LOCUS       NM_001395725            5301 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus anoctamin 4 (Ano4), transcript variant 1, mRNA.
ACCESSION   NM_001395725 XM_008765231
VERSION     NM_001395725.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Suzuki,J., Fujii,T., Imao,T., Ishihara,K., Kuba,H. and Nagata,S.
  TITLE     Calcium-dependent phospholipid scramblase activity of TMEM16
            protein family members
  JOURNAL   J Biol Chem 288 (19), 13305-13316 (2013)
   PUBMED   23532839
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Tian,Y., Schreiber,R. and Kunzelmann,K.
  TITLE     Anoctamins are a family of Ca2+-activated Cl- channels
  JOURNAL   J Cell Sci 125 (Pt 21), 4991-4998 (2012)
   PUBMED   22946059
REFERENCE   3  (bases 1 to 5301)
  AUTHORS   Duran,C., Qu,Z., Osunkoya,A.O., Cui,Y. and Hartzell,H.C.
  TITLE     ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are
            intracellular proteins
  JOURNAL   Am J Physiol Cell Physiol 302 (3), C482-C493 (2012)
   PUBMED   22075693
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Aug 2, 2021 this sequence version replaced XM_008765231.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.38556.1,
                                           SRR26360194.2152213.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1478              JAXUCZ010000007.1  25538723-25540200   c
            1479-1673           JAXUCZ010000007.1  25430702-25430896   c
            1674-1778           JAXUCZ010000007.1  25391587-25391691   c
            1779-1915           JAXUCZ010000007.1  25382750-25382886   c
            1916-2074           JAXUCZ010000007.1  25379747-25379905   c
            2075-2175           JAXUCZ010000007.1  25350445-25350545   c
            2176-2220           JAXUCZ010000007.1  25346949-25346993   c
            2221-2352           JAXUCZ010000007.1  25329869-25330000   c
            2353-2459           JAXUCZ010000007.1  25299957-25300063   c
            2460-2515           JAXUCZ010000007.1  25292887-25292942   c
            2516-2637           JAXUCZ010000007.1  25290658-25290779   c
            2638-2772           JAXUCZ010000007.1  25288387-25288521   c
            2773-2869           JAXUCZ010000007.1  25287269-25287365   c
            2870-2930           JAXUCZ010000007.1  25280823-25280883   c
            2931-3013           JAXUCZ010000007.1  25270905-25270987   c
            3014-3154           JAXUCZ010000007.1  25261538-25261678   c
            3155-3319           JAXUCZ010000007.1  25259208-25259372   c
            3320-3356           JAXUCZ010000007.1  25247027-25247063   c
            3357-3468           JAXUCZ010000007.1  25244648-25244759   c
            3469-3526           JAXUCZ010000007.1  25243655-25243712   c
            3527-3624           JAXUCZ010000007.1  25243440-25243537   c
            3625-3767           JAXUCZ010000007.1  25241233-25241375   c
            3768-3920           JAXUCZ010000007.1  25235227-25235379   c
            3921-4068           JAXUCZ010000007.1  25233836-25233983   c
            4069-4188           JAXUCZ010000007.1  25228343-25228462   c
            4189-4294           JAXUCZ010000007.1  25222171-25222276   c
            4295-4490           JAXUCZ010000007.1  25219244-25219439   c
            4491-5301           JAXUCZ010000007.1  25216389-25217199   c
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q13"
     gene            1..5301
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /note="anoctamin 4"
                     /db_xref="GeneID:299714"
                     /db_xref="RGD:1310252"
     exon            1..1478
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1673
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     CDS             1619..4486
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /note="isoform 1 is encoded by transcript variant 1;
                     transmembrane protein 16D (eight membrane-spanning
                     domains); chromosome alignment-maintaining phosphoprotein
                     1-like"
                     /codon_start=1
                     /product="anoctamin-4 isoform 1"
                     /protein_id="NP_001382654.1"
                     /db_xref="GeneID:299714"
                     /db_xref="RGD:1310252"
                     /translation="MEASSSGITNGKNKVFHAEGGVDLQNHQLDMQTLSDGPKSDVDF
                     SEILNAIQEMAKDVNIFFDELEAVNSPCKDDDSLLHPGNLTSTSEDTSRLEAGGEKVR
                     ERNKSNGLYFRDGKCRIDYILVYRKSNPQTEKREVFERNIRAEGLQMEKESSLINSDI
                     IFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPM
                     RLDKETLPDLEENDCYTAPFSQQRIHHFIIHNKDTFFNNATRSRIVHHILQRIKYEEG
                     KNKIGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTHGAVNHRHLLYECWASWSVWYKYQ
                     PLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDHCQVSKEVCQAT
                     DIIMCPVCDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRA
                     VIAYDWDLIDWEEEEEEIRPQFEAKYSKKERMNPISGKPEPYQAFTDKCSRLIVSASG
                     IFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLN
                     VLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGA
                     YLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQ
                     EHGTERKINFPQWEKDYNLQPMNAYGLFDEYLEMILQFGFTTIFVAAFPLAPLLALLN
                     NIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPR
                     LVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFSGTPLKYC
                     RYRDYRDPPHSLAPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLR
                     DRMRREKYLIQEMMYEAELERLQKERKERKKNGKAHHNEWP"
     exon            1674..1778
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1915
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            1916..2074
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2175
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2176..2220
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2352
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2459
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2460..2515
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2516..2637
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2638..2772
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2773..2869
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2870..2930
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            2931..3013
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3014..3154
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3319
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3320..3356
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3357..3468
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3469..3526
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3527..3624
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3625..3767
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3768..3920
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            3921..4068
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            4069..4188
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            4189..4294
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            4295..4490
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
     exon            4491..5301
                     /gene="Ano4"
                     /gene_synonym="Tmem16d"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggtagtctat gtcagcctgc agtttgccca tggctggaag gcatcacaga acgtccatgg
       61 aattttcaac ctctaagaga aaagagaact ataattagaa actcagcatc acataaagac
      121 accaagggtc agtatcctct tactcctgac gggagaaagg aattggttct tctactaaga
      181 aagtaacctt aaacattctt ccaaagttaa aaaaaatctg gaagtctcac tatgacctct
      241 cttagtgttt agcctcagaa accactttct ggtccaattt aatcatcaca ggcaaacacg
      301 gtttaatatg aatgccaccg aatggtttta gaaaaagaga aatactaaaa gtgtagaatt
      361 atttttcatt aaccatggag gcctgggttc ctttctgttg gcgtaaactg tggtatgtca
      421 ctttagtgat tatagattat taatctctgt tcaccaagag taagcatgtc ctgtcttaaa
      481 tcgtgggtga tttaagagtc gtcaaagaga aacacaaacc gtccttagga actcacattg
      541 aacaacatct ctataaaaca ccacccagaa actcaatgaa gaaagaggga gggtcaaatt
      601 cccgaagaga cctttaactc ttttctgtct aaatttctcc catgatgtga aaagccctat
      661 tcctgacaat ctcaacctgc acttctgtca tagacaggca caggtccaca gtgccatttt
      721 cgaactggtg gtgaccttgt ggagggtcct gtcagagctg tctgatgtga gaggagtcac
      781 tagccaccac tcacattccc tctggcttcc tgcagagaga gggtggaaaa ctgccttggg
      841 aacttgctga gtggcccatg gaactatggg gctttgggaa ttaacaaaca gcaaacaaat
      901 attaagagac cgggagtaag caagctgcca atcctgccaa acttctgttg ctggcaggga
      961 gctgtcctgc agctccctgg tccgcctgtc atctgcactc tcttgtcaca ctgacaacag
     1021 ccccctggtt aggctgattg gttgctggca ataactgata tggaatactg atgtgcttac
     1081 cggcagttcc ttccactcca aagaggctcc ttccctaacc caaacctcgc tttccgaagc
     1141 ctagcctgct gcccccgccc cccaccccat gccgcccctg caccggcggg gaggaccagt
     1201 cctaggagcg atgctctgag attgtggtga aattggtccc cggctggctg ctttcactcc
     1261 ggctcggctc ccttgctctc cggggagagt cctgttccag acttctcggg gttttacagc
     1321 acagacaact gcatgcgggc tggaggggag gctgaaagga cttgtgtgtg gagggcttgt
     1381 tgcctcccat cactttgcca aggttcgctg tgaatttcct tctaccacag acaccggagg
     1441 aaggatctct tcttctgttg actctttcta tttttatggt ttaagtttat ctattcatgg
     1501 ggctgaaaag cgcttgcaaa tccatcaacg gcgaagtgtg gcaagccgcc cagggtcacg
     1561 acgtcgcctg cctgtggtca ggcattcctc actcccacca ggcagacggt caataaaaat
     1621 ggaggccagc tcttctggaa tcactaatgg aaaaaacaaa gttttccatg cagaaggtgg
     1681 agtggatttg caaaaccatc agctggatat gcaaacactg tccgacggcc caaagagcga
     1741 tgtggacttt tcagagattc ttaatgcaat acaagaaatg gccaaggatg tcaacatttt
     1801 ttttgatgaa ctagaggctg tcaacagccc gtgcaaagac gatgattctc tccttcaccc
     1861 tggaaatctg accagtacgt cggaggacac cagcagactg gaagcagggg gagagaaagt
     1921 gcgagaacga aacaaatcaa atggacttta ctttagagac ggaaaatgtc gaattgacta
     1981 catccttgtg tacagaaaat ccaaccctca gactgaaaag agagaagtgt ttgaaagaaa
     2041 tattagagca gaaggattac agatggagaa agagtcctct ctaataaata gtgacattat
     2101 ctttgtgaag ttgcatgccc catgggaagt ccttggaaga tatgcagagc aaatgaatgt
     2161 acggatgcct ttcaggagaa aaatctatta cctgccccgc cggtacaagt tcatgagcag
     2221 gatcgataaa caaataagca ggttccggag atggttacct aagaagccaa tgaggctgga
     2281 caaggagaca ctgccagacc tggaagagaa tgactgctac actgcccctt tcagccagca
     2341 aaggattcat cacttcatca tacacaacaa agacactttc ttcaacaacg ccaccagaag
     2401 cagaatcgtg catcatattt tacaaaggat aaagtacgag gaaggaaaaa acaaaatcgg
     2461 tctgaaccgc ttgcttacca atggctccta tgaagctgct tttcccctgc atgagggaag
     2521 ttatagaagt aaaaactcca ttaaaaccca tggagctgta aaccaccggc atctactgta
     2581 tgagtgctgg gcctcctgga gtgtgtggta caagtaccag cctttggatc ttgtaaggcg
     2641 gtattttgga gagaagattg gactgtattt tgcctggctg ggatggtaca caggcatgct
     2701 cttcccggca gccttcattg gcttgtttgt cttcttgtat ggagtcacca ctctggatca
     2761 ctgccaagtc agtaaagaag tctgccaagc aacagatatc atcatgtgtc ctgtgtgtga
     2821 caagtactgt ccattcatga ggctatcaga cagctgcgtc tatgccaagg tgacccacct
     2881 ttttgacaac ggggctactg tgttctttgc tgtgttcatg gcagtctggg ccactgtttt
     2941 cctggagttc tggaagagac gccgagcggt gatcgcttat gactgggatc tgattgactg
     3001 ggaagaagag gaggaagaaa tacggcccca gtttgaagcc aagtattcca agaaagagcg
     3061 gatgaatccc atttcgggaa agccagaacc ttatcaggca tttactgata aatgcagcag
     3121 acttatcgtt tctgcatcgg gaatattctt catgatctgt gtggtgatcg cggccgtgtt
     3181 cgggatcgtc atttaccggg tggtgaccgt gagcactttc gccgccttta agtgggcgtt
     3241 aatcaggaac aactctcagg tcgcgaccac agggactgca gtgtgcatca acttctgtat
     3301 cattatgctg ctgaacgtgc tctatgaaaa agtcgcactc cttctgacaa atctggaaca
     3361 gcctcgcaca gaatccgagt gggagaacag ctttaccttg aaaatgttcc tttttcagtt
     3421 cgtcaatctg aacagctcta ctttttacat cgcattcttc cttggaagat ttacaggaca
     3481 cccaggtgcc tacttgagat tgataaacag gtggaggcta gaagagtgcc accctagtgg
     3541 atgccttatt gatctctgca tgcaaatggg tattataatg gtgctaaagc agacctggaa
     3601 taatttcatg gaacttggct acccgttaat tcagaattgg tggactagaa gaaaagtccg
     3661 acaagaacat gggactgaaa ggaaaataaa tttcccacag tgggaaaagg actacaatct
     3721 tcagccgatg aatgcctatg gactcttcga tgaatacctg gaaatgatcc ttcagttcgg
     3781 attcacaact atctttgtgg cagcttttcc tctagcaccg cttctggcct tactgaataa
     3841 catcattgaa atccgacttg atgcttacaa gtttgtgact cagtggagaa ggcctctggc
     3901 ttcgagggcc aaggacatag gaatttggta tggaattctc gaaggcatcg ggattctctc
     3961 tgttattacg aacgcgtttg tcatagcaat aacatccgac ttcatccctc gcttagtata
     4021 tgcttataaa tatggaccgt gtgcaggcca aggagaagct gggcagaagt gcatggttgg
     4081 ctatgtaaat gccagcttgt ccgtgttccg aatttctgac tttgaaaacc ggtctgagcc
     4141 agagtctgat ggcagtgagt tctcaggaac tcctctgaag tactgcagat atcgggacta
     4201 ccgggacccg cctcattctt tggctcccta tggctacaca ctgcagttct ggcatgtcct
     4261 cgcagctcgg ctggctttca tcattgtgtt tgagcacctc gtgttttgta taaagcacct
     4321 catttcctat ctgataccag acctcccgaa agatctaagg gaccggatga ggagagagaa
     4381 gtacttgatc caggagatga tgtatgaggc tgaactggaa cgcctccaga aagaacggaa
     4441 ggagaggaag aagaatggaa aagcacatca caatgagtgg ccgtgacaag gtgacagttg
     4501 atttccaggc gaaagacctg aatcttgttt acttcttcgg gcagtgcaaa agcacattca
     4561 agtggatgac gagcagcgca cgcgcagtgc atgctgcaga caccgccgcg cagccgcaga
     4621 cagggtggca gcaaggagcc gactgacgga ggtggagtcc cgctgtcgtg aaatcacgct
     4681 gcagttcagc acacaattgc tatctatcca tagaccattc ttgaccgagc aagcatgcac
     4741 gttacgggca gttacattca tcaagttttt aaaaccaagg ggagacttgt atactgggcc
     4801 tgttttttta gcctgtttgc tacctttttt gcattctatc ccatgtgaat tttacagata
     4861 ccgggctaaa aaagggtatt tggacacttg gacacattcc tagaatgtca tcctgtcctg
     4921 ttctacatca ccaaatgaca cagagaagac cctgtttaca actttttctt tctttctttt
     4981 ttttttaagt tcagatcttt ctgaagagat aattctatat gacatatcta tagctatgtg
     5041 tatggccata gatgtatttc tgtgtgtaca tatgtataga catgtattcc tacatatgta
     5101 catacaaatg cagaggtata taaagtacat aggaattcct tacttgtaaa tagccaaaaa
     5161 taaaaaataa aaataaaaag tactgacacg aatgatgaat tttcacattt aaacagtcac
     5221 caacatgaaa gccatgttta attgcttgta taatgtgata taatagagtt taaaataaat
     5281 tatgcacatg gaatattttc a
//
