LOCUS       NM_022665               2226 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus alkaline phosphatase, intestinal (Alpi), mRNA.
ACCESSION   NM_022665
VERSION     NM_022665.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Rentea,R.M., Rentea,M.J., Biesterveld,B., Liedel,J.L. and
            Gourlay,D.M.
  TITLE     Factors Known to Influence the Development of Necrotizing
            Enterocolitis to Modify Expression and Activity of Intestinal
            Alkaline Phosphatase in a Newborn Neonatal Rat Model
  JOURNAL   Eur J Pediatr Surg 29 (3), 290-297 (2019)
   PUBMED   29723880
  REMARK    GeneRIF: Prematurity and formula feeding are associated with
            inhibition of intestinal alkaline phosphatase expression and
            activity. Both may increase the risk of necrotizing enterocolitis.
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Parlato,M., Charbit-Henrion,F., Pan,J., Romano,C.,
            Duclaux-Loras,R., Le Du,M.H., Warner,N., Francalanci,P.,
            Bruneau,J., Bras,M., Zarhrate,M., Begue,B., Guegan,N., Rakotobe,S.,
            Kapel,N., De Angelis,P., Griffiths,A.M., Fiedler,K., Crowley,E.,
            Ruemmele,F., Muise,A.M. and Cerf-Bensussan,N.
  TITLE     Human ALPI deficiency causes inflammatory bowel disease and
            highlights a key mechanism of gut homeostasis
  JOURNAL   EMBO Mol Med 10 (4) (2018)
   PUBMED   29567797
REFERENCE   3  (bases 1 to 2226)
  AUTHORS   Fawley,J., Koehler,S., Cabrera,S., Lam,V., Fredrich,K., Hessner,M.,
            Salzman,N. and Gourlay,D.
  TITLE     Intestinal alkaline phosphatase deficiency leads to dysbiosis and
            bacterial translocation in the newborn intestine
  JOURNAL   J Surg Res 218, 35-42 (2017)
   PUBMED   28985873
  REMARK    GeneRIF: Deficiency of IAP in the newborn rat intestine is
            associated with dysbiosis and increased inflammation, permeability,
            and bacterial translocation.
REFERENCE   4  (bases 1 to 2226)
  AUTHORS   Okazaki,Y. and Katayama,T.
  TITLE     Glucomannan consumption elevates colonic alkaline phosphatase
            activity by up-regulating the expression of IAP-I, which is
            associated with increased production of protective factors for gut
            epithelial homeostasis in high-fat diet-fed rats
  JOURNAL   Nutr Res 43, 43-50 (2017)
   PUBMED   28739053
  REMARK    GeneRIF: The present study showed that dietary glucomannan elevates
            colonic ALP activity by up-regulation of the expression of IAP-I
            which is associated with increased production of protective factors
            for gut epithelial homeostasis in high-fat diet-fed rats.
REFERENCE   5  (bases 1 to 2226)
  AUTHORS   Gomes,J.R., Ayub,L.C., Dos Reis,C.A., Machado,M.J., da Silva,J.,
            Omar,N.F. and de Miranda Soares,M.A.
  TITLE     Goblet cells and intestinal Alkaline phosphatase expression (IAP)
            during the development of the rat small intestine
  JOURNAL   Acta Histochem 119 (1), 71-77 (2017)
   PUBMED   27939968
  REMARK    GeneRIF: A temporal and spatial distribution of goblet cells and
            intestinal alkaline phosphatase activity occurs during the
            development of the small intestine, suggesting a possible
            regulatory control in accordance with the suckling and weaning
            phases of food intake in the rat's life.
REFERENCE   6  (bases 1 to 2226)
  AUTHORS   Harada,T., Koyama,I., Matsunaga,T., Kikuno,A., Kasahara,T.,
            Hassimoto,M., Alpers,D.H. and Komoda,T.
  TITLE     Characterization of structural and catalytic differences in rat
            intestinal alkaline phosphatase isozymes
  JOURNAL   FEBS J 272 (10), 2477-2486 (2005)
   PUBMED   15885097
  REMARK    GeneRIF: These data are consistent with the presence of a triad of
            zinc atoms at the active site of rat intestinal alkaline
            phosphatase isozymes rIAP-I, but not rIAP-II or human placental
            alkaline phosphatase.
REFERENCE   7  (bases 1 to 2226)
  AUTHORS   Xie,Q. and Alpers,D.H.
  TITLE     The two isozymes of rat intestinal alkaline phosphatase are
            products of two distinct genes
  JOURNAL   Physiol Genomics 3 (1), 1-8 (2000)
   PUBMED   11015594
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2226)
  AUTHORS   Engle,M.J., Mahmood,A. and Alpers,D.H.
  TITLE     Two rat intestinal alkaline phosphatase isoforms with different
            carboxyl-terminal peptides are both membrane-bound by a glycan
            phosphatidylinositol linkage
  JOURNAL   J Biol Chem 270 (20), 11935-11940 (1995)
   PUBMED   7744844
REFERENCE   9  (bases 1 to 2226)
  AUTHORS   Engle,M.J. and Alpers,D.H.
  TITLE     The two mRNAs encoding rat intestinal alkaline phosphatase
            represent two distinct nucleotide sequences
  JOURNAL   Clin Chem 38 (12), 2506-2509 (1992)
   PUBMED   1458592
REFERENCE   10 (bases 1 to 2226)
  AUTHORS   Lowe,M., Strauss,A.W., Alpers,R., Seetharam,S. and Alpers,D.H.
  TITLE     Molecular cloning and expression of a cDNA encoding the
            membrane-associated rat intestinal alkaline phosphatase
  JOURNAL   Biochim Biophys Acta 1037 (2), 170-177 (1990)
   PUBMED   2155025
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000009.1.
            
            On Mar 30, 2010 this sequence version replaced NM_022665.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643288.38577.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-115               JAXUCZ010000009.1  95224666-95224780   c
            116-232             JAXUCZ010000009.1  95224464-95224580   c
            233-348             JAXUCZ010000009.1  95224243-95224358   c
            349-523             JAXUCZ010000009.1  95223914-95224088   c
            524-696             JAXUCZ010000009.1  95223673-95223845   c
            697-831             JAXUCZ010000009.1  95223299-95223433   c
            832-904             JAXUCZ010000009.1  95223129-95223201   c
            905-1039            JAXUCZ010000009.1  95222877-95223011   c
            1040-1231           JAXUCZ010000009.1  95222600-95222791   c
            1232-1348           JAXUCZ010000009.1  95222282-95222398   c
            1349-2226           JAXUCZ010000009.1  95221314-95222191   c
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q35"
     gene            1..2226
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /note="alkaline phosphatase, intestinal"
                     /db_xref="GeneID:24197"
                     /db_xref="RGD:2099"
     exon            1..115
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..1668
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /EC_number="3.1.3.1"
                     /note="intestinal alkaline phosphatase 1; intestinal-type
                     alkaline phosphatase 1; IAP-I; IAP-1; Alkaline phosphatase
                     1, intestinal, defined by SSR; intestinal alkaline
                     phosphatase I IAP-I"
                     /codon_start=1
                     /product="intestinal-type alkaline phosphatase 1
                     precursor"
                     /protein_id="NP_073156.3"
                     /db_xref="GeneID:24197"
                     /db_xref="RGD:2099"
                     /translation="MQGDWVLLLLLGLRIHLSFGVIPVEEENPVFWNQKAKEALDVAK
                     KLQPIQTSAKNLILFLGDGMGVPTVTATRILKGQLGGHLGPETPLAMDHFPFTALSKT
                     YNVDRQVPDSAGTATAYLCGVKANYKTIGVSAAARFNQCNSTFGNEVFSVMHRAKKAG
                     KSVGVVTTTRVQHASPAGTYAHTVNRDWYSDADMPSSALQEGCKDIATQLISNMDIDV
                     ILGGGRKFMFPKGTPDPEYPGDSDQSGVRLDSRNLVEEWLAKYQGTRYVWNREQLMQA
                     SQDPAVTRLMGLFEPAEMKYDVNRNASADPSLAEMTEVAVRLLSRNPQGFYLFVEGGR
                     IDQGHHAGTAYLALTEAVMFDSAIEKASQLTNEKDTLTLITADHSHVFAFGGYTLRGT
                     SIFGLAPLNAQDGKSYTSILYGNGPGYVLNSGNRPNVTDAESGDVNYKQQAAVPLSSE
                     THGGEDVAIFARGPQAHLVHGVQEQNYIAHVMAFAGCLEPYTDCGLAPPADENRPTTP
                     VQNSAITMNNVLLSLQLLVSMLLLVGTALVVS"
     exon            116..232
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            233..348
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            349..523
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            524..696
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            697..831
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            832..904
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1039
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1231
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1348
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..2226
                     /gene="Alpi"
                     /gene_synonym="Alp1; S51097"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgataccttg gttccccagc tgggcgtcca tcaatagccc caaccatgca gggagactgg
       61 gtgctgctgc tgcttttggg tctcaggata catctgtcct ttggtgtcat cccagtggag
      121 gaggagaacc cagtcttctg gaatcaaaag gcgaaggagg ccctggatgt tgccaaaaag
      181 ctgcagccca ttcagacatc ggccaagaac ctcatcctct tcctgggaga tgggatgggg
      241 gtacccacag tgacagccac caggatacta aagggacaac tgggtggtca tctaggacct
      301 gagacacccc tggccatgga ccacttccca ttcacggctc tgtccaagac atacaacgtg
      361 gacagacagg tcccagacag tgcaggcaca gccaccgcct atctctgtgg ggtcaaagcc
      421 aactacaaga ccatcggtgt gagcgcggct gctcgattca accagtgcaa cagcacattt
      481 ggcaacgagg tcttctcagt gatgcaccgt gcaaagaaag cagggaagtc tgtgggcgtg
      541 gtgaccacga ccagagtgca gcatgcctct ccagccggca cctacgcgca cacggttaac
      601 cgagactggt actcggatgc agacatgcct tcctccgccc tgcaggaggg ctgcaaggac
      661 atcgccacac agctcatttc caacatggac attgatgtga tccttggtgg aggccgaaag
      721 ttcatgtttc ccaaggggac accagaccct gaatacccag gtgactctga ccagtctgga
      781 gtcaggctgg atagtcggaa cttggtggag gagtggctgg caaaatacca ggggacccgg
      841 tatgtttgga atcgagagca gctcatgcag gcctctcagg acccagcagt gactcgcctc
      901 atgggcctct ttgagccggc agaaatgaaa tatgatgtca accgaaacgc ctcagcagac
      961 ccctccctgg cagagatgac agaggtggcc gtgcgcctgc tgagcaggaa tccacaaggc
     1021 ttctacctct ttgtagaagg aggtcgtatt gaccaaggcc accatgcggg cacggcttac
     1081 ctggcactga ctgaggctgt catgttcgac tccgccatcg aaaaggccag ccagcttacc
     1141 aatgagaagg acacgctaac gctcatcact gcggaccact cccatgtctt tgcttttggt
     1201 ggctatacac ttagggggac ctccatcttt ggtctggccc cactcaatgc ccaggatggc
     1261 aaatcctaca cctccattct ctatggcaac ggcccaggtt atgtgcttaa ttcaggcaac
     1321 cggcccaatg tcactgatgc cgagagtggt gatgtcaact acaagcagca ggcagcagta
     1381 cccctgtcgt cagagaccca cggtggggag gacgtggcca tattcgcgcg tggcccacag
     1441 gcgcacctgg tgcacggagt gcaggaacag aactacatcg cgcacgtcat ggccttcgca
     1501 ggctgcctgg agccctacac cgactgcggt ctggcgcccc ctgcagacga gaacagacca
     1561 accaccccgg tccagaacag tgccatcact atgaacaatg tccttctgtc cctgcagctg
     1621 ctggtcagca tgctgctgct agtcgggaca gccttggtgg tgtcctgatg gtcaggtccc
     1681 ccactaggct cctccctctg ctggttgctc cccacgaccc cccatagatt cctgtgcctt
     1741 gtcagtcctg agttcatcat tctctaccca agggtctcaa cgctggcctt tgcaccccaa
     1801 cttggttgtt ctcttatcca gatgctgaaa cattgtcccg gcccacagtt ctaagggcca
     1861 cctctgggaa tatactctga gatttgcttt ataactctgg gcttccgctt ctcaactgca
     1921 gtgggaggta ccgaacggta tccagaaagc tacagtttgt ggctgtccca gactcccctt
     1981 caatccatca cccttcacag ggaccaagta actcacattt tcaggcctta gcttgttcct
     2041 atgaaaggaa atctcaaaag tccaggagac tgtgggttct caagcaagca gcgcagagca
     2101 gccttgggcc cccacccctg gtccatctca ggctgcatat agaaaccgga caaagaatat
     2161 taccgactct tgtccccagt ccttgaagag acacaatgac aacaaaatta aatgcttata
     2221 taaggt
//
