LOCUS       XM_039078688            4463 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus anoctamin 4 (Ano4), transcript variant
            X2, mRNA.
ACCESSION   XM_039078688
VERSION     XM_039078688.1
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051342.1) annotated using gene prediction method: Gnomon,
            supported by mRNA and EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Feb 22, 2024 this sequence was replaced by
            XM_039078688.2.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..4463
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /note="anoctamin 4; Derived by automated computational
                     analysis using gene prediction method: Gnomon. Supporting
                     evidence includes similarity to: 1 mRNA, 4 ESTs, 3
                     Proteins, and 100% coverage of the annotated genomic
                     feature by RNAseq alignments, including 6 samples with
                     support for all annotated introns"
                     /db_xref="GeneID:299714"
                     /db_xref="RGD:1310252"
     CDS             229..3648
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /codon_start=1
                     /product="anoctamin-4 isoform X2"
                     /protein_id="XP_038934616.1"
                     /db_xref="GeneID:299714"
                     /db_xref="RGD:1310252"
                     /translation="MHSPGRGLAVQGRGAQAAALADALASLRRTRARMASLTAYDKDV
                     SSGSYNLSSATTGSYYSCVSQITIGYDVAGPVAIGLTHWKNPYVQGRPTLPSDLPLFL
                     SKDTSIPVYHQSFTRKADLATPLSCPASLSITPVPSYSSSSQETLSQDTTGLSLSIHG
                     AEKRLQIHQRRSVASRPGSRRRLPVVRHSSLPPGRRSIKMEASSSGITNGKNKVFHAE
                     GGVDLQNHQLDMQTLSDGPKSDVDFSEILNAIQEMAKDVNIFFDELEAVNSPCKDDDS
                     LLHPGNLTSTSEDTSRLEAGGEKVRERNKSNGLYFRDGKCRIDYILVYRKSNPQTEKR
                     EVFERNIRAEGLQMEKESSLINSDIIFVKLHAPWEVLGRYAEQMNVRMPFRIDKQISR
                     FRRWLPKKPMRLDKETLPDLEENDCYTAPFSQQRIHHFIIHNKDTFFNNATRSRIVHH
                     ILQRIKYEEGKNKIGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTHGAVNHRHLLYECW
                     ASWSVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHC
                     QVSKEVCQATDIIMCPVCDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATV
                     FLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERMNPISGKPEPYQAFTDK
                     CSRLIVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVC
                     INFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFF
                     LGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQ
                     NWWTRRKVRQEHGTERKINFPQWEKDYNLQPMNAYGLFDEYLEMILQFGFTTIFVAAF
                     PLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFV
                     IAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGS
                     EFSGTPLKYCRYRDYRDPPHSLAPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISY
                     LIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKERKKNGKAHHNEWP"
ORIGIN      
        1 tcaggtcagg gaagcagcat ttggctcgca ccctgctggg tcccaagggc ccccgccctc
       61 ctctctgctc cgtcgcgctt gcgtcccggc gggcggcccc ccggagcgtg cccgctcccc
      121 acgccctcgc cgccgaaccc caagccgagg ctcctgcacc tctccggatc gcggcgggct
      181 gactgggcat gctccggagc ctggccccgc gccgtgggag ccgagcgcat gcactctccg
      241 ggccgggggc tcgccgtcca ggggcgcggc gctcaggcgg ccgctctcgc cgatgcgctg
      301 gccagcttgc gccgcacgcg agccaggatg gcttccctca ccgcctacga caaagacgtg
      361 agcagcggga gttataacct gtcctctgca acaaccggta gttactacag ctgtgtcagc
      421 cagatcacca taggttatga tgttgctggg cctgttgcga ttggactgac ccactggaag
      481 aatccgtatg tacaaggcag acccaccctc ccatcagatc tgcccctttt cctctccaaa
      541 gatacaagca tccctgtgta ccaccagtcc ttcactcgca aagctgacct ggccacacct
      601 ttgtcttgtc cagcctcgct atccatcact ccagtgcctt cttacagcag cagcagccag
      661 gaaactctga gccaagacac aacaggttta agtttatcta ttcatggggc tgaaaagcgc
      721 ttgcaaatcc atcaacggcg aagtgtggca agccgcccag ggtcacgacg tcgcctgcct
      781 gtggtcaggc attcctcact cccaccaggc agacggtcaa taaaaatgga ggccagctct
      841 tctggaatca ctaatggaaa aaacaaagtt ttccatgcag aaggtggagt ggatttgcaa
      901 aaccatcagc tggatatgca aacactgtcc gacggcccaa agagcgatgt ggacttttca
      961 gagattctta atgcaataca agaaatggcc aaggatgtca acattttttt tgatgaacta
     1021 gaggctgtca acagcccgtg caaagacgat gattctctcc ttcaccctgg aaatctgacc
     1081 agtacgtcgg aggacaccag cagactggaa gcagggggag agaaagtgcg agaacgaaac
     1141 aaatcaaatg gactttactt tagagacgga aaatgtcgaa ttgactacat ccttgtgtac
     1201 agaaaatcca accctcagac tgaaaagaga gaagtgtttg aaagaaatat tagagcagaa
     1261 ggattacaga tggagaaaga gtcctctcta ataaatagtg acattatctt tgtgaagttg
     1321 catgccccat gggaagtcct tggaagatat gcagagcaaa tgaatgtacg gatgcctttc
     1381 aggatcgata aacaaataag caggttccgg agatggttac ctaagaagcc aatgaggctg
     1441 gacaaggaga cactgccaga cctggaagag aatgactgct acactgcccc tttcagccag
     1501 caaaggattc atcacttcat catacacaac aaagacactt tcttcaacaa cgccaccaga
     1561 agcagaatcg tgcatcatat tttacaaagg ataaagtacg aggaaggaaa aaacaaaatc
     1621 ggtctgaacc gcttgcttac caatggctcc tatgaagctg cttttcccct gcatgaggga
     1681 agttatagaa gtaaaaactc cattaaaacc catggagctg taaaccaccg gcatctactg
     1741 tatgagtgct gggcctcctg gagtgtgtgg tacaagtacc agcctttgga tcttgtaagg
     1801 cggtattttg gagagaagat tggactgtat tttgcctggc tgggatggta cacaggcatg
     1861 ctcttcccgg cagccttcat tggcttgttt gtcttcttgt atggagtcac cactctggat
     1921 cactgccaag tcagtaaaga agtctgccaa gcaacagata tcatcatgtg tcctgtgtgt
     1981 gacaagtact gtccattcat gaggctatca gacagctgcg tctatgccaa ggtgacccac
     2041 ctttttgaca acggggctac tgtgttcttt gctgtgttca tggcagtctg ggccactgtt
     2101 ttcctggagt tctggaagag acgccgagcg gtgatcgctt atgactggga tctgattgac
     2161 tgggaagaag aggaggaaga aatacggccc cagtttgaag ccaagtattc caagaaagag
     2221 cggatgaatc ccatttcggg aaagccagaa ccttatcagg catttactga taaatgcagc
     2281 agacttatcg tttctgcatc gggaatattc ttcatgatct gtgtggtgat cgcggccgtg
     2341 ttcgggatcg tcatttaccg ggtggtgacc gtgagcactt tcgccgcctt taagtgggcg
     2401 ttaatcagga acaactctca ggtcgcgacc acagggactg cagtgtgcat caacttctgt
     2461 atcattatgc tgctgaacgt gctctatgaa aaagtcgcac tccttctgac aaatctggaa
     2521 cagcctcgca cagaatccga gtgggagaac agctttacct tgaaaatgtt cctttttcag
     2581 ttcgtcaatc tgaacagctc tactttttac atcgcattct tccttggaag atttacagga
     2641 cacccaggtg cctacttgag attgataaac aggtggaggc tagaagagtg ccaccctagt
     2701 ggatgcctta ttgatctctg catgcaaatg ggtattataa tggtgctaaa gcagacctgg
     2761 aataatttca tggaacttgg ctacccgtta attcagaatt ggtggactag aagaaaagtc
     2821 cgacaagaac atgggactga aaggaaaata aatttcccac agtgggaaaa ggactacaat
     2881 cttcagccga tgaatgccta tggactcttc gatgaatacc tggaaatgat ccttcagttc
     2941 ggattcacaa ctatctttgt ggcagctttt cctctagcac cgcttctggc cttactgaat
     3001 aacatcattg aaatccgact tgatgcttac aagtttgtga ctcagtggag aaggcctctg
     3061 gcttcgaggg ccaaggacat aggaatttgg tatggaattc tcgaaggcat cgggattctc
     3121 tctgttatta cgaacgcgtt tgtcatagca ataacatccg acttcatccc tcgcttagta
     3181 tatgcttata aatatggacc gtgtgcaggc caaggagaag ctgggcagaa gtgcatggtt
     3241 ggctatgtaa atgccagctt gtccgtgttc cgaatttctg actttgaaaa ccggtctgag
     3301 ccagagtctg atggcagtga gttctcagga actcctctga agtactgcag atatcgggac
     3361 taccgggacc cgcctcattc tttggctccc tatggctaca cactgcagtt ctggcatgtc
     3421 ctcgcagctc ggctggcttt catcattgtg tttgagcacc tcgtgttttg tataaagcac
     3481 ctcatttcct atctgatacc agacctcccg aaagatctaa gggaccggat gaggagagag
     3541 aagtacttga tccaggagat gatgtatgag gctgaactgg aacgcctcca gaaagaacgg
     3601 aaggagagga agaagaatgg aaaagcacat cacaatgagt ggccgtgaca aggtgacagt
     3661 tgatttccag gcgaaagacc tgaatcttgt ttacttcttc gggcagtgca aaagcacatt
     3721 caagtggatg acgagcagcg cacgcgcagt gcatgctgca gacaccgccg cgcagccgca
     3781 gacagggtgg cagcaaggag ccgactgacg gaggtggagt cccgctgtcg tgaaatcacg
     3841 ctgcagttca gcacacaatt gctatctatc catagaccat tcttgaccga gcaagcatgc
     3901 acgttacggg cagttacatt catcaagttt ttaaaaccaa ggggagactt gtatactggg
     3961 cctgtttttt tagcctgttt gctacctttt ttgcattcta tcccatgtga attttacaga
     4021 taccgggcta aaaaagggta tttggacact tggacacatt cctagaatgt catcctgtcc
     4081 tgttctacat caccaaatga cacagagaag accctgttta caactttttc tttctttctt
     4141 ttttttttaa gttcagatct ttctgaagag ataattctat atgacatatc tatagctatg
     4201 tgtatggcca tagatgtatt tctgtgtgta catatgtata gacatgtatt cctacatatg
     4261 tacatacaaa tgcagaggta tataaagtac ataggaattc cttacttgta aatagccaaa
     4321 aataaaaaat aaaaataaaa agtactgaca cgaatgatga attttcacat ttaaacagtc
     4381 accaacatga aagccatgtt taattgcttg tataatgtga tataatagag tttaaaataa
     4441 attatgcaca tggaatattt tca
//
