LOCUS       NM_053646               4914 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus N-acylsphingosine amidohydrolase 2 (Asah2), mRNA.
ACCESSION   NM_053646
VERSION     NM_053646.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Xu,Y.N., Wang,Z., Zhang,S.K., Xu,J.R., Pan,Z.X., Wei,X., Wen,H.H.,
            Luo,Y.S., Guo,M.J. and Zhu,Q.
  TITLE     Low-grade elevation of palmitate and lipopolysaccharide
            synergistically induced beta-cell damage via inhibition of neutral
            ceramidase
  JOURNAL   Mol Cell Endocrinol 539, 111473 (2022)
   PUBMED   34610358
  REMARK    GeneRIF: Low-grade elevation of palmitate and lipopolysaccharide
            synergistically induced beta-cell damage via inhibition of neutral
            ceramidase.
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Sakamoto,W., Coant,N., Canals,D., Obeid,L.M. and Hannun,Y.A.
  TITLE     Functions of neutral ceramidase in the Golgi apparatus
  JOURNAL   J Lipid Res 59 (11), 2116-2125 (2018)
   PUBMED   30154232
REFERENCE   3  (bases 1 to 4914)
  AUTHORS   Airola,M.V., Allen,W.J., Pulkoski-Gross,M.J., Obeid,L.M.,
            Rizzo,R.C. and Hannun,Y.A.
  TITLE     Structural Basis for Ceramide Recognition and Hydrolysis by Human
            Neutral Ceramidase
  JOURNAL   Structure 23 (8), 1482-1491 (2015)
   PUBMED   26190575
REFERENCE   4  (bases 1 to 4914)
  AUTHORS   Zhu,Q., Kang,J., Miao,H., Feng,Y., Xiao,L., Hu,Z., Liao,D.F.,
            Huang,Y., Jin,J. and He,S.
  TITLE     Low-dose cytokine-induced neutral ceramidase secretion from INS-1
            cells via exosomes and its anti-apoptotic effect
  JOURNAL   FEBS J 281 (12), 2861-2870 (2014)
   PUBMED   24798654
REFERENCE   5  (bases 1 to 4914)
  AUTHORS   Novgorodov,S.A., Wu,B.X., Gudz,T.I., Bielawski,J.,
            Ovchinnikova,T.V., Hannun,Y.A. and Obeid,L.M.
  TITLE     Novel pathway of ceramide production in mitochondria: thioesterase
            and neutral ceramidase produce ceramide from sphingosine and
            acyl-CoA
  JOURNAL   J Biol Chem 286 (28), 25352-25362 (2011)
   PUBMED   21613224
  REMARK    GeneRIF: Novel pathway of ceramide production in mitochondria:
            thioesterase and neutral ceramidase produce ceramide from
            sphingosine and acyl-CoA.
REFERENCE   6  (bases 1 to 4914)
  AUTHORS   El Bawab,S., Birbes,H., Roddy,P., Szulc,Z.M., Bielawska,A. and
            Hannun,Y.A.
  TITLE     Biochemical characterization of the reverse activity of rat brain
            ceramidase. A CoA-independent and fumonisin B1-insensitive ceramide
            synthase
  JOURNAL   J Biol Chem 276 (20), 16758-16766 (2001)
   PUBMED   11278489
REFERENCE   7  (bases 1 to 4914)
  AUTHORS   El Bawab,S., Roddy,P., Qian,T., Bielawska,A., Lemasters,J.J. and
            Hannun,Y.A.
  TITLE     Molecular cloning and characterization of a human mitochondrial
            ceramidase
  JOURNAL   J Biol Chem 275 (28), 21508-21513 (2000)
   PUBMED   10781606
REFERENCE   8  (bases 1 to 4914)
  AUTHORS   Tani,M., Okino,N., Mori,K., Tanigawa,T., Izu,H. and Ito,M.
  TITLE     Molecular cloning of the full-length cDNA encoding mouse neutral
            ceramidase. A novel but highly conserved gene family of
            neutral/alkaline ceramidases
  JOURNAL   J Biol Chem 275 (15), 11229-11234 (2000)
   PUBMED   10753931
REFERENCE   9  (bases 1 to 4914)
  AUTHORS   Tani,M., Okino,N., Mitsutake,S., Tanigawa,T., Izu,H. and Ito,M.
  TITLE     Purification and characterization of a neutral ceramidase from
            mouse liver. A single protein catalyzes the reversible reaction in
            which ceramide is both hydrolyzed and synthesized
  JOURNAL   J Biol Chem 275 (5), 3462-3468 (2000)
   PUBMED   10652340
REFERENCE   10 (bases 1 to 4914)
  AUTHORS   El Bawab,S., Bielawska,A. and Hannun,Y.A.
  TITLE     Purification and characterization of a membrane-bound nonlysosomal
            ceramidase from rat brain
  JOURNAL   J Biol Chem 274 (39), 27948-27955 (1999)
   PUBMED   10488143
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1 and AB057433.1.
            
            On Mar 4, 2015 this sequence version replaced NM_053646.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript from the same
            strain was available for the full length of the gene. The extent of
            this transcript is supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB057433.1, SRR26643298.7743.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                JAXUCZ010000001.1  239352442-239352493 c
            53-2491             AB057433.1         1-2439
            2492-4914           JAXUCZ010000001.1  239278994-239281416 c
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q52"
     gene            1..4914
                     /gene="Asah2"
                     /note="N-acylsphingosine amidohydrolase 2"
                     /db_xref="GeneID:114104"
                     /db_xref="RGD:69410"
     exon            1..116
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            117..248
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..2407
                     /gene="Asah2"
                     /EC_number="3.5.1.23"
                     /note="NCDase; acylsphingosine deacylase 2;
                     neutral/alkaline ceramidase; N-acylsphingosine
                     amidohydrolase (non-lysosomal ceramidase) 2"
                     /codon_start=1
                     /product="neutral ceramidase"
                     /protein_id="NP_446098.1"
                     /db_xref="GeneID:114104"
                     /db_xref="RGD:69410"
                     /translation="MAKRTFSSLEAFLIFLLVMMTAITVALLTLLFVTSGTIENHKDS
                     GNHWVSTTQGPTTTQSSPTTQTPTTQTPDLPPSQNFSGYYIGVGRADCTGQVSDINLM
                     GYGKNGQNAQGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLQSKY
                     GSLYRRDNVILSATHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIVKSIDIAHTN
                     LKPGKVLINKGNVANVQINRSPSSYLQNPPSERARYSSDTDKEMVVLKLVDLNGEDLG
                     LISWFAVHPVSMNNSNHLVNSDNMGYAAYLFEQEKNRGYLPGQGPFVAGFASSNLGDV
                     SPNILGPHCVNTGESCDNDKSTCPSGGPSMCMASGPGQDMFESTHIIGRVIYQKAKEL
                     HASASQEVTGPVLTAHQWVNMTDVSVQLNATHTVKTCKAALGYSFAAGTIDGVSGLNI
                     TQGTTEGNLFWDTLRDQLLGKPSEEIIECQKPKPILIHTGELTKPHPWQPDIVDIQIV
                     TLGSLAIAAIPGEFTTMSGRRLREAVKKEFALYGMKDMTVVIAGLSNVYTHYITTYEE
                     YQAQRYEAASTIYGPHTLSAYIQLFRALAKAIATDTVANMSSGPEPPFFKNLIGSLIP
                     NIADRAPIGKQFGDVLQPAKPEYRVGEVVEVVFVGANPKNSAENQTHQTFLTVEKYED
                     SVANWQIMHNDASWETRFYWHKGVLGLSNATIHWHIPDTALPGVYRIRYFGHNRKQEL
                     LKPAVILAFEGISSPFEIVTT"
     exon            249..424
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            425..574
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            575..751
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            752..879
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            880..957
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1078
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1204
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1291
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1396
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1478
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1594
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1689
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1690..1729
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1825
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1921
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2068
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2128
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2217
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..4914
                     /gene="Asah2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atttggaaac acattgagaa gtcagcctgg gcaacctgca gggcacaagg cacaggattc
       61 tgcatggcca tttggactcc cgggaggtga ggacttgctt tgattcacag agtcaggaga
      121 aatggcaaag cgaaccttct cctccttgga ggcgttcctc attttccttc tggtaatgat
      181 gacggccatc acagtggccc ttctcaccct cttgttcgtc accagtggga ccatcgaaaa
      241 ccacaaagat tcagggaatc actgggtttc aaccacccag ggccccacaa ccacccagtc
      301 ctctccaacc acacaaactc caaccacaca aactccagac ctccctccct ctcagaactt
      361 cagtggctac tacattggtg ttgggcgagc tgactgcacc ggacaagtgt cagatatcaa
      421 tttgatgggc tatggcaaaa atggccagaa cgcacagggt ctcctcacca ggctgttcag
      481 ccgcgctttt atcttggcgg atccagatgg gtcaaatcga atggcatttg tgagcgtgga
      541 actatgcatg atttcccaac gactcaggtt ggaggtcttg aagagactac agagtaaata
      601 tggctctctg tatcgaagag acaacgttat cctgagtgcc actcacactc actctggccc
      661 agcaggattt ttccaatata cactctatat acttgccagc gagggattca gcaaccggac
      721 ctttcagtac atagtctctg ggatcgtgaa gagcattgat atagcacaca caaatcttaa
      781 accgggcaaa gtccttatca acaaaggaaa tgttgctaat gtgcagatca accgcagtcc
      841 ctcctcttac cttcagaatc caccttcgga gagagcaagg tattcttccg acacggacaa
      901 ggaaatggtc gtcttgaaac tggtggattt gaatggagaa gacttgggcc ttatcagctg
      961 gtttgccgtc caccccgtga gcatgaacaa cagtaaccac ctcgtcaaca gtgacaatat
     1021 gggctacgcg gcttaccttt tcgagcaaga aaagaacaga ggctatctgc ctggacaggg
     1081 accattcgta gcaggctttg cttcatcaaa tctcggagac gtgtcgccca acattcttgg
     1141 cccacattgt gtcaacacag gggagtcttg tgacaacgac aaaagcacct gtcccagtgg
     1201 tgggcctagc atgtgcatgg ccagtggacc cggacaagat atgtttgaga gcacacacat
     1261 tataggacgg gtcatctatc agaaagccaa ggagctgcat gcctctgcct cccaggaagt
     1321 gaccggccca gtgctcacag ctcaccagtg ggtgaacatg acggatgtga gcgtccaact
     1381 caatgccaca cacacagtga agacgtgtaa agccgccctg ggctacagtt ttgccgcagg
     1441 cacaattgat ggagtttcgg gcctcaatat tacacaggga acaacagaag ggaatctatt
     1501 ttgggacact cttcgggacc agctcttggg aaaaccatct gaagaaatca tagaatgcca
     1561 gaaacctaaa ccaatcctaa tccacacggg agagctgaca aaacctcatc cttggcaacc
     1621 agatattgtt gatattcaga tcgttactct tggttcgttg gccatagctg ctatccctgg
     1681 ggaatttaca accatgtcag gtcgaagact tcgtgaggca gttaaaaaag aatttgcact
     1741 ttatgggatg aaggatatga ctgtcgttat cgcagggctg agcaatgttt atacccatta
     1801 cattaccaca tatgaagaat accaggctca gcggtatgag gcagcatcta cgatttatgg
     1861 accacacacc ctgtctgcgt acatccagct cttcagagcc cttgctaagg ccattgctac
     1921 ggatacagta gccaacatga gcagcggtcc tgagcctcca ttcttcaaaa acctgatagg
     1981 ttcacttatt cctaatattg cggatagagc accaataggc aaacaatttg gggatgtcct
     2041 gcagccagca aaacctgaat acagagtggg agaagtggtt gaagttgtct ttgtaggcgc
     2101 taacccaaag aattcagcag aaaaccagac ccatcaaacc ttcctcactg tggagaaata
     2161 cgaggactct gtagccaact ggcagataat gcataacgat gcctcctggg agacgagatt
     2221 ttattggcac aagggagtac tgggtctgag caatgcaaca atacattggc atattccaga
     2281 tactgccctc cctggagtct acagaatcag atattttgga cacaatcgga agcaggaact
     2341 tctaaagcct gctgtcatac tagcatttga aggaatttct tccccttttg aaattgtcac
     2401 tacttagtga aaagttgaca gatcttgaag aacagctttt ctctgtgcac attatagagt
     2461 gatttcacac aaatgtgaac taccagttta atttttgtaa ttgtctctgt ttgagggaca
     2521 gattgtttat tgtgaatgga cagaggtatg tgtttgtgtt gttgtgtgat taggtgcatg
     2581 catggtaaca gggagagaga atgagagaga gagagagaga gtgagagtgt gcacgcaagc
     2641 gaccgagcga gaggcagaga taaagagagt tatcagtgag caagagaata ttagagaaga
     2701 gccactggta accataagcc ttgttatctt tatctaaagc atgattttcc ttgaagcttt
     2761 gtggttgttt aagagataat tccctctaat atgaaatccc tgatgtgtag tgatagtatg
     2821 tgaagatatg tgaatgatgt ttatgctatt tatttataga cttattaaat gagaatacta
     2881 gagagctttc tagaagtcct ctagaatgat acttgatttt acagagagga aaaaggaggt
     2941 ttacttctca ttaggttaga ataaggttac tatatatttt tccttggtta tagttacaaa
     3001 ataacatgta actttaccac aaatatttga gcccagctaa aatataccca ggaaattagc
     3061 atgccaggtt ttggtttgtt tttgcattca aacaagcata gtccttctga tcactccctt
     3121 tagaatggat ctgcctggct cagggtcatt gctcatgctc agatcatttc tggaattgcc
     3181 tcccaagtcc aactatgcga atgtcacttg cagtgctttg actatgcctc gtattcctca
     3241 aagtgtcctt atcctgctaa gtaatgtctc ttcttcccca aatttactct aagtgatttt
     3301 aaaatgtttt caccaatcaa acgtacctca cactacaaag ctttgccttg aatttagatt
     3361 ttgaaacaaa agtatgaagg ctggaaatgt agtcatcaag ggggaagttt taaatgtatc
     3421 tgtctgttct ttcatcagct actcactccc tcatggggat cactgaatag ttatttaaaa
     3481 ccacatgtcc aatgttgtac cattcctggg tcttcacaat tacagacatc atcataatga
     3541 gatgattggg tagatgattc ctttgaaggg taaaatattc aaccagacat ataaattctg
     3601 aagagacatt tgaaaactat aatgctcatg tctctatttt gtgtcttctg ggctgggtat
     3661 tctcatttgg gaggaaagag ggcccaaaca atttttcaat attaatctga aagtaatgtt
     3721 ttagaaatta tgatctagac agtctaatga atgtgatggg gcaatgtagc atgaattgtc
     3781 tgaaagtacg atttaaaatt acttagatta actaatgaag tcaatatttg aacaccaatc
     3841 agacttttgt agataggtaa aaagaaagca ttttaggaga aaccgatctt tatttcaaaa
     3901 ctaaatttgt cttataaaca atagtggtaa ggtatacctt cacttatttt ccctatggtt
     3961 tacctactta aacactgtag attgactcaa aaggcatctg gagagttttt tggtgactcc
     4021 atttcccact tggcatttct atgggtatta actgctaaag aataaactga tctggatttt
     4081 caagggtggt gaacatgtct cctgatggga gggaacaccc tattatgttc aaggttcaaa
     4141 atagttgatc tcagaacaaa aacaagaaca agaacaagaa caacacacac acacacacac
     4201 acacacacac acacacacac acactcccat ggtattattt aaaatttatt tctcatgact
     4261 cagaacaata caagggttta tttcccatca ccattctctc cagtgaagct tttttcaaag
     4321 tctacatagg agtttctcct ttctcactga taactatgcc acagtggcta ctacatcact
     4381 agtaatttac atgcccagcc ctgcatggaa ataaatcaca gaaacataat ttcccagtag
     4441 acttagtctc ttccagcctg tgtacttcta gtgtttgcaa tctgtaaaaa accaactagg
     4501 tccctgcatt atccaaccat agtgatttac ctgtacgtaa aggaaactta ctagataact
     4561 aatctggtca gttcatgtaa agtcacagat cttgtttatg tctctgtcta gaaatagctt
     4621 caggagccaa attacatgga ggaacttctg aaaaggggca ctggatgtgg aaagtggttg
     4681 gttggggtgg tgagagcact gtttctgaga agggtttgtt tattacgtct gactgtctgt
     4741 gctttactgt gctgtgcctt gcctgctctg atttcaagga ttctgtgagt ccttcagaag
     4801 catagagtaa ttccttttcc aggtctattt ttttttttga tgaacattct gtaaaaaact
     4861 gtcagcattc aagattatat ttaataataa attaagaatt atacttggac aaaa
//
