LOCUS       NM_022532               2350 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus A-Raf proto-oncogene, serine/threonine kinase
            (Araf), transcript variant 1, mRNA.
ACCESSION   NM_022532
VERSION     NM_022532.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Huang,L.E., Cohen,A.L., Colman,H., Jensen,R.L., Fults,D.W. and
            Couldwell,W.T.
  TITLE     IGFBP2 expression predicts IDH-mutant glioma patient survival
  JOURNAL   Oncotarget 8 (1), 191-202 (2017)
   PUBMED   27852048
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Gan,X., Wang,J., Wang,C., Sommer,E., Kozasa,T., Srinivasula,S.,
            Alessi,D., Offermanns,S., Simon,M.I. and Wu,D.
  TITLE     PRR5L degradation promotes mTORC2-mediated PKC-delta
            phosphorylation and cell migration downstream of Galpha12
  JOURNAL   Nat Cell Biol 14 (7), 686-696 (2012)
   PUBMED   22609986
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2350)
  AUTHORS   Roskoski,R. Jr.
  TITLE     MEK1/2 dual-specificity protein kinases: structure and regulation
  JOURNAL   Biochem Biophys Res Commun 417 (1), 5-10 (2012)
   PUBMED   22177953
  REMARK    Review article
REFERENCE   4  (bases 1 to 2350)
  AUTHORS   Polzien,L., Baljuls,A., Rennefahrt,U.E.E., Fischer,A., Schmitz,W.,
            Zahedi,R.P., Sickmann,A., Metz,R., Albert,S., Benz,R., Hekman,M.
            and Rapp,U.R.
  TITLE     Identification of novel in vivo phosphorylation sites of the human
            proapoptotic protein BAD: pore-forming activity of BAD is regulated
            by phosphorylation
  JOURNAL   J Biol Chem 284 (41), 28004-28020 (2009)
   PUBMED   19667065
REFERENCE   5  (bases 1 to 2350)
  AUTHORS   Yokoyama,T., Takano,K., Yoshida,A., Katada,F., Sun,P., Takenawa,T.,
            Andoh,T. and Endo,T.
  TITLE     DA-Raf1, a competent intrinsic dominant-negative antagonist of the
            Ras-ERK pathway, is required for myogenic differentiation
  JOURNAL   J Cell Biol 177 (5), 781-793 (2007)
   PUBMED   17535970
REFERENCE   6  (bases 1 to 2350)
  AUTHORS   Gruppuso,P.A. and Boylan,J.M.
  TITLE     Hepatic epidermal growth factor-regulated mitogen-activated protein
            kinase kinase kinase activity in the rat: lack of identity with
            known forms of raf and MEKK
  JOURNAL   FEBS Lett 466 (1), 200-204 (2000)
   PUBMED   10648842
REFERENCE   7  (bases 1 to 2350)
  AUTHORS   Kim,S.O., Irwin,P., Katz,S. and Pelech,S.L.
  TITLE     Expression of mitogen-activated protein kinase pathways during
            postnatal development of rat heart
  JOURNAL   J Cell Biochem 71 (2), 286-301 (1998)
   PUBMED   9779826
REFERENCE   8  (bases 1 to 2350)
  AUTHORS   Ishikawa,F., Takaku,F., Nagao,M. and Sugimura,T.
  TITLE     The complete primary structure of the rat A-raf cDNA coding region:
            conservation of the putative regulatory regions present in rat
            c-raf
  JOURNAL   Oncogene Res 1 (3), 243-253 (1987)
   PUBMED   3449797
REFERENCE   9  (bases 1 to 2350)
  AUTHORS   Huleihel,M., Goldsborough,M., Cleveland,J., Gunnell,M., Bonner,T.
            and Rapp,U.R.
  TITLE     Characterization of murine A-raf, a new oncogene related to the
            v-raf oncogene
  JOURNAL   Mol Cell Biol 6 (7), 2655-2662 (1986)
   PUBMED   3491291
REFERENCE   10 (bases 1 to 2350)
  AUTHORS   Williams,A.F., Barclay,A.N., Letarte-Muirhead,M. and Morris,R.J.
  TITLE     Rat thy-1 antigens from thymus and brain: their tissue
            distribution, purification, and chemical composition
  JOURNAL   Cold Spring Harb Symp Quant Biol 41 Pt 1, 51-61 (1977)
   PUBMED   70317
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CO572305.1, X06942.1 and AI712552.1.
            
            On Sep 20, 2005 this sequence version replaced NM_022532.1.
            
            Sequence Note: This RefSeq record was created using sequences from
            two different strains, F344 and Sprague-Dawley, because no single
            transcript from the same strain was available for the full length
            of the gene.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X06942.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                CO572305.1         1-42
            43-2321             X06942.1           10-2288
            2322-2350           AI712552.1         1-29                c
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq11"
     gene            1..2350
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /note="A-Raf proto-oncogene, serine/threonine kinase"
                     /db_xref="GeneID:64363"
                     /db_xref="RGD:2148"
     exon            1..50
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            51..206
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..1925
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; v-raf
                     murine sarcoma 3611 viral oncogene homolog 1; v-raf
                     oncogene homolog 1 (murine sarcoma 3611 virus); Raf
                     related protein; A-Raf proto-oncogene
                     serine/threonine-protein kinase; Ras-binding protein;
                     serine/threonine-protein kinase A-Raf; proto-oncogene
                     A-Raf; proto-oncogene A-Raf-1"
                     /codon_start=1
                     /product="serine/threonine-protein kinase A-Raf isoform 1"
                     /protein_id="NP_071977.1"
                     /db_xref="GeneID:64363"
                     /db_xref="RGD:2148"
                     /translation="MEPPRGPPASGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDS
                     LDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFV
                     RKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRRQFYHSIQD
                     LSGGSRQQEVPSNLSVNELLTPQGPSPFTQQRDQEHFSFPAPANPPLQRIRSTSTPNV
                     HMVSTTAPMDSSLMQFTAQSFSTDAAGRGGDGAPRGSPSPASVSSGRKSPHSKLPAEQ
                     RERKSLADEKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVK
                     VLKVAQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHL
                     HVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLAT
                     VKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYS
                     HIGSRDQIIFMVGRGYLSPDLSKIFSNCPKAMRRLLTDCLKFQREERPLFPQILATIE
                     LLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP"
     exon            207..310
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            311..413
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            414..568
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            569..667
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            668..809
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            810..837
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            838..977
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1180
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1357
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1404
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1523
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1655
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1790
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..2333
                     /gene="Araf"
                     /gene_synonym="Araf1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aagaagacaa gcccaagatg gaggcggtgg tggcggtagc ggtgtgacag gaggcctatg
       61 gcacctgccc agtcctacct cagcccatct tgaaaaaatc cttagacaac atggaaccac
      121 cacgaggccc tcctgctagt ggggctgagc catctcgggc agttggcact gtcaaagtgt
      181 acctgcctaa caagcaacgc acagtggtga ctgtccggga tggcatgagt gtctatgact
      241 ctttggacaa ggccctcaag gtgcggggtc tcaatcagga ctgctgtgtg gtctacagac
      301 tcatcaaagg aagaaagaca gtcactgcct gggacacagc cattgcacct ctggatggcg
      361 aggagctcat tgtggaggtc ctggaagatg tgccactgac catgcacaat tttgtacgga
      421 agacattctt cagtttggcc ttctgtgact tctgccttaa gtttctgttc cacggctttc
      481 gctgccaaac ctgtggctac aagttccacc agcattgttc ctccaaggtc cccacggtct
      541 gcgttgacat gagtaccaac cgccgacagt tctaccacag catccaggat ttgtctggag
      601 gctccaggca gcaggaggtt ccctcaaatc tctctgtgaa tgagctgcta accccccagg
      661 gtcccagtcc ctttacccag caacgtgacc aggagcactt ctccttccct gcccctgcca
      721 atcccccact gcagcgcatc cgctccacat ctactcctaa cgtccacatg gtcagcacca
      781 cagctcccat ggactccagc ctcatgcagt ttactgctca gagcttcagc accgatgctg
      841 ctggtagagg tggtgatgga gctcctcggg gtagccctag cccagctagt gtgtcttcag
      901 ggaggaagtc cccacattcc aagttacctg cagaacagcg ggaacggaag tccttggcag
      961 atgaaaagaa aaaagtgaag aacctggggt accgggactc aggctattac tgggaggtgc
     1021 cacccagtga ggtacagctg ttgaagagga tcgggacagg ctcttttggc actgtgtttc
     1081 gggggcgttg gcatggcgat gtagctgtga aagtgctcaa ggtggcccaa cctaccgctg
     1141 agcaggccca ggccttcaag aatgagatgc aggtgctcag gaagacacga catgtcaaca
     1201 ttttgctgtt tatgggtttc atgactcggc cagggtttgc catcatcaca cagtggtgtg
     1261 agggttccag cctctaccac cacctacatg tggctgacac gcgctttgac atggtccagc
     1321 tcattgatgt ggcccggcag actgcccagg gcatggacta cctccacgcc aagaacatca
     1381 ttcaccgaga cctaaagtcc aacaatatct tcctacatga ggggctcaca gtcaagattg
     1441 gtgacttcgg cctggccaca gtgaagacac gatggagtgg ggcccagccc ttagagcagc
     1501 cctcagggtc tgtgctgtgg atggcagctg aggtgatccg aatgcaggac ccgaacccct
     1561 acagcttcca gtcggatgtc tatgcctatg gtgttgtgct ctatgagctt atgaccggct
     1621 cactgcccta cagccacatt ggcagccgtg accagatcat ctttatggtg ggtcgtggct
     1681 atctgtctcc ggacctcagc aaaatcttca gtaattgccc caaagccatg aggcgcttgc
     1741 tgactgactg cctcaagttc cagcgggagg agcggcctct atttccccag attctggcca
     1801 cgatcgagct gctgcagcgg tcactcccca agattgagcg gagtgcctcc gaaccctcct
     1861 tgcaccgtac ccaggctgat gagttgcctg cctgccttct cagcgcagcc cgccttgtgc
     1921 cttagactcc actcccagcc cactagggag ccattttcag cttaccatgc caaggcaccc
     1981 ccttcctacc agccaatcat tgttctgtct gtgccctgat actgcctcag gattccctat
     2041 cccacaccct gggaaatttg ggggactcca aaaactgagg tcccctgctt cctccataat
     2101 ttggtctcct cttggctttg gggatagttc taatttggag agctgtttta cctccaatgg
     2161 ctgggattca gtgcaaagat tccactcgga acctctttat aaagttttcg cctgacatgt
     2221 cttcactgaa ttatggggtt cccagcaccc catgcggatt tgggagtttc cctttgtctc
     2281 cccccactat tcaaggactc tcctctttac caagaagcac agaattctgc tggaaaaaaa
     2341 aaaaaaaaaa
//
