LOCUS       NM_001395092            3409 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus adaptor related protein complex 2 subunit alpha 1
            (Ap2a1), transcript variant 1, mRNA.
ACCESSION   NM_001395092 XM_006229064
VERSION     NM_001395092.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Capella,P., Asensio,J., Troncoso,M., Sosa,M.A. and Seltzer,A.M.
  TITLE     Evidence on differential role for alpha 1 and alpha 2 subtypes of
            AP-2 adaptin in the central nervous system
  JOURNAL   Neurosci Lett 808, 137282 (2023)
   PUBMED   37127089
  REMARK    GeneRIF: Evidence on differential role for alpha 1 and alpha 2
            subtypes of AP-2 adaptin in the central nervous system.
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Taoufiq,Z., Ninov,M., Villar-Briones,A., Wang,H.Y., Sasaki,T.,
            Roy,M.C., Beauchain,F., Mori,Y., Yoshida,T., Takamori,S., Jahn,R.
            and Takahashi,T.
  TITLE     Hidden proteome of synaptic vesicles in the mammalian brain
  JOURNAL   Proc Natl Acad Sci U S A 117 (52), 33586-33596 (2020)
   PUBMED   33376223
REFERENCE   3  (bases 1 to 3409)
  AUTHORS   Moulakakis,C., Steinhauser,C., Biedziak,D., Freundt,K., Reiling,N.
            and Stamme,C.
  TITLE     Surfactant Protein A Enhances Constitutive Immune Functions of
            Clathrin Heavy Chain and Clathrin Adaptor Protein 2
  JOURNAL   Am J Respir Cell Mol Biol 55 (1), 92-104 (2016)
   PUBMED   26771574
  REMARK    GeneRIF: SP-A transiently enhances the basal protein expression of
            CHC and alpha-adaptin.
REFERENCE   4  (bases 1 to 3409)
  AUTHORS   Zhao,Z., Sagare,A.P., Ma,Q., Halliday,M.R., Kong,P., Kisler,K.,
            Winkler,E.A., Ramanathan,A., Kanekiyo,T., Bu,G., Owens,N.C.,
            Rege,S.V., Si,G., Ahuja,A., Zhu,D., Miller,C.A., Schneider,J.A.,
            Maeda,M., Maeda,T., Sugawara,T., Ichida,J.K. and Zlokovic,B.V.
  TITLE     Central role for PICALM in amyloid-beta blood-brain barrier
            transcytosis and clearance
  JOURNAL   Nat Neurosci 18 (7), 978-987 (2015)
   PUBMED   26005850
  REMARK    Erratum:[Nat Neurosci. 2024 Jan;27(1):208. PMID: 37985802]
REFERENCE   5  (bases 1 to 3409)
  AUTHORS   Onishi,K., Shafer,B., Lo,C., Tissir,F., Goffinet,A.M. and Zou,Y.
  TITLE     Antagonistic functions of Dishevelleds regulate Frizzled3
            endocytosis via filopodia tips in Wnt-mediated growth cone guidance
  JOURNAL   J Neurosci 33 (49), 19071-19085 (2013)
   PUBMED   24305805
REFERENCE   6  (bases 1 to 3409)
  AUTHORS   Di Paolo,G., Sankaranarayanan,S., Wenk,M.R., Daniell,L.,
            Perucco,E., Caldarone,B.J., Flavell,R., Picciotto,M.R., Ryan,T.A.,
            Cremona,O. and De Camilli,P.
  TITLE     Decreased synaptic vesicle recycling efficiency and cognitive
            deficits in amphiphysin 1 knockout mice
  JOURNAL   Neuron 33 (5), 789-804 (2002)
   PUBMED   11879655
REFERENCE   7  (bases 1 to 3409)
  AUTHORS   Kamiguchi,H. and Yoshihara,F.
  TITLE     The role of endocytic l1 trafficking in polarized adhesion and
            migration of nerve growth cones
  JOURNAL   J Neurosci 21 (23), 9194-9203 (2001)
   PUBMED   11717353
REFERENCE   8  (bases 1 to 3409)
  AUTHORS   Rotem-Yehudar,R., Galperin,E. and Horowitz,M.
  TITLE     Association of insulin-like growth factor 1 receptor with EHD1 and
            SNAP29
  JOURNAL   J Biol Chem 276 (35), 33054-33060 (2001)
   PUBMED   11423532
REFERENCE   9  (bases 1 to 3409)
  AUTHORS   Chen,H., Slepnev,V.I., Di Fiore,P.P. and De Camilli,P.
  TITLE     The interaction of epsin and Eps15 with the clathrin adaptor AP-2
            is inhibited by mitotic phosphorylation and enhanced by
            stimulation-dependent dephosphorylation in nerve terminals
  JOURNAL   J Biol Chem 274 (6), 3257-3260 (1999)
   PUBMED   9920862
REFERENCE   10 (bases 1 to 3409)
  AUTHORS   Slepnev,V.I., Ochoa,G.C., Butler,M.H., Grabs,D. and De Camilli,P.
  TITLE     Role of phosphorylation in regulation of the assembly of endocytic
            coat complexes
  JOURNAL   Science 281 (5378), 821-824 (1998)
   PUBMED   9694653
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Jun 3, 2021 this sequence version replaced XM_006229064.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN12840115, SAMN16676788
                              [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-180               JAXUCZ010000001.1  104550476-104550655 c
            181-249             JAXUCZ010000001.1  104538221-104538289 c
            250-392             JAXUCZ010000001.1  104537984-104538126 c
            393-586             JAXUCZ010000001.1  104537632-104537825 c
            587-716             JAXUCZ010000001.1  104530238-104530367 c
            717-818             JAXUCZ010000001.1  104529758-104529859 c
            819-927             JAXUCZ010000001.1  104528057-104528165 c
            928-1078            JAXUCZ010000001.1  104527284-104527434 c
            1079-1247           JAXUCZ010000001.1  104526790-104526958 c
            1248-1385           JAXUCZ010000001.1  104526557-104526694 c
            1386-1568           JAXUCZ010000001.1  104526075-104526257 c
            1569-1666           JAXUCZ010000001.1  104525036-104525133 c
            1667-1898           JAXUCZ010000001.1  104524671-104524902 c
            1899-2066           JAXUCZ010000001.1  104524423-104524590 c
            2067-2227           JAXUCZ010000001.1  104524175-104524335 c
            2228-2293           JAXUCZ010000001.1  104523892-104523957 c
            2294-2350           JAXUCZ010000001.1  104523575-104523631 c
            2351-2433           JAXUCZ010000001.1  104523393-104523475 c
            2434-2523           JAXUCZ010000001.1  104523171-104523260 c
            2524-2647           JAXUCZ010000001.1  104522007-104522130 c
            2648-2764           JAXUCZ010000001.1  104521804-104521920 c
            2765-2834           JAXUCZ010000001.1  104521638-104521707 c
            2835-2969           JAXUCZ010000001.1  104521382-104521516 c
            2970-3409           JAXUCZ010000001.1  104520792-104521231 c
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..3409
                     /gene="Ap2a1"
                     /note="adaptor related protein complex 2 subunit alpha 1"
                     /db_xref="GeneID:308578"
                     /db_xref="RGD:1306254"
     exon            1..180
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     CDS             114..3047
                     /gene="Ap2a1"
                     /note="isoform 1 is encoded by transcript variant 1; AP-2
                     complex subunit alpha-1; adaptor protein complex AP-2,
                     alpha 1 subunit; alpha-adaptin A; adaptor-related protein
                     complex 2, alpha 1 subunit"
                     /codon_start=1
                     /product="AP-2 complex subunit alpha-1 isoform 1"
                     /protein_id="NP_001382021.1"
                     /db_xref="GeneID:308578"
                     /db_xref="RGD:1306254"
                     /translation="MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKF
                     KGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLV
                     NSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDS
                     MDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKN
                     PDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAV
                     KGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQL
                     GQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADL
                     LYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLI
                     RIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFG
                     NLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAG
                     SQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGA
                     ASALDDSRRDTSSNDINGGVEPTPSNVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLV
                     DVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMTLLADPAPAADPGPEDIGPPIPEA
                     DELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQSFLPTVVHP
                     GDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLK
                     LPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSA
                     LLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCEL
                     LAQQF"
     exon            181..249
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            250..392
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            393..586
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            587..716
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            717..818
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            819..927
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1078
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1247
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1385
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1568
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1666
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1898
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2066
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2227
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2228..2293
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2350
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2351..2433
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2434..2523
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2524..2647
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2648..2764
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..2834
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2835..2969
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2970..3409
                     /gene="Ap2a1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcccgccct cccccggccg gctcctcggc gccgtctggg gtcctttccg cccggtgctg
       61 ccagccagcc ccgcgcccgt tcggccaggc ctggagccga gcccttcgcc atcatgccgg
      121 ctgtatccaa aggcgatggc atgcgtgggc ttgccgtgtt catctccgac atccggaact
      181 gcaagagcaa agaggctgag atcaagagga tcaacaagga actggccaac atccgctcca
      241 agttcaaagg ggacaaggcc ttggatggct acagtaaaaa gaagtatgtg tgtaagctgc
      301 tcttcatctt cctgcttggc catgacattg actttggaca catggaggct gtgaacctgc
      361 taagctccaa caagtacaca gagaagcaga tagggtacct gttcatctca gtcctggtga
      421 actcgaactc agagctgatc cggctcatta acaacgctat caagaacgac ctggccagtc
      481 gcaaccccac cttcatgtgc ctggctttgc actgtattgc caacgtgggc agccgtgaga
      541 tgggcgaggc ctttgctgcc gacattcccc gaatcctggt ggccggagac agcatggata
      601 gtgtgaagca gagtgcagcc ctatgcctac tgcgtctcta caaggcctcc cccgacttgg
      661 tgcccatggg cgagtggacg gcccgggtgg tgcatttgct caatgatcag cacatgggag
      721 tggtcacagc cgctgtcagt ctcatcacct gcctgtgcaa gaagaatcca gatgacttca
      781 agacctgtgt ctccctggct gtgtctcgcc taagccggat tgtctcctca gcctccactg
      841 acctccagga ctacacttac tacttcgttc ctgcaccctg gctctctgtg aagttactgc
      901 ggttgctaca gtgttaccca ccgccagagg atgcggctgt gaaagggcgg ttagtggagt
      961 gtcttgagac tgtgctcaac aaggcccagg agcctcccaa gtccaagaag gtgcagcact
     1021 ccaatgccaa gaacgccatc ctgtttgaga ctattagcct catcatccac tatgacagtg
     1081 agcccaacct cctggtccgt gcctgcaacc agctgggcca gttcctgcag caccgagaga
     1141 cgaacctgcg ctacctggct ctggagagca tgtgcaccct ggccagctcc gagttctccc
     1201 acgaggctgt caagacccac attgatactg ttattaatgc cctcaagact gagcgggacg
     1261 tgagtgtgag gcagcgggca gctgatctgc tgtatgccat gtgtgaccgg agcaatgcca
     1321 agcagattgt atcagagatg ctgcggtacc tagagacggc tgactatgcc atccgagagg
     1381 agattgtgct gaaggtggcc atcttggctg agaagtatgc agtggactac agctggtatg
     1441 tggacaccat cctcaacctc atccgcattg caggcgacta tgtgagcgag gaggtgtggt
     1501 accgagtgct gcagatcgtc accaaccgtg atgatgtcca gggttatgct gccaagacag
     1561 tgtttgaggc cctccaggcc ccagcctgtc atgagaacat ggtgaaggtc ggtggctaca
     1621 tccttgggga gtttgggaac ttgattgctg gggacccacg ctccagccca cctgtgcagt
     1681 tctcactgct gcactccaag ttccacctgt gcagtgtggc cactcgtgcc ttgctgctgt
     1741 ccacctacat caagttcatc aacctcttcc ctgagaccaa ggccaccatc caaggtgttc
     1801 ttcgtgccgg ctcccagctg cgcaatgctg acgtggagct acagcagcgg gcagtggagt
     1861 acctcaccct cagctctgta gctagcaccg atgttttggc tactgtgcta gaagaaatgc
     1921 ccccatttcc tgagcgggag tcatccattt tggctaagct gaagcgtaag aagggccctg
     1981 gggcagccag tgccctggat gacagccgca gggacaccag cagcaatgac atcaatgggg
     2041 gtgtggagcc cacccccagc aatgtgtcaa ccccctcacc ctccgcggac ctcttggggc
     2101 tgcgggcagc ccctccccct gctgcacccc cagctcctgt aggcgggaac ctcctggtgg
     2161 atgttttctc tgacggcccc actgcacagc ccagcctggg tcccacccct gaggaggcct
     2221 tcctcagcga gctggagccc cctgcccctg agagccccat gactttgttg gctgacccag
     2281 ctccagctgc tgacccaggt cctgaggaca taggtcctcc cattccagaa gcagatgaac
     2341 tgctgaataa gttcgtgtgt aagaatagtg gggtcttgtt tgagaaccag ctgctgcaga
     2401 tcggagtcaa gtctgagttc cggcagaacc tgggccgcat gtatctcttc tatggcaaca
     2461 agacttctgt gcagttccag agcttcttgc ccaccgtggt ccaccctggg gacctccaga
     2521 ctcagctggc ggtgcagacc aagcgtgtag ctgcacaagt ggacggtggc gcacaggtgc
     2581 agcaagtact caacattgag tgtctgcgag acttcttgac gccaccactg ctgtcggtgc
     2641 gcttccggta cggtggcacc gcccagtccc tcactctgaa gctcccagtg accatcaaca
     2701 aattcttcca gcccacagag atggccgccc aggacttttt ccagcgctgg aagcagctga
     2761 gcctccccct gcaggaggca cagaaaatct tcaaagccaa ccaccccatg gatgctgaag
     2821 tcacaaaggc caagcttctg gggtttggct ctgctcttct ggacaatgtg gatcccaacc
     2881 ctgagaactt tgtgggtgct ggaatcatcc agacgaaagc cctgcaggtg gggtgtctgc
     2941 ttcgactgga gcccaatgcc caggcccaaa tgtaccgcct aaccctgcgc accagcaaag
     3001 agcctgtgtc ccgtcactta tgtgagctgc tggcccagca gttctgagcc ctggacttgc
     3061 cctgggatat ggccggtgcc aggcagccct gagatggagg cagctgtggt ggagggggac
     3121 agcgagggga cctccagtgg tgacagcaaa gaccccaggg atgggggcat gcctggcgcg
     3181 tcctctggcc tttgtatttt atttttgttc atctgctgct gtttacattc tggggggtca
     3241 agggaagcac cctcccttcc ttgtcccccg aagcacagag gggagggggg ccggggaagt
     3301 aggtacctcc tcccctcccc ctccctcctt ttcaccctcc tccctccctc cctctccccg
     3361 tccaggggcc gtgtattatt gtgagcgaat aaacagagag acgctaata
//
