LOCUS       NM_031012               3320 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Rattus norvegicus alanyl aminopeptidase, membrane (Anpep), mRNA.
ACCESSION   NM_031012
VERSION     NM_031012.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Cheng,R., Xu,X., Yang,S., Mi,Z., Zhao,Y., Wang,C., Shi,X., Gao,J.,
            Yu,F. and Ren,X.
  TITLE     The effect of APN, hs-CRP and APN/hs-CRP in periodontitis with DAA
  JOURNAL   BMC Oral Health 23 (1), 85 (2023)
   PUBMED   36765308
  REMARK    GeneRIF: The effect of APN, hs-CRP and APN/hs-CRP in periodontitis
            with DAA.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Minocha,E., Chaturvedi,C.P. and Nityanand,S.
  TITLE     Renogenic characterization and in vitro differentiation of rat
            amniotic fluid stem cells into renal proximal tubular- and
            juxtaglomerular-like cells
  JOURNAL   In Vitro Cell Dev Biol Anim 55 (2), 138-147 (2019)
   PUBMED   30645697
REFERENCE   3  (bases 1 to 3320)
  AUTHORS   Dominguez-Vias,G., Aretxaga-Maza,G., Prieto,I., Segarra,A.B.,
            Luna,J.D., Gasparo,M. and Ramirez-Sanchez,M.
  TITLE     Light-dark influence on enkephalinase activity in hypothalamus and
            pituitary
  JOURNAL   Neuro Endocrinol Lett 39 (4), 277-280 (2018)
   PUBMED   30531704
  REMARK    GeneRIF: In standard light/dark conditions, the results
            demonstrated an opposite daily rhythm in hypothalamus and
            pituitary. While the highest levels of AlaAP or enkephalinase
            activities were measured in hypothalamus during the dark period,
            they were the highest in the pituitary during the light one.
REFERENCE   4  (bases 1 to 3320)
  AUTHORS   Byzia,A., Szeffler,A., Kalinowski,L. and Drag,M.
  TITLE     Activity profiling of aminopeptidases in cell lysates using a
            fluorogenic substrate library
  JOURNAL   Biochimie 122, 31-37 (2016)
   PUBMED   26449746
  REMARK    GeneRIF: Data indicate that fluorogenic substrates can be
            successfully used to identify aminopeptidase N and to measure their
            activity in cell lysates.
REFERENCE   5  (bases 1 to 3320)
  AUTHORS   Thaysen-Andersen,M., Venkatakrishnan,V., Loke,I., Laurini,C.,
            Diestel,S., Parker,B.L. and Packer,N.H.
  TITLE     Human neutrophils secrete bioactive paucimannosidic proteins from
            azurophilic granules into pathogen-infected sputum
  JOURNAL   J Biol Chem 290 (14), 8789-8802 (2015)
   PUBMED   25645918
REFERENCE   6  (bases 1 to 3320)
  AUTHORS   Yeager,C.L., Ashmun,R.A., Williams,R.K., Cardellichio,C.B.,
            Shapiro,L.H., Look,A.T. and Holmes,K.V.
  TITLE     Human aminopeptidase N is a receptor for human coronavirus 229E
  JOURNAL   Nature 357 (6377), 420-422 (1992)
   PUBMED   1350662
REFERENCE   7  (bases 1 to 3320)
  AUTHORS   Funkhouser,J.D., Tangada,S.D., Jones,M., O,S.J. and Peterson,R.D.
  TITLE     p146 type II alveolar epithelial cell antigen is identical to
            aminopeptidase N
  JOURNAL   Am J Physiol 260 (4 Pt 1), L274-L279 (1991)
   PUBMED   1673322
REFERENCE   8  (bases 1 to 3320)
  AUTHORS   Malfroy,B., Kado-Fong,H., Gros,C., Giros,B., Schwartz,J.C. and
            Hellmiss,R.
  TITLE     Molecular cloning and amino acid sequence of rat kidney
            aminopeptidase M: a member of a super family of
            zinc-metallohydrolases
  JOURNAL   Biochem Biophys Res Commun 161 (1), 236-241 (1989)
   PUBMED   2567164
REFERENCE   9  (bases 1 to 3320)
  AUTHORS   Watt,V.M. and Yip,C.C.
  TITLE     Amino acid sequence deduced from a rat kidney cDNA suggests it
            encodes the Zn-peptidase aminopeptidase N
  JOURNAL   J Biol Chem 264 (10), 5480-5487 (1989)
   PUBMED   2564389
REFERENCE   10 (bases 1 to 3320)
  AUTHORS   Look,A.T., Ashmun,R.A., Shapiro,L.H. and Peiper,S.C.
  TITLE     Human myeloid plasma membrane glycoprotein CD13 (gp150) is
            identical to aminopeptidase N
  JOURNAL   J Clin Invest 83 (4), 1299-1307 (1989)
   PUBMED   2564851
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from M25073.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M25073.1, M26710.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132280,
                                           SAMD00132282 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q31"
     gene            1..3320
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /note="alanyl aminopeptidase, membrane"
                     /db_xref="GeneID:81641"
                     /db_xref="RGD:2991"
     exon            1..633
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..2917
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /EC_number="3.4.11.2"
                     /note="leucine arylaminopeptidase 1; aminopeptidase N;
                     cluster of differentiation antigen 13 (CD13);
                     aminopeptidase M; kidney Zn peptidase; microsomal
                     aminopeptidase; membrane alanine aminopeptidase; kidney
                     aminopeptidase M; alanyl (membrane) aminopeptidase"
                     /codon_start=1
                     /product="aminopeptidase N precursor"
                     /protein_id="NP_112274.1"
                     /db_xref="GeneID:81641"
                     /db_xref="RGD:2991"
                     /translation="MAKGFYISKTLGILGILLGVAAVCTIIALSVVYAQEKNRNAENS
                     AIAPTLPGSTSATTSTTNPAIDESKPWNQYRLPKTLIPDSYQVTLRPYLTPNEQGLYI
                     FKGSSTVRFTCNETTNVIIIHSKKLNYTNKGNHRVALRALGDTPAPNIDTTELVERTE
                     YLVVHLQGSLVKGHQYEMDSEFQGELADDLAGFYRSEYMEGGNKKVVATTQMQAADAR
                     KSFPCFDEPAMKASFNITLIHPNNLTALSNMLPKDSRTLQEDPSWNVTEFHPTPKMST
                     YLLAYIVSEFKYVEAVSPNRVQIRIWARPSAIDEGHGDYALQVTGPILNFFAQHYNTA
                     YPLEKSDQIALPDFNAGAMENWGLVTYRESALVFDPQSSSISNKERVVTVIAHELAHQ
                     WFGNLVTVDWWNDLWLNEGFASYVEFLGADYAEPTWNLKDLIVLNDVYRVMAVDALAS
                     SHPLSSPANEVNTPAQISELFDSITYSKGASVLRMLSSFLTEDLFKKGLSSYLHTFQY
                     SNTIYLDLWEHLQQAVDSQTAIKLPASVSTIMDRWILQMGFPVITVNTSTGEIYQEHF
                     LLDPTSKPTRPSDFNYLWIVPIPYLKNGKEDHYWLETEKNQSAEFQTSSNEWLLLNIN
                     VTGYYQVNYDENNWRKIQNQLQTDLSVIPVINRAQIIHDSFNLASAGKLSITLPLSNT
                     LFLASETEYMPWEAALSSLNYFKLMFDRSEVYGPMKRYLKKQVTPLFAYFKIKTNNWL
                     DRPPTLMEQYNEINAISTACSSGLEECRDLVVGLYSQWMNNSDNNPIHPNLRSTVYCN
                     AIAFGGEEEWNFAWEQFRKATLVNEADKLRSALACSNEVWILNRYLSYTLNPDYIRKQ
                     DATSTIVSIANNVVGQTLVWDFVRSNWKKLFEDYGGGSFSFANLIQGVTRRFSSEFEL
                     QQLEQFKEDNSATGFGSGTRALEQALEKTKANIKWVKENKDVVLKWFTENS"
     exon            634..776
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            777..913
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1040
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1195
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1309
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1453
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1519
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1585
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1761
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1762..1835
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1969
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            1970..2025
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2173
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..2265
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2376
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2544
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            2545..2685
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2767
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
     exon            2768..3320
                     /gene="Anpep"
                     /gene_synonym="AP-M; AP-N; Apm; Apn; KZP; Lap1; rAPN"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctccctgccc accagcatca tggccaaggg tttctacatt tccaagaccc tgggcatctt
       61 gggcatcctg ttaggtgtgg cagccgtatg caccatcata gctctgtcgg tggtctacgc
      121 tcaggagaag aacaggaatg cggagaactc tgccatagcc cccacgctcc caggcagcac
      181 ctcagccacc acctcaacta ccaaccctgc tatagatgaa agcaaacctt ggaaccagta
      241 tcgcttgcct aagactctta tacccgactc ctaccaggtg accttgaggc cttacctcac
      301 ccccaacgag cagggcctgt acatcttcaa aggttccagt actgtccgct ttacctgcaa
      361 cgagaccaca aatgtcatca ttatccacag caagaagctc aactacacca acaaagggaa
      421 ccacagggtg gcgttgcgag ccctgggtga cactccggca cctaacatcg acacaacgga
      481 actggtagag cgcacggagt acctggtggt gcacctgcag ggctccctgg taaagggcca
      541 tcagtacgag atggacagtg agttccaggg ggagctggct gatgatctgg ctggcttcta
      601 ccgcagcgag tacatggaag gtggcaacaa gaaagtggtg gccaccacac agatgcaggc
      661 tgccgatgct cgaaaatcct ttccttgctt cgatgagcca gccatgaagg cctcattcaa
      721 catcacgctc atccacccca acaacctcac cgctctgtct aacatgcttc ccaaagattc
      781 caggaccctt caggaagatc cttcctggaa cgtcactgag ttccacccta ccccgaagat
      841 gtccacatac ctgctggcct atatcgtgag cgagttcaaa tatgtagaag ccgtctcacc
      901 caatagagtc cagattcgaa tctgggctcg gcccagtgcc attgatgagg gccatggtga
      961 ttacgctctg caagtcacag gccctatcct aaacttcttt gcccaacatt ataatacagc
     1021 ctaccctcta gaaaaatctg accagattgc cctgcctgac ttcaacgctg gagccatgga
     1081 gaactggggg ctcgtgacct accgagagag tgccctggtc tttgaccctc agtcctcctc
     1141 cattagcaac aaggagcggg tggtcactgt gattgcccat gagctggccc atcagtggtt
     1201 tggcaacctg gtgaccgtgg attggtggaa tgacctgtgg ctgaacgagg gctttgcctc
     1261 ctatgtggaa tttctgggtg ctgactatgc agagcccacc tggaatctga aagacctcat
     1321 agtactgaat gatgtgtacc gtgtgatggc cgtggacgcg ctcgcttcct cccacccgtt
     1381 gtccagccct gccaacgagg tcaacacgcc agcccagatc agtgagctgt ttgacagcat
     1441 cacctacagc aagggagcct cagtccttag gatgttgtcc agcttcctga cagaggacct
     1501 gttcaagaag ggcctttcgt cttacctcca caccttccaa tactcgaaca ccatttatct
     1561 ggacctgtgg gaacaccttc agcaggcagt ggacagtcag acagctatca aactcccagc
     1621 ctcagtgagc accatcatgg accgatggat tctacagatg ggttttcctg ttatcactgt
     1681 gaacaccagt acgggagaaa tctaccagga acacttcctc ctggacccca cttccaaacc
     1741 tacccgcccc tcagatttta attacctctg gattgtgccc attccgtatc tcaaaaatgg
     1801 aaaggaggat cactactggc tggagaccga gaaaaaccag agtgcagaat tccaaacatc
     1861 ctccaatgaa tggctcttac tgaacattaa tgtaaccggc tactaccagg ttaactatga
     1921 tgaaaacaac tggaggaaga ttcagaatca gctgcaaaca gacctttctg ttatccctgt
     1981 catcaaccgt gcgcagatta tccacgactc cttcaaccta gccagtgctg gaaagctatc
     2041 catcactctg ccgctgagca acaccctctt cctggccagt gaaacagaat acatgccctg
     2101 ggaggctgcc ctgagcagcc tgaactactt caagctcatg tttgaccgct cggaggtcta
     2161 cggccccatg aagcgctatc tgaagaagca agtcacaccc ctcttcgcct acttcaaaat
     2221 caaaaccaac aactggctcg accgtcctcc aacactgatg gagcagtaca acgaaattaa
     2281 tgccatcagc accgcctgtt ctagtggtct cgaagagtgt agggatctcg ttgttgggct
     2341 ttatagtcag tggatgaaca actctgataa taacccgatc caccccaacc ttcggtctac
     2401 tgtctactgc aatgccattg ctttcggtgg tgaggaagag tggaactttg cttgggaaca
     2461 gttccggaaa gcaactctgg tgaacgaggc agataaactc cgctcagcct tggcctgtag
     2521 caacgaggtg tggattttga acaggtacct gagttacact ctgaaccccg actacatccg
     2581 gaagcaggat gctacctcca ccatcgtcag catcgccaac aacgtggttg gacaaactct
     2641 agtttgggac tttgtccgaa gcaactggaa gaaactgttt gaagattacg gcggaggatc
     2701 tttctccttt gccaatctca tccagggagt gacccggcgc ttctcctctg agttcgagct
     2761 gcagcagctg gagcagttta aagaggacaa ctcggccaca ggctttggct ccggcactcg
     2821 ggctctggag caagccctgg agaagactaa agccaacatc aagtgggtga aggagaacaa
     2881 agacgtggta ctcaagtggt tcacagaaaa cagctagtta gtcccgggcc gagaaccatc
     2941 tgtcccggcg tgacgcctat gactatctca gcagcctgtg cagggtctct gtcttcagag
     3001 cgccagacac cggcatctga ccctcaaggt caaagtctcc agcctatgga gctagcctag
     3061 ctcctaattg tcagactgcc agacacctcc cggtccttgc ccctcgtgcc aactctgccc
     3121 caggcccagg cctctggggc tgatctcagg gaagcccagc tctgagtcta gatttactgg
     3181 acaaagggta gcctagaaag agtctccctg aatgccttac tactttcccc ccacaagacc
     3241 cagaaccaaa gaatcaacag ggcacaaaga tctatatata tttttaagag aaaatgtaaa
     3301 taaagaattt ctaaaatgag
//
