LOCUS       NM_001013246            8490 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus Rho guanine nucleotide exchange factor 12
            (Arhgef12), mRNA.
ACCESSION   NM_001013246 XM_345930
VERSION     NM_001013246.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Chiu,W.C., Chiang,J.Y., Juang,J.M., Wu,C.K., Tsai,C.T., Tseng,Y.Z.,
            Su,M.J. and Chiang,F.T.
  TITLE     Reduction of blood pressure elevation by losartan in spontaneously
            hypertensive rats through suppression of LARG expression in
            vascular smooth muscle cells
  JOURNAL   J Formos Med Assoc 119 (1 Pt 1), 164-172 (2020)
   PUBMED   30962047
  REMARK    GeneRIF: reduction of blood pressure elevation by losartan in
            spontaneously hypertensive rats occurs through the suppression of
            LARG expression and MYPT-1 phosphorylation in vascular smooth
            muscle cells
REFERENCE   2  (bases 1 to 8490)
  AUTHORS   Xing,X.Q., Li,B., Xu,S.L., Liu,J., Zhang,C.F. and Yang,J.
  TITLE     MicroRNA-214-3p Regulates Hypoxia-Mediated Pulmonary Artery Smooth
            Muscle Cell Proliferation and Migration by Targeting ARHGEF12
  JOURNAL   Med Sci Monit 25, 5738-5746 (2019)
   PUBMED   31373336
  REMARK    GeneRIF: Results indicate that miR-214-3p acts as a novel regulator
            of hypoxia-induced proliferation and migration by directly
            targeting ARHGEF12 and dysregulating c-jun and c-fos in PASMC.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8490)
  AUTHORS   Artamonov,M.V., Momotani,K., Stevenson,A., Trentham,D.R.,
            Derewenda,U., Derewenda,Z.S., Read,P.W., Gutkind,J.S. and
            Somlyo,A.V.
  TITLE     Agonist-induced Ca2+ sensitization in smooth muscle: redundancy of
            Rho guanine nucleotide exchange factors (RhoGEFs) and response
            kinetics, a caged compound study
  JOURNAL   J Biol Chem 288 (47), 34030-34040 (2013)
   PUBMED   24106280
  REMARK    GeneRIF: Agonist-induced Ca2+ sensitization in smooth muscle:
            redundancy of Rho guanine nucleotide exchange factors (RhoGEFs) and
            response kinetics, a caged compound study.
REFERENCE   4  (bases 1 to 8490)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   5  (bases 1 to 8490)
  AUTHORS   Dubash,A.D., Wennerberg,K., Garcia-Mata,R., Menold,M.M.,
            Arthur,W.T. and Burridge,K.
  TITLE     A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA
            activity downstream of adhesion to fibronectin
  JOURNAL   J Cell Sci 120 (Pt 22), 3989-3998 (2007)
   PUBMED   17971419
  REMARK    GeneRIF: These data establish a previously uncharacterized role for
            the exchange factors Lsc/p115 RhoGEF and LARG in linking
            fibronectin signals to downstream RhoA activation.
REFERENCE   6  (bases 1 to 8490)
  AUTHORS   Yamada,T., Ohoka,Y., Kogo,M. and Inagaki,S.
  TITLE     Physical and functional interactions of the lysophosphatidic acid
            receptors with PDZ domain-containing Rho guanine nucleotide
            exchange factors (RhoGEFs)
  JOURNAL   J Biol Chem 280 (19), 19358-19363 (2005)
   PUBMED   15755723
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On Mar 16, 2021 this sequence version replaced NM_001013246.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY935257.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               JAXUCZ010000008.1  52372249-52372376   c
            129-152             JAXUCZ010000008.1  52319435-52319458   c
            153-238             JAXUCZ010000008.1  52317972-52318057   c
            239-295             JAXUCZ010000008.1  52315983-52316039   c
            296-394             JAXUCZ010000008.1  52302545-52302643   c
            395-444             JAXUCZ010000008.1  52301732-52301781   c
            445-502             JAXUCZ010000008.1  52298231-52298288   c
            503-681             JAXUCZ010000008.1  52295965-52296143   c
            682-759             JAXUCZ010000008.1  52294778-52294855   c
            760-879             JAXUCZ010000008.1  52294497-52294616   c
            880-1020            JAXUCZ010000008.1  52292593-52292733   c
            1021-1095           JAXUCZ010000008.1  52288486-52288560   c
            1096-1188           JAXUCZ010000008.1  52286615-52286707   c
            1189-1299           JAXUCZ010000008.1  52284436-52284546   c
            1300-1398           JAXUCZ010000008.1  52284027-52284125   c
            1399-1441           JAXUCZ010000008.1  52280403-52280445   c
            1442-1547           JAXUCZ010000008.1  52280016-52280121   c
            1548-1685           JAXUCZ010000008.1  52279470-52279607   c
            1686-1718           JAXUCZ010000008.1  52278859-52278891   c
            1719-1833           JAXUCZ010000008.1  52278467-52278581   c
            1834-1939           JAXUCZ010000008.1  52277560-52277665   c
            1940-2152           JAXUCZ010000008.1  52276075-52276287   c
            2153-2285           JAXUCZ010000008.1  52273179-52273311   c
            2286-2321           JAXUCZ010000008.1  52272741-52272776   c
            2322-2476           JAXUCZ010000008.1  52272074-52272228   c
            2477-2623           JAXUCZ010000008.1  52270788-52270934   c
            2624-2709           JAXUCZ010000008.1  52269806-52269891   c
            2710-2835           JAXUCZ010000008.1  52267269-52267394   c
            2836-2950           JAXUCZ010000008.1  52265363-52265477   c
            2951-3051           JAXUCZ010000008.1  52263124-52263224   c
            3052-3129           JAXUCZ010000008.1  52260886-52260963   c
            3130-3205           JAXUCZ010000008.1  52259594-52259669   c
            3206-3373           JAXUCZ010000008.1  52258937-52259104   c
            3374-3461           JAXUCZ010000008.1  52257541-52257628   c
            3462-3548           JAXUCZ010000008.1  52256800-52256886   c
            3549-3628           JAXUCZ010000008.1  52256609-52256688   c
            3629-3862           JAXUCZ010000008.1  52256113-52256346   c
            3863-4336           JAXUCZ010000008.1  52253774-52254247   c
            4337-4653           JAXUCZ010000008.1  52252693-52253009   c
            4654-4723           JAXUCZ010000008.1  52251311-52251380   c
            4724-8490           JAXUCZ010000008.1  52247047-52250813   c
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     gene            1..8490
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /note="Rho guanine nucleotide exchange factor 12"
                     /db_xref="GeneID:367072"
                     /db_xref="RGD:1305111"
     exon            1..128
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..4734
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /note="leukemia-associated Rho guanine nucleotide exchange
                     factor; Rho guanine nucleotide exchange factor (GEF) 12"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 12"
                     /protein_id="NP_001013264.2"
                     /db_xref="GeneID:367072"
                     /db_xref="RGD:1305111"
                     /translation="MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSTSHDF
                     DPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGA
                     AMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIRSGSYVALTVQGRPPGSPQIPLAESE
                     VEPSVIGHMSPIMTSPHSPGAAGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQE
                     RLQLLQEDYNRTATQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVIAPSRPLGDAL
                     TLSEAETDPGDGLCRTDWSSGDASRPSSDSADSPKSSLKERVCLEDTPERTEGVQDAD
                     TQSLVGSPSTRGAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHV
                     VSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLRVSVPEEISVDLEK
                     RRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKD
                     RGTLEKERACAEQIVTKIEEVLMTAQAVEEERSSTMQYVILMYMKHLGVKVKEPRNLE
                     HKRGRIGFLPKIKQSMKKDREGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMEIQKL
                     RHPKHLSAPSSMSPELQDPAKLRQSGVANEGTDTGYLPASSMSSATSGAALSQEGGRE
                     NDTGAKQVGDAVAPGDCLDSIPRVPNTVFDFPPPLLDQVQEEECEVERVAEHGTPKPF
                     RKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPSEIKRQEVINELFYT
                     ERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHVGLNEQMKAVRKRN
                     ETSVIDHIGEDLLLWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQD
                     AESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPPEREKVKKAADQCRQILN
                     YVNQAVREAENKQRLEDYQRRLDTSSLKLSEYPNVDELRNLDLTKRKMIHEGPLVWKV
                     NRDKSIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVR
                     QVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQ
                     PPPCEGDNDEEEPAKLKVEHHDLSVAGLQSPDRVLGLESPLISSKPQSHSMNTPGKSE
                     AEHLFVTATQFAKEQHANGTFKEGDGGYPVTISDPHLPVSEERWALDALRNLGLLKQL
                     LVQQLGLTEKTTQEDWRSFSRYRPASEEAQADSGIPDLESVKACHAGEGQMSFKTGTG
                     DVATCDSPRTSTESCAVQDSVILASQDSQASNIIEMDHMLLTPEVPTTAEPEGGLDDS
                     GEHFFDAREAHSDDNPSEGDGAVRKEEEDVNVRISGNCLILDGYDAVQESSTDEEIAS
                     SFTLQPVTGLPSVDSSHQQQHSPQNAISEGAISPSTPEFLVQRRWRAVEDACSEVQSP
                     SAWTDSQSQILEYIHKIEADLEHLKKVEESYTLLCQRLAGSALPDKVSDKS"
     exon            129..152
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            153..238
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            239..295
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            296..394
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            395..444
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            445..502
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            503..681
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            682..759
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            760..879
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1020
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1095
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1188
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1299
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1398
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1441
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1547
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1685
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1718
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1833
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1939
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2152
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2285
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2321
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2476
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2623
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            2624..2709
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            2710..2835
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            2836..2950
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3051
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            3052..3129
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3205
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            3206..3373
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            3374..3461
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            3462..3548
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            3549..3628
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            3629..3862
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            3863..4336
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            4337..4653
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            4654..4723
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
     exon            4724..8490
                     /gene="Arhgef12"
                     /gene_synonym="Larg"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aattgggcct gatcccagag tactgggggt ggggtgtgga ggtgttactg taaaatgcaa
       61 gttggataaa aagaagacct ctcgccaagg gccccaatga gtggcacaca gtctactatc
      121 acggacaggt ttcccctcaa aaaacctata aggcatggaa gtattttgaa ccgagagtca
      181 ccaacagata agaaacagaa agtcgaacgc agcacatcac atgattttga ccccacagat
      241 agctcctcca agaagacaaa gtctagttca gaggagagta gatccgagat ctatggtctt
      301 gttcagcgct gtgtgatcat ccagaaagat gacaatggat tcgggctgac agtcagcggg
      361 gacaatccag tcttcgtaca atctgtcaaa gaagatggag cagccatgcg cgctggagta
      421 caaacaggtg atcgaatcat caaggtgaat gggactctgg tgactcattc aaatcatctg
      481 gaggtggtga aactaatcag atctggttcc tatgtagctc tcactgtcca gggacgccca
      541 cctgggtcgc cccagattcc acttgctgag tctgaagtag aaccatcagt cattggacat
      601 atgtctccca ttatgacatc ccctcattcc cctggagcag ccgggaacat ggagagaatc
      661 actagtcctg tgctcatggg ggaggaaaac aatgtggtcc ataaccagaa agtagagatt
      721 ctgaggaaaa tgttacagaa agaacaggag cgtctgcagt tactgcagga agattacaac
      781 cgaacggcta cccagagatt gctcaaggag atccaggagg ccaagaagca catcccccag
      841 ctgcaggagc agctgtccaa agccacaggc tctgctcagg acggagctgt aattgcaccc
      901 tccaggccat taggtgatgc cctaacactc agcgaggcag aaacggaccc tggagacggc
      961 ctgtgcagga ctgactggag cagtggagat gcttctcggc ccagtagtga cagtgcagat
     1021 agccccaaga gtagcctgaa ggaaagggtt tgtctcgagg acaccccaga gaggactgaa
     1081 ggagttcagg atgctgacac acagtcgctg gtcggcagtc cctccacccg tggcgcccct
     1141 catattatcg gagcagagga tgatgatttt ggtactgaac acgagcagat caatggacag
     1201 tgcagctgtt tccagagcat tgagttgctg aagtctcgcc ctgcccactt ggctgttttc
     1261 ttacaccatg tagtctcaca gtttgaccct gcaacattgc tctgttatct ctattcagac
     1321 ctgtataagc agacgaactc caaggagact cggcgggtct tcctggagtt tcatcagttc
     1381 ttcttggacc gatctgcaca cctgagagtt tccgtccccg aggaaatctc tgtggatcta
     1441 gaaaagagaa gacccgagct cattcctgag gatctgcatc gccattacat tcaaacaatg
     1501 caggagaggg ttcaccctga agttcagagg cacttggagg attttcgaca gaaacgcagc
     1561 atgggactga ccttggctga gagtgagctg accaaacttg atgcagagag agacaaggac
     1621 cggggaactc tggagaagga gcgagcatgt gcggaacaga tcgttaccaa aattgaagaa
     1681 gtattgatga ctgctcaggc tgtagaagaa gaaaggagtt ccaccatgca gtatgtaatt
     1741 ctcatgtata tgaagcattt gggagtaaaa gtgaaagaac ctcgaaactt ggagcacaag
     1801 cggggtcgga tcggcttcct tccgaagatc aagcaaagca tgaagaaaga tagagagggg
     1861 gaagaaaaag ggaagcgaag aggattcccc agcattttgg gacccccaag aagaccaagc
     1921 cgccatgaca acagtgcaat tggcagggcc atggagatac agaagcttcg ccacccgaag
     1981 cacttgtccg caccctcatc tatgagtcct gagcttcagg accctgctaa gttgcgccag
     2041 agtggagtag ccaatgaagg aacagacact ggatatctgc ctgccagttc catgtcctct
     2101 gccacctcag gggctgctct ttcccaagag ggagggagag agaatgacac aggagcaaaa
     2161 caagttggag acgcggtggc acctggagac tgcttggata gcatacctcg agtccccaat
     2221 actgtttttg acttcccccc gcctctgcta gatcaagtgc aggaggagga atgtgaagtg
     2281 gagagggtgg cagaacatgg aaccccaaaa ccctttcgaa agtttgacag catcgccttt
     2341 ggagaaagtc agagtgagga cgagcagttt gaaaatgacc tggaaacaga tccacccaac
     2401 tggcagcagc tcgttagcag agaagtgcta ctgggactga aaccctccga gatcaagaga
     2461 caggaagtga ttaatgaatt gttctacact gagagagcgc atgtccgaac actgaaggtt
     2521 cttgaccaag tattctatca gcgagtgtcc agggaaggga ttctatcacc ttccgagctg
     2581 cgcaagattt tttcaaactt ggaggatatc cttcaacttc atgttgggtt gaatgagcag
     2641 atgaaagctg tccgaaaaag gaacgagacc tcagttattg atcatattgg ggaagatctg
     2701 ctcctttggt tcagtggacc aggggaggag aagctgaagc acgctgctgc cacattctgc
     2761 agtaaccagc ctttcgccct ggaaatgatc aagtctcgcc agaaaaagga ttctcgattc
     2821 cagacttttg tgcaagatgc tgagagtaac cccctgtgcc gtcgtcttca gctgaaggat
     2881 atcattccca cccaaatgca gaggctcacc aagtaccccc ttcttttgga taatatcgcc
     2941 aaatacacag aatggcctcc agaaagggaa aaggtgaaga aggctgcaga tcaatgtcgt
     3001 cagatcttaa attatgtaaa tcaggctgtc agggaggcag aaaacaagca gcgcttagaa
     3061 gactatcagc gtcgccttga cacctccagt ctcaagttgt ctgagtaccc gaatgttgat
     3121 gagctcagga atttggattt aacaaaaaga aagatgattc atgaagggcc gttggtctgg
     3181 aaggtgaata gagataaaag catcgatctg tatacactgc tgctggaaga cattcttgtg
     3241 ctgttacaga agcaggacga caggctggta ctgaggtgcc acagcaagat cctggcctcc
     3301 acagctgaca gcaagcacac cttcagtcct gtcattaagc tcagcacagt gctggtccga
     3361 caggtggcca cagataacaa agctttattc gtcatctcca tgtcagataa tggagcccaa
     3421 atttatgaat tggtagcaca gacggtttct gaaaagacgg tctggcaaga cctcatctgt
     3481 cggatggccg catcagtgaa ggagcagtcc acaaagccga ttcccctccc acagccgcca
     3541 ccttgcgaag gagacaatga tgaagaagaa ccagccaagt taaaagtgga gcaccatgat
     3601 ctttctgtcg ctggtctgca gagtccagac agagttttgg gattagaatc tcccttaatt
     3661 tcgtcaaaac ctcagtccca ttcaatgaat acccctggca aatcagaggc agaacatctc
     3721 tttgtgaccg caacacagtt tgcaaaggag cagcatgcaa atgggacctt caaggaaggt
     3781 gatggcgggt atccagtcac aatctcagat ccacacttgc ccgtgtcaga ggagcggtgg
     3841 gcattggacg cactgagaaa tttgggcttg ttgaagcagt tgctggttca gcagttgggg
     3901 ctcacggaga agactactca ggaagactgg cggtccttct caagatacag accggcttct
     3961 gaagaggcac aggcggacag tggcattccg gacttggaaa gtgttaaggc ttgtcatgcc
     4021 ggggaaggac agatgtcttt taaaactgga actggtgatg ttgcaacttg tgacagtcca
     4081 cggacttcaa ctgagtcctg tgctgtacag gattcggtga tactggcttc ccaagacagc
     4141 caggcaagca acatcataga aatggaccac atgctcctga ccccagaggt gcctactact
     4201 gcagagccag aggggggtct ggatgacagt ggagagcact tttttgatgc ccgtgaagca
     4261 catagcgatg ataatccatc tgaaggtgat ggcgcagtca gaaaggaaga ggaggatgtt
     4321 aatgtgcgca tctcaggaaa ctgtttgatc cttgatggct atgatgcagt gcaggagagc
     4381 tccacagatg aggagattgc ctcctcattc accctgcagc ctgtgacagg cctcccttct
     4441 gtggactcca gccaccagca gcaacactcc cctcagaatg ccatttccga gggggcgatt
     4501 tcaccatcca ccccagaatt cctggtccag cgacgctgga gagctgtgga ggatgcttgc
     4561 tctgaggtcc agagtccctc tgcttggaca gattcacaaa gccaaatcct ggagtacatt
     4621 cataaaatag aggctgacct ggaacactta aagaaggtgg aggaaagtta cacccttctt
     4681 tgccaaaggc tggctggctc agccctcccc gacaaggtct cagataaaag ttagagcccc
     4741 gtgtcctgga ggtgactgca ggttttggat tttgaatgtc ggccctgtat caaccacatc
     4801 ccggctccag tggacacaaa gagagtgtga cagaggatct gcctggaacc acctgggctc
     4861 agccacgccc cagggtgccc ccagtgggac tagttcttca ttctggcagc tgcacacatc
     4921 tgtcagtgag ggaatgtcag gtctctcact ctcctctcct cactatcaga aatgcatttt
     4981 gattcagaat aaaaaccaaa tgtatagagc tttgggtgta ggatatgaaa ttgtacgtag
     5041 gtaagaaaaa gagaaaatca gatgtattta tttgacttca ttccatattt gaaagcacac
     5101 tttaattttt actttgcctt gttttgtttt taattgagta gtgagagttt ttgccctttg
     5161 tatttagcac acccaaggat caattgttcc tgaagccaag gtcaggtcgg tcagggatgg
     5221 agaggttagt agacaggcaa tgcagaagag gaaagaggac tacctggccc tcactaacct
     5281 gtaaccccgc cctcctgtcc agaaggctgc cttcctgagg gtcatctcag aggtgccgca
     5341 tgactcctgt gtgtggatga cacagctcta gggttccacc ccaggaaaca agagtcctca
     5401 gtacactaaa tggcaggcac ttgaaaatgg gtgtatccct tgttgttgtc tgttctgtgg
     5461 atagttgggc tttgggagag aagaaaacaa atttttattt tcttgattat aaatttgtta
     5521 ttcttggtat tgttgcattt gtataattac gcgttgactg gcacttgtat gaaatattcc
     5581 ctgcacagtg agcaggaaat aggaacaaag aaatggaatg cttcagtggt ggtgggtgct
     5641 gcagaggggt ggggtgggcg ggagaggagt ccgagaaggt tttggaaact gaatcactac
     5701 tgagttgaac catggctgtc actagccacg ggtactgctg attataaggc cagtaaaaaa
     5761 aattagtacc tggatgtttg actctaattt tttgcactga tggtgccaaa gggctctgag
     5821 tcccatgcgg agctgaggga gggcagagga aagtgtgaag gagagctgct cgcatagcat
     5881 cgcttcagag tgctttctct gacctcactg catggccctg tctgtatggc gccagaactg
     5941 gccttgggag caggggacag cgctgagcag cgaagcctcc gaggtgctga cactgaggtg
     6001 ctgacgccgc tggtggctgc agtgtgagca gcacctgtgg agggcaagct ggtccgagct
     6061 gcgacctgca gctccttgat tctgatggtg ttcagtggct ttctttgtgc atttggtaaa
     6121 tatgtttttg gagtgttctg taaaaaagaa aaaaatcatt tgtcaaatct aaattctgtt
     6181 aagaaggagt tcacatgttg cttacatcat gcaggggggg ggcctctgaa ctctgggggc
     6241 atcaggatgt aatttattct ccccttaatg cccctctaca caaaggaagt gcattgttgc
     6301 caaggatagc cccccagctg ctcttatgaa cacctctaca acttaacaag ctcatcttct
     6361 gggtccagat tttcgtcttt gcccttgaga atgccactga gttcttaggt cgatcggcca
     6421 ggatccctgt acctcatcta caaattggaa tcatattttg agtcatagtc aaccataact
     6481 cataatgggg ctttgaaatt ttggagtaga acctaatata taagtgaagt ttgggatttg
     6541 gaactccctg tatctcttag gagaagcaag tagagaccga atggttacgt tgtcttccag
     6601 gcagcctctt acactacacc atctgggtag ctcatcagga gaaaatgcag acagcagtgt
     6661 tccgtcttca cacactacag tccctccagg gtgaccgagc gcagctcagc cggaaccctg
     6721 ttctcctgga tgtttacttt tctggatctc agcctctccg agttacagca gggctcactg
     6781 gaaaggtgct tcggcattgc tccagcactt gcaaataagt gcatgtcata tcatcacatc
     6841 ttcacatgaa acctcaaaat gaagaacaac accaacaaac cacttgtgag agctttttgt
     6901 ttcgtttcgt ttcgttttgt tttgttttta atctatcagc cttacttctt gcctgtcccc
     6961 tccctctctg gcctcccgtg ccttccattt cattttggcc attagaatca catccgtttg
     7021 actttgacat tgagtctccc ttccatgttg gtgacaacag atattccccc tgtcctggaa
     7081 tgagatggtc attgtcatag tcccctcaaa ggtgggtgac aagaaggtgc tgtgggtgaa
     7141 cttctgccct gaagagcgct aggcaagcaa gggggctcag catcttctgc agagccagaa
     7201 agccatgttt gcgatgggtg caacctgtga ccctgctctc ttgtgagcat agtggctctt
     7261 ttgtcactaa atggcaggtt atatgtgatg tgtcaccctt tcagagaaaa gacctaacca
     7321 aaccaaacca gcaaaccctt caagtactgt aatttaaagg atggctgtga ccccccaccc
     7381 ccagtctagt ttcatagcag ctaactggac tgtcggagct cagctgctct cacaacctaa
     7441 attgtaacta tcgatctgtc atgtggcagg ttgttccatg aatatttttt cctttgtttt
     7501 cccccaacaa cttttaatga cctctagaaa gaacaagtcc tcttttagaa agtattttgt
     7561 ttctttgtct ttgggggagg gaagaagaga aaagtccttg ttgccggaat tttttaagaa
     7621 gctaaagact ttaagtatgc tgcctctgat gctgtccgtc tactttccag tctgctcctt
     7681 ggcattgcca aggcctgaga ctggcactct gcccaagcag cctctctgca gccacctgag
     7741 ctggctctgt acttctttcc ctccatgtgg cagaaggcac caaaggtcct caaatacaaa
     7801 cttagcgaca ccttgcagaa gtttctgtcc tgtagatcat ggtttctttt tttttctttt
     7861 ttttttttct ttttttcttt ttctttcttt ctttctttct tttttttttt tttttttttt
     7921 tgacatttca ccccccattt actgtgatct gtaaaattgt tttttgttta aaggagcgct
     7981 ctccttctgt ctggtcgcct tccccatgga taccgagggt ttgggtaata atccgttgat
     8041 caggaaaaag gggcttctca gaaaccagtc cctcccagcc atttccaggt gccacccgtg
     8101 aagcagacat acacagcacg gtgaccgcag cgtgcacact gttggttgtt tttaatgaac
     8161 ctcttcccac taggacgtct cactttctcg ttcaccatac aatcatgtac tctttaacag
     8221 aaattgcttt taagagaaat ctggaactat ctttaaaaga aaagaacctt attaataatc
     8281 atgtattttt actgatcaca ttttgaaatg cctaaaagac ttttattgct ctaattatcc
     8341 agatgcacct ttgtaaaaag ctcttttatg aattagctga taagactgta tgtttctgga
     8401 acaaaatatt gggtcatcta aaactttctg ttttctgggg tctgggaaaa tagaaaataa
     8461 gaattcaaat attaaataag cttaaaagaa
//
