LOCUS       NM_021694               3243 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus Rho guanine nucleotide exchange factor 1
            (Arhgef1), mRNA.
ACCESSION   NM_021694 XM_346535
VERSION     NM_021694.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   MacKay,C.E., Shaifta,Y., Snetkov,V.V., Francois,A.A., Ward,J.P.T.
            and Knock,G.A.
  TITLE     ROS-dependent activation of RhoA/Rho-kinase in pulmonary artery:
            Role of Src-family kinases and ARHGEF1
  JOURNAL   Free Radic Biol Med 110, 316-331 (2017)
   PUBMED   28673614
  REMARK    GeneRIF: Reactive oxygen species and SrcFK activate RhoA via
            ARHGEF1 in pulmonary artery.
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   3  (bases 1 to 3243)
  AUTHORS   Varma,H., Yamamoto,A., Sarantos,M.R., Hughes,R.E. and
            Stockwell,B.R.
  TITLE     Mutant huntingtin alters cell fate in response to microtubule
            depolymerization via the GEF-H1-RhoA-ERK pathway
  JOURNAL   J Biol Chem 285 (48), 37445-37457 (2010)
   PUBMED   20858895
REFERENCE   4  (bases 1 to 3243)
  AUTHORS   Porchia,F., Papucci,M., Gargini,C., Asta,A., De Marco,G.,
            Agretti,P., Tonacchera,M. and Mazzoni,M.R.
  TITLE     Endothelin-1 up-regulates p115RhoGEF in embryonic rat
            cardiomyocytes during the hypertrophic response
  JOURNAL   J Recept Signal Transduct Res 28 (3), 265-283 (2008)
   PUBMED   18569527
  REMARK    GeneRIF: results indicate that ROCK and its downstream target
            molecules, which are involved in inducing the hypertrophic
            response, are also implicated in signaling the up-regulation of the
            p115RhoGEF protein.
REFERENCE   5  (bases 1 to 3243)
  AUTHORS   Dubash,A.D., Wennerberg,K., Garcia-Mata,R., Menold,M.M.,
            Arthur,W.T. and Burridge,K.
  TITLE     A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA
            activity downstream of adhesion to fibronectin
  JOURNAL   J Cell Sci 120 (Pt 22), 3989-3998 (2007)
   PUBMED   17971419
  REMARK    GeneRIF: These data establish a previously uncharacterized role for
            the exchange factors Lsc/p115 RhoGEF and LARG in linking
            fibronectin signals to downstream RhoA activation.
REFERENCE   6  (bases 1 to 3243)
  AUTHORS   Eisenhaure,T.M., Francis,S.A., Willison,L.D., Coughlin,S.R. and
            Lerner,D.J.
  TITLE     The Rho guanine nucleotide exchange factor Lsc homo-oligomerizes
            and is negatively regulated through domains in its carboxyl
            terminus that are absent in novel splenic isoforms
  JOURNAL   J Biol Chem 278 (33), 30975-30984 (2003)
   PUBMED   12773540
REFERENCE   7  (bases 1 to 3243)
  AUTHORS   Garcia-Mata,R. and Sztul,E.
  TITLE     The membrane-tethering protein p115 interacts with GBF1, an ARF
            guanine-nucleotide-exchange factor
  JOURNAL   EMBO Rep 4 (3), 320-325 (2003)
   PUBMED   12634853
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC091218.1.
            
            On Apr 20, 2005 this sequence version replaced NM_021694.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC091218.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..3243
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /note="Rho guanine nucleotide exchange factor 1"
                     /db_xref="GeneID:60323"
                     /db_xref="RGD:620951"
     exon            1..95
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            96..137
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..2873
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /note="lbc's second cousin; Rho guanine nucleotide
                     exchange factor (GEF) 1; Lymphoid blast crisis-like 2"
                     /codon_start=1
                     /product="rho guanine nucleotide exchange factor 1"
                     /protein_id="NP_067726.3"
                     /db_xref="GeneID:60323"
                     /db_xref="RGD:620951"
                     /translation="MGEVAGGAAPGPPRSGLVSIIIGAEDEDFENELEANPEDQNSQF
                     QSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFYHSFL
                     EKTAVLRVPVPPSVAFELDRTRPDLISEDVQRRFIQEVVQSQQAAVTRQLEDFRSKRL
                     MGMTPWEQELSLLEPWIGKDRGNYEARERHVAERLLSHLEEMQHTISTDEEKSAAVVT
                     AISLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKTKKGLSSILDPARWNRGE
                     PSAPDCRHLKVEVDEKPGPADRKGSLGISSRDRTVGTPGQDNPGVSLHPLSVDSLDSR
                     EPGVDTPQEPGDTPPQGPTSLEPLAPPESTEDNGETESPEPGDDGEPGRSGLEQEPEE
                     PPGWRELVPSDTLLGLPKNQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMAEGGFF
                     PLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQK
                     ISSRFCSRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKY
                     PLLLQSIGQNTEEPAERAKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTH
                     LRQSNDPMLSEFKNLDITKKKLVHEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDER
                     LLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVA
                     QTSSERKSWCALITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGTGGTEAAPA
                     DARTERILNDLLPFCRPGPEGQLAATALQKVLSLKQILLSTEEDSGAGPPRDGDGVPG
                     GGAPGPTHTQEVEENLLSLEVVIKQLEELEEEFCRLRPFLSQLGEILSPNLAAPERSA
                     QTGLS"
     exon            138..218
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            219..332
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            333..431
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            432..474
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            475..721
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            722..751
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            752..851
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            852..948
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            949..984
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1122
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1228
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1374
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1521
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1604
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1730
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1845
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1946
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2024
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2100
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2268
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2356
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2357..2443
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2511
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2598
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2599..2762
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2763..2862
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
     exon            2863..3210
                     /gene="Arhgef1"
                     /gene_synonym="Lbcl2; Lsc"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgctggagcc aggaagccgg agtcgggacg ctgggccggg atcgccgagc ccgacctcgg
       61 gcgccccgcc ggtcacctcc ccgcgcggac accagccccg tggagtcctg gagatgggag
      121 aagtcgccgg aggggcggcc ccagggcctc cccggtctgg cctggtgtcc atcatcatcg
      181 gggctgagga tgaggacttt gagaatgaac tggaggcgaa cccagaagat caaaacagcc
      241 agttccagag cctggagcaa gtgaagcgtc gcccagccca cctcatggcc ctcctgcaac
      301 acgtggccct gcagttcgag ccaggaccac tgctctgctg cctgcatgct gacatgctaa
      361 gctcactggg ccccaaagaa gccaagaagg ccttcctcga tttctatcac agtttcctgg
      421 agaagactgc ggttctgcgg gtgccggtcc ctcccagcgt tgcttttgaa cttgatcgta
      481 ctcggcctga tctcatctct gaggatgtcc agaggcggtt catacaagag gtggtacaga
      541 gccagcaggc agccgtgacc cgtcagctag aggacttccg ctccaaacgg ctcatgggca
      601 tgacaccctg ggagcaggaa ctgagcctgc tagagccctg gattgggaaa gaccggggca
      661 actatgaggc ccgggagcgg catgttgcag agcggctgct ctcccaccta gaggaaatgc
      721 aacataccat ctccacagat gaagaaaaaa gtgctgctgt ggtcactgca atcagcctgt
      781 atatgcgcca ccttggggtc cggaccaaga gtggggacaa gaagtcaggg aggaacttct
      841 tccggaaaaa ggtgatgggg aatcggcggt cagacgagcc ccccaagaca aagaaagggc
      901 tgagcagtat cctagatcct gcccgctgga accggggaga gccatctgct ccagactgtc
      961 gacatctaaa ggtcgaggtt gatgagaagc caggccctgc agaccggaag ggaagcctgg
     1021 gtatttcatc tcgggacagg actgttggga ctcctgggca ggacaaccca ggagtctccc
     1081 tgcaccctct gtctgtagac agcctggatt cccgggaacc aggtgtggat accccgcagg
     1141 agccaggaga tacaccccca caaggcccta caagcctgga gcccctggca cccccagaga
     1201 gcacagagga caatggcgag actgaaagcc ctgagcctgg agatgatgga gagccagggc
     1261 ggtcaggcct ggaacaggaa ccagaagaac ctcctgggtg gagggaactg gtgccctcag
     1321 acaccctgct cggcctgccc aagaaccaag tgaagcggca agaggtcatc agtgagctgc
     1381 tggtgacgga ggcggctcac gtccgcatgc tacgggtact acatgacctc ttctaccagc
     1441 ccatggcaga agggggcttc ttccctctgg aggaactgca gaacatcttt cccagcctgg
     1501 atgagctcat cgaggtgcac tccctgttcc tcgatcgctt gatgaagcgg agacaagaga
     1561 gtggctacct cattgaggag attggtgatg tgctactggc ccggttcgat ggtgctgagg
     1621 gctcatggtt ccagaagatc tcttcccgct tctgcagccg ccagtcattc gctctagagc
     1681 agctcaaagc caagcagcgc aaggagcctc gattctgtgc ctttgtgcag gaagctgaga
     1741 gccgcccccg ctgccggcgg ctgcagttga aggacatgat ccccactgag atgcagcggc
     1801 tgaccaagta cccactgctg ctgcagagta ttgggcagaa cacagaagag cctgccgagc
     1861 gggcaaaggt ggaacttgca gctgagtgct gccgtgagat tctgcaccac gttaatcaag
     1921 ctgtccgtga catggaggac ctgctgcggc tcaaggatta ccagcggcgc ctggacttga
     1981 ctcacctgcg gcagagcaat gacccaatgc tgagcgagtt caagaacctg gatatcacta
     2041 agaagaagtt ggtccatgaa ggccccctca cgtggcgact aaccaaagac aaagctgtag
     2101 aggtccacgt gctattgctg gacgacctgc tgctgctgct ccagcgccag gatgagaggc
     2161 tgctgctcaa gtcacacagc cggacgctga cacccacacc cgacggcaag accatgctgc
     2221 ggcctgtgct ccggctcacc tctgccatga cccgggaggt ggccactgat cacaaagctt
     2281 tttacgtcat ttttacctgg gaccaggagg cccagatata tgagctggtg gcacagacat
     2341 cgtcggaacg caagagctgg tgtgccctca tcacagagac tgccggatcc ctgaaggtcc
     2401 ctgcccctgc ctcccgcccc aaaccccggc ccagcccaag cagcacccga gaacccctgc
     2461 tcagcagctc tgagaatggc actggaggca cagaggccgc tccagctgat gcccggacag
     2521 agcggatcct caacgacctc ctgcccttct gcagaccagg cccagagggc cagcttgctg
     2581 ccacagccct ccagaaagta ctgtccctga aacagatcct gttgagcact gaggaagaca
     2641 gtggagcagg gcctccccgc gatggggatg gggtgcctgg tggtggtgcc ccaggcccaa
     2701 cacacaccca ggaggttgag gaaaacctgt tgagcctaga agtggtcatc aaacaactgg
     2761 aggagttaga agaggaattt tgtcgcctaa gacccttcct gtctcagctt ggggagattc
     2821 tgtcccccaa cctggctgca cctgagcgct ctgctcagac aggcctttca tgaagagaag
     2881 aatgggggag aggatgcaag gggcccccaa gccccacagc ttccatagca tcacacccca
     2941 ggggctttga gaagaggaga ttggctgaac ttgatagtgg acctgctcgg gtccctgcct
     3001 cccaccctgg cctctgcctt tctccctcct gccttctgct tgggggactc aggacttcac
     3061 ttccagagta ccctcataat ccccattttt caggccacct cagtattgcc tatggggagg
     3121 ggtcacccct ccattcccta ccccaagtgc ctttgctgtt tttatatcct gaattggagg
     3181 tttattttta atatatatta tctaaggtga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3241 aaa
//
