LOCUS       NM_001277056            6945 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Rattus norvegicus ARF guanine nucleotide exchange factor 1
            (Arfgef1), mRNA.
ACCESSION   NM_001277056 XM_001066125 XM_232614
VERSION     NM_001277056.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Li,C., Chen,S., Yu,Y., Zhou,C., Wang,Y., Le,K., Li,D., Shao,W.,
            Lu,L., You,Y., Peng,J., Huang,H., Liu,P. and Shen,X.
  TITLE     BIG1, a brefeldin A-inhibited guanine nucleotide-exchange factor,
            is required for GABA-gated Cl influx through regulation of GABAA
            receptor trafficking
  JOURNAL   Mol Neurobiol 49 (2), 808-819 (2014)
   PUBMED   24198228
  REMARK    GeneRIF: A role was identified for BIG1 in modulating GABA-gated
            Cl(-) influx through regulation of GABA A receptor expression.
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Zhou,C., Li,C., Li,D., Wang,Y., Shao,W., You,Y., Peng,J., Zhang,X.,
            Lu,L. and Shen,X.
  TITLE     BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein
            regulates neurite development via PI3K-AKT and ERK signaling
            pathways
  JOURNAL   Neuroscience 254, 361-368 (2013)
   PUBMED   24090963
  REMARK    GeneRIF: Overexpression of BIG1 in neurites significantly increases
            ERK phosphorylation.
REFERENCE   3  (bases 1 to 6945)
  AUTHORS   Lin,S., Zhou,C., Neufeld,E., Wang,Y.H., Xu,S.W., Lu,L., Wang,Y.,
            Liu,Z.P., Li,D., Li,C., Chen,S., Le,K., Huang,H., Liu,P., Moss,J.,
            Vaughan,M. and Shen,X.
  TITLE     BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein
            modulates ATP-binding cassette transporter A-1 trafficking and
            function
  JOURNAL   Arterioscler Thromb Vasc Biol 33 (2), e31-e38 (2013)
   PUBMED   23220274
  REMARK    GeneRIF: BIG1, through its ability to activate ADP-ribosylation
            factor 1, regulates cell-surface levels and function of ABCA1.
REFERENCE   4  (bases 1 to 6945)
  AUTHORS   Li,C.C., Kuo,J.C., Waterman,C.M., Kiyama,R., Moss,J. and Vaughan,M.
  TITLE     Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG)
            1 and KANK1 proteins on cell polarity and directed migration during
            wound healing
  JOURNAL   Proc Natl Acad Sci U S A 108 (48), 19228-19233 (2011)
   PUBMED   22084092
REFERENCE   5  (bases 1 to 6945)
  AUTHORS   Boal,F. and Stephens,D.J.
  TITLE     Specific functions of BIG1 and BIG2 in endomembrane organization
  JOURNAL   PLoS One 5 (3), e9898 (2010)
   PUBMED   20360857
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6945)
  AUTHORS   Saeki,N., Tokuo,H. and Ikebe,M.
  TITLE     BIG1 is a binding partner of myosin IXb and regulates its
            Rho-GTPase activating protein activity
  JOURNAL   J Biol Chem 280 (11), 10128-10134 (2005)
   PUBMED   15644318
  REMARK    GeneRIF: myosin IXb binds to BIG1, which regulates its Rho-GTPase
            activating protein activity [BIG1]
REFERENCE   7  (bases 1 to 6945)
  AUTHORS   Padilla,P.I., Pacheco-Rodriguez,G., Moss,J. and Vaughan,M.
  TITLE     Nuclear localization and molecular partners of BIG1, a brefeldin
            A-inhibited guanine nucleotide-exchange protein for
            ADP-ribosylation factors
  JOURNAL   Proc Natl Acad Sci U S A 101 (9), 2752-2757 (2004)
   PUBMED   14973189
REFERENCE   8  (bases 1 to 6945)
  AUTHORS   Li,H., Adamik,R., Pacheco-Rodriguez,G., Moss,J. and Vaughan,M.
  TITLE     Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited
            guanine nucleotide-exchange protein 2 (BIG2)
  JOURNAL   Proc Natl Acad Sci U S A 100 (4), 1627-1632 (2003)
   PUBMED   12571360
REFERENCE   9  (bases 1 to 6945)
  AUTHORS   Zhao,X., Lasell,T.K. and Melancon,P.
  TITLE     Localization of large ADP-ribosylation factor-guanine nucleotide
            exchange factors to different Golgi compartments: evidence for
            distinct functions in protein traffic
  JOURNAL   Mol Biol Cell 13 (1), 119-133 (2002)
   PUBMED   11809827
REFERENCE   10 (bases 1 to 6945)
  AUTHORS   Yamaji,R., Adamik,R., Takeda,K., Togawa,A., Pacheco-Rodriguez,G.,
            Ferrans,V.J., Moss,J. and Vaughan,M.
  TITLE     Identification and localization of two brefeldin A-inhibited
            guanine nucleotide-exchange proteins for ADP-ribosylation factors
            in a macromolecular complex
  JOURNAL   Proc Natl Acad Sci U S A 97 (6), 2567-2572 (2000)
   PUBMED   10716990
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On or before Mar 2, 2013 this sequence version replaced
            XM_001066125.3, XM_232614.6.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643297.18084.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-280               JAXUCZ010000005.1  13764975-13765254
            281-311             JAXUCZ010000005.1  13781281-13781311
            312-468             JAXUCZ010000005.1  13781906-13782062
            469-615             JAXUCZ010000005.1  13783233-13783379
            616-795             JAXUCZ010000005.1  13784245-13784424
            796-1072            JAXUCZ010000005.1  13787732-13788008
            1073-1174           JAXUCZ010000005.1  13792100-13792201
            1175-1350           JAXUCZ010000005.1  13796902-13797077
            1351-1484           JAXUCZ010000005.1  13797794-13797927
            1485-1719           JAXUCZ010000005.1  13802341-13802575
            1720-1819           JAXUCZ010000005.1  13806871-13806970
            1820-1959           JAXUCZ010000005.1  13807044-13807183
            1960-2068           JAXUCZ010000005.1  13807279-13807387
            2069-2270           JAXUCZ010000005.1  13807645-13807846
            2271-2382           JAXUCZ010000005.1  13813932-13814043
            2383-2588           JAXUCZ010000005.1  13814217-13814422
            2589-2673           JAXUCZ010000005.1  13814650-13814734
            2674-2845           JAXUCZ010000005.1  13815386-13815557
            2846-2997           JAXUCZ010000005.1  13816706-13816857
            2998-3126           JAXUCZ010000005.1  13821949-13822077
            3127-3285           JAXUCZ010000005.1  13822778-13822936
            3286-3436           JAXUCZ010000005.1  13823146-13823296
            3437-3527           JAXUCZ010000005.1  13831592-13831682
            3528-3568           JAXUCZ010000005.1  13836029-13836069
            3569-3738           JAXUCZ010000005.1  13836164-13836333
            3739-3890           JAXUCZ010000005.1  13837053-13837204
            3891-4063           JAXUCZ010000005.1  13837331-13837503
            4064-4224           JAXUCZ010000005.1  13839040-13839200
            4225-4355           JAXUCZ010000005.1  13840017-13840147
            4356-4485           JAXUCZ010000005.1  13842535-13842664
            4486-4621           JAXUCZ010000005.1  13843189-13843324
            4622-4760           JAXUCZ010000005.1  13843411-13843549
            4761-4833           JAXUCZ010000005.1  13844968-13845040
            4834-4966           JAXUCZ010000005.1  13849129-13849261
            4967-5106           JAXUCZ010000005.1  13851786-13851925
            5107-5275           JAXUCZ010000005.1  13854118-13854286
            5276-5414           JAXUCZ010000005.1  13855127-13855265
            5415-5532           JAXUCZ010000005.1  13856223-13856340
            5533-6945           JAXUCZ010000005.1  13857825-13859237
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q11"
     gene            1..6945
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /note="ARF guanine nucleotide exchange factor 1"
                     /db_xref="GeneID:312915"
                     /db_xref="RGD:1560793"
     exon            1..280
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..5697
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /note="brefeldin A-inhibited GEP 1; ADP-ribosylation
                     factor guanine nucleotide-exchange factor 1(brefeldin
                     A-inhibited); ADP ribosylation factor guanine nucleotide
                     exchange factor 1"
                     /codon_start=1
                     /product="brefeldin A-inhibited guanine
                     nucleotide-exchange protein 1"
                     /protein_id="NP_001263985.1"
                     /db_xref="GeneID:312915"
                     /db_xref="RGD:1560793"
                     /translation="MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEI
                     KVETEKQSPPHGEAKAGSGTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDC
                     LQKLIAYGHLTGSAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVT
                     SQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAK
                     QMERERHRQQHHLLQSPVSHHEPESPHLRYLPPQTVDHIAQEQEGDLDPQTHDVDKSL
                     QDDIEPENGSDISSAENEQTEADQATAAETLSKDDVLCDGECEEKPQDIVQSIVEEMV
                     DIIVGDMGEGTAVSASADGNAGAVEDGSDSENVQANGIPGTPISAAYTPSLPDDRLSV
                     SSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSK
                     ILSLQLLLSILQNAGPVFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTL
                     LSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNY
                     DCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWS
                     KDQYVNPNSQTTLGQEKPSEQEISEIKHPETINRYGSLNSLESTSSSGIGSYSTQMSG
                     TDNPEQFEVLKQQKEIIEQGIDLFNKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERL
                     DSTQAGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRLFLEGFRLPGEAQKIDRLM
                     EKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGI
                     NDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPTKSTKQNVASEKQRRLLYNLEMEQ
                     MAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTDVASL
                     CLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVA
                     HTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGTKDQAPDEF
                     VGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCA
                     VSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVA
                     IFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVN
                     SQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTSGHIVTLVFEKHFPATIDSFQDA
                     VKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRG
                     WFPILFELSCVINRCKLDVRTRGLTVMFEIMKTYGHTYEKHWWQDLFRIVFRIFDNMK
                     LPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLA
                     RSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPTSGEAAPPSPS
                     AMSEKQLDAISQKSVDIHDSAQPRSSDNRQQAPLVSVSPASEEVSKGRPTAKFPEQKL
                     FAALLIKCVVQLELIQTIDNIVFFPATSKKEDAENLAAAQRDAVDFDVRVDTQDQGMY
                     RFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLAC
                     GLRILFRMYTDESRVSAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTK
                     VLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGIVFQISQPPEQELG
                     INKQ"
     exon            281..311
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            312..468
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            469..615
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            616..795
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            796..1072
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1174
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1350
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1484
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1719
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..1819
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1959
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2068
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2270
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            2271..2382
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..2588
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            2589..2673
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            2674..2845
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..2997
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            2998..3126
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            3127..3285
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            3286..3436
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            3437..3527
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            3528..3568
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            3569..3738
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            3739..3890
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            3891..4063
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            4064..4224
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            4225..4355
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            4356..4485
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            4486..4621
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            4622..4760
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            4761..4833
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            4834..4966
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            4967..5106
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            5107..5275
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            5276..5414
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            5415..5532
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
     exon            5533..6945
                     /gene="Arfgef1"
                     /gene_synonym="BIG1; RGD1560793"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggccgctc tgcgtcgccc gccgcttcct ggtggcgcgg ctgccctcca cgcctcctcg
       61 cccccatcgc cacctcctcg cctttctccc tccccgtctc cctcctcagc ctcccctcca
      121 gccgccggga ctgccgccgc ctcctcctcc gcgtccatgt atgagggcaa gaagacgaag
      181 aacatgttcc tgacccgggc cctggagaag attttggccg acaaggaagt gaagaaggcg
      241 catcactccc agctgcgcaa agcgtgcgag gtggccctag aggaaataaa agttgaaact
      301 gagaaacaaa gtcctcctca tggagaagca aaagctggat caggcaccct tccaccagtg
      361 aaatcaaaga caaattttat tgaagcagac aagtacttct taccctttga gttggcatgc
      421 cagtccaaat gtccccgcat agttagtaca tctctagact gtttgcagaa acttattgct
      481 tatgggcact tgactggcag tgctccagat agtacgacac caggcaaaaa gttaattgat
      541 agaattattg aaacaatatg tggctgcttc caaggccctc aaacagatga aggagttcag
      601 ttgcagataa taaaggcttt acttactgca gtaacatcac aacacataga aattcacgaa
      661 ggaactgtgc tgcaagctgt gaggacatgc tacaatatct atttagccag caaaaatctc
      721 atcaatcaga caacagccaa agctactctt actcagatgc taaatgttat ctttgcacgc
      781 atggaaaacc aagcattaca ggaagccaaa caaatggaaa gagaaagaca tcggcagcag
      841 catcacttgt tacagtctcc agtaagccat cacgagcccg agtcacctca tcttagatat
      901 ttgccacctc agactgttga tcatatagcc caagaacagg aaggggatct tgatccccaa
      961 acacacgatg tggataaaag tctccaagat gacatagagc ctgagaacgg atctgatatt
     1021 tccagtgcag agaatgaaca gacagaagct gatcaggcaa ctgcagctga aacattatct
     1081 aaagatgatg tgttgtgtga tggagagtgt gaggaaaagc cacaggatat tgtgcagagc
     1141 attgtggaag agatggtgga catcattgtt ggagacatgg gggaaggaac agctgtaagt
     1201 gcaagtgcag atggcaacgc tggagctgta gaggatggca gtgacagtga gaacgtgcaa
     1261 gccaatggga tcccgggaac acccatctct gccgcataca caccatcgtt acctgacgac
     1321 agattgtctg tctcctccaa tgatactcag gaatctggaa attcttcagg accttcacct
     1381 ggtgctaagt tttcccacat tttacaaaag gatgcctttc tagtgttcag gtcattgtgt
     1441 aagctgtcaa tgaaaccact gtcagatgga ccaccagatc caaagtctca tgagttacgg
     1501 tccaagattc tttcattgca gttgcttctg tccattctgc agaatgcagg acctgttttc
     1561 aggacaaatg agatgtttat taatgctatc aagcagtatc tgtgtgttgc actctcaaaa
     1621 aacggagtct catctgttcc agaggttttt gagctttctc tttccatatt tctaactttg
     1681 ttgtcaaact tcaagacaca tctgaagatg caaattgagg tattctttaa agaaattttc
     1741 ctgtacattt tggaaacttc tacaagctca tttgatcata agtggatggt gattcagaca
     1801 ctgacaagga tctgtgcaga tgctcagagt gtggtggata tttatgtaaa ttatgactgt
     1861 gacttgaatg cagcaaatat atttgaaaga ctagtaaatg acctgtcaaa aattgcccaa
     1921 ggaaggggca gtcaagaact tgggatgagt aatgttcagg aattgagcct gcggaaaaag
     1981 gggttagaat gtttagtttc aattcttaag tgtatggttg aatggagtaa ggatcagtac
     2041 gtgaatccca actctcaaac cacccttggt caggaaaaac cctcagagca agagataagt
     2101 gaaatcaaac accccgagac tataaacaga tatggaagtt taaactcctt agaatccaca
     2161 tcatcttcag ggattggcag ctacagcaca cagatgtctg gcactgataa tccagaacaa
     2221 ttcgaggtcc taaagcagca aaaagaaata atagaacaag ggattgattt atttaataag
     2281 aaaccaaaga gaggaataca gtacctccaa gaacaaggga tgcttggcac cactcctgaa
     2341 gatatagctc agttcttaca tcaagaagaa agattagact ctacacaggc gggggagttc
     2401 ctgggagata acgataagtt caataaggaa gtcatgtatg catatgtaga ccagcatgac
     2461 ttttcgggaa aagactttgt ttcagctctt cgtctgtttc tggaaggatt ccggcttcca
     2521 ggagaagctc agaaaattga tcgattaatg gaaaaatttg ctgcaagata cctagagtgc
     2581 aaccaaggac aaaccctttt tgctagtgca gatactgctt atgttttggc ctactcaatt
     2641 atcatgttga ccacagacct tcacagtcca caggttaaaa acaaaatgac gaaagaacag
     2701 tatattaaaa tgaatcgtgg tatcaatgac agtaaagatc tccctgaaga gtatctatct
     2761 gccatttata atgagatagc tgggaaaaaa atatcaatga aagaaacaaa agaactaaca
     2821 atccctacaa aatcaactaa acagaatgta gccagtgaaa agcaaagacg acttctgtat
     2881 aatttagaaa tggaacagat ggccaagaca gctaaagcac tcatggaagc tgtgagccat
     2941 gttcaggcac cttttacaag tgcaacacac ttggagcacg tgaggcctat gtttaagttg
     3001 gcttggaccc cttttctggc tgccttcagt gtgggtctac aagactgtga tgatactgac
     3061 gtggcctctc tctgcctcga agggatacga tgtgcgatca gaattgcatg catcttcagc
     3121 attcagctgg aaagagatgc atatgtccag gcactagcca gatttacctt acttacagtg
     3181 agttctggta ttactgaaat gaaacagaag aatatcgaca caataaaaac actcatcaca
     3241 gtggctcaca cagatggaaa ttatttagga aattcgtggc atgagatcct gaagtgcatt
     3301 agtcagttag agcttgcaca acttatagga actggagtga agcctcggta catttctggg
     3361 accgtgagag gcagagaagg gtctctcact gggacaaaag atcaggctcc ggatgaattt
     3421 gttggtctgg ggctagttgg aggaaatgtg gactggaagc agatagccag tattcaggag
     3481 tctattggag aaaccagttc tcaaagtgtc gtggttgctg tagacagaat attcacagga
     3541 tccacaaggc tagatggaaa tgctattgtc gattttgttc gttggctctg tgccgtgtct
     3601 atggatgaat tgctttccac tacacaccca agaatgttta gtctacaaaa aatagtagaa
     3661 atatcatact ataacatggg aagaataaga ctgcagtggt ctcggatttg ggaagttatt
     3721 ggagatcatt ttaataaggt tggttgcaat cctaatgaag atgtagccat atttgcagta
     3781 gactccctga ggcagctatc gatgaagttt ttagagaaag gagagcttgc aaacttcaga
     3841 ttccaaaagg atttcttaag accttttgaa catataatga aacgaaacag gtctccaaca
     3901 attcgagata tggtcgtgcg ttgtatagcg cagatggtta actctcaggc tgccaatatc
     3961 cgttcaggat ggaagaacat tttctcggtg ttccacctag ctgcatcaga ccaggatgag
     4021 agcatagtag agctagcatt ccagacgagc gggcacatcg tcactcttgt atttgaaaag
     4081 cactttccag ccactattga ttctttccag gatgcagtga agtgtttatc tgaatttgcg
     4141 tgtaatgcgg ctttcccaga cacaagtatg gaagcaatcc gacttattcg ccattgtgca
     4201 aaatatgtgt ctgatagacc tcaggctttc aaagagtaca caagtgatga catgaacgtg
     4261 gcacctgaag acagggtgtg ggtgagaggg tggttcccaa tcctctttga gttgtcctgt
     4321 gtcatcaata gatgcaaatt agatgtaaga accaggggct taacagtgat gtttgaaata
     4381 atgaaaacat acggccacac ttacgaaaaa cactggtggc aggacttgtt cagaattgtt
     4441 ttcagaatct ttgacaacat gaagttgcca gaacagcaga cagagaaagc tgaatggatg
     4501 acaacaactt gtaaccatgc cctttatgcg atatgcgacg tgttcaccca gtatttagaa
     4561 gtcctcagtg atgtactttt ggatgatatt tttgcccagc tttactggtg tgtacaacaa
     4621 gacaatgagc agttagcacg atctggtacg aactgcttag aaaatgtggt tattctgaat
     4681 ggtgagaagt ttactctaga aatctgggat aaaacctgta attgcaccct ggatatcttc
     4741 aaaaccacaa ttcctcatgc gcttctgact tggcgtccta cttctggaga ggctgccccc
     4801 ccatctccat cagctatgag tgaaaagcag ttggatgcaa tatcacagaa atctgtagac
     4861 atacatgatt ctgctcaacc aagatcttca gataacaggc agcaagcacc acttgtttct
     4921 gtttctcctg cgagtgaaga agttagcaaa gggagaccca cagcaaaatt cccagaacaa
     4981 aaattgtttg ctgccctgtt gattaaatgt gttgtgcagc tggaactcat ccagaccatc
     5041 gacaacattg tgttcttccc agccacaagc aagaaggaag atgcagaaaa cttagctgca
     5101 gcccagagag atgcagtgga cttcgatgtt cgagttgata ctcaagacca aggaatgtac
     5161 cgctttttaa catcacaaca actcttcaag ctactggact gcttattaga gtcacataga
     5221 tttgcaaaag catttaattc caacaatgaa cagaggactg ctctatggaa agcaggcttc
     5281 aaaggcaagt ccaagcccaa tctcctaaaa caagagacaa gcagcctggc gtgtggactg
     5341 cgcatcctct tccggatgta cacagacgag agcagagtca gcgcctggga ggaggtccag
     5401 cagaggcttt taaacgtctg cagtgaggca ctgagttact tcctcactct aacatctgaa
     5461 agtcaccggg aagcctggac caacttactg ttactgttcc tcactaaagt cctcaagatc
     5521 agtgacaaca ggtttaaagc tcacgcatca ttctactacc ctctgttatg tgaaattatg
     5581 caatttgact tgattcctga acttcgtgct gttcttagaa gatttttcct gcgaattgga
     5641 atagtatttc agatatcaca accacctgaa caggaacttg ggataaacaa gcaatgatga
     5701 gaaccgaatg tttttctgtt ggtgtttaca gtcctctgct ccgtgagagg cccctggagc
     5761 agaggtctcc tacctgaaaa ctgtttctct gaagtacagt gtctgagagt tactgataag
     5821 atgcttatac cggtagctca aggtcggaac actccatcag tcactttagt gacccagtgg
     5881 ctgctgtctg ctgttgggct catgttgagc agaaaggctg ttcagacagc atcagcctac
     5941 cttcgggtcc taccctgtcc tctgagcagg gtgagcccat actttggcag gtacggaaat
     6001 gaaacttacc caaagaagta tagatgtaaa gactgaactt ctgcaagaag tacaactcag
     6061 gagagctgta ttttgaagga taaaatgttt ataattgggg ggaagggaga aaaaaattat
     6121 tgctcatggt aaaagatatt tagcgactat ggtgttctta ctctgaagat ttttgcacac
     6181 tcaggctatc tgagatactg gtaatcatca tctttctgtg aaaatgtaca gagatgcagg
     6241 tctgtaatat aaaaatctta aacatattgt tcttcctgca ctgttttatt tctttatttt
     6301 cttatctact tgctaaatac ccataatatt ttgtcaaatg cactaaaaca tttgggttga
     6361 acttttagtc tttgggggag tggtggtcat tttgaggact tagtatatgc cctgaatctg
     6421 ttgtggccaa tgtttgtcaa gatcacaaca gaagggggag ccaccaaggt agaaaacatc
     6481 cctgctgaca agaactacct gtaacatact ctcttagggc ttttaacgat gagccattga
     6541 aatagatgac ctttcacgac tgaaacttga atcactgcga atgaatctag gttgctgtca
     6601 ctttttcttt tgggtggcgg ggcttgatgt agattttatt ctatgtacag aatttaatgt
     6661 tgaatatatt aaaataacca atctggcatg gtttggggcg gttagattta ctggaaatgc
     6721 attcatactg tgaattgtgc tctgatggtt aacgaaaagc ttgtcaagca ttccatgttg
     6781 acggtggggt aaagaggttc caggtggatg tgtatatggt acagttgtaa agttttctat
     6841 gtaacattct gtacaacttt ctgtacaata tggattctca tctggcatat ctaatcaggt
     6901 tataggtcaa taaagttttt gaattatttc atcagtgcag tgtaa
//
