LOCUS       NM_001010970            1639 bp    mRNA    linear   ROD 17-JUL-2023
DEFINITION  Rattus norvegicus amylase alpha 1 (Amy1), mRNA.
ACCESSION   NM_001010970 XM_579333
VERSION     NM_001010970.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Mutlu A, Ozturk M, Akpinar G, Kasap M and Kanli A.
  TITLE     Proteomics analysis of pleomorphic adenoma of the human parotid
            gland
  JOURNAL   Eur Arch Otorhinolaryngol 274 (8), 3183-3195 (2017)
   PUBMED   28497265
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Chen,L.H., Yang,Z.M. and Chen,W.W.
  TITLE     Parallel increase in secretory activity between N-glycosylated and
            nonglycosylated alpha-amylase without protein synthesis after
            short-term beta-adrenergic receptor activation in isolated rat
            parotid acinar cells
  JOURNAL   Anal Quant Cytopathol Histpathol 36 (5), 279-284 (2014)
   PUBMED   25804000
  REMARK    GeneRIF: Our findings suggest a parallel increase in secretory
            activity between N-glycosylated and nonglycosylated a-amylase after
            b-adrenergic receptor activation.
REFERENCE   3  (bases 1 to 1639)
  AUTHORS   Archambaud C, Nahori MA, Soubigou G, Becavin C, Laval L, Lechat P,
            Smokvina T, Langella P, Lecuit M and Cossart P.
  TITLE     Impact of lactobacilli on orally acquired listeriosis
  JOURNAL   Proc Natl Acad Sci U S A 109 (41), 16684-16689 (2012)
   PUBMED   23012479
REFERENCE   4  (bases 1 to 1639)
  AUTHORS   Chang M, Alsaigh T, Kistler EB and Schmid-Schonbein GW.
  TITLE     Breakdown of mucin as barrier to digestive enzymes in the ischemic
            rat small intestine
  JOURNAL   PLoS One 7 (6), e40087 (2012)
   PUBMED   22768227
  REMARK    GeneRIF: These results indicate that mucin plays an important role
            in the preservation of the mucosal barrier and that ischemia but
            not digestive enzymes disturbs mucin integrity, while digestive
            enzymes actively mediate epithelial cell disruption.
REFERENCE   5  (bases 1 to 1639)
  AUTHORS   Imai A, Yoshie S, Ishibashi K, Haga-Tsujimura M, Nashida T,
            Shimomura H and Fukuda M.
  TITLE     EPI64 protein functions as a physiological GTPase-activating
            protein for Rab27 protein and regulates amylase release in rat
            parotid acinar cells
  JOURNAL   J Biol Chem 286 (39), 33854-33862 (2011)
   PUBMED   21832089
  REMARK    GeneRIF: EPI64 acted as a physiological Rab27-GAP that enhanced
            GTPase activity of Rab27 in response to IPR stimulation, and that
            this activity is required for IPR-induced amylase release.
REFERENCE   6  (bases 1 to 1639)
  AUTHORS   Venkatesh SG, Tan J, Gorr SU and Darling DS.
  TITLE     Isoproterenol increases sorting of parotid gland cargo proteins to
            the basolateral pathway
  JOURNAL   Am J Physiol Cell Physiol 293 (2), C558-C565 (2007)
   PUBMED   17537806
  REMARK    GeneRIF: Increased salivary amylase was detected in sera of
            isoproterenol-treated animals, suggesting increased routing of the
            regulated secretory proteins to the basolateral pathway.
REFERENCE   7  (bases 1 to 1639)
  AUTHORS   Youssef R, Malaisse WJ, Courtois P and Sener A.
  TITLE     Alteration of alpha-amylase secretion from rat parotid cells in the
            absence of extracellular bicarbonate
  JOURNAL   Int J Mol Med 12 (2), 199-200 (2003)
   PUBMED   12851717
  REMARK    GeneRIF: the HCO3-anion participates in the secretory sequence at
            sites distal to the identification of secretagogues in both
            endocrine and exocrine cells, the absence of extracellular
            bicarbonate is shown to decrease by about 50% basal alpha-amylase
            secretion
REFERENCE   8  (bases 1 to 1639)
  AUTHORS   Sierra,F., Pittet,A.C. and Schibler,U.
  TITLE     Different tissue-specific expression of the amylase gene Amy-1 in
            mice and rats
  JOURNAL   Mol Cell Biol 6 (11), 4067-4076 (1986)
   PUBMED   3025629
REFERENCE   9  (bases 1 to 1639)
  AUTHORS   Bloor,J.H. and Meisler,M.H.
  TITLE     Additional evidence for the close linkage of amy-1 and amy-2 in the
            mouse
  JOURNAL   J Hered 71 (6), 449-451 (1980)
   PUBMED   6163812
REFERENCE   10 (bases 1 to 1639)
  AUTHORS   MacDonald,R.J., Crerar,M.M., Swain,W.F., Pictet,R.L., Thomas,G. and
            Rutter,W.J.
  TITLE     Structure of a family of rat amylase genes
  JOURNAL   Nature 287 (5778), 117-122 (1980)
   PUBMED   6159531
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AB057450.1.
            
            On Apr 19, 2005 this sequence version replaced XM_579333.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB057450.1, FQ219213.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q42"
     gene            1..1639
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /note="amylase alpha 1"
                     /db_xref="GeneID:24203"
                     /db_xref="RGD:2113"
     exon            1..49
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..1612
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /EC_number="3.2.1.1"
                     /note="amylase 1, salivary; alpha-amylase 1; amylase,
                     alpha 1A (salivary)"
                     /codon_start=1
                     /product="alpha-amylase 1 precursor"
                     /protein_id="NP_001010970.1"
                     /db_xref="GeneID:24203"
                     /db_xref="RGD:2113"
                     /translation="MREYCQHHSRMKFFLLLSLIGLCWAQYDPHTFYGRSSIVHLFEW
                     RWVDIAKECERYLAPNGFGGVQVSPPNENIVVNSPFRPWWERYQPISYKICSRSGNED
                     EFRDMVNRCNNVGVRIYVDAVINHMCGVGAEAGQSSTCGSYFNPNNRDFPGVPYSGFD
                     FNDGKCKTGSGGIENYNDAAQVRDCRLSGLLDLALEKDYVRTKVADYMNHLIDIGVAG
                     FRLDASKHMWPGDIKAVLDKLHNLNTKWFSEGSKPFIYQEVIDLGGEAVSSNEYFGNG
                     RVTEFKYGAKLGKVLRKWDGEKMAYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGG
                     SSILTFWDARLYKMAVGFMLAHPYGFTRVMSSFHWPRYFENGKDVNDWVGPPNNNGAT
                     KEVTINSDSTCGNDWVCEHRWRQIRNMVAFRNVVNGQPFANWWDNGSNQVAFGRGNKG
                     FIVFNNDDWDLSTTLQTGLPAGTYCDVISGDKVDGNCTGIKVYVGSDGNAYFSISNSA
                     EDPFIAIHVESKI"
     exon            50..244
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            245..391
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            392..589
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            590..820
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            821..954
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1077
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1177
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1296
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1422
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1639
                     /gene="Amy1"
                     /gene_synonym="Amy1a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaaagctata ctctagattt ggatggttgc tggttgatac aagttgatga gagaatactg
       61 ccaacatcat agcagaatga aattcttcct gctgctttcc ctcattgggc tctgctgggc
      121 ccaatatgac ccacatactt tttatggacg gtcttctatt gtccatttgt tcgagtggcg
      181 ctgggttgat attgctaagg agtgtgagcg atatttagct cctaatggat ttggaggcgt
      241 gcaggtttct ccacccaatg aaaatattgt ggtcaacagt ccttttagac catggtggga
      301 aagatatcaa ccaattagct acaaaatatg ctccaggtct ggaaatgagg atgaattcag
      361 agacatggtg aacagatgca acaacgttgg tgtccgtatt tatgtggatg cggtcattaa
      421 tcacatgtgt ggggttgggg ctgaagctgg acaaagcagt acatgcggaa gttatttcaa
      481 cccaaataac agggactttc ctggagttcc gtattctggt ttcgacttta atgatgggaa
      541 atgtaaaact ggaagtggag gcattgagaa ctacaatgat gctgctcagg tcagagattg
      601 tcgtctgtct ggccttctgg atcttgcact tgagaaagat tatgttcgta ccaaggtggc
      661 tgattacatg aaccatctca ttgatattgg tgtagcaggg ttcagacttg atgcttctaa
      721 acacatgtgg cctggagaca taaaggcagt tttggacaaa ctacataatc taaatacaaa
      781 atggttctcc gaaggaagca aacctttcat ttatcaagag gtcattgatc tgggtggtga
      841 agcagtgtca agtaatgagt attttggaaa tggccgtgtg acagaattca aatatggagc
      901 aaaactgggc aaagttttgc gcaagtggga tggagaaaag atggcctact taaagaactg
      961 gggagaaggt tggggtttca tgccttctga cagagccctt gtctttgtgg acaaccatga
     1021 caaccagcga ggacatggtg ctggaggatc atccatcctg actttctggg atgctcgact
     1081 ctataaaatg gctgtcggat ttatgttggc tcatccttat ggtttcactc gggtaatgtc
     1141 aagtttccat tggccaagat atttcgagaa tggcaaagat gtcaatgact gggttggacc
     1201 acctaataac aatggagcaa ccaaagaagt gaccattaac tcagacagca cttgtggcaa
     1261 tgactgggtc tgtgaacatc gatggcgtca aataaggaac atggttgcct tcaggaatgt
     1321 ggtcaatggt cagccttttg caaactggtg ggataatggc agcaaccaag tagcttttgg
     1381 cagaggaaac aaaggcttca ttgtctttaa caatgatgac tgggacttgt caacaactct
     1441 acagactggt cttcctgctg gcacatactg tgatgtcatt tctggagaca aagtcgatgg
     1501 caattgcact ggaataaaag tctatgttgg cagtgatggc aatgcttact tctctattag
     1561 taactctgct gaagacccat tcattgcaat ccatgtagag tcaaaaatct aaaatttaaa
     1621 ataaacataa attgagagc
//
