaatgaa gactttaaag agaaaaaaat
     3241 tgttgaatca gattctttca agttctgtag aatcaagtaa taaaggaaaa gtacagtcca
     3301 aacttcataa tacagtatca agtcttgctg ccacgtttgg ctctaaattg gggcaacaga
     3361 taaatgtcag caagaaagga accatttaca tagggaagag aagggggcga aaaccaaaaa
     3421 ctgtcttaaa tggccttctt tctggtagcc ctgccagcct tgctgtgctt gaacaaacgg
     3481 ctcagcaggc agctgggtcc gcattaggac agatcctccc cccgttactg ccttcacctg
     3541 ctagtagctc tgagatcctt ccatcaccta tttgctctca gtcttctggg actagtggag
     3601 gtcagagccc tgtcagtagt gacgcaggct ttgttgaacc tagttcagtg ccatatttgc
     3661 atgtgcactc aagacagggc agtatgattc agactcttgc aatgaagaag gctgccaaag
     3721 gcaggaggcg gctatctcct cctactttgt tgccaaattc tccttctcat ttgagtgaac
     3781 tcacatccct gaaagaagcc actccttccc cagttagtga gtctcatagt gatgagacca
     3841 ttcccagtga cagtggcatt gggacagata ataatagcac atcagacagg gcagagaagt
     3901 tttgtggaca gaaaaaaagg agacattctt ttgagcacat ttctctgatt ccccctgaaa
     3961 cttctaccgt cctaaacagt cttaaagaga aacacaaaca caaatgtaag cgcaggagtc
     4021 acgattacct cagctatgac aagatgaaaa gacaaaagcg aaaacggaaa aagaaatatc
     4081 cccagcttcg aaataggcag gatccagact tcatcgcaga actagaggag ctgataagtc
     4141 gtctaagtga gattcggatt acccatcgaa gtcatcattt cattccccga gacctcctgc
     4201 caactatttt tcgaatcaac tttaatagtt tctatacaca tccttctttt cccttagacc
     4261 ctttgcacta cattcggaag cctgacttaa aaaagaaaag agggaggccc cctaagatga
     4321 gggaggcaat ggcagaaatg ccctttatgc acagccttag ttttccttta tctagtactg
     4381 gattttaccc ttcttatggc atgccttatt ctccttcacc cctcactgct gctccgatag
     4441 gattagggta ttatggacgg tatcccccca ctctctatcc accacctcca tctccttcat
     4501 tcaccactcc actgccacct ccctcctata tgcatgctgg tcatttattg ctcaatccta
     4561 ccaaatatca taagaaaaag cataagctcc ttagacagga ggcttttctt acaaccagca
     4621 ggactcctct cctttccatg agcacctacc ccagcgtacc tcctgagatg gcttatggtt
     4681 ggatggtgga gcacaaacac agacaccgtc acaaacacag agagcaccgt tctgaacagc
     4741 cccaggtttc catggatact ggctcctcca gatcggtctt ggagtctttg aaacgttacc
     4801 gatttggtaa ggatactgtt ggagatcgct ataagcataa ggaaaagcac aggtgtcaca
     4861 tgtcctgccc tcatctctct ccttcaaaga gcttaataaa cagagaagaa cagtgggttt
     4921 ctcgagagcc ttcagaatca agttctttag ccttgggatt gcaaacacct ttacaaattg
     4981 actgctcgga aagttctcca agtttgtccc ttggaggatt tactcccaat tctgagccag
     5041 ccagcagtga tgaacatatg aaccttttta caagtgcaat aggcagctgc agagtttcaa
     5101 accctaactc gagctgccgg aagaagttaa ctgacagccc tggactcttt cctgtacaag
     5161 atactgcact aagtcggcct cacagaaagg aaccgttgcc ttccactgaa agggcaatac
     5221 aatcgttggc aggttcccag tcagcttccg ataaaccttc ccagcgatca tcagaaagca
     5281 caaattgtag ccctacccgt aaaaggtctt catctgaaag tacttcttca acagtgaatg
     5341 gaattccttc cagaagtccc agattggttg cttctctgga tgactctgta gactgtctgt
     5401 tgcagcggat agttcaacat gatgagcaag agtccgtgga gaaaaatggt gatgctccaa
     5461 ttacaactgt atctgcacca ccttcttcca gtccaggcca tagctacagc aaagagcgga
     5521 ccctagggaa atcagacagt cttctagtgc ctgcagtccc gagtgactct tgtaatagta
     5581 tcccacttct ttctgaaaag ttagcaagca gatgttcccc tcatcatatc aagagaagtg
     5641 tagttgaagc tatgcagcgc caagctcgga aaatgtgcaa ttatgacaaa atcttggcta
     5701 ccaagaaaaa tctggaccat gtcaataaaa ttttaaaagc caaaaaactt caaaggcagg
     5761 ccaggacagg aaacaatttt gtgaaacgca gaccaggtcg acctcggaaa tgtcccctcc
     5821 aagctgtggt gtcaatgcaa gcattccagg ctgcccagtt tgtcaaccca gaattgaacg
     5881 aaggcgaaga agtgtcgctg cacctaagtc cagacacagt tacggatgtg atcgaggctg
     5941 tggttcagag tgtgaacctt aattcagaac ataagaaggg gtggaagagg aaaaattggc
     6001 tgttagaaga acagactaga aaaaagcaga agacagtacc agaggaagaa gaacaagaaa
     6061 acaataaaag ctttatagaa aaaccagttg aaattcccag tcctcttgaa accccggctg
     6121 aaccttctga acctgaaagt aacttacaac ctgtgctggc tctcatccca cgagaaaaga
     6181 aagccccacg tcctccaaag aagaaatacc agagagctgg gctgtattct gatgtctaca
     6241 aaactacaga cccaaagagc cgattaatcc aactgaagaa agagaagttg gagtacactc
     6301 cgggagagca tgagtatgga ctattcccag cacccattca tgttgatgtc tacgttgatg
     6361 tcaaacctct ttctggttat gaggctacca cctgtaattg taagaagcca gatgatgaca
     6421 ccaggaaggg ctgtggggat gactgcctca atagaatgat ctttgctgag tgttccccta
     6481 acacgtgccc ctgtggtgag cagtgctgta accaaaggat acagagacat gagtgggtgc
     6541 agtgtctgga acgatttcga gctgaggaaa aaggttgggg aattagaacc aaagagcccc
     6601 taaaagctgg ccaattcatc attgaatacc tgggggaagt tgtcagcgaa caagaattca
     6661 ggaacaggat gattgaacag tatcataatc acagtgacca ctactgttta aacctggata
     6721 gtggaatggt gattgacagt taccgcatgg gaaacgaggc cagattcatc aaccacagct
     6781 gtgacccaaa ttgtgaaatg cagaaatggt ctgttaacgg agtgtaccgc attggattgt
     6841 atgcacttaa ggatgtaccc gctgggaccg agctcaccta cgattacaac tttcattcct
     6901 tcaatgtaga aaaacagcaa ctttgtaaat gtggttttga gaagtgtcga ggaatcattg
     6961 gaggcaaaag tcaacgaatg aatggactcc ctagccataa aggcagccag cctgcaagca
     7021 cacatagaaa gtctgcacgg tcgaaagaaa agcggaagtc caagcacaag ctgaagaaaa
     7081 ggagaggcca tccctctgaa gagcccagtg aaaacatcaa caccccaaca agattgactc
     7141 cacagttaca gatgaagcca atgtccaata gagagaggaa ctttgtgtta aagcatcatg
     7201 tgttcttggt ccgaaactgg gagaagatcc atcagaagca ggaggaagta aagcacagcc
     7261 gtgacatcca ctccacatcc ctgtacaccc gatggaatgg catctgtaga gacgatggga
     7321 acattaagtc tgatgtcttc atgacccagt tctctgccct gcagacagcg agatctgttc
     7381 gcacaagacg cttggcagct gcagaggaga acctggaagt ggctcgggca gcacgcctgg
     7441 cccagatctt caaagagatc tgcgatggca tcatctccta caaagattct tcccaacaag
     7501 ctcttgcagc tccactgttg aatcttcccc caaagaaaaa aaatgctgac tattatgaga
     7561 agatctctga cccgctggat ctcagcacca tagagaagca gatcctcact ggatactaca
     7621 aaaccgtgga agcttttgat gccgacatgc ttaaggtctt tcgaaatgct gagaagtatt
     7681 atgggcgtaa atccccgatt gggagagatg tttgccgctt aagaaaggcc tattacagtg
     7741 cccggcatga agcttcagct caaatcgatg agattgtggg agaaacagcc agtgaggcag
     7801 acagcagtga gacctcagtg tctgagaagg aaaacagcca tgagaaggac gatgatgtga
     7861 tccgctgcat ctgcggcctc tacaaggatg aaggcctcat gatccaatgt gacaagtgca
     7921 tggtgtggca gcactgtgac tgcatgggtg tgaacacaga cgtggagcac tacctttgtg
     7981 agcagtgtga cccccggccc gtggacaggg aggtacccat gatccctaga ccccactatg
     8041 cccagcctgg gtgtgtctac tttatctgtt tactccgaga tgatttgctg cttcgtcagg
     8101 gtgattgtgt gtacctgatg agggacagtc ggcgcacacc tgatggccac ccagtccgtc
     8161 agtcctatag actgttatct cacattaacc gagataaact tgacatcttt agaatcgaga
     8221 agctttggaa gaatgaaaaa gaagaacggt ttgcctttgg tcaccattat ttccgccccc
     8281 atgaaactca ccactctcca tcccgtcggt tctatcacaa tgagctattt cgggtgccac
     8341 tctatgagat cattcccttg gaagctgtag ttgggacctg ctgtgtgttg gacctttata
     8401 cgtactgtaa agggagaccc aaaggagtaa aggaacaaga tgtatacatc tgtgattatc
     8461 ggcttgacaa gtcagcacac cttttttaca agatccatcg gaatcgctat cctgtctgca
     8521 ccaaacccta tgcctttgat catttcccca agaagctcac tcccaaaaga gacttctcac
     8581 ctcattatgt cccagataac tacaagagga acggaggacg atcgtcctgg aagtctgaac
     8641 gttcaaagcc gctcttaaaa gacctcggac aagatgatga tgctttgccc ttgattgaag
     8701 aggtgctggc tagtcaggag caagcagcca atgaaatgcc cagccctgag gagccagacc
     8761 aggaaagagt cactggggac gtcagtgacg ctgaaaagaa gccagaggag agtagtcaag
     8821 agccccagtt agccagcacg cctgaggagc gaaggcacag ccagagggaa cggctcaacc
     8881 agatcctgct caacctcctg gagaaaatac ctgggaaaaa cgccattgat gtgacctacc
     8941 tgctggagga agggtcaggc aggaaactac gaaggcgcac tttgtttata ccagaaaaca
     9001 gctttcggaa gtgactctca aaaggatgag aacctcgagc atctgggatc cagcggcacc
     9061 gtggagtcca gtctcttgct ctctggacat ggactggggc aaaaaagttc tgtccaggca
     9121 aggaatgtga ggtcacgtgt ctgacattag gtacttatta gacttggagc tagtgaaaag
     9181 ggagtggaag taggcttgtc tgagacggtt gagtttactc ctttgtagct ttgcctaagg
     9241 gttaatatgg agaggaggac cacattggaa tagccagaag ctattggtac tttacagcac
     9301 ttgatactcc tcacagcttg ggtttttctg ggttgccctc tgatcctgca agcactaaga
     9361 agctgcttcc tatgaccagg tgtaaattca aaatccaaag ggaagggtcc agaccccagc
     9421 cctatcccat ttattctctc agctctaaga gctgcatcag agacctacac agagacagta
     9481 gctgaggcct cttttcagtt ccttcaacag gaaagttctt tcttccttct tgcctaacca
     9541 ggtcaaagta ctagaacagg aatcaactgc tcagagcact tagaacttcc gccactctcc
     9601 actctctttc ccaggagaga gttgatagag tcagggggag gagaggcccc atgggatcag
     9661 gagggcagta tttgtaatta tttttggttt acccttcata acctgccgaa catatttttt
     9721 cctactgaga aagccaagcc cccgccagca cacatgctgc ttctcttcct gtgtgtctgc
     9781 tgtgcaccga ttcacttcac aaaagaacaa catttttaaa cttctcccct aacaaaactc
     9841 actacagaac cgttgagcag tgttcactat cagagtgttt gttctaagtg tagattaccg
     9901 attgaaaaca ctattcttat tttcttaact gtatagttcc cgtgtccatc cctgcactca
     9961 tcccgtttcc cctccacctt tctgaatgac ttcatcatca ggaggtatat ccagggttgc
    10021 cgtccctccc agtctcttgg ccggaagtta ccagaccata ctgtctcttt acaagtaaaa
    10081 ttcaaaaagc tttgctttgt cttctcagac atacatatgc atatacattt tagatgttct
    10141 tataagagaa aagatggttt ttaaatgtgc caagttatgt atgtgtgtgt atatatatgt
    10201 gtgtgtgtgt gtatatatat atgtgtgtct gtgtatatat gtgtgggtgc gtgtgagtat
    10261 gtgtgtgtgt gggcacacgc acatgtgcac aagcgttttg tgtgtgtgtg tgtgtgtgtg
    10321 tgtgtgtgtg tgtgtgtgtg tgctgtgaga ttggtaagca atacattagt aaacacatct
    10381 tcattactct tttccgatac tggaccaatg atgtcctaac tgtacatttc cttcccctta
    10441 tgatgacgac gctctgactc atttaggtag aaacatttga ccacctttca ttccattgat
    10501 tttctttttc tcctttctgt gttattctta agggaaaaga gagagagaaa gagaggggga
    10561 tagcatagat ccccttgagc agagaaaaag caaaatattt tattaaagaa aaaaagagaa
    10621 attaagaaaa tagtttggag tattttctta ctgtagagaa gcattgtaca ttaccacgag
    10681 acctgggtat aagatgctca cgtgtggcgc tggggagcgc atgtccaagc ccatttgagt
    10741 ggtttctttt gtttctcatt gcagggagtg ggtgggaggg agggaggact aagggcactt
    10801 tcgggggtct ccttttagtc aaaagcaaga aaatgacaag aaagagatta aattcaatgt
    10861 tttccttgaa tagtgttaaa cactaaaatt ttaaaacaag aaagaaaaaa ctttgtaaaa
    10921 tgcaagaaca gaagcaaaag acactacgct gtcattttta tctttctttt gttgaaagat
    10981 taaaaactga aatgtttttt agacaatcaa atgttaggta agtgcaaaaa cttgtttttc
    11041 ttactggtgt agaaattaat gcctttttta tttttcagtt attttataat aacaaaataa
    11101 aaacaacccc cagctgccag gtgggttttg gtgttcgaaa tgcggggcaa agcactacat
    11161 cactgcaaat agatacagag ttagtctgca tgtctgtagg ctgtgtgatt gcggaaaata
    11221 taaatgctgc taatatattt cctttttaca gaaaagcata tctaaataga tgattgtttt
    11281 gatgttaatc tttgtaaatt atgtattacc aattttaaca ttggatgtaa ttgcataaag
    11341 gcttgcatct caatccttga aagtctagca ttaaatggaa acaacttttc cta
//
