LOCUS       NM_012822               2540 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus arachidonate 5-lipoxygenase (Alox5), mRNA.
ACCESSION   NM_012822 XM_001054699 XM_006237140
VERSION     NM_012822.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Feng,D.D., Chen,J.H., Chen,Y.F., Cao,Q., Li,B.J., Chen,X.Q., Jin,R.
            and Zhou,G.P.
  TITLE     MALAT1 binds to miR-188-3p to regulate ALOX5 activity in the lung
            inflammatory response of neonatal bronchopulmonary dysplasia
  JOURNAL   Mol Immunol 160, 67-79 (2023)
   PUBMED   37385102
  REMARK    GeneRIF: MALAT1 binds to miR-188-3p to regulate ALOX5 activity in
            the lung inflammatory response of neonatal bronchopulmonary
            dysplasia.
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Xie,S., Chen,M., Fang,W., Liu,S., Wu,Q., Liu,C., Xing,Y., Shi,W.,
            Xu,M., Zhang,M., Chen,S., Zeng,X., Wang,S., Deng,W. and Tang,Q.
  TITLE     Diminished arachidonate 5-lipoxygenase perturbs phase separation
            and transcriptional response of Runx2 to reverse pathological
            ventricular remodeling
  JOURNAL   EBioMedicine 86, 104359 (2022)
   PUBMED   36395739
  REMARK    GeneRIF: Diminished arachidonate 5-lipoxygenase perturbs phase
            separation and transcriptional response of Runx2 to reverse
            pathological ventricular remodeling.
REFERENCE   3  (bases 1 to 2540)
  AUTHORS   Marbach-Breitruck,E., Kutzner,L., Rothe,M., Gurke,R., Schreiber,Y.,
            Reddanna,P., Schebb,N.H., Stehling,S., Wieler,L.H., Heydeck,D. and
            Kuhn,H.
  TITLE     Functional Characterization of Knock-In Mice Expressing a
            12/15-Lipoxygenating Alox5 Mutant Instead of the 5-Lipoxygenating
            Wild-Type Enzyme
  JOURNAL   Antioxid Redox Signal 32 (1), 1-17 (2020)
   PUBMED   31642348
REFERENCE   4  (bases 1 to 2540)
  AUTHORS   Ivanov,I., Golovanov,A.B., Ferretti,C., Canyelles-Nino,M.,
            Heydeck,D., Stehling,S., Lluch,J.M., Gonzalez-Lafont,A. and Kuhn,H.
  TITLE     Mutations of Triad Determinants Changes the Substrate Alignment at
            the Catalytic Center of Human ALOX5
  JOURNAL   ACS Chem Biol 14 (12), 2768-2782 (2019)
   PUBMED   31664810
REFERENCE   5  (bases 1 to 2540)
  AUTHORS   Tian,W., Jiang,X., Sung,Y.K., Shuffle,E., Wu,T.H., Kao,P.N.,
            Tu,A.B., Dorfmuller,P., Cao,A., Wang,L., Peng,G., Kim,Y., Zhang,P.,
            Chappell,J., Pasupneti,S., Dahms,P., Maguire,P., Chaib,H.,
            Zamanian,R., Peters-Golden,M., Snyder,M.P., Voelkel,N.F.,
            Humbert,M., Rabinovitch,M. and Nicolls,M.R.
  TITLE     Phenotypically Silent Bone Morphogenetic Protein Receptor 2
            Mutations Predispose Rats to Inflammation-Induced Pulmonary
            Arterial Hypertension by Enhancing the Risk for Neointimal
            Transformation
  JOURNAL   Circulation 140 (17), 1409-1425 (2019)
   PUBMED   31462075
REFERENCE   6  (bases 1 to 2540)
  AUTHORS   Brock,T.G., McNish,R.W. and Peters-Golden,M.
  TITLE     Translocation and leukotriene synthetic capacity of nuclear
            5-lipoxygenase in rat basophilic leukemia cells and alveolar
            macrophages
  JOURNAL   J Biol Chem 270 (37), 21652-21658 (1995)
   PUBMED   7665580
REFERENCE   7  (bases 1 to 2540)
  AUTHORS   Goulet,J.L., Snouwaert,J.N., Latour,A.M., Coffman,T.M. and
            Koller,B.H.
  TITLE     Altered inflammatory responses in leukotriene-deficient mice
  JOURNAL   Proc Natl Acad Sci U S A 91 (26), 12852-12856 (1994)
   PUBMED   7809134
REFERENCE   8  (bases 1 to 2540)
  AUTHORS   Smith,S.R., Watnick,A.W., Bryant,R.W., Billah,M. and Siegel,M.I.
  TITLE     Actions of a 5-lipoxygenase inhibitor, Sch 40120, on acute
            inflammatory responses
  JOURNAL   J Pharmacol Exp Ther 262 (2), 721-728 (1992)
   PUBMED   1386887
REFERENCE   9  (bases 1 to 2540)
  AUTHORS   Gross,N.J., Holloway,N.O. and Narine,K.R.
  TITLE     Effects of some nonsteroidal anti-inflammatory agents on
            experimental radiation pneumonitis
  JOURNAL   Radiat Res 127 (3), 317-324 (1991)
   PUBMED   1886988
REFERENCE   10 (bases 1 to 2540)
  AUTHORS   Balcarek,J.M., Theisen,T.W., Cook,M.N., Varrichio,A., Hwang,S.M.,
            Strohsacker,M.W. and Crooke,S.T.
  TITLE     Isolation and characterization of a cDNA clone encoding rat
            5-lipoxygenase
  JOURNAL   J Biol Chem 263 (27), 13937-13941 (1988)
   PUBMED   3417684
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On or before Dec 1, 2020 this sequence version replaced
            XM_006237140.2, NM_012822.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.24872.1,
                                           SRR26643292.19396.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-240               JAXUCZ010000004.1  151250887-151251126 c
            241-439             JAXUCZ010000004.1  151242840-151243038 c
            440-521             JAXUCZ010000004.1  151237885-151237966 c
            522-644             JAXUCZ010000004.1  151222306-151222428 c
            645-751             JAXUCZ010000004.1  151218324-151218430 c
            752-924             JAXUCZ010000004.1  151217315-151217487 c
            925-1071            JAXUCZ010000004.1  151214301-151214447 c
            1072-1275           JAXUCZ010000004.1  151209133-151209336 c
            1276-1362           JAXUCZ010000004.1  151208168-151208254 c
            1363-1541           JAXUCZ010000004.1  151207390-151207568 c
            1542-1663           JAXUCZ010000004.1  151207082-151207203 c
            1664-1764           JAXUCZ010000004.1  151206737-151206837 c
            1765-1935           JAXUCZ010000004.1  151206073-151206243 c
            1936-2540           JAXUCZ010000004.1  151203949-151204553 c
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     gene            1..2540
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /note="arachidonate 5-lipoxygenase"
                     /db_xref="GeneID:25290"
                     /db_xref="RGD:2096"
     exon            1..240
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..2115
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /EC_number="1.13.11.34"
                     /note="5-LO; 5 - Lipoxygenase; 5-lipoxygenase"
                     /codon_start=1
                     /product="polyunsaturated fatty acid 5-lipoxygenase"
                     /protein_id="NP_036954.2"
                     /db_xref="GeneID:25290"
                     /db_xref="RGD:2096"
                     /translation="MPSYTVTVATGSQWFAGTDDYIYLSLIGSAGCSEKHLLDKAFYN
                     DFERGAVDSYDVTVDEELGEIYLVKIEKRKYWLHDDWYLKYITLKTPHGDYIEFPCYR
                     WITGEGEIVLRDGCAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKC
                     HKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWHDFADFEKIFVKISNT
                     ISERVKNHWQEDLMFGYQFLNGCNPVLIKRCTELPKKLPVTTEMVECSLERQLSLEQE
                     VQEGNIFIVDYELLDGIDANKTDPCTHQFLAAPICLLYKNLANKIVPIAIQLNQTPGE
                     KNPIFLPTDSKYDWLLAKIWVRSSDFHIHQTITHLLRTHLVSEVFGIAMYRQLPAVHP
                     LFKLLVAHVRFTIAINTKAREQLICEYGLFDKANATGGGGHVQMVQRAVQDLTYSSLC
                     FPEAIKARGMDNTEDIPYYFYRDDGLLVWEAIQSFTTEVVSIYYEDDQVVEEDQELQD
                     FVKDVYVYGMRGRKASGFPKSIKSREKLSEYLTVVIFTASAQHAAVNFGQYDWCSWIP
                     NAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEE
                     HFIEKPVKEAMIRFRKNLEAIVSVIAERNKNKKLPYYYLSPDRIPNSVAI"
     exon            241..439
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            440..521
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            522..644
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            645..751
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            752..924
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1071
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1275
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1362
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1541
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1663
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1764
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1935
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2540
                     /gene="Alox5"
                     /gene_synonym="LOX5A"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttgagtgac agagtcaaga atctggtggg ctgccacctg aacttcccgg gctcctgcgc
       61 ccacgccagc agcgctcact tcccagagcc atgccttcct acactgtcac cgtagccacc
      121 ggtagccagt ggttcgcggg caccgacgac tacatttacc tcagcctcat tggctctgcg
      181 ggctgcagtg agaagcatct tcttgacaaa gctttctaca atgacttcga gcgcggcgcg
      241 gtggactcct atgacgtcac tgtggatgaa gaactgggtg agatctacct agtcaaaatc
      301 gagaagcgca aatactggct ccatgatgac tggtacttga aatacatcac actgaagaca
      361 ccccacgggg actacataga gttcccttgt tatcgttgga tcacaggcga gggcgagatt
      421 gtcctgaggg atggatgtgc aaaattggcc cgagatgacc aaatccacat cctcaagcag
      481 cacaggcgga aagaactgga aacacgtcag aaacagtatc gatggatgga gtggaacccc
      541 ggcttccctt tgagtattga tgctaaatgc cacaaggatc tgccccgaga tatccagttt
      601 gatagtgaaa agggagtgga ctttgttctg aactactcaa aagcgatgga gaacctgttc
      661 atcaatcgct tcatgcacat gttccagtct tcctggcatg actttgctga ctttgagaaa
      721 atcttcgtca aaatcagcaa cactatttct gagagagtca agaaccactg gcaagaggac
      781 ctcatgtttg gctaccagtt cctgaatggc tgcaacccag tactcatcaa gcgctgcaca
      841 gagttgccta agaagctccc agtgaccaca gaaatggtgg agtgcagcct agagcggcag
      901 ctcagtttag aacaggaagt acaggaaggg aacattttca tcgttgatta cgaactactg
      961 gatggcattg atgctaacaa aactgacccc tgtacacacc agttcctggc cgcccccatc
     1021 tgcctgctgt ataagaacct agccaacaag attgttccca tcgccatcca gctcaaccaa
     1081 acccctggag agaagaaccc aattttcctc cctacggact caaaatacga ctggcttttg
     1141 gccaaaatct gggtgcgttc aagtgacttc catatccatc aaacaatcac ccatcttctc
     1201 cgcacacatc tggtgtctga ggtgttcggt attgccatgt accgccagct gcctgctgtg
     1261 cacccccttt tcaagctgct ggtagcccat gtgaggttca ccattgccat caacactaag
     1321 gcccgggaac agcttatctg tgagtacggc ctttttgaca aggccaatgc caccggaggt
     1381 ggtgggcacg tgcagatggt gcagagagct gtccaggacc tgacctattc ctccctgtgc
     1441 ttcccggagg ccatcaaggc ccggggcatg gacaacaccg aggacatccc ctactacttc
     1501 tatcgtgatg atggactgct cgtgtgggaa gctatccagt cgttcacaac tgaggtggta
     1561 agcatctact atgaggatga ccaggtggtg gaggaggacc aggaactgca ggacttcgtg
     1621 aaggatgttt acgtttatgg catgcggggc agaaaggcct caggtttccc caagtccatc
     1681 aagagcaggg agaaattgtc tgagtacctg acggtggtga tcttcacagc ctcggcccag
     1741 catgcagctg taaactttgg ccagtatgac tggtgctcct ggatccccaa cgctcctcca
     1801 actatgcggg ccccaccacc cacggccaag ggtgtggtca ccatcgagca gattgtggat
     1861 actctaccag accgtggccg ctcatgttgg catctaggtg cagtgtgggc cttgagccag
     1921 tttcaagaga atgagctgtt tctgggcatg tacccagagg agcatttcat cgagaagcca
     1981 gtgaaagaag ccatgattcg attccgcaag aacctggagg ccatcgtcag cgtgattgcc
     2041 gagcgcaata agaacaaaaa gctcccctac tactacctgt caccagacag gattccaaac
     2101 agtgtagcca tctaaggctt tgccgtccct gtcccagcag ctctctgggc aggccagtgg
     2161 cttgcctggc aggctgtaga tcttccagca gctggtgcct ctcccaagct agcagtgccg
     2221 ctcttgggcc caggtgtggt tgtaaactaa aggctgttct aggtggggaa atttcacaga
     2281 gcttcagcac atgctacttc ttcctgtctt cagtgacata gttcaggggc ctcccatcca
     2341 gagcaggggt gtgcggctgt gtcccccagt ccagcttagt aacctcttca ctcagtagca
     2401 acagagtaag tgataatgtt ccactgggtc aggggtccat cattctactt atgcttcctc
     2461 caactgcctg catacagtag gtgcttagag aaacttctta gattaagagt ttgttataaa
     2521 ataaagttca tttaaaacag
//
