LOCUS       NM_001013413            5068 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Rattus norvegicus alsin Rho guanine nucleotide exchange factor ALS2
            (Als2), mRNA.
ACCESSION   NM_001013413 XM_001061827 XM_001064519 XM_001064573 XM_343574
VERSION     NM_001013413.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Masutani,T., Taguchi,K., Kumanogoh,H., Nakamura,S. and Maekawa,S.
  TITLE     Molecular interaction of neurocalcin alpha with alsin (ALS2)
  JOURNAL   Neurosci Lett 438 (1), 26-28 (2008)
   PUBMED   18482800
  REMARK    GeneRIF: These results suggest that alsin binds cell membrane in a
            Ca(2+)-dependent manner through NCalpha and regulates membrane
            dynamics.
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Suzuki-Utsunomiya,K., Hadano,S., Otomo,A., Kunita,R., Mizumura,H.,
            Osuga,H. and Ikeda,J.E.
  TITLE     ALS2CL, a novel ALS2-interactor, modulates ALS2-mediated endosome
            dynamics
  JOURNAL   Biochem Biophys Res Commun 354 (2), 491-497 (2007)
   PUBMED   17239822
REFERENCE   3  (bases 1 to 5068)
  AUTHORS   Lai,C., Xie,C., McCormack,S.G., Chiang,H.C., Michalak,M.K., Lin,X.,
            Chandran,J., Shim,H., Shimoji,M., Cookson,M.R., Huganir,R.L.,
            Rothstein,J.D., Price,D.L., Wong,P.C., Martin,L.J., Zhu,J.J. and
            Cai,H.
  TITLE     Amyotrophic lateral sclerosis 2-deficiency leads to neuronal
            degeneration in amyotrophic lateral sclerosis through altered AMPA
            receptor trafficking
  JOURNAL   J Neurosci 26 (45), 11798-11806 (2006)
   PUBMED   17093100
REFERENCE   4  (bases 1 to 5068)
  AUTHORS   Jacquier,A., Buhler,E., Schafer,M.K., Bohl,D., Blanchard,S.,
            Beclin,C. and Haase,G.
  TITLE     Alsin/Rac1 signaling controls survival and growth of spinal
            motoneurons
  JOURNAL   Ann Neurol 60 (1), 105-117 (2006)
   PUBMED   16802292
REFERENCE   5  (bases 1 to 5068)
  AUTHORS   Devon,R.S., Orban,P.C., Gerrow,K., Barbieri,M.A., Schwab,C.,
            Cao,L.P., Helm,J.R., Bissada,N., Cruz-Aguado,R., Davidson,T.L.,
            Witmer,J., Metzler,M., Lam,C.K., Tetzlaff,W., Simpson,E.M.,
            McCaffery,J.M., El-Husseini,A.E., Leavitt,B.R. and Hayden,M.R.
  TITLE     Als2-deficient mice exhibit disturbances in endosome trafficking
            associated with motor behavioral abnormalities
  JOURNAL   Proc Natl Acad Sci U S A 103 (25), 9595-9600 (2006)
   PUBMED   16769894
REFERENCE   6  (bases 1 to 5068)
  AUTHORS   Bros,V., Shemilt,S.J., Cooper,J.D., Skaper,S.D., Leigh,P.N. and
            Gallo,J.M.
  TITLE     A novel somatodendritic marker defined by a peptide derived from
            the ALS2 protein
  JOURNAL   Neuroreport 15 (14), 2155-2159 (2004)
   PUBMED   15371724
REFERENCE   7  (bases 1 to 5068)
  AUTHORS   Kunita,R., Otomo,A., Mizumura,H., Suzuki,K., Showguchi-Miyata,J.,
            Yanagisawa,Y., Hadano,S. and Ikeda,J.E.
  TITLE     Homo-oligomerization of ALS2 through its unique carboxyl-terminal
            regions is essential for the ALS2-associated Rab5 guanine
            nucleotide exchange activity and its regulatory function on
            endosome trafficking
  JOURNAL   J Biol Chem 279 (37), 38626-38635 (2004)
   PUBMED   15247254
REFERENCE   8  (bases 1 to 5068)
  AUTHORS   Topp,J.D., Gray,N.W., Gerard,R.D. and Horazdovsky,B.F.
  TITLE     Alsin is a Rab5 and Rac1 guanine nucleotide exchange factor
  JOURNAL   J Biol Chem 279 (23), 24612-24623 (2004)
   PUBMED   15033976
  REMARK    GeneRIF: Als2 gene encodes a large protein termed alsin, which
            contains a number of predicted cell signaling and protein
            trafficking sequence motifs.
REFERENCE   9  (bases 1 to 5068)
  AUTHORS   Otomo,A., Hadano,S., Okada,T., Mizumura,H., Kunita,R.,
            Nishijima,H., Showguchi-Miyata,J., Yanagisawa,Y., Kohiki,E.,
            Suga,E., Yasuda,M., Osuga,H., Nishimoto,T., Narumiya,S. and
            Ikeda,J.E.
  TITLE     ALS2, a novel guanine nucleotide exchange factor for the small
            GTPase Rab5, is implicated in endosomal dynamics
  JOURNAL   Hum Mol Genet 12 (14), 1671-1687 (2003)
   PUBMED   12837691
REFERENCE   10 (bases 1 to 5068)
  AUTHORS   Yang,Y., Hentati,A., Deng,H.X., Dabbagh,O., Sasaki,T., Hirano,M.,
            Hung,W.Y., Ouahchi,K., Yan,J., Azim,A.C., Cole,N., Gascon,G.,
            Yagmour,A., Ben-Hamida,M., Pericak-Vance,M., Hentati,F. and
            Siddique,T.
  TITLE     The gene encoding alsin, a protein with three guanine-nucleotide
            exchange factor domains, is mutated in a form of recessive
            amyotrophic lateral sclerosis
  JOURNAL   Nat Genet 29 (2), 160-165 (2001)
   PUBMED   11586297
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BK005190.1.
            
            On or before Feb 3, 2007 this sequence version replaced
            XM_343574.3, XM_001061827.1, XM_001064519.1, XM_001064573.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK005190.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q31"
     gene            1..5068
                     /gene="Als2"
                     /note="alsin Rho guanine nucleotide exchange factor ALS2"
                     /db_xref="GeneID:363235"
                     /db_xref="RGD:1310372"
     exon            1..54
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            55..132
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..5068
                     /gene="Als2"
                     /note="amyotrophic lateral sclerosis protein 2 homolog;
                     ALS2, alsin Rho guanine nucleotide exchange factor;
                     amyotrophic lateral sclerosis 2 protein homolog;
                     amyotrophic lateral sclerosis 2 (juvenile)"
                     /codon_start=1
                     /product="alsin"
                     /protein_id="NP_001013431.1"
                     /db_xref="GeneID:363235"
                     /db_xref="RGD:1310372"
                     /translation="MDSKKKSSAEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVL
                     QAALGVKHGVLLTEDGEVYSFGTLPWKSEAAEICPSSPLLESALVGHHVVTVATGSFH
                     SGAVTESGVVYMWGENAAGQCAVANQQYVSEPSPVSISDSETSPLLAVRILQLACGEE
                     HTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGAFHSLALVQ
                     CLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASTVT
                     SPHPETLDGQGEVFENTVAEAELNMGSDQTTSGSAISAQQNIVGMAEVSSARTAPSYP
                     DTQTVTAYLQKLSEHSVKENHEREEKLPQVQPLVEEAVPDLHSPPTTSTSALNSLVVS
                     CASAVGVRVAATYEAGALSLKKVMNFYSTAPCEPGAPSGTASTGPESLKDLREEQVKQ
                     ESLQGKKSSSLMDIREEESEGGSRRLSLPGLLSQVSPRLLRKAARVKTRTVVLTPTYS
                     GEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEAGGSHSL
                     ALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSESGVWSVAAGHGYSLFLVDTED
                     FQPGLYYSGRQDRAEGDTLPENPSGTTTPVLLSCSKLGYISRVTAGKDSYLALVDKNI
                     MGYIASLHELATTERRFYSKLSEIKSQILRPLLSLEHLGTVTTVQLLQEVASRFSKLC
                     YLIGQHGASLSSYLQGMKEARNLVIMKHSSLFLDSYTEYCTSVSNFLVMGGSQLLAKP
                     AIDFLNKNQELLQDLSEVNDENTQLMEILNALFFLPIRRLHNYAKVLLKLATCFEVTS
                     PEYQKLQDSSSCYESLALHLGKKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLCESSN
                     RALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEETGGVNGLKITT
                     PEEQFTLISSTPQEKTKWLRAISQAVDQALRGTSDFPLYGGSSTVQRQEPPISRSAKY
                     TFYKDTRLKDATYDGRWLSGKPHGRGVLKWPDGKVYSGTFRNGLEDGYGEYRIPNKAL
                     NKEDHYVGHWKEGKMCGQGVYSYASGEVFEGCFQDNMRHGHGLLRSGKLTSSSPSMFI
                     GQWVMDKKAGYGVFDDITRGEKYMGMWQDDACQGNGVVVTQFGLYYEGNFHLNKMMGN
                     GVLLSEDDTIYEGEFSDDWTLCGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKPSL
                     YESDKDRPKAFRKLGNLAVAADEKWRAVFDECWRQLGCESPGQGEVWKAWDNIAVALT
                     TNRRQHKDSPEILSRSQTQTLESLEYIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLG
                     RLVETLVAVYRMTYVGVGANRRLLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTVPL
                     SAPLPTGRRSFCTGKSDSRSESPEPGYVVTSSGLLLPVLLPRLYPPLFMLYALDNDRE
                     EDIYWECVLRLNKQPDIALLGFLGVQRKFWPATLSILGESKKVLPSTKDACFASAVEC
                     LQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRN
                     LGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN"
     exon            133..287
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            288..1207
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1565
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1734
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1831
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1909
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2092
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2264
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            2265..2445
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            2446..2511
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2674
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            2675..2806
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..2935
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3006
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3007..3073
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3074..3276
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3342
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3343..3441
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3442..3606
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3607..3718
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3719..3796
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3797..3930
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            3931..4098
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            4099..4216
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            4217..4374
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            4375..4497
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            4498..4674
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            4675..4720
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            4721..4782
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            4783..4932
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            4933..5029
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
     exon            5030..5068
                     /gene="Als2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggtgcagt cgggctcgcg ccgggcgaag agcgcggagc tgcgggagcg tcaggttttg
       61 agagagcttc tgctaatgga atgatttggt gatggagtat tcctcctgac cgatggactc
      121 aaagaagaaa agctcagcag aggcagaagg atccaaagaa agaggcctag tccatgtctg
      181 gcaggcagga tccttttctc taacaccaga gaggttgcca ggctggggag gaaaaacagt
      241 tcttcaggcg gcccttggcg tgaagcatgg agttcttctg actgaagatg gtgaggtcta
      301 cagctttggg acccttccct ggaaaagtga agcagcagaa atttgtccaa gcagccccct
      361 tctagaaagt gccctggttg ggcatcatgt tgttactgtg gcaacaggga gcttccacag
      421 tggagcggtg acagaaagtg gagtggtgta catgtggggg gagaacgctg ccgggcagtg
      481 tgcagtagcg aaccagcagt atgtgtcgga accaagtcct gtcagcattt ctgactcgga
      541 gaccagcccc ttgctagcag ttaggattct gcagttggca tgtggcgagg aacacacact
      601 ggcattgtca atcagcagag agatctgggc atggggcacc ggctgtcagc tgggtctcat
      661 caccaccacc ttcccagtga caaagccaca aaaggtggaa caccttgctg gacgagtggt
      721 gctccaggtg gcctgtggtg cgttccacag ccttgcactt gtgcagtgcc tccctcccca
      781 ggatctgaag ccagtcccag agagatgcaa tcagtgcagc cagctgctca tcaccatgac
      841 agacaaagag gaccacgtga taatatcaga cagccattgc tgccctttag gtgtgacact
      901 gtctgaatct caagcagaaa agcacgccag cactgtgacc agccctcacc cggagacgct
      961 ggatgggcaa ggagaggtgt ttgagaacac agtagcagaa gctgaactga atatgggaag
     1021 cgatcagacc acaagtggca gtgccatttc cgcccagcag aacatcgtgg gaatggctga
     1081 agtgtcttct gccagaacag ctccctcata cccagacacc cagacggtaa ctgcatacct
     1141 gcagaagcta tcagagcatt cagtgaagga gaaccatgag cgtgaggaaa agctacccca
     1201 ggtccagcct cttgtagaag aagcagttcc tgatcttcac agtccaccaa ccacaagcac
     1261 ctcagccctc aacagcttgg tggtctcctg tgcatctgct gttggtgtga gagtggccgc
     1321 cacctatgaa gctggggcct tgtctctcaa gaaagttatg aacttttata gcactgcccc
     1381 ctgcgagcca ggagctccgt caggcactgc ctccacaggc ccagaaagtc tgaaagatct
     1441 cagagaagag caggtgaaac aggaatcact acaagggaag aaaagctcaa gcctcatgga
     1501 tatcagagag gaggagtcgg agggagggag tcgaagactc tccctcccag gattgttgtc
     1561 gcaagtttcc cccaggctct taaggaaggc cgccagggtg aaaacacgga cagtggttct
     1621 gactcccacg tacagtggag aagcagatgc cctcctgcct tcgctgagaa cagaggtgtg
     1681 gacctggggg aaaggcaagg aagggcagct ggggcacggc gatgtcctgc ccaggcttca
     1741 gccgttgtgt gtcaagtgtc tggatggtaa agaggtcatc cacttggagg caggtggctc
     1801 ccactctctc gcactgactg cgaaatctca ggtttactca tggggcagta ataccttcgg
     1861 tcagctgggg cattctgagt ttccaacaac ggttcctcga ctctcgaagg ttagcagtga
     1921 aagtggagtc tggagtgtag ctgcaggcca cggttattcc ctgtttttag tggacactga
     1981 agacttccag cctgggttgt attacagtgg ccgccaggac cgtgcagaag gtgacaccct
     2041 gccagagaat cccagtggta caacgactcc ggtacttctc tcctgtagta agcttggata
     2101 tatcagcaga gtgacagcag gaaaagatag ctacctagcc ttggtggata agaacatcat
     2161 gggatacatc gccagcctcc atgagttggc taccacagaa agacggtttt actcgaaact
     2221 aagtgaaatc aagtcacaga tacttaggcc tcttctcagt ctggaacatt tgggcactgt
     2281 gaccactgtc cagctgttgc aggaagtcgc cagccggttc agcaagttgt gttacctcat
     2341 tgggcagcat ggagcctcac tgagcagcta cctgcaaggc atgaaggaag ccaggaacct
     2401 ggtcatcatg aagcactcga gtcttttctt ggacagttac acagagtatt gtacatcagt
     2461 ttcaaatttt ctggttatgg gaggatccca gcttcttgct aagcctgcaa ttgatttcct
     2521 aaataaaaac caagaactct tgcaagattt gtcagaagtg aatgatgaga acactcagtt
     2581 gatggaaata ctgaatgcgc tgtttttctt gccaatcaga cgacttcata attatgcaaa
     2641 agttttgcta aagctcgcca cttgctttga agtgacatca ccagagtatc aaaagctgca
     2701 ggattccagt tcttgttatg agtctcttgc tctccatctt ggcaagaaga ggaaggaagc
     2761 agagtacacg ctgagcttct ggaagacctt ccctggaaaa atgacggatt ccttgaggaa
     2821 gccagagcgc cggctgctgt gtgagagcag taaccgagcc ctctccctgc agcatgctgg
     2881 caggttttct gtgaattggt tcattctctt caatgatgcc ctggtccatg ctcagttctc
     2941 cacacaccac gtgttccctt tggccacact ctgggcagag ccactttctg aagaaactgg
     3001 tggcgtgaat ggcttaaaga taaccacacc tgaagaacag ttcacgctca tttcttcaac
     3061 accccaggaa aagaccaagt ggcttcgggc tatcagccaa gccgtggacc aggctttgag
     3121 ggggacgtct gacttcccac tttacggagg cagcagcact gttcagaggc aggaaccacc
     3181 catctcaaga agtgccaaat acactttcta caaggatact cgcctaaagg acgccactta
     3241 cgatgggcgc tggctttcag ggaagccgca cggaaggggt gttttgaagt ggcctgatgg
     3301 gaaggtgtat tctggcacgt tcaggaatgg cttggaagac ggatatggtg aatacagaat
     3361 cccaaacaag gccctgaaca aagaagacca ttacgtgggc cattggaagg aggggaaaat
     3421 gtgtgggcaa ggagtgtaca gctatgcctc tggtgaagtg tttgaaggct gctttcaaga
     3481 taacatgcgc cacgggcacg gtctcctccg cagtggaaaa ctgacttctt cctctcccag
     3541 catgttcatt ggccagtggg taatggacaa gaaagcaggg tatggcgtct tcgatgatat
     3601 caccagggga gaaaagtaca tgggaatgtg gcaggacgat gcatgccaag ggaatggggt
     3661 ggtggtcacc cagtttgggt tatactacga aggcaacttc caccttaata aaatgatggg
     3721 aaatggggtt ttgctttcgg aagatgatac tatatatgaa ggagaatttt ctgatgactg
     3781 gacactttgt ggaaagggaa cactgactat gccaaatgga gattatattg aaggttattt
     3841 tagtggagaa tggggatctg ggataaaaat cactgggacc tacttcaaac ctagcctata
     3901 tgagagtgat aaggacaggc ccaaagcctt caggaagctg ggaaacctgg ccgtggccgc
     3961 cgatgagaaa tggagagcag tgtttgacga atgctggcgc cagctgggct gtgagagccc
     4021 aggccaaggg gaggtttgga aagcgtggga taatattgct gtggccttga ccaccaaccg
     4081 tcgccagcat aaagacagtc cagaaatact aagccgttca cagactcaga ccctggagag
     4141 tttggagtac attccccagc acgttggcgc cttctctgtg gagaaatatg atgatatcaa
     4201 gaagtattta ataaaggcct gtgacactcc tctgcaccca ctgggcaggc ttgtggagac
     4261 cctggttgca gtgtatagaa tgacttatgt gggcgtaggg gccaaccgtc ggttactgca
     4321 ggaagctgtg aaggagatta agtcttatct caagaggatt ttccaacttg tgaggttctt
     4381 gtttcctgag cttcctgaag agggcagcac agttcctctc tctgctcctc tgcccactgg
     4441 aagaagatcc ttctgcactg ggaaatcaga ttccagatcc gagtcaccag agccaggtta
     4501 tgtagtaaca agttctggct tactgcttcc ggtgctgctg cctcggctct acccgcctct
     4561 cttcatgctc tatgccctgg acaatgaccg agaggaagac atttactggg aatgtgtgct
     4621 tcgactaaac aagcagccag atattgctct cctgggcttt cttggagtac agaggaaatt
     4681 ctggccagca accttgtcaa tccttggaga gagtaaaaag gtactgccat ccacaaagga
     4741 tgcttgtttt gcatctgcag tagaatgtct gcagcagatc agcacaacat ttactccatc
     4801 agacaaactt aaagtgatcc agcagacctt cgaagaaatc tctcagagtg tgctggcgtc
     4861 gctgcaggag gacttccttt ggtccatgga tgacttgttc cctgtcttct tatacgtggt
     4921 gctgcgggcc aggattcgga atttaggctc tgaagttcat ctcattgagg atctgatgga
     4981 cccctatctc cagcatgggg agcaaggcat aatgttcacc accttgaagg cctgttacta
     5041 ccagattcag cgtgagaagc ttaactag
//
