LOCUS       XM_039102531            5535 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus Rho guanine nucleotide exchange factor
            28 (Arhgef28), transcript variant X1, mRNA.
ACCESSION   XM_039102531
VERSION     XM_039102531.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051337.1) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_039102531.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..5535
                     /gene="Arhgef28"
                     /gene_synonym="Rgnef"
                     /note="Rho guanine nucleotide exchange factor 28; Derived
                     by automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 1 mRNA, 13 ESTs, 4 Proteins, and 100% coverage of the
                     annotated genomic feature by RNAseq alignments, including
                     91 samples with support for all annotated introns"
                     /db_xref="GeneID:361882"
                     /db_xref="RGD:1307061"
     CDS             201..5345
                     /gene="Arhgef28"
                     /gene_synonym="Rgnef"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 28
                     isoform X1"
                     /protein_id="XP_038958459.1"
                     /db_xref="GeneID:361882"
                     /db_xref="RGD:1307061"
                     /translation="MRRRRARLAGDAGTMELSCSEVPLYGQRTVYAKFGKNVYLPEDA
                     EFYFIYDGSHQRHVVIADRVEDNVLQSIIPGHWLQETVMVSVCLCSEGYSPVTMGSGS
                     VTYVDNMACRLARLLVTQADRLTPCSHQTLLTPFALTAEALPALDEELVLALTQLELP
                     LGWTVLGNSSLEVCLHRESLLHLAVRWALPKLFHFLLCLPGGVKALELPNEEATTPLD
                     LALHGGHSMLVEDISNFQGRRSPGFSRLRLNEDATLQFDRSSETLTLTVNHTAEHLLE
                     ADIKLFRKYFWDRAFLVKALDQEAKTERATMPSGAAETEEEVRNLESGRSPSEEEEDG
                     QLVKSQADGPSEQEDQDRLVLDHSFDGLKKSKHAPASLAAGQLSDVPNGGDEVYANCM
                     VIDQVGDLDINYINIEGLSTHTSPESMRSTLGPQACKHILPPDTSPCGHLLCEDSDRT
                     LDAAASQSCMSPPSSHTSNLNLSFGLHGFVKEQSHLKKRSSSLDALVADSEEEGRSEP
                     PICYAVGSQSSPRTGLPGGDELDSFDANTEPDCNISRTESLSLSSTLHSKESLLSGIR
                     SRSYSCSSPKISSGKSRLVRDFTVCSSSEEQRSYSFQEPPGEKRIQEEEWDDYIIPAK
                     AESEKHKVSRTFSFLMNRMTSPRNKSKMKNKDTKDKEKLNRHQFVPGTFSGVLQCSGC
                     DKTLLGKESLQCANCKANTHKGCKDTVPPCTKKFQDKYNKNKPQTILGSSSVRDVPPP
                     GLSLHPSPSMPIGLPAGRKETAGQAHPLSRSVPGTTLESFRRAVTSLESEGDSNSWRS
                     RSHSDELFQSMGSSPSTESFMMEDVVDSSLWSELSSDAQEFEAESWSLVVDPSFCSRQ
                     EKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDRIFPCLDELL
                     ETHKHFFFSMKERRQESCTGNDRNFVINQIGDILVQQFSEENASKMKRIYGDFCSHHK
                     EAMSLFKELQQNKKFQNFIKIRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYT
                     KERTEEHRDLCRALGLIKDMIAAVDLKVSEYEKNQKWLEILNKIENKTYTKLKNGHVF
                     RKQALMSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKP
                     SVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTSSKEERNNWMRRIQQAVESCP
                     EEEGGRTSESDEERRKADARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGF
                     EDVHLEPHLLIKPDPGEPPQAASLLAAALREAESLQVAVKASKLADVSQPSEEGPGGA
                     VLADTLSAQDAPASPTAFTEGTEGRGCWDVDPRLQGVVTDLAVSDAGEKVEYRSFSGS
                     SQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLVLQQQESLAPSHSFRGGPLQDQE
                     KSRYLEKHREELANIHKLQYQFQQEQRRWHRTCDQQQREQEAQESWLQARERECQSQE
                     ELLLRHRSELDHQLQEYQQNLERLREGQRMVERERQRMRDQQGLLGHCKHSRQRSLPA
                     VFSPGSKEVTELNRAESLCHEKSFFLNDAFTHMSLNTSNKPNPSGAPWDAHPPGGSHL
                     DLARTSDSQIEVKMDVSQPSDVNSELWTTGLGPQRPARQESIKDSCKNVADLNSFQTE
                     SPDPQDSNQRGPQPQTLITEAKLTPPMAAGHGGDAGDGAEENIVYL"
ORIGIN      
        1 ctagggcgtg gaggcccccg gggcgagcgg gcgccgccgc cgctagggtg caaccctgcc
       61 ctggcccagc aggtcccgct ccgtgcccgc cgcgggagga aagccgaggc caccggccgc
      121 cgccccgcga agggaggggg cggcggagcc gcagcagctt caggtttcct tctagagctg
      181 gtggcgggcg cggccatgtg atgaggcggc gacgagcccg gctggctgga gatgcaggaa
      241 ccatggagct gagctgcagt gaagtgcctc tttacggaca gaggacggtc tatgcaaagt
      301 tcggcaaaaa cgtttatctt cctgaagatg cggagtttta ctttatttat gatgggtcgc
      361 atcagaggca cgtggtgatt gcggatcgtg ttgaggataa tgttctccag tctatcattc
      421 caggccattg gcttcaagag acggtgatgg tatctgtgtg cctctgctca gaagggtatt
      481 ccccggtgac catgggctct ggctcagtga cctatgtgga caacatggcc tgcaggctgg
      541 ctcgtctgct ggtcactcag gctgaccgac tcacgccctg tagtcaccag acactgctga
      601 ccccgtttgc cctgacagca gaagcgctgc ctgccttgga tgaggaactc gtgctggctc
      661 tgacccaact ggagctgcct ttgggttgga cagtcctggg aaattcctca cttgaagtgt
      721 gtctacacag agagtctctc ctgcacctgg cagtgaggtg ggccctgcct aagctattcc
      781 atttcctctt gtgcctccct ggaggagtca aggccttgga gctacctaat gaagaggcca
      841 ccacaccatt agacttagcc ttgcatggcg gacactctat gctggtggaa gacatctcaa
      901 attttcaggg tcgccgctcc ccaggcttct cccgactgcg tcttaatgaa gatgccacgc
      961 tgcagttcga tcggtcatca gaaacgttga cgctgaccgt taaccacaca gctgagcatc
     1021 tgttggaagc agatatcaaa ctcttccgga agtatttctg ggacagagcc tttcttgtca
     1081 aggctcttga tcaagaagcc aagacagaga gagcaaccat gccttctgga gctgcggaaa
     1141 ctgaagaaga ggttaggaat ctggagtctg gcagatcacc ctcagaagag gaggaggatg
     1201 gacagctcgt caaaagccag gccgacgggc cgagtgaaca ggaagaccag gaccgcctag
     1261 ttttggatca ctcctttgat ggcctcaaaa agtccaagca cgccccagcg tcccttgctg
     1321 caggccagct ctctgacgtg ccgaatgggg gtgacgaagt ctacgctaac tgtatggtga
     1381 ttgatcaggt tggcgatttg gacatcaact atataaacat agagggactc tccacccata
     1441 ctagcccgga gtccatgagg tccactctgg ggcctcaggc ttgtaagcac atccttcctc
     1501 ctgacaccag tccctgcggg cacctgctct gtgaggacag tgacaggacc ctggatgctg
     1561 cagcctctca gtcctgcatg tcaccaccca gttctcacac ttccaacttg aacctttctt
     1621 ttggtctcca tggatttgta aaggagcaga gtcatctcaa gaaaagaagt tctagcctgg
     1681 atgccctcgt tgccgacagt gaagaggaag ggcgttctga gccccccatc tgctatgctg
     1741 tcggctctca gagttccccc agaactgggc tccccggcgg agatgaactg gattcttttg
     1801 atgcgaacac cgaaccagac tgtaacatct ctaggaccga atcactttcc ctgtccagca
     1861 ctctgcattc gaaggagtcc ctgctttctg ggatccgatc acgctcctac tcctgctcat
     1921 cacccaaaat ctcttcagga aaatcccggc tagtgcgcga tttcacagtg tgcagttcca
     1981 gtgaagaaca gagatcatat agtttccagg agccaccggg agaaaaaaga attcaggaag
     2041 aagaatggga cgactacatc attcctgcca aagcagagtc cgaaaagcac aaagtgagcc
     2101 ggactttcag cttcctcatg aacaggatga ccagtcctcg gaacaaatct aagatgaaaa
     2161 acaaggacac taaagataaa gaaaaactca atcgacatca gtttgtcccg ggaacgttct
     2221 ctggagttct gcagtgttcc ggttgtgaca aaacactcct ggggaaagag tcactccagt
     2281 gtgccaactg caaggccaac acacacaagg gctgcaaaga cactgtgcct ccgtgtacca
     2341 agaaattcca agacaaatac aacaagaaca aaccacagac catcctcgga agctcttcag
     2401 ttagagacgt cccaccgcct gggctctcct tgcacccttc cccctccatg cctattggac
     2461 tgccagctgg aaggaaggaa actgcagggc aggcacatcc gttgtccaga agtgtcccag
     2521 gcaccacttt ggaaagcttc aggagggcag tgacatcctt ggagtctgag ggtgacagta
     2581 acagctggag aagcaggtct cattccgatg agctgttcca gtccatgggc tcgtctcctt
     2641 ccacggagtc attcatgatg gaagatgtcg tggattcttc tctgtggagt gagctcagca
     2701 gtgatgctca agagtttgaa gctgagtcct ggagtctcgt ggtggaccct tccttctgca
     2761 gcagacagga gaaggatgtc atcaaaagac aggatgtcat ttttgagctg atgcagacgg
     2821 aggtgcacca catccagaca ctgctcatca tgtctgaagt cttcaggaag ggcatgaagg
     2881 aggagctgca gctggaccac agcacggtgg acaggatctt cccctgctta gatgagctgc
     2941 tggagactca caagcatttc ttcttcagca tgaaggagcg caggcaggag tcctgcaccg
     3001 gcaatgacag gaactttgtg atcaaccaaa tcggagacat tttggtacaa cagttttcag
     3061 aagaaaatgc aagcaagatg aagaggatct atggagattt ctgcagccat cacaaagaag
     3121 ccatgagcct ctttaaagag ctccagcaga acaaaaagtt tcagaatttt attaagatcc
     3181 gaaatagcaa cctgttggcc cgtcgccgtg gaatcccaga gtgcatcctg cttgtcaccc
     3241 agcgcatcac gaagtacccg gtcttggtgg agaggatcct gcagtacacc aaggaaagga
     3301 ccgaagagca cagagacctg tgtagagctc ttggcttaat taaagacatg atcgcagccg
     3361 tggacctgaa agtcagtgaa tatgagaaga accaaaaatg gctcgagatc ctaaataaaa
     3421 tcgagaacaa aacatacaca aagctcaaaa atggccacgt gtttagaaag caggcgctga
     3481 tgagccaaga aagggctctt ctgcacgacg ggctcgttta ctggaaaact gcgacaggcc
     3541 gtttcaaaga catcctggcc ctgcttctca ccgatgtgct gctcttcttg caagaaaaag
     3601 accagaagta tatctttgca gctgttgacc agaagccttc agttatttcc cttcagaagc
     3661 tcatcgctag ggaagtcgcc aacgaggaga gaggcatgtt tctcatcagt gcttcgtcag
     3721 cgggtcccga gatgtatgaa atccacacca gttccaaaga ggaacggaat aactggatga
     3781 ggcggatcca gcaggcagta gaaagttgtc cagaagaaga aggaggaagg acaagtgagt
     3841 ctgatgaaga gaggcggaaa gctgacgcta gagtggccaa aattcagcaa tgtcaagaga
     3901 ttctcagcaa ccaagaccag cagatctgca catatttgga agaaaagctg cacatctacg
     3961 ctgagcttgg ggaactaagt ggctttgagg atgtccacct agaaccccat ctgctcatca
     4021 agcccgaccc cggagaacct ccccaggcag cctccctgct tgcagcagcc ctgagagaag
     4081 ctgagagtct gcaagttgct gtgaaggctt caaagctggc tgatgtaagc cagccatctg
     4141 aagaaggccc tggaggggct gtcctggctg acacactcag tgcacaagat gcaccagcat
     4201 cgccgactgc cttcacagaa gggacagaag gaagaggctg ttgggatgtg gatccccggc
     4261 tccagggtgt ggtaacggac ttggcggtct ctgatgcagg ggagaaggtg gaatatagaa
     4321 gtttttccgg ttcttcacaa tcagagatta tacaagcgat acagaactta acgcgtctcc
     4381 tctacagcct tcaggctgcc ttgaccatcc aggacagcca cattgagatc cacaagctag
     4441 ttctacagca gcaggagagc ttggccccta gccactcctt ccgaggtggc cccttgcaag
     4501 accaggagaa atcccgttac ctggaaaagc atcgtgaaga gctggccaac atccacaagc
     4561 tccagtacca gttccaacaa gagcagcggc gttggcaccg cacatgtgac cagcaacagc
     4621 gtgagcagga ggcccaagag agctggctgc aggcacggga acgtgagtgc cagtcgcagg
     4681 aggaattact gctgcgccac cgtagcgaac tggaccacca actccaggag taccagcaga
     4741 acctcgagcg gttgcgggag ggccagagga tggtggagag ggagaggcag aggatgcgtg
     4801 accagcaggg tctgctgggc cactgtaagc atagtcgcca gaggagcctc ccagccgtct
     4861 tctctccggg gagcaaagag gtaacggaac ttaatcgagc tgagagtttg tgtcatgaaa
     4921 aatcattttt cctcaatgac gccttcacac acatgtcgct taacacatcc aataaaccaa
     4981 acccatcagg tgccccctgg gacgctcacc ccccaggtgg gtctcatctt gatttggcaa
     5041 ggactagtga tagtcagata gaagtgaaga tggacgtttc tcagccttcc gatgtcaact
     5101 ctgaactctg gacaacaggg ctgggccctc agagacctgc tcgccaagaa agcatcaagg
     5161 attcttgtaa aaatgttgca gatttgaact ccttccagac tgaatcccca gatccccagg
     5221 actcaaatca acgtggccct cagccacaga cactcataac agaagcaaaa ctaactcccc
     5281 cgatggcggc cggacatggt ggagatgctg gggatggagc tgaagagaac attgtttacc
     5341 tctgattgtg tggccatttg tttttccaag caaacaaaac cactgtcact ttggggagaa
     5401 cctttctttg gccccagtcc tcgtgtgtgt gtgattctct gtgcccgaat tgctttaaga
     5461 agaatattta atatttcatg tatgttcaat ttttgacatg agtctaataa ttaaattatt
     5521 aaaaaaaaaa ccagc
//
