LOCUS       NM_181083               5784 bp    mRNA    linear   ROD 02-FEB-2024
DEFINITION  Rattus norvegicus ADP ribosylation factor guanine nucleotide
            exchange factor 2 (Arfgef2), mRNA.
ACCESSION   NM_181083
VERSION     NM_181083.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Boal,F. and Stephens,D.J.
  TITLE     Specific functions of BIG1 and BIG2 in endomembrane organization
  JOURNAL   PLoS One 5 (3), e9898 (2010)
   PUBMED   20360857
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   3  (bases 1 to 5784)
  AUTHORS   Islam,A., Shen,X., Hiroi,T., Moss,J., Vaughan,M. and Levine,S.J.
  TITLE     The brefeldin A-inhibited guanine nucleotide-exchange protein,
            BIG2, regulates the constitutive release of TNFR1 exosome-like
            vesicles
  JOURNAL   J Biol Chem 282 (13), 9591-9599 (2007)
   PUBMED   17276987
REFERENCE   4  (bases 1 to 5784)
  AUTHORS   Shen,X., Xu,K.F., Fan,Q., Pacheco-Rodriguez,G., Moss,J. and
            Vaughan,M.
  TITLE     Association of brefeldin A-inhibited guanine nucleotide-exchange
            protein 2 (BIG2) with recycling endosomes during transferrin uptake
  JOURNAL   Proc Natl Acad Sci U S A 103 (8), 2635-2640 (2006)
   PUBMED   16477018
REFERENCE   5  (bases 1 to 5784)
  AUTHORS   Xu,K.F., Shen,X., Li,H., Pacheco-Rodriguez,G., Moss,J. and
            Vaughan,M.
  TITLE     Interaction of BIG2, a brefeldin A-inhibited guanine
            nucleotide-exchange protein, with exocyst protein Exo70
  JOURNAL   Proc Natl Acad Sci U S A 102 (8), 2784-2789 (2005)
   PUBMED   15705715
REFERENCE   6  (bases 1 to 5784)
  AUTHORS   Li,H., Adamik,R., Pacheco-Rodriguez,G., Moss,J. and Vaughan,M.
  TITLE     Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited
            guanine nucleotide-exchange protein 2 (BIG2)
  JOURNAL   Proc Natl Acad Sci U S A 100 (4), 1627-1632 (2003)
   PUBMED   12571360
REFERENCE   7  (bases 1 to 5784)
  AUTHORS   Shinotsuka,C., Waguri,S., Wakasugi,M., Uchiyama,Y. and Nakayama,K.
  TITLE     Dominant-negative mutant of BIG2, an ARF-guanine nucleotide
            exchange factor, specifically affects membrane trafficking from the
            trans-Golgi network through inhibiting membrane association of AP-1
            and GGA coat proteins
  JOURNAL   Biochem Biophys Res Commun 294 (2), 254-260 (2002)
   PUBMED   12051703
REFERENCE   8  (bases 1 to 5784)
  AUTHORS   Zhao,X., Lasell,T.K. and Melancon,P.
  TITLE     Localization of large ADP-ribosylation factor-guanine nucleotide
            exchange factors to different Golgi compartments: evidence for
            distinct functions in protein traffic
  JOURNAL   Mol Biol Cell 13 (1), 119-133 (2002)
   PUBMED   11809827
REFERENCE   9  (bases 1 to 5784)
  AUTHORS   Yamaji,R., Adamik,R., Takeda,K., Togawa,A., Pacheco-Rodriguez,G.,
            Ferrans,V.J., Moss,J. and Vaughan,M.
  TITLE     Identification and localization of two brefeldin A-inhibited
            guanine nucleotide-exchange proteins for ADP-ribosylation factors
            in a macromolecular complex
  JOURNAL   Proc Natl Acad Sci U S A 97 (6), 2567-2572 (2000)
   PUBMED   10716990
REFERENCE   10 (bases 1 to 5784)
  AUTHORS   Togawa,A., Morinaga,N., Ogasawara,M., Moss,J. and Vaughan,M.
  TITLE     Purification and cloning of a brefeldin A-inhibited guanine
            nucleotide-exchange protein for ADP-ribosylation factors
  JOURNAL   J Biol Chem 274 (18), 12308-12315 (1999)
   PUBMED   10212200
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY255526.2.
            
            [WARNING] On May 7, 2024 this sequence was replaced by NM_181083.3.
            
            On Jun 3, 2003 this sequence version replaced NM_181083.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY255526.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q42"
     gene            1..5784
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /note="ADP ribosylation factor guanine nucleotide exchange
                     factor 2"
                     /db_xref="GeneID:296380"
                     /db_xref="RGD:631430"
     CDS             1..5376
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /note="Brefeldin A-inhibited guanine nucleotide-exchange
                     factor 2; brefeldin A-inhibited GEP 2; ADP-ribosylation
                     factor guanine nucleotide-exchange factor 2 (brefeldin
                     A-inhibited)"
                     /codon_start=1
                     /product="brefeldin A-inhibited guanine
                     nucleotide-exchange protein 2"
                     /protein_id="NP_851597.2"
                     /db_xref="GeneID:296380"
                     /db_xref="RGD:631430"
                     /translation="MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIK
                     AELEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITG
                     NAPDSGAPGKRLIDRIVETVCNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTIL
                     QTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQS
                     PVIQATAGSPKFSRLKQSQAQSKPTTPEKTELPNGDHARSSLGKVNSENGEAHRERGS
                     SISGRAEPSGGSDNGAQEVVKDILEDVVTSAVKEAAEKQGLPEPDQAPGVPECQECTV
                     PPAVDENSQTNGIADDRQSLSSADNLEPDAQGHPVAARFSHILQKDAFLVFRSLCKLS
                     MKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQYLCVALSKN
                     GVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQ
                     TLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSL
                     RKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTS
                     VESTVSSGTQTAIPDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAV
                     EDIAQFLHQEERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGF
                     RLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNK
                     MTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKEHTMATKSTKQNVASE
                     KQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSI
                     GLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQ
                     KNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREG
                     SLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTR
                     LDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIG
                     DHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSP
                     TIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTI
                     FQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTS
                     DDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWW
                     QDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVF
                     AQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFRTTIPHVLL
                     TWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDDSWKGAPYANQKL
                     LASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETENQGMY
                     KFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLAC
                     CLRILFRMYVDENRRDSWGEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTK
                     TLKINDEKFKAHASVYYPYLCEMMQFDLIPELRAVLRKFFLRIGLVYKIWVPEEPSQV
                     PAASTAW"
     exon            1..121
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            122..152
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            153..276
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            277..423
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            424..603
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            604..859
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            860..928
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1080
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1211
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1446
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1546
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1686
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1795
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1979
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2091
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2297
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            2298..2382
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..2554
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2706
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2835
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            2836..2994
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            2995..3142
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            3143..3242
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            3243..3283
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            3284..3453
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            3454..3605
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            3606..3778
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            3779..3939
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            3940..4070
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            4071..4200
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            4201..4336
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            4337..4475
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            4476..4530
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            4531..4645
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            4646..4776
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            4777..4945
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            4946..5084
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            5085..5202
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
     exon            5203..5767
                     /gene="Arfgef2"
                     /gene_synonym="Big2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgcaggaaa gccagaccaa gagcatgttc gtgtcccggg ccctggagaa gatcctagct
       61 gacaaggagg tgaagcggcc ccagcactcc cagctgcgta gggcctgcca ggtggcgctc
      121 gatgaaatta aagcagaact agaaaagcag aggcttggcg ctgcggctcc accaaaggca
      181 aacttcattg aagctgacaa gtatttcctt ccattcgagc tggcctgcca atccaagtcc
      241 cctcgggtgg tcagcacgtc cctggactgc ttgcagaaac tcatcgcata tgggcacatc
      301 actggcaatg cccccgacag tggtgctcct gggaagcggc tgatcgacag gattgtggag
      361 acggtttgca attgttttca gggccctcag acagatgaag gcgttcagct acagataatt
      421 aaggctcttc tgactgctgt gacttcccca cacattgaaa tccatgaggg caccattcta
      481 cagacagtga ggacatgcta caatatctat ttggccagca aaaacctcat caatcaaacc
      541 accgccaagg ccacgctcac ccaaatgctg aatgtcatct tcacccgcat ggaaaaccaa
      601 gtgttacagg aggccagaga attggaaaaa ccaatccagt caaaacctca gtccccagtg
      661 atccaggcca cagcaggatc tcccaagttc agtcgactga agcagagcca ggcccagagc
      721 aaaccaacaa ctcctgaaaa gacagaattg cccaatggtg accacgccag gagtagccta
      781 ggaaaagtga attcagaaaa tggagaggct caccgagaaa gaggctcatc aatctccggt
      841 agagctgagc cttctggggg atctgacaat ggagcccagg aggtggtgaa ggacatctta
      901 gaggacgtgg tcacgtctgc tgtgaaagaa gcagcagaaa agcaggggct gccggagcct
      961 gaccaagccc caggtgtgcc agagtgccag gaatgcactg ttcctcctgc agttgatgaa
     1021 aactcacaga ccaatggcat agcagatgac aggcagtcgc tgtcctcggc agataacctg
     1081 gaaccagatg ctcaaggaca cccagtggct gctagattct ctcacattct acagaaggat
     1141 gccttcctgg tgtttcgttc cctgtgtaag ctgtccatga agccgcttgg ggaaggacca
     1201 ccagatccaa agtcccacga gcttcgttcc aaggtggttt ccctgcagct gctgctctct
     1261 gtgctgcaga acgctggtcc agtcttcagg agccacgaga tgttcgtcac tgccattaag
     1321 cagtacctgt gtgtagccct ttccaagaac ggcgtgtcct cagtgcctga cgtcttcgaa
     1381 ctctcacttg cgatttttct tactcttctt tcaaacttca agatgcactt gaaaatgcag
     1441 atagaggtct ttttcaaaga gattttcctg aacattttag aaacgtccac aagttctttt
     1501 gagcaccgct ggatggtcat tcagaccctg acgagaattt gtgcagatgc ccagtgtgtt
     1561 gtggatattt atgtcaacta cgactgtgac ttaaatgctg ctaacatttt tgagcgtcta
     1621 gtaaatgatt tatccaaaat cgctcaggga agaagtggac atgaactggg gatgacacct
     1681 ctacaggagc tcagtctgag gaagaaaggc ctggagtgtc tggtgtccat tctcaagtgc
     1741 atggtggaat ggagcaagga cctgtatgtg aatcccaacc accaggccac cctgggtcag
     1801 gaacggcttc cagatcagga aatgggggac ggaaaaggcc ttgacatggc aagacggtgc
     1861 agcgtgacat cagtggagtc cacagtgtcc tcggggaccc agacagccat tccagatgac
     1921 ccagagcagt ttgaggtcat caaacaacag aaggaaatca tcgagcatgg cattgagctc
     1981 ttcaataaga agcccaagcg gggaatccag tttctccaag agcaaggcat gctaggagca
     2041 gctgtagaag atattgccca atttctgcac caggaagagc gtctggattc tacccaggtt
     2101 ggtgagtttc tgggagacag cacaaggttt aacaaggagg tgatgtatgc ctatgtggac
     2161 caactggact tctgtgaaaa agagttcgtc tcggccctga ggacattcct ggagggtttc
     2221 cgcctgcctg gggaagccca gaagattgac cggttaatgg agaagtttgc tgcacgatat
     2281 atagagtgta accaagggca aaccctgttt gctagtgctg acacggcgta cgtcctggcc
     2341 tactccatca ttatgctaac tacagacctg catagccctc aggtaaagaa taaaatgaca
     2401 aaggaacagt atattaaaat gaaccggggc atcaacgata gtaaagacct acctgaggag
     2461 tacctgtcca gcatctacga agagatagaa ggcaagaaga ttgcgatgaa ggagaccaag
     2521 gagcacacaa tggcaacgaa atccaccaag cagaatgtag ccagtgagaa gcagaggcgg
     2581 ctgctgtaca atgtggagat ggagcagatg gctaagacag ccaaagctct gatggaggcc
     2641 gtgagccacg ccaaagcccc gtttaccagc gccacacact tggaccatgt ccggccaatg
     2701 ttcaaactgg tgtggacgcc actgctggca gcctatagca tcggcctaca gaactgtgac
     2761 gacacggagg tggcctccct gtgcttggaa ggcatcaggt gtgcagtccg cattgcctgc
     2821 atctttggaa tgcagctgga acgagatgcc tatgttcagg cccttgctcg cttttccctg
     2881 ctgacagcca gctccagcat cacagaaatg aagcagaaaa acattgacac cataaagaca
     2941 ctcatcactg tggctcacac cgatggcaac taccttggca actcctggca tgagatcttg
     3001 aagtgcatca gccagctgga gctcgctcag ctcataggaa ctggagtgaa gactcgctac
     3061 ctctctggct ctgggcggga aagagaaggg agcctgaagg gccacagtct ggcaggagaa
     3121 gaattcatgg gtcttggcct cggtaatttg gtgagtggtg gtgtggataa aagacagatg
     3181 gccagcttcc aggagtccgt tggtgaaacc agctcacaga gtgtggttgt tgcagttgac
     3241 agaattttta ccggctctac cagactggat ggaaatgcaa tagttgactt tgtccgctgg
     3301 ctgtgtgctg tgtccatgga tgagctggct tctccccacc atccccgcat gttcagcctg
     3361 cagaagatcg tggagatatc atactacaac atgaaccgga tccggctgca gtggtctcgg
     3421 atatggcatg tcattggaga tcacttcaat aaggtcggct gtaaccccaa tgaggatgtg
     3481 gccatctttg ctgttgactc gctgcggcaa ctctccatga agtttttgga gaagggagaa
     3541 ttagccaact tccgatttca gaaggatttt ctgaggccgt ttgagcatat tatgaagaaa
     3601 aacaggtccc caaccatccg ggacatggtc atccgctgca ttgcccagat ggtgagctcc
     3661 caggcagcca acatccgctc aggctggaag aacatctttg ctgtgttcca ccaggccgcc
     3721 tctgaccacg atgggaacat tgtggagctg gccttccaga ccaccggcca cattgtctca
     3781 accatcttcc agcaccactt ccctgcggcc atcgactcct tccaggacgc tgtgaagtgc
     3841 ttgtctgagt ttgcctgcaa cgcagctttc ccggacacca gtatggaggc catccggctc
     3901 atccgcttct gtgggaaata cgtctcagag aggccacggg tactgcagga gtacacaagc
     3961 gatgacatga atgtggctcc tggtgacagg gtgtgggtca gaggctggtt ccccatcctc
     4021 tttgagctct cctgcatcat taacaggtgc aagttagatg tgcgcacaag gggactgaca
     4081 gtcatgttcg agatcatgaa gagctatggc cacacctttg caaagcactg gtggcaggac
     4141 ctattcagaa ttgtgtttcg gatttttgac aacatgaaac tccctgagca acagtcggag
     4201 aaatcagagt ggatgacgac cacctgcaat cacgcactgt atgctatttg tgacgtattc
     4261 acccagttct acgaggcttt gcatgaagtg cttctctccg atgtgtttgc acagctgcag
     4321 tggtgcgtga agcaagataa cgagcagttg gcccgctcgg gtacgaattg cttagagaac
     4381 ctagtgatat ccaatggaga gaagttcagc cctgctgtct gggatgaaac ctgcaattgc
     4441 atgttggata ttttcagaac caccatccca catgttttgc tgacgtggag acctgcgggg
     4501 atggaggagg aggtgtcaga tagacatctg gacgtggacc tggaccgcca gtctttaagc
     4561 agcatagaca gaaacgcctc ggagagagga cagagccagc tgtctaaccc cactgatgac
     4621 agctggaagg gagcaccgta tgcaaatcag aaactgctgg ccagccttct catcaagtgt
     4681 gtggttcagc tggagctgat acagaccatt gacaacatcg tgttctaccc tgcgaccagc
     4741 aaaaaggagg atgcagagca catggttgct gcccagcaag acacactaga tgcagatatc
     4801 cacattgaga cggagaatca gggcatgtac aagttcatgt cttcccagca cctcttcaag
     4861 ctgctagact gcctgcagga gtcccattcc ttctccaagg ccttcaactc caactatgag
     4921 cagcggactg tcctctggcg agctggcttc aagggcaagt ccaaaccaaa tctcctcaaa
     4981 caagaaacca gcagcttggc ctgttgcctg aggatcctgt ttagaatgta cgttgatgag
     5041 aaccgcaggg attcctgggg cgaaatacag cagcggcttt taaccgtgtg cagcgaagca
     5101 cttgcctatt tcattactgt gaactctgag agccatcgag aagcctggac gagcctcctc
     5161 ctcctccttc taaccaaaac cctcaagatc aacgatgaaa agttcaaagc ccacgcgtca
     5221 gtgtactacc cctacctgtg tgagatgatg cagtttgacc tgatcccgga gctccgagca
     5281 gttctgcgaa agttcttcct gcgaataggc ctggtgtata agatatgggt tccagaagag
     5341 ccatcgcaag tgccagcggc atcgacagca tggtagcacc atggccactg ctgtggttct
     5401 gtagaatacc cgccatgccg tcccagctgt ggaacgagga gctgcccgcc atgttggggt
     5461 ttggaactgg agagcagtgg cctgaacact ggccttgtca cagtccccag cagaggctca
     5521 aggtgtcatg tataaccatc acacaccagt aactgggtgg gtggggagtc tgttccaacc
     5581 gtcattccat ttcactgact gaactgtcag atctgtggtg agggccacca tttcctcccg
     5641 acctctgaga ctggccataa atgatttgtc ggaggaagtc actctcctgg gaatcactga
     5701 gcattgctgt ataggtccat gtttattaga gagtattaat aaaattcctt gtaatcgcta
     5761 acagccaaaa aaaaaaaaaa aaaa
//
