LOCUS       NP_037191                524 aa            linear   ROD 14-FEB-2025
DEFINITION  alkaline phosphatase, tissue-nonspecific isozyme precursor [Rattus
            norvegicus].
ACCESSION   NP_037191 NP_599169
VERSION     NP_037191.2
DBSOURCE    REFSEQ: accession NM_013059.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 524)
  AUTHORS   Brito,V.G.B., Patrocinio,M.S., de Sousa,M.C.L., Barreto,A.E.A.,
            Frasnelli,S.C.T., Lara,V.S., Santos,C.F. and Oliveira,S.H.P.
  TITLE     Telmisartan Prevents Alveolar Bone Loss by Decreasing the
            Expression of Osteoclasts Markers in Hypertensive Rats With
            Periodontal Disease
  JOURNAL   Front Pharmacol 11, 579926 (2020)
   PUBMED   33364953
  REMARK    Erratum:[Front Pharmacol. 2021 Feb 19;11:635927. doi:
            10.3389/fphar.2020.635927.. PMID: 33679393]
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 524)
  AUTHORS   Favarin,B.Z., Bolean,M., Ramos,A.P., Magrini,A., Rosato,N.,
            Millan,J.L., Bottini,M., Costa-Filho,A.J. and Ciancaglini,P.
  TITLE     Lipid composition modulates ATP hydrolysis and calcium phosphate
            mineral propagation by TNAP-harboring proteoliposomes
  JOURNAL   Arch Biochem Biophys 691, 108482 (2020)
   PUBMED   32710882
REFERENCE   3  (residues 1 to 524)
  AUTHORS   Gao,L., Wang,L.Y., Liu,Z.Q., Jiang,D., Wu,S.Y., Guo,Y.Q., Tao,H.M.,
            Sun,M., You,L.N., Qin,S., Cheng,X.C., Xie,J.S., Chang,G.L. and
            Zhang,D.Y.
  TITLE     TNAP inhibition attenuates cardiac fibrosis induced by myocardial
            infarction through deactivating TGF-beta1/Smads and activating P53
            signaling pathways
  JOURNAL   Cell Death Dis 11 (1), 44 (2020)
   PUBMED   31969558
  REMARK    GeneRIF: TNAP inhibition attenuates cardiac fibrosis induced by
            myocardial infarction through deactivating TGF-beta1/Smads and
            activating P53 signaling pathways.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 524)
  AUTHORS   Zhang,F., Sun,X., Yu,H., Yang,X., Song,M., Han,Y., Li,Y. and Zhu,Y.
  TITLE     Effects of aluminum trichloride on the cartilage stimulatory growth
            factors in rats
  JOURNAL   Biometals 30 (1), 143-150 (2017)
   PUBMED   27885436
  REMARK    GeneRIF: he rats body weight were decreased, the cartilage
            histological structure were disrupted, the cartilage and serum
            contents of Al and the serum level of C-telopeptide of type II
            collagen were all increased, the serum level of type II collagen
            (Col II) and alkaline phosphatase (ALP), and the mRNA expressions
            of TGF-beta1, BMP-2, ALP and Col II were all decreased in the
            AlCl3-treated groups compared with those in control g...
REFERENCE   5  (residues 1 to 524)
  AUTHORS   Kazemi,S., Mousavi Kani,S.N., Ghasemi-Kasman,M., Aghapour,F.,
            Khorasani,H. and Moghadamnia,A.A.
  TITLE     Nonylphenol induces liver toxicity and oxidative stress in rat
  JOURNAL   Biochem Biophys Res Commun 479 (1), 17-21 (2016)
   PUBMED   27590577
  REMARK    GeneRIF: Data show that nonylphenol (NP) increased serum alkaline
            phosphatase, lead to liver damage and can increase the expression
            of heme oxygenase-1 (HO1) and growth arrest and
            DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify
            the toxic effects on liver through induction of oxidative stress.
REFERENCE   6  (residues 1 to 524)
  AUTHORS   Zernik,J., Kream,B. and Twarog,K.
  TITLE     Tissue-specific and dexamethasone-inducible expression of alkaline
            phosphatase from alternative promoters of the rat
            bone/liver/kidney/placenta gene
  JOURNAL   Biochem Biophys Res Commun 176 (3), 1149-1156 (1991)
   PUBMED   2039500
REFERENCE   7  (residues 1 to 524)
  AUTHORS   Hahnel,A.C., Rappolee,D.A., Millan,J.L., Manes,T., Ziomek,C.A.,
            Theodosiou,N.G., Werb,Z., Pedersen,R.A. and Schultz,G.A.
  TITLE     Two alkaline phosphatase genes are expressed during early
            development in the mouse embryo
  JOURNAL   Development 110 (2), 555-564 (1990)
   PUBMED   2133555
REFERENCE   8  (residues 1 to 524)
  AUTHORS   Toh,Y., Yamamoto,M., Endo,H., Misumi,Y. and Ikehara,Y.
  TITLE     Isolation and characterization of a rat liver alkaline phosphatase
            gene. A single gene with two promoters
  JOURNAL   Eur J Biochem 182 (2), 231-237 (1989)
   PUBMED   2544423
REFERENCE   9  (residues 1 to 524)
  AUTHORS   Misumi,Y., Tashiro,K., Hattori,M., Sakaki,Y. and Ikehara,Y.
  TITLE     Primary structure of rat liver alkaline phosphatase deduced from
            its cDNA
  JOURNAL   Biochem J 249 (3), 661-668 (1988)
   PUBMED   2895632
REFERENCE   10 (residues 1 to 524)
  AUTHORS   Ogata,S., Hayashi,Y., Yasutake,K. and Ikehara,Y.
  TITLE     Chemical identification of lipid components in the membranous form
            of rat liver alkaline phosphatase
  JOURNAL   J Biochem 102 (6), 1609-1615 (1987)
   PUBMED   2834351
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Nov 26, 2020 this sequence version replaced NP_037191.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643294.23337.1, BC088399.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676787 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     Protein         1..524
                     /product="alkaline phosphatase, tissue-nonspecific isozyme
                     precursor"
                     /EC_number="3.1.3.1"
                     /EC_number="3.9.1.1"
                     /note="alkaline phosphatase 2, liver; alkaline
                     phosphatase, tissue-nonspecific isozyme; alkaline
                     phosphatase liver/bone/kidney isozyme; Tissue-nonspecific
                     ALP alkaline phosphatase; tissue-nonspecific alkaline
                     phosphatase; phosphoamidase; phosphocreatine phosphatase;
                     alkaline phosphatase, liver/bone/kidney"
                     /calculated_mol_wt=55871
     sig_peptide     1..17
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=1806
     mat_peptide     18..501
                     /product="Alkaline phosphatase, tissue-nonspecific
                     isozyme. /id=PRO_0000024027"
                     /note="propagated from UniProtKB/Swiss-Prot (P08289.2)"
                     /calculated_mol_wt=53521
     Region          51..490
                     /region_name="Alk_phosphatase"
                     /note="Alkaline phosphatase; pfam00245"
                     /db_xref="CDD:395188"
     Site            order(60,109..110,173,184,332,337,341,378..379,454)
                     /site_type="active"
                     /db_xref="CDD:293736"
     Site            order(60,109..110,184,337,341,379,454)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:293736"
     Site            order(64..65,68,71..72,95..97,99,101,103,107,120..122,132,
                     147,453,455..458,460,470,472..476,478..479,482)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:293736"
     Site            110
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P08289.2)"
     Site            140
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P08289.2)"
     Site            230
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P08289.2)"
     Site            271
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P08289.2)"
     Site            303
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P08289.2)"
     Site            430
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P08289.2)"
     CDS             1..524
                     /gene="Alpl"
                     /gene_synonym="Akp2; AP-TNAP; PHOA; TNAP; TNSALP"
                     /coded_by="NM_013059.3:168..1742"
                     /db_xref="GeneID:25586"
                     /db_xref="RGD:2100"
ORIGIN      
        1 milpflvlai gtcltnsfvp ekekdpsywr qqaqetlkna lklqklntnv akniimflgd
       61 gmgvstvtaa rilkgqlhhn tgeetrlemd kfpfvalskt yntnaqvpds agtataylcg
      121 vkanegtvgv saatertrcn ttqgnevtsi lrwakdagks vgivtttrvn hatpsaayah
      181 sadrdwysdn emppealsqg ckdiayqlmh nikdidvimg ggrkymypkn rtdveyelde
      241 kargtrldgl dlisiwksfk prhkhshyvw nrtellaldp srvdyllglf epgdmqyeln
      301 rnnltdpsls emvevalril tknpkgffll veggridhgh hegkakqalh eavemdeaig
      361 kagtmtsqkd tltvvtadhs hvftfggytp rgnsifglap mvsdtdkkpf tailygngpg
      421 ykvvdgeren vsmvdyahnn yqaqsavplr hethggedva vfakgpmahl lhgvheqnyi
      481 phvmayasci ganldhcawa ssasspspga lllplalfpl rtlf
//
