LOCUS       NM_001039377            2268 bp    mRNA    linear   ROD 06-DEC-2024
DEFINITION  Rattus norvegicus arachidonate 12-lipoxygenase, 12R type (Alox12b),
            mRNA.
ACCESSION   NM_001039377 XM_220598
VERSION     NM_001039377.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Gregus,A.M., Dumlao,D.S., Wei,S.C., Norris,P.C., Catella,L.C.,
            Meyerstein,F.G., Buczynski,M.W., Steinauer,J.J., Fitzsimmons,B.L.,
            Yaksh,T.L. and Dennis,E.A.
  TITLE     Systematic analysis of rat 12/15-lipoxygenase enzymes reveals
            critical role for spinal eLOX3 hepoxilin synthase activity in
            inflammatory hyperalgesia
  JOURNAL   FASEB J 27 (5), 1939-1949 (2013)
   PUBMED   23382512
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Garcia-Verdugo,I., BenMohamed,F., Tattermusch,S., Leduc,D.,
            Charpigny,G., Chignard,M., Ollero,M. and Touqui,L.
  TITLE     A role for 12R-lipoxygenase in MUC5AC expression by respiratory
            epithelial cells
  JOURNAL   Eur Respir J 40 (3), 714-723 (2012)
   PUBMED   22441738
REFERENCE   3  (bases 1 to 2268)
  AUTHORS   Zheng,Y., Yin,H., Boeglin,W.E., Elias,P.M., Crumrine,D., Beier,D.R.
            and Brash,A.R.
  TITLE     Lipoxygenases mediate the effect of essential fatty acid in skin
            barrier formation: a proposed role in releasing
            omega-hydroxyceramide for construction of the corneocyte lipid
            envelope
  JOURNAL   J Biol Chem 286 (27), 24046-24056 (2011)
   PUBMED   21558561
REFERENCE   4  (bases 1 to 2268)
  AUTHORS   Liska,F., Gosele,C., Rivkin,E., Tres,L., Cardoso,M.C., Domaing,P.,
            Krejci,E., Snajdr,P., Lee-Kirsch,M.A., de Rooij,D.G., Kren,V.,
            Krenova,D., Kierszenbaum,A.L. and Hubner,N.
  TITLE     Rat hd mutation reveals an essential role of centrobin in spermatid
            head shaping and assembly of the head-tail coupling apparatus
  JOURNAL   Biol Reprod 81 (6), 1196-1205 (2009)
   PUBMED   19710508
  REMARK    Erratum:[Biol Reprod. 2010 Apr;82(4):804. Rooij, Dirk G de
            [corrected to de Rooij, Dirk G]]
REFERENCE   5  (bases 1 to 2268)
  AUTHORS   Li,J., Wang,H. and Rosenberg,P.A.
  TITLE     Vitamin K prevents oxidative cell death by inhibiting activation of
            12-lipoxygenase in developing oligodendrocytes
  JOURNAL   J Neurosci Res 87 (9), 1997-2005 (2009)
   PUBMED   19235890
  REMARK    GeneRIF: In summary, our data indicate that vitamin K prevents
            oxidative cell death by blocking activation of 12-LOX and ROS
            generation.
REFERENCE   6  (bases 1 to 2268)
  AUTHORS   Liska,F., Gosele,C., Kren,V., Hubner,N. and Krenova,D.
  TITLE     Molecular analysis of the sex hormone-binding globulin gene in the
            rat hypodactylous mutation (Hd)
  JOURNAL   Folia Biol (Praha) 50 (2), 63-68 (2004)
   PUBMED   15222128
REFERENCE   7  (bases 1 to 2268)
  AUTHORS   Yoshimoto,T. and Takahashi,Y.
  TITLE     Arachidonate 12-lipoxygenases
  JOURNAL   Prostaglandins Other Lipid Mediat 68-69, 245-262 (2002)
   PUBMED   12432922
  REMARK    Review article
REFERENCE   8  (bases 1 to 2268)
  AUTHORS   Siebert,M., Krieg,P., Lehmann,W.D., Marks,F. and Furstenberger,G.
  TITLE     Enzymic characterization of epidermis-derived 12-lipoxygenase
            isoenzymes
  JOURNAL   Biochem J 355 (Pt 1), 97-104 (2001)
   PUBMED   11256953
REFERENCE   9  (bases 1 to 2268)
  AUTHORS   Krieg,P., Siebert,M., Kinzig,A., Bettenhausen,R., Marks,F. and
            Furstenberger,G.
  TITLE     Murine 12(R)-lipoxygenase: functional expression, genomic structure
            and chromosomal localization
  JOURNAL   FEBS Lett 446 (1), 142-148 (1999)
   PUBMED   10100631
REFERENCE   10 (bases 1 to 2268)
  AUTHORS   Sun,D., McDonnell,M., Chen,X.S., Lakkis,M.M., Li,H., Isaacs,S.N.,
            Elsea,S.H., Patel,P.I. and Funk,C.D.
  TITLE     Human 12(R)-lipoxygenase and the mouse ortholog. Molecular cloning,
            expression, and gene chromosomal assignment
  JOURNAL   J Biol Chem 273 (50), 33540-33547 (1998)
   PUBMED   9837935
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY903231.1.
            
            On Feb 4, 2006 this sequence version replaced XM_220598.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY903231.1, AY903232.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="WHD"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q24"
     gene            1..2268
                     /gene="Alox12b"
                     /note="arachidonate 12-lipoxygenase, 12R type"
                     /db_xref="GeneID:287425"
                     /db_xref="RGD:1305330"
     exon            1..288
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..2247
                     /gene="Alox12b"
                     /EC_number="1.13.11.-"
                     /note="12R-LOX; 12R-lipoxygenase; epidermis-type
                     lipoxygenase 12"
                     /codon_start=1
                     /product="arachidonate 12-lipoxygenase, 12R-type"
                     /protein_id="NP_001034466.1"
                     /db_xref="GeneID:287425"
                     /db_xref="RGD:1305330"
                     /translation="MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRD
                     FATGAVDDYTVQCQQDLGELIIIRLHKEPHSFLPKDPWYCNYVQICAPNCRVYHFPAY
                     QWMDGYETLSLREATGKTTADDTLPILLEHRQEEIRAKKDFYHWRVFVPGLPNYVDIP
                     SYHPPPRRCRNPNRPEWNGYIPGFPILINIKATRFLNLNLRFSFVKTASFFYRLGPMA
                     LAFKLRGLVDRKRSWKRLKDIKNIFPATKTVVSEYVAEHWTEDSFFGYQYLNGINPGH
                     IRRCMQIPDKFPVTDEMVAPFLGEGTCLQAELEKGNIYLADYRILDGIPTVELNGQKQ
                     HHCAPICLLHFGPDGNMMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYS
                     HEAVAHLLESHLIGEAFCLALLRNLPMCHPLYKLLIPHTRYNVQINSIGRALLLNKGG
                     LSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGVQDLPGYYFRDDSLAVWYA
                     MERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLERESSGFPTCLRTVPELIEY
                     VTMVMYTCSARHAAVNTGQLEYTSWMPNFPSSMRNPPMQSKGLTTLQTFMDTLPDVKT
                     TCIVLLVLWTLCREPDDRRPLGHFPDIHFVEEAPRRSMEAFRQNLNQISHNIRQRNKC
                     LNLPYYYLDPVLIENSISI"
     exon            289..493
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            494..575
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            576..668
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            669..791
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            792..895
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1068
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..1212
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1416
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1503
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1673
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1795
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1896
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..2067
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
     exon            2068..2268
                     /gene="Alox12b"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgctccatca ctgactgctc catcgctgac tgcttcatca ctcttcgctg tctggcttcc
       61 attccctaac tcgtttctaa ctgcgtggcc ctaaccatag tgtccgggat tgagccctct
      121 cttgcctcct gaagagcggc catggccacc tacaaagtca aggtggccac gggcactgac
      181 ttcttttcgg ggacgctgga ctcaatttcc ctgaccatcg tgggcaccca aggagagagc
      241 cataagcagc ggttgaacca ctttgggaga gactttgcca caggggccgt ggatgactac
      301 acagtgcagt gccagcagga cctgggggag ctcatcatca tccgtctgca caaggagcct
      361 cattccttct tacccaagga cccctggtac tgcaactatg tgcagatctg cgctcccaac
      421 tgtcgtgtct accacttccc agcttaccag tggatggatg gctatgagac cctgtcactc
      481 cgggaggcca caggaaagac aacagcagat gacacacttc ccatcctttt ggaacacaga
      541 caagaagaga tcagagccaa gaaagacttc taccactgga gggtctttgt tcccggcctt
      601 cccaactatg tggacatccc cagttaccat cctcctccca gacggtgccg caaccccaac
      661 cggcctgagt ggaatggtta tattccagga ttcccaattc tcatcaacat taaggccacc
      721 aggttcttaa acttaaatct tcgtttctcc ttcgtcaaga cagcctcctt cttctaccgc
      781 ctagggccca tggcactggc cttcaagctt cgtggcctgg tggatcgcaa acgctcttgg
      841 aagagactaa aggacattaa gaacattttc cctgctacca agactgtcgt ctctgagtat
      901 gtggccgagc actggactga agatagcttc tttgggtacc agtacctcaa cggcatcaac
      961 ccaggccata tccgaagatg catgcagatc ccagacaagt tcccagttac agatgaaatg
     1021 gtggccccat tcctgggtga aggaacatgc ctgcaggcag agctggagaa ggggaatatc
     1081 tacctggcag actaccgcat cttggatggc atccccacgg tggagctcaa tggtcagaag
     1141 cagcatcact gtgccccgat ctgcttgctg cactttggtc ccgatggtaa catgatgcct
     1201 attgccattc agctcagcca gactcctggg cctgattgtc ccatcttcct gcctaatgat
     1261 tctgagtggg actggctgtt ggctaaaaca tgggtacgct acgctgagtt ctacagccac
     1321 gaggccgttg cccacttact ggagagtcac ctcatcgggg aagctttctg cttggccctc
     1381 ctgaggaacc tgcccatgtg ccaccccctg tataagctcc tcattcctca cactcgctac
     1441 aacgtccaga tcaacagcat tgggcgggcc ctccttctca acaaaggggg tctctctgcc
     1501 agggccatgt ccctgggcct agagggcttc gcacaggtga tggttcgggg tctgtctgag
     1561 ctcacctaca aaagcctctg catccccaat gactttgtgg agcgcggggt tcaggatctg
     1621 cctgggtatt atttccgtga cgacagcttg gcggtgtggt atgccatgga gaggtacgtg
     1681 acagagatca tcacttatta ttacccgaat gacgcagctg tggagggcga cccggaattg
     1741 cagtgctggg tgcaggagat attcaaggag tgtctcctgg agcgtgagag ctcaggtttt
     1801 cccacctgct tgcgaactgt acctgagctg atcgaatacg tcacgatggt catgtacacc
     1861 tgctcagcga ggcatgcagc tgtcaacaca ggccagttgg aatacacttc ttggatgccc
     1921 aacttcccgt catccatgag gaacccgccg atgcagtcca aggggctgac tactttgcag
     1981 acattcatgg acactttgcc agacgtgaag accacatgca ttgtgctctt ggtgctctgg
     2041 actctctgcc gggagcctga tgacaggcgg ccacttggcc acttcccgga catccatttt
     2101 gtggaggagg cccctcggag gagtatggag gcttttcgtc agaacctcaa ccagatctct
     2161 cacaacatcc gccagcgcaa taagtgcctc aacctcccgt attactacct ggacccggtg
     2221 ctgattgaga acagtatttc catctagtct acccttctgc cttccctc
//
