LOCUS       NM_022217               3207 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Rattus norvegicus amphiphysin (Amph), mRNA.
ACCESSION   NM_022217 XM_001081729
VERSION     NM_022217.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Catsburg,L.A., Westra,M., van Schaik,A.M. and MacGillavry,H.D.
  TITLE     Dynamics and nanoscale organization of the postsynaptic endocytic
            zone at excitatory synapses
  JOURNAL   Elife 11, e74387 (2022)
   PUBMED   35072626
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Wuertenberger,S. and Groemping,Y.
  TITLE     A single PXXP motif in the C-terminal region of srGAP3 mediates
            binding to multiple SH3 domains
  JOURNAL   FEBS Lett 589 (10), 1156-1163 (2015)
   PUBMED   25819436
REFERENCE   3  (bases 1 to 3207)
  AUTHORS   Wilhelm,B.G., Mandad,S., Truckenbrodt,S., Krohnert,K., Schafer,C.,
            Rammner,B., Koo,S.J., Classen,G.A., Krauss,M., Haucke,V., Urlaub,H.
            and Rizzoli,S.O.
  TITLE     Composition of isolated synaptic boutons reveals the amounts of
            vesicle trafficking proteins
  JOURNAL   Science 344 (6187), 1023-1028 (2014)
   PUBMED   24876496
REFERENCE   4  (bases 1 to 3207)
  AUTHORS   Ritter,B., Murphy,S., Dokainish,H., Girard,M., Gudheti,M.V.,
            Kozlov,G., Halin,M., Philie,J., Jorgensen,E.M., Gehring,K. and
            McPherson,P.S.
  TITLE     NECAP 1 regulates AP-2 interactions to control vesicle size,
            number, and cargo during clathrin-mediated endocytosis
  JOURNAL   PLoS Biol 11 (10), e1001670 (2013)
   PUBMED   24130457
REFERENCE   5  (bases 1 to 3207)
  AUTHORS   Wahl,S., Katiyar,R. and Schmitz,F.
  TITLE     A local, periactive zone endocytic machinery at photoreceptor
            synapses in close vicinity to synaptic ribbons
  JOURNAL   J Neurosci 33 (25), 10278-10300 (2013)
   PUBMED   23785143
REFERENCE   6  (bases 1 to 3207)
  AUTHORS   Traub,L.M., Downs,M.A., Westrich,J.L. and Fremont,D.H.
  TITLE     Crystal structure of the alpha appendage of AP-2 reveals a
            recruitment platform for clathrin-coat assembly
  JOURNAL   Proc Natl Acad Sci U S A 96 (16), 8907-8912 (1999)
   PUBMED   10430869
REFERENCE   7  (bases 1 to 3207)
  AUTHORS   Slepnev,V.I., Ochoa,G.C., Butler,M.H., Grabs,D. and De Camilli,P.
  TITLE     Role of phosphorylation in regulation of the assembly of endocytic
            coat complexes
  JOURNAL   Science 281 (5378), 821-824 (1998)
   PUBMED   9694653
REFERENCE   8  (bases 1 to 3207)
  AUTHORS   Micheva,K.D., Kay,B.K. and McPherson,P.S.
  TITLE     Synaptojanin forms two separate complexes in the nerve terminal.
            Interactions with endophilin and amphiphysin
  JOURNAL   J Biol Chem 272 (43), 27239-27245 (1997)
   PUBMED   9341169
REFERENCE   9  (bases 1 to 3207)
  AUTHORS   Wigge,P., Kohler,K., Vallis,Y., Doyle,C.A., Owen,D., Hunt,S.P. and
            McMahon,H.T.
  TITLE     Amphiphysin heterodimers: potential role in clathrin-mediated
            endocytosis
  JOURNAL   Mol Biol Cell 8 (10), 2003-2015 (1997)
   PUBMED   9348539
REFERENCE   10 (bases 1 to 3207)
  AUTHORS   Lichte,B., Veh,R.W., Meyer,H.E. and Kilimann,M.W.
  TITLE     Amphiphysin, a novel protein associated with synaptic vesicles
  JOURNAL   EMBO J 11 (7), 2521-2530 (1992)
   PUBMED   1628617
  REMARK    Erratum:[EMBO J 1992 Oct;11(10):3809]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Sep 29, 2022 this sequence version replaced NM_022217.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643285.48824.1, Y13381.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               JAXUCZ010000017.1  50678357-50678501   c
            146-226             JAXUCZ010000017.1  50570584-50570664   c
            227-281             JAXUCZ010000017.1  50526291-50526345   c
            282-376             JAXUCZ010000017.1  50521113-50521207   c
            377-472             JAXUCZ010000017.1  50518750-50518845   c
            473-580             JAXUCZ010000017.1  50499665-50499772   c
            581-666             JAXUCZ010000017.1  50498461-50498546   c
            667-742             JAXUCZ010000017.1  50489397-50489472   c
            743-825             JAXUCZ010000017.1  50488736-50488818   c
            826-964             JAXUCZ010000017.1  50486458-50486596   c
            965-1093            JAXUCZ010000017.1  50485174-50485302   c
            1094-1210           JAXUCZ010000017.1  50476400-50476516   c
            1211-1234           JAXUCZ010000017.1  50473733-50473756   c
            1235-1258           JAXUCZ010000017.1  50471910-50471933   c
            1259-1291           JAXUCZ010000017.1  50469476-50469508   c
            1292-1336           JAXUCZ010000017.1  50468797-50468841   c
            1337-1459           JAXUCZ010000017.1  50464974-50465096   c
            1460-1654           JAXUCZ010000017.1  50450705-50450899   c
            1655-1918           JAXUCZ010000017.1  50449258-50449521   c
            1919-2020           JAXUCZ010000017.1  50446567-50446668   c
            2021-3207           JAXUCZ010000017.1  50435018-50436204   c
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q11"
     gene            1..3207
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /note="amphiphysin"
                     /db_xref="GeneID:60668"
                     /db_xref="RGD:620274"
     exon            1..145
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..2128
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /note="amphiphysin 1"
                     /codon_start=1
                     /product="amphiphysin"
                     /protein_id="NP_071553.2"
                     /db_xref="GeneID:60668"
                     /db_xref="RGD:620274"
                     /translation="MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQ
                     NFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKC
                     DVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKR
                     KDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVSSLEAKFHK
                     EIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEASPLPSPTAS
                     PNHTLAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELQQENIINFFEDNFVPEINV
                     TTPSQNEVLEVKKEETLLDLDFDPFKPDVTPAGSAAATHSPMSQTLPWDLWTTSTDLV
                     QPASGGSFNDFTQPQDTSLFTMQTDQNMAETEQALPTEPQAEEPPTTAAAPTAGLDLG
                     LEMEEPKEEAAIPPGTDAGETVGTEGSPGEEAEAEKAALPAGEGESPEGAKIDVESTE
                     LASSESPQAAELEAGAPQEKVIPSVVIEPASNHEGEEHQETTTGTETREATEDVAPQG
                     PAGEKQELATEPTPLDSQAATPAPAGAVDASLSAGDAAQELPPGFLYKVETLHDFEAA
                     NSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRR
                     E"
     exon            146..226
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            227..281
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            282..376
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            377..472
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            473..580
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            581..666
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            667..742
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            743..825
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..964
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1093
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1210
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1234
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1258
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1291
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1336
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1459
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1654
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1918
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2020
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..3207
                     /gene="Amph"
                     /gene_synonym="Amph1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctcttcctgg gctcctccgc gccgctgccg gctcgctgcg cgctctgctc ttccagctct
       61 gcagaccgca gcagccatgg ccgacatcaa gacgggcatc ttcgccaaaa acgtgcaaaa
      121 gcggctcaac cgggcacagg aaaaggtcct ccagaagctg ggaaaggcag atgaaacaaa
      181 agatgaacag tttgaagaat atgttcagaa cttcaagcgg caagaggcag agggtaccag
      241 acttcagcga gaactgcgag gatatttagc ggcaatcaaa ggtatgcagg aggcctcaat
      301 gaagcttacc gagtcactac atgaagtcta cgagccagat tggtatggac gggaagatgt
      361 aaagatggtt ggtgagaagt gtgatgtgct gtgggaagac ttccatcaaa agctagtgga
      421 tgggtccttg ctgacactgg acacatacct gggccagttt cctgacataa agaatcgcat
      481 cgctaagcgc agcaggaagc tcgtggatta cgacagtgcc cgccatcacc tggaggctct
      541 gcagagttcc aaaaggaagg atgagagtcg gatctctaag gcagaagagg agtttcagaa
      601 agcacagaaa gtgtttgaag agttcaatgt tgacttacaa gaagaattgc catcactatg
      661 gtcaagacga gttgggtttt atgtcaatac tttcaaaaac gtctctagcc ttgaggccaa
      721 gtttcacaag gaaatcgcag tgctttgcca caagctttat gaagtgatga cgaaacttgg
      781 tgatcagcac gctgacaagg ccttcagcat tcaaggagct cccagtgatt cgggtcctct
      841 tcgcattgca aagacaccat cacccccaga ggaggcttca cccctcccca gcccaacggc
      901 tagtcccaac cacacattag cacctgcatc tcctgcccca gtgcgaccca gatcaccttc
      961 acagacaagg aaagggcctc ctgtcccacc tctgcctaaa gtcaccccaa caaaggaact
     1021 acagcaggag aacatcatca atttctttga ggacaacttt gtaccagaaa tcaatgtgac
     1081 aacaccttcc cagaatgaag tcctggaggt gaagaaggag gagactttgc tggatttgga
     1141 ctttgaccct ttcaaacctg atgtgacccc agcaggttct gctgcagcga cccactcacc
     1201 catgtctcag acattgccct gggacttatg gacgacaagc actgatttgg tacaaccggc
     1261 ttctggaggt tcgtttaatg attttaccca gccccaggat acttcattat tcacgatgca
     1321 gacagatcag aatatggctg aaactgagca ggctctacct acagagcccc aggcagagga
     1381 gcctcccacc actgctgctg cacctactgc tgggctggac cttgggctag agatggagga
     1441 gccaaaggag gaggcagcga tccctcctgg aactgatgct ggtgagactg tgggaactga
     1501 ggggtcccca ggggaggaag cagaggcaga gaaggcagcc ctacctgctg gagaaggcga
     1561 aagtcctgag ggagccaaaa tcgatgtgga aagcactgag cttgcaagca gtgagagccc
     1621 tcaagcagca gagcttgaag ctggtgctcc tcaggaaaag gtcatccctt ctgttgtcat
     1681 agagccagcc tccaaccatg aaggagaaga acaccaagaa accactacgg gtactgaaac
     1741 aagggaggcc actgaggacg tggctcccca gggcccagcc ggtgagaagc aggaactggc
     1801 tacagagccc acgcctctgg attctcaggc tgctacgcct gcaccagcag gtgctgtgga
     1861 tgcttcgctg tctgcagggg atgccgctca ggaactgcct cctggctttc tctacaaggt
     1921 ggaaactctg catgacttcg aggcagcgaa ctctgatgaa cttaccctac aaaggggtga
     1981 tgtggtgctc gtggtgccct cagactcaga agccgaccag gatgcaggct ggttggtggg
     2041 ggtgaaggag tcagactggc ttcagtacag agacctggcc acatacaaag gcctctttcc
     2101 agagaacttc acacgacgcc gggagtaggg ctgcagatgt tgcaaaaggg tcatgtgact
     2161 gggtttttaa accttcatga aattcagagg agttcacttt tgctactaca ctcttaatga
     2221 ctgacagaca ggtgccagat gaggcttgag aagaccgatc aatagagcat gtatgcaaaa
     2281 gctataagag gggaaaaaac aaattaagga aagagtcctt actctgttcc ctgtgttcag
     2341 tagaggtctg tttgtgcttt gtctaaccat ggacactggt acttgattcc cctcccacca
     2401 aatctaaagt agctttctcc agaccagact ttgacttctt tgcatcaagt gtgtggtgtt
     2461 gagtgattgc cctgcagatg tgatgagtta tcctgtagta agacattatc ttactccctt
     2521 gcccgatgtg agtgagcacg tgctctcttt tcctttcaag tttactgcga ttaaaactga
     2581 acagctgcaa acgttcttaa cagtcttttc caatcacctc tatatacatc tatacggaca
     2641 cacagtcgca caagctagtt cccatgcctg ctggacctat gtgtatgcag atggctacat
     2701 atacatagct gattccttcc ctttccactg tagctcttcg ccttcgtcat tgatacacgc
     2761 attgctctta ttcgtggaca ttacagctgc agttttgcct tgaggcaaaa ccactcatcc
     2821 cattgccaaa acagttagtt gacagtgtct gtgtgtgttg tgtgcagttt tcaggatgat
     2881 ataacagata cagtgtctac gctgctgccc ctttggagag agcagggcct ggttgtatag
     2941 ctgcccagct gctcagtcca gtaaatccat ggaccagcaa ctatgcagtc atcatttctg
     3001 cctttatatg attggctgta tgagggggct aaagaaccta tttacaaaag ctcctatata
     3061 ctttcacatt ctatttatgc aactcatctt cacagatcat tctaaaatga acgaggtgat
     3121 gtatgctgta ccacaaacca tctgtaacag ttgtgttctc agtgctgtgt cattccaaat
     3181 aaatctttgg taatctccaa gttacaa
//
