LOCUS       NM_053837               1976 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Rattus norvegicus adaptor related protein complex 2 subunit mu 1
            (Ap2m1), mRNA.
ACCESSION   NM_053837
VERSION     NM_053837.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Taoufiq,Z., Ninov,M., Villar-Briones,A., Wang,H.Y., Sasaki,T.,
            Roy,M.C., Beauchain,F., Mori,Y., Yoshida,T., Takamori,S., Jahn,R.
            and Takahashi,T.
  TITLE     Hidden proteome of synaptic vesicles in the mammalian brain
  JOURNAL   Proc Natl Acad Sci U S A 117 (52), 33586-33596 (2020)
   PUBMED   33376223
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Helbig,I., Lopez-Hernandez,T., Shor,O., Galer,P., Ganesan,S.,
            Pendziwiat,M., Rademacher,A., Ellis,C.A., Humpfer,N., Schwarz,N.,
            Seiffert,S., Peeden,J., Shen,J., Sterbova,K., Hammer,T.B.,
            Moller,R.S., Shinde,D.N., Tang,S., Smith,L., Poduri,A., Krause,R.,
            Benninger,F., Helbig,K.L., Haucke,V. and Weber,Y.G.
  CONSRTM   EuroEPINOMICS-RES Consortium; GRIN Consortium
  TITLE     A Recurrent Missense Variant in AP2M1 Impairs Clathrin-Mediated
            Endocytosis and Causes Developmental and Epileptic Encephalopathy
  JOURNAL   Am J Hum Genet 104 (6), 1060-1072 (2019)
   PUBMED   31104773
REFERENCE   3  (bases 1 to 1976)
  AUTHORS   Kyung,J.W., Cho,I.H., Lee,S., Song,W.K., Ryan,T.A., Hoppa,M.B. and
            Kim,S.H.
  TITLE     Adaptor Protein 2 (AP-2) complex is essential for functional
            axogenesis in hippocampal neurons
  JOURNAL   Sci Rep 7, 41620 (2017)
   PUBMED   28139716
  REMARK    GeneRIF: Our data reveal that AP-2 has important roles in
            functional axogenesis by proper extension of axon as well as the
            formation of AIS during the early step of neurodevelopment.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1976)
  AUTHORS   De Franceschi,N., Arjonen,A., Elkhatib,N., Denessiouk,K.,
            Wrobel,A.G., Wilson,T.A., Pouwels,J., Montagnac,G., Owen,D.J. and
            Ivaska,J.
  TITLE     Selective integrin endocytosis is driven by interactions between
            the integrin alpha-chain and AP2
  JOURNAL   Nat Struct Mol Biol 23 (2), 172-179 (2016)
   PUBMED   26779610
  REMARK    Erratum:[Nat Struct Mol Biol. 2017 Feb 6;24(2):194. doi:
            10.1038/nsmb0217-194b.. PMID: 28169997]
REFERENCE   5  (bases 1 to 1976)
  AUTHORS   Miller,S.E., Mathiasen,S., Bright,N.A., Pierre,F., Kelly,B.T.,
            Kladt,N., Schauss,A., Merrifield,C.J., Stamou,D., Honing,S. and
            Owen,D.J.
  TITLE     CALM regulates clathrin-coated vesicle size and maturation by
            directly sensing and driving membrane curvature
  JOURNAL   Dev Cell 33 (2), 163-175 (2015)
   PUBMED   25898166
REFERENCE   6  (bases 1 to 1976)
  AUTHORS   Stephens,D.J., Crump,C.M., Clarke,A.R. and Banting,G.
  TITLE     Serine 331 and tyrosine 333 are both involved in the interaction
            between the cytosolic domain of TGN38 and the mu2 subunit of the
            AP2 clathrin adaptor complex
  JOURNAL   J Biol Chem 272 (22), 14104-14109 (1997)
   PUBMED   9162036
REFERENCE   7  (bases 1 to 1976)
  AUTHORS   Boll,W., Ohno,H., Songyang,Z., Rapoport,I., Cantley,L.C.,
            Bonifacino,J.S. and Kirchhausen,T.
  TITLE     Sequence requirements for the recognition of tyrosine-based
            endocytic signals by clathrin AP-2 complexes
  JOURNAL   EMBO J 15 (21), 5789-5795 (1996)
   PUBMED   8918456
REFERENCE   8  (bases 1 to 1976)
  AUTHORS   Takei,K., Mundigl,O., Daniell,L. and De Camilli,P.
  TITLE     The synaptic vesicle cycle: a single vesicle budding step involving
            clathrin and dynamin
  JOURNAL   J Cell Biol 133 (6), 1237-1250 (1996)
   PUBMED   8682861
REFERENCE   9  (bases 1 to 1976)
  AUTHORS   Thurieau,C., Brosius,J., Burne,C., Jolles,P., Keen,J.H.,
            Mattaliano,R.J., Chow,E.P., Ramachandran,K.L. and Kirchhausen,T.
  TITLE     Molecular cloning and complete amino acid sequence of AP50, an
            assembly protein associated with clathrin-coated vesicles
  JOURNAL   DNA 7 (10), 663-669 (1988)
   PUBMED   3148444
REFERENCE   10 (bases 1 to 1976)
  AUTHORS   Keen,J.H. and Black,M.M.
  TITLE     The phosphorylation of coated membrane proteins in intact neurons
  JOURNAL   J Cell Biol 102 (4), 1325-1333 (1986)
   PUBMED   2870069
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000011.1.
            
            On Nov 24, 2020 this sequence version replaced NM_053837.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC087724.1, FQ213990.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                JAXUCZ010000011.1  93868453-93868480   c
            29-145              JAXUCZ010000011.1  93866081-93866197   c
            146-411             JAXUCZ010000011.1  93864223-93864488   c
            412-494             JAXUCZ010000011.1  93863315-93863397   c
            495-500             JAXUCZ010000011.1  93862925-93862930   c
            501-636             JAXUCZ010000011.1  93862632-93862767   c
            637-778             JAXUCZ010000011.1  93862358-93862499   c
            779-898             JAXUCZ010000011.1  93861845-93861964   c
            899-1034            JAXUCZ010000011.1  93861584-93861719   c
            1035-1132           JAXUCZ010000011.1  93861384-93861481   c
            1133-1244           JAXUCZ010000011.1  93860873-93860984   c
            1245-1976           JAXUCZ010000011.1  93859690-93860421   c
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q23"
     gene            1..1976
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /note="adaptor related protein complex 2 subunit mu 1"
                     /db_xref="GeneID:116563"
                     /db_xref="RGD:620135"
     exon            1..28
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            29..145
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..1379
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /note="mu2-adaptin; AP-2 mu chain; clathrin coat assembly
                     protein AP50; clathrin coat-associated protein AP50;
                     adaptor protein complex AP-2 subunit mu; plasma membrane
                     adaptor AP-2 50 kDa protein; adapter-related protein
                     complex 2 mu subunit; clathrin assembly protein complex 2
                     medium chain; coat assembly protein complex 50 kD;
                     adapter-related protein complex 2 subunit mu; clathrin
                     assembly protein complex 2 mu medium chain;
                     adaptor-related protein complex 2 subunit mu;
                     adaptor-related protein complex 2, mu 1 subunit"
                     /codon_start=1
                     /product="AP-2 complex subunit mu"
                     /protein_id="NP_446289.1"
                     /db_xref="GeneID:116563"
                     /db_xref="RGD:620135"
                     /translation="MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVR
                     SPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIK
                     NNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIG
                     WRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMND
                     KIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMR
                     YRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQ
                     VICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEV
                     PFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC"
     exon            146..411
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            412..494
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            495..500
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            501..636
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            637..778
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            779..898
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1034
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1132
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1244
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1976
                     /gene="Ap2m1"
                     /gene_synonym="Ap50; Clapm1; mu2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cctgccttgt gggggctgct gactccaggt ctgttctcag agcaatgggt cacagagact
       61 gagctgccgc catgatcgga ggcttattca tctataatca caagggggag gtgcttatct
      121 cccgagtcta cagagatgac atcgggagga atgcagtgga tgcctttcgc gtcaatgtca
      181 ttcacgcacg gcagcaggtg cgcagccctg tcacaaacat cgctcgcacc agcttcttcc
      241 atgttaagcg gtccaacatc tggctggcag cagtcaccaa gcagaatgtc aatgccgcca
      301 tggtcttcga attcctctac aagatgtgtg atgtaatggc tgcttacttt ggcaagatca
      361 gcgaggagaa catcaagaac aatttcgtgc tcatatacga gctgctggac gagattctgg
      421 actttggcta cccacagaac tcagagacag gtgcactgaa aaccttcatc acccaacagg
      481 gtatcaagag tcagcatcag acaaaagaag agcagtccca gatcaccagc caggtgaccg
      541 ggcaaattgg ctggaggcgg gaaggcatca agtatcgtcg gaatgaactc ttcctagatg
      601 ttctggagag tgtgaacctg cttatgtccc cacaggggca ggtgctgagt gcccatgtgt
      661 cgggccgggt ggtgatgaag agttacctga gcggcatgcc tgagtgcaag tttggaatga
      721 atgacaagat tgtcatagaa aagcagggca aaggcacagc cgatgaaaca agcaagagtg
      781 gtaagcagtc gatcgccatt gatgactgca cctttcacca gtgtgtgcga ctcagcaagt
      841 ttgactctga gcggagcatc agctttatcc ctcctgatgg agagtttgaa ctcatgagat
      901 accgtactac caaggacatc atcctgcctt tccgggtgat cccattggtg cgggaggtgg
      961 gacgcaccaa actggaggtc aaggtggtca tcaagtccaa cttcaagccc tcacttctgg
     1021 cccagaagat tgaggtgagg atcccaaccc cattgaacac aagcggggtg caagtgatct
     1081 gcatgaaggg gaaggccaag tacaaggcca gcgagaacgc cattgtatgg aagatcaagc
     1141 gcatggcagg catgaaggaa tcacagatca gcgcagagat tgagctcctg cccaccaatg
     1201 ataagaagaa atgggctcgg ccccccattt ccatgaactt tgaggtgcca tttgcgcctt
     1261 ctggcctcaa ggtgcgctac ttgaaggtat ttgagccgaa gctgaactac agcgaccacg
     1321 atgtcatcaa atgggtgcgc tacattggcc gaagtggcat ttacgaaacc cgctgctagc
     1381 tgccccgctg cggctggccc acatcctgcc caccctttcc acaggtccaa gcacccccag
     1441 tgaccgctca ccagtgtcgt tcctccctcc tgctctgcct ccttcccttc ccaatagccc
     1501 ctgagtatag gtctgaaccg gccacatcac agtgggactg gtcaggcagt ccctagcttc
     1561 ctgggagggt ggtttttgag gcttctgtct gtctgtcctg gcttagtgcc aggccctgag
     1621 ttttgtgacc aaagccaggt ggtcccctct tctctctacc ctgtggccac acctctgcag
     1681 tgggaaggtt gaataccctt acctcagagc tcccccaacg gccagtaacg gatccccagt
     1741 cattagtccc tactctgctt tgggatagta tgagcttcgt tttgtacacg tgtgactttg
     1801 tccagttata aacccaataa actgtagact ggaagtgctg gcccagtgtt aatggggctg
     1861 tggggagggt ctgatgggac tctgcagccc tggctgtcct ggaacctgtg tgtagactgg
     1921 cctcaaactt agagatctgc ctcgggagtg ctgggattaa agtgtgcacc accata
//
