LOCUS       NM_001106604            9965 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus ALMS1, centrosome and basal body associated
            protein (Alms1), mRNA.
ACCESSION   NM_001106604 XM_001074073 XM_216189
VERSION     NM_001106604.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Jaykumar,A.B., Caceres,P.S., King-Medina,K.N., Liao,T.D., Datta,I.,
            Maskey,D., Naggert,J.K., Mendez,M., Beierwaltes,W.H. and Ortiz,P.A.
  TITLE     Role of Alstrom syndrome 1 in the regulation of blood pressure and
            renal function
  JOURNAL   JCI Insight 3 (21), 95076 (2018)
   PUBMED   30385718
  REMARK    GeneRIF: In this study, we show that gene deletion of ALMS1
            decreases NKCC2 endocytosis establishing a link between the genetic
            susceptibility to hypertension, CKD, and the expression of ALMS1
            through its role in a salt-reabsorbing tubular segment of the
            kidney. These data point to ALMS1 as a potentially novel gene
            involved in BP and renal function regulation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   Poekes,L., Legry,V., Schakman,O., Detrembleur,C., Bol,A.,
            Horsmans,Y., Farrell,G.C. and Leclercq,I.A.
  TITLE     Defective adaptive thermogenesis contributes to metabolic syndrome
            and liver steatosis in obese mice
  JOURNAL   Clin Sci (Lond) 131 (4), 285-296 (2017)
   PUBMED   27803297
REFERENCE   3  (bases 1 to 9965)
  AUTHORS   Liang,X., Li,H., Li,H., Xu,F., Dong,F. and Sui,R.
  TITLE     Novel ALMS1 mutations in Chinese patients with Alstrom syndrome
  JOURNAL   Mol Vis 19, 1885-1891 (2013)
   PUBMED   24049434
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9965)
  AUTHORS   Pineiro-Gallego,T., Corton,M., Ayuso,C., Baiget,M. and Valverde,D.
  TITLE     Molecular approach in the study of Alstrom syndrome: analysis of
            ten Spanish families
  JOURNAL   Mol Vis 18, 1794-1802 (2012)
   PUBMED   22876109
REFERENCE   5  (bases 1 to 9965)
  AUTHORS   Collin,G.B., Marshall,J.D., King,B.L., Milan,G., Maffei,P.,
            Jagger,D.J. and Naggert,J.K.
  TITLE     The Alstrom syndrome protein, ALMS1, interacts with alpha-actinin
            and components of the endosome recycling pathway
  JOURNAL   PLoS One 7 (5), e37925 (2012)
   PUBMED   22693585
REFERENCE   6  (bases 1 to 9965)
  AUTHORS   Arsov,T., Silva,D.G., O'Bryan,M.K., Sainsbury,A., Lee,N.J.,
            Kennedy,C., Manji,S.S., Nelms,K., Liu,C., Vinuesa,C.G., de
            Kretser,D.M., Goodnow,C.C. and Petrovsky,N.
  TITLE     Fat aussie--a new Alstrom syndrome mouse showing a critical role
            for ALMS1 in obesity, diabetes, and spermatogenesis
  JOURNAL   Mol Endocrinol 20 (7), 1610-1622 (2006)
   PUBMED   16513793
REFERENCE   7  (bases 1 to 9965)
  AUTHORS   Patel,S., Minton,J.A., Weedon,M.N., Frayling,T.M., Ricketts,C.,
            Hitman,G.A., McCarthy,M.I., Hattersley,A.T., Walker,M. and
            Barrett,T.G.
  TITLE     Common variations in the ALMS1 gene do not contribute to
            susceptibility to type 2 diabetes in a large white UK population
  JOURNAL   Diabetologia 49 (6), 1209-1213 (2006)
   PUBMED   16601972
REFERENCE   8  (bases 1 to 9965)
  AUTHORS   Collin,G.B., Cyr,E., Bronson,R., Marshall,J.D., Gifford,E.J.,
            Hicks,W., Murray,S.A., Zheng,Q.Y., Smith,R.S., Nishina,P.M. and
            Naggert,J.K.
  TITLE     Alms1-disrupted mice recapitulate human Alstrom syndrome
  JOURNAL   Hum Mol Genet 14 (16), 2323-2333 (2005)
   PUBMED   16000322
REFERENCE   9  (bases 1 to 9965)
  AUTHORS   Hearn,T., Spalluto,C., Phillips,V.J., Renforth,G.L., Copin,N.,
            Hanley,N.A. and Wilson,D.I.
  TITLE     Subcellular localization of ALMS1 supports involvement of
            centrosome and basal body dysfunction in the pathogenesis of
            obesity, insulin resistance, and type 2 diabetes
  JOURNAL   Diabetes 54 (5), 1581-1587 (2005)
   PUBMED   15855349
REFERENCE   10 (bases 1 to 9965)
  AUTHORS   Collin,G.B., Marshall,J.D., Ikeda,A., So,W.V., Russell-Eggitt,I.,
            Maffei,P., Beck,S., Boerkoel,C.F., Sicolo,N., Martin,M.,
            Nishina,P.M. and Naggert,J.K.
  TITLE     Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory
            degeneration in Alstrom syndrome
  JOURNAL   Nat Genet 31 (1), 74-78 (2002)
   PUBMED   11941369
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Jul 7, 2010 this sequence version replaced NM_001106604.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760383,
                              SAMEA5760389 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-481               JAXUCZ010000004.1  119683085-119683565
            482-607             JAXUCZ010000004.1  119694633-119694758
            608-800             JAXUCZ010000004.1  119695966-119696158
            801-918             JAXUCZ010000004.1  119701743-119701860
            919-1058            JAXUCZ010000004.1  119702883-119703022
            1059-1177           JAXUCZ010000004.1  119704734-119704852
            1178-1337           JAXUCZ010000004.1  119709275-119709434
            1338-5120           JAXUCZ010000004.1  119713932-119717714
            5121-5254           JAXUCZ010000004.1  119718860-119718993
            5255-7038           JAXUCZ010000004.1  119722173-119723956
            7039-7268           JAXUCZ010000004.1  119734225-119734454
            7269-7394           JAXUCZ010000004.1  119736628-119736753
            7395-7550           JAXUCZ010000004.1  119742763-119742918
            7551-7685           JAXUCZ010000004.1  119747723-119747857
            7686-7775           JAXUCZ010000004.1  119754494-119754583
            7776-8926           JAXUCZ010000004.1  119757332-119758482
            8927-9044           JAXUCZ010000004.1  119775716-119775833
            9045-9248           JAXUCZ010000004.1  119776920-119777123
            9249-9490           JAXUCZ010000004.1  119777413-119777654
            9491-9644           JAXUCZ010000004.1  119778162-119778315
            9645-9708           JAXUCZ010000004.1  119778962-119779025
            9709-9808           JAXUCZ010000004.1  119782507-119782606
            9809-9965           JAXUCZ010000004.1  119783315-119783471
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..9965
                     /gene="Alms1"
                     /note="ALMS1, centrosome and basal body associated
                     protein"
                     /db_xref="GeneID:297408"
                     /db_xref="RGD:1311590"
     exon            1..481
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..9853
                     /gene="Alms1"
                     /note="Alstrom syndrome 1 homolog; Alstrom syndrome
                     protein 1"
                     /codon_start=1
                     /product="centrosome-associated protein ALMS1"
                     /protein_id="NP_001100074.2"
                     /db_xref="GeneID:297408"
                     /db_xref="RGD:1311590"
                     /translation="MEPEDLPWPDELEEEEEEEEEEGKEEAENASAAATEEARTSEET
                     GRLEEEELEGAGPEVDLDFNSESQLQESTDEEEDELAKAWLQAHPDRPGQGFSLTPPP
                     TPPPSSRHQYRPVEHWGKREVIPLTYRAWQQTSYQDNARAQFSNSSTLLLEPEFQWDP
                     EEVQRTESWHHLPQERDSFQTLNISQTGIDRVEGAEVKDLPSQEGGESVQAQCPVKEP
                     KLDLLCSPLLVIQDDFAAPDLPLLTCMTQDQEFEPDSLFQQSELEFAPLRGIPDKSED
                     IEWLARPSEVSEALIQATSESSSDLVNSCFSISQHPLTEGLQGKAKSDVLILFGDAKY
                     ASIYENAGAHSERITELQREVDCSNLSITQAFPSSLPSFVPQEPTIESEYHSSNLRML
                     RVSPDTLLTTLTQTTGSVDQKIGAAAISSPYSHGVQPGSFPQEELPDRHLTEEIRKVS
                     PAPRPAGQKPEMPTVPSSSYSKGIKSVFYRYPMSYSPQGKEPPNVSSVSGSAENALPL
                     TVTSVSQPQRETAKAFYQQTLSDSLLTAETLPVSVIPESGDQKTHLLSGFSLSYSHRG
                     EDLPEDVVKVSTDSGSVDKKADIPTVSSSTYQHKTKPGIIYYQEFPDSHVPIGTRKVV
                     SQSGAAGQKTDLSAVPSTSSSQAEKPVPPSLLTLLGSHLPEDVFKASYVCKSSEEPSG
                     ITALTSVSYSYKGRPNISHQEKFPDSYLIEEAQKILGSTVTVDQKTVTAKEKPSVFYQ
                     QALPEGGLSAEDLQVDQNIAIPMVTSASFSQREKPQVFYQEALSGGHLPGEPPGPPDQ
                     NTEAPTISPSSYFPGEESIIFYQAGFPGNTPSVVPFKVSRISELAEQKTNITTGPSGS
                     YSVGEKSIIFYHQALPDGHLSKEASPAPADLKTGEPPVYSASCSVEVKPIIFYQQPLS
                     DSPLTKGHNVSVVPGPTDQKTGIPAVPSTSQSYIERRSVSYQQEFPNVSEKALKVLGG
                     VRSTEQKTEIPVVSSALLHTEVPTISSQEDLPNLTEEALQILGVAEEVPSVPSRDKTR
                     LISYQKKLPDGTEVSLKSVGFGSTDQKTGAHIVSSSRREKSSGFHQQELPNTCGDALG
                     TFHPEPVAQKVGKPTEPSNIQGKASALSPQQLPASHLTGEALKVSDGSACVAQMPEAP
                     AGPSIFYFHKERFSNYQKASPDCDLTENSLKSSTVPGLSDQKKRPPVSSGSCSHKEKH
                     KISAADLLDAQAAELPTVIQRSCLHREKLAISTVIKPDDPKIPLPTTCHASCAQKVKP
                     VIFVQKQLSGRDQSEDIPKISTVSEPTVANTGAPVLLSSSYPYREKSDFSSPKELPDR
                     HPAKVDMQASSSLGQADQISRLPTVPPGTYSHSEKYQLISEHVQELIDNLNSSESCLN
                     ADIMMPSNSQIDDGVIICKPESSRFANVGCEEIQDIDHGSKTLKEIQTLLMEAENMAL
                     KRCNFSVPLVPFRDVSDVSFVQSKKVVCFKETPTSDVCIQKDSFAEEVPHTKYVQKDI
                     GTQTNLKYQRNVRNWEFISSATFRSPLHEANGTARVAFDENFRQYRATRSVMRSEPED
                     CSTGIGDKIIIPMMTIIKSDSSSDVSDGCCSWDNNLPESLESVSDVFLNFFPYTSPKT
                     SITDSREEEGLSESEDGYGSADSLAAHVKYLLQCESSLNQAKQILKNAEEEEYRVRAQ
                     AWNLKFNLGRDYGYPVSELNEDDRRKVEEIKAKLFGHGRTTHVSEVLQSPRGIGCLPE
                     AVCGHIIIESHEKGCYRTLTAEQPRPDSCHCVFRSVDPSDLIRGHRSPSSWRGRHINL
                     SRSIDQSNPHFKIGNSFQLQSHPPFEKLVPDDIKISKGVGMPFHEHMDPQPSELIEPT
                     CVPAKEMDFSSSSEILPSEPKKQFTTSITFSSHQHSKCISDSSGFKVDVTAGSQCTGP
                     SLGVFKPHIPEEQISPRDLKQKISSPSSLQRHSYTPVTILVDSSRQRQKLPVDFEHSN
                     QKGKLLQRSAFNFSHSEPSVSTNVSFKGVQFSGNDTIVRQDKLISTVEVKEKNVTLTP
                     DLPSCIFLEQGEESHTPHTDLHMRKCPSPSGLDIASCIFLEQHKFFEQSQGQHVDDHE
                     MREDYSLFPKCQGYIAADPSDFPDQQQCKPPGVDGHVRKQHYPLSPVQDCEVEEVQCI
                     PQSYFSNMVNVEAKVSDMVSPDAPDHFTAASTPPSSRKALSCVRITLCPKTPSKLDSG
                     TLDERFHSLDPASKTRINSEFNSDLRIISSRSLEPTSKLLTCKPVAQDQESLVFLGPK
                     SPLDLQVAESSLPDSKTISQDLKTTPPQNSQIVTSRQTQVNISDLEEYSKPEGTPVPA
                     DGSQEQNKAPFTTSSGMLSSDAVTQVTTESPEKMTFSSEIFINADDRGQDIPYPTTQN
                     PSRFASSSSGQQITASQGKDAQPVLLPYKPSGSSKMYYVPLLKPVPSYLDSKSDTTVE
                     SSHSGSNDAIAPDFPPQVLGTRDDDLSTTVNIKHKEGIYSKRAASKGKQPSQKGNAEA
                     PVQMPVTWDKNVYKENQDEIISRRVVIETAGPEEMPSQEKDLAGSSDITVQDRKTGNL
                     PDAKSIKQKEGSLEIEPECHSEFANTTHSVFRSASFYFHHPVHLPQEQDLCRESSGRN
                     VLTQHPWKDLFHCHSGHSGLPPPGPRSDNTEMECTRVKSLSMDLNLGGHEKIHTIKNQ
                     ARDTKEDKRPANDPKKDQKVTPELTSQCPMSLNELWNKYQERQKQQKPSGACDNKELS
                     LVERLDRLAKLLQNPITHSLQASESTQDDRRWGRGARGWTGRRQHQQKGKQQRKWSKS
                     LERGHSTGDFRKSKVLSPHEGGKSSQRNIEKIKLDKYILRKQPDFNNVSNTSLDSRPS
                     EESELLLTDSPNLFSSTESPVESDVLTHTGRDMPLNERSSSISTVDTARLIQAFGHDR
                     LYLSPRQIKLYSSVTNQRRKHLEKPCKHKRKALNTACPQMTSEHTRRRHVQVANRIMS
                     SDSVSSAGSCLSLDSTLSNEENVHMLNKGVQAGNLEIVTGAKKYTQDVGVTFPTPSSS
                     EARLEEDSDMTSWSEEKAKEKKFLSNYLRGKNLRKNKPNPCEGVSWFVPVENRKSESK
                     KENLPKIYRPAISWFEPVTQTKPWREPLREQNWQAQCMNSRGSLGGPGRDSGQSFLQP
                     FVRATLQESLQLHRPDFISRSGERIKRLKLLVQERKLQNLFQNEREALFNTARPLPRR
                     VLLAVQKNKPIGKTEMIRRTRRFYEQLPEVKKKREEEKRKSEYTSYRLRAQHYKMKVT
                     NHLLGRKVPWD"
     exon            482..607
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            608..800
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            801..918
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1058
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1177
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1337
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..5120
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            5121..5254
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            5255..7038
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            7039..7268
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            7269..7394
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            7395..7550
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            7551..7685
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            7686..7775
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            7776..8926
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            8927..9044
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            9045..9248
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            9249..9490
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            9491..9644
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            9645..9708
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            9709..9808
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
     exon            9809..9965
                     /gene="Alms1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagagggcag gtgcgcctgc gcacagccgc aaccgccagt caactcgctt cccctccctt
       61 cctctcccct ccccctctcc tcctcttcca cagacgcttg gagcgcgacg ccaacatgga
      121 gccggaggac ctgccatggc ctgacgagct ggaagaggag gaagaggagg aggaagagga
      181 agggaaggag gaggcggaga atgcatcagc ggcagcgacc gaggaggccc ggacctctga
      241 ggagactggg agactggaag aggaagagtt ggaaggggcg ggtccggagg tggacttgga
      301 ttttaacagc gagtcccagc tgcaagagag cacggacgaa gaggaggacg agctggctaa
      361 agcctggctg caggctcacc ctgaccgtcc tggccaaggt ttctctctga ccccgcctcc
      421 gactccgcct ccgtcctccc ggcaccagta caggccggta gagcactggg gcaagaggga
      481 ggttattcca ctgacttacc gtgcatggca gcagacatca taccaagata atgctagagc
      541 ccagttttcc aactccagta cattgttatt ggagccagaa tttcagtggg accctgagga
      601 ggttcagaga acagagtctt ggcatcatct tcctcaagaa agggattctt tccaaacctt
      661 gaatatatcc cagactggga tcgacagagt agaaggagca gaagtaaaag acttgccctc
      721 tcaggaggga ggtgaatcgg ttcaggcaca gtgtccagta aaggaaccaa agctggatct
      781 cttgtgttct ccattgctag tgatacagga tgactttgct gctcctgatt tgcccttgct
      841 gacatgtatg acccaagacc aggagtttga acctgattcc ttgtttcaac aaagtgaact
      901 ggaatttgca cctttgagag ggattcctga taagtctgaa gatattgaat ggcttgctcg
      961 accgtcagaa gttagtgaag ctttaatcca ggccacctca gaatcatctt cagacttagt
     1021 taacagttgc tttagtatat ctcagcatcc actcacagag ggcctgcagg ggaaggctaa
     1081 atctgatgtc cttattctgt ttggtgatgc taaatacgcc agcatatatg aaaatgctgg
     1141 tgcacacagt gaaagaatta ccgaattgca aagagaggta gactgttcta atctgagcat
     1201 tacccaggct tttccatctt ccttgccttc atttgttcct caagaaccga ccattgagtc
     1261 agaatatcat tcctccaatc tcagaatgtt gagagtatct cccgacactc tgctgacaac
     1321 tcttacacag acaacaggat ctgttgacca gaagattgga gcagctgcaa tctcttctcc
     1381 ttactcacat ggagtacagc ctggcagctt tccccaagaa gagttgccag ataggcatct
     1441 taccgaagag attcggaaag tgtcacctgc tcctaggcca gctggccaga agcctgagat
     1501 gccaacagta ccatctagtt cttactctaa gggaataaaa tctgttttct atcgatatcc
     1561 gatgtcatac agtcctcaag gcaaagagcc tccaaatgtt tcatctgttt ctggatcagc
     1621 tgagaatgcc ctgcccctaa cagtaacctc tgtttcccag ccacagagag agacagctaa
     1681 agctttctac cagcaaacat tatcagacag tcttctaact gcagaaacat tgccagtttc
     1741 tgttattcct gaatcaggtg accagaagac tcatttgcta tcagggttct ctctctcata
     1801 ttctcacaga ggagaggacc tgcctgaaga tgtagtgaaa gtttccactg attctggatc
     1861 tgttgacaag aaggctgaca ttccaacagt gtcctctagt acctaccagc acaaaacaaa
     1921 acctggtatt atttactacc aagagtttcc agacagccat gtgcctatag ggactagaaa
     1981 ggttgtatct cagtcaggag cagctggcca gaagactgat ctatcagcag tgccctctac
     2041 ttcctcttca caggcagaga agcctgttcc cccctccctg ctaactttgc taggtagtca
     2101 tctacctgaa gatgttttca aagcttctta tgtttgtaag tcatctgaag agccatctgg
     2161 aataacagct ctgacctctg tttcctactc atataaaggg agacccaata tttctcatca
     2221 agagaagttt ccagacagtt acctaattga agaggcccag aaaatcttgg gttcaactgt
     2281 aacagtagac cagaagacag tgacagcaaa agaaaagcct agtgttttct accaacaggc
     2341 cttgccagaa ggtggtctat ctgcagagga tctgcaagtt gaccagaaca ttgctatacc
     2401 aatggtaact tctgcttcct tctcacaaag agaaaaacct caggttttct accaagaggc
     2461 gctctcaggt ggtcatttgc ctggagagcc tcctggaccg cccgaccaga acactgaggc
     2521 accaacaata tcgcctagtt cctacttccc tggagaagag tccattattt tttaccaagc
     2581 tggctttcca ggtaacactc catctgtagt gcctttcaaa gtttcaagga tttctgaatt
     2641 agctgaacag aagactaaca taacaacagg accatcaggt tcctactcag ttggagagaa
     2701 gtccattatt ttctaccatc aggccttgcc agatggtcat ctttctaaag aagcttctcc
     2761 tgcaccagct gacctgaaga ctggggagcc accagtatat tcggcttcct gttctgttga
     2821 ggtaaagccc attattttct accagcagcc tctgtcggat agtcccctaa ctaaaggtca
     2881 caacgtttca gttgttcctg gaccaactga ccagaagact ggtataccag cagtgccttc
     2941 tacttcacag tcatatatag agaggcgttc tgtttcttac cagcaagagt ttccaaatgt
     3001 tagtgaaaaa gctttgaaag ttctaggtgg tgttagatcc actgaacaga aaactgaaat
     3061 accagtagtg tcttctgctc tcttacacac agaggtacct actatttctt cccaagagga
     3121 tttgccaaat cttactgaag aagctttgca aattttaggt gttgctgaag aagttccttc
     3181 tgtcccttca cgtgataaga cacgtctcat ctcttaccag aagaagttac cagatggtac
     3241 tgaagtatct ttgaaatcag taggctttgg ctctactgat cagaagactg gggcacacat
     3301 agtctcctcc tcaagaagag agaagtccag tggttttcat cagcaggagt tgccaaatac
     3361 ttgtggggat gcattaggta cttttcatcc tgaaccagtt gcccagaagg ttggaaaacc
     3421 aacagaacct tccaacatac agggaaaggc cagtgctttg tctccacagc agttgccggc
     3481 cagccatctt acaggagagg ctctgaaggt gtcagacggt tctgcctgtg tggcacagat
     3541 gcctgaggca ccagcaggac cgtccatttt ctacttccat aaagaaaggt tcagtaatta
     3601 tcaaaaggcc tcaccagatt gtgatctaac tgaaaattct ttgaagtctt caactgttcc
     3661 tggattgtct gatcagaaaa agagacctcc agtctcatct ggttcctgtt cacacaaaga
     3721 gaaacacaag atttcggctg cagatttgct ggatgcccag gcggctgaac tgccaacagt
     3781 tattcagaga tcctgcttac atagagaaaa gctggcaatt tcaactgtga tcaaaccaga
     3841 tgacccaaag attccattac caacaacttg tcatgcctcc tgtgctcaaa aagtaaaacc
     3901 tgttattttt gttcaaaaac agttatcagg tagagatcaa agtgaagata ttccgaagat
     3961 ttcaactgtc tctgagccaa ctgttgcaaa cacaggtgca ccagtacttc tgtctagttc
     4021 ttacccatac agagagaagt cagatttctc ttccccaaag gaactgccag atagacatcc
     4081 agctaaagtt gacatgcagg cttcaagcag tcttggacaa gctgaccaga tttctaggtt
     4141 acctacagtt cctcctggta cttattcaca cagtgaaaag taccagctta tctcagagca
     4201 tgtacaagag ctgattgata atttgaattc ttctgagtct tgtctcaatg cagacattat
     4261 gatgccttca aattcacaaa ttgatgatgg agttatcata tgtaagccag aatcttcacg
     4321 ttttgccaat gttggctgtg aggaaatcca ggatatagat catggttcca aaactcttaa
     4381 agaaattcag actctcttaa tggaagcaga aaatatggca ctaaaacgat gcaatttctc
     4441 tgttccctta gttcctttca gagatgttag tgatgtttca tttgtacaat caaagaaggt
     4501 ggtttgcttc aaagaaaccc ccacatctga tgtttgtatc cagaaggact catttgcaga
     4561 ggaagtccca cacactaagt atgtacagaa ggacatcggt acacagacga accttaagta
     4621 ccagaggaat gttagaaatt gggaatttat tagttcagct acctttagaa gtcctctaca
     4681 tgaagcaaat ggcacagctc gagtggcatt tgatgaaaat ttcaggcaat atagagctac
     4741 caggtctgta atgaggtctg aacctgaaga ctgcagcaca ggcattgggg ataaaattat
     4801 tatccctatg atgacaatca taaagagtga ctcgagtagt gatgtaagtg atggttgctg
     4861 ctcatgggac aataatttac cagagtcttt ggaatctgtt tctgatgttt ttctaaactt
     4921 ctttccgtat acttcaccca agacaagcat aacagatagc cgagaagagg agggcttgtc
     4981 agagagtgaa gatggctatg gtagtgcaga ttcattggct gcacatgtga agtaccttct
     5041 gcagtgtgaa tcctcactga atcaagccaa acagatactt aagaatgcag aagaggagga
     5101 atatcgagtc cgagcccaag cctggaatct aaagttcaat ttaggacgtg actatggata
     5161 ccccgtttca gagttaaatg aagatgacag gaggaaagta gaagagataa aggcaaagtt
     5221 gtttggtcat ggaagaacaa ctcacgtgtc tgaggtctta cagagtccac gaggaatagg
     5281 atgtttgcct gaagctgtat gtggtcacat tattattgag agccatgaga aaggatgtta
     5341 caggactctt actgctgaac aaccacgacc agacagctgc cattgtgttt ttcgatctgt
     5401 tgatccttca gacttgatta gaggacatcg gagcccatca tcatggagag gcaggcacat
     5461 caacctttcc agatcgatag atcagagcaa tccccatttc aaaataggga attcctttca
     5521 gttacagagt catcccccat ttgaaaaact tgtacctgat gacatcaaaa ttagcaaggg
     5581 tgttggaatg ccattccatg aacacatgga tccccagcca tcagaattaa tagaacctac
     5641 ttgtgtgcca gctaaagaaa tggatttttc ttcctcttct gaaatactgc cctctgaacc
     5701 caagaagcag ttcaccactt ccatcacttt ttcttctcac caacactcta agtgcatttc
     5761 tgattcctct ggttttaagg ttgatgttac tgcaggtagc cagtgtactg ggccatcttt
     5821 aggggtgttt aagcctcata tccctgaaga gcagatttct cctagagatc ttaaacagaa
     5881 aatctcgtcc ccttcatcac ttcaaaggca tagttacaca ccagtgacta ttttagttga
     5941 cagtagtagg caaagacaaa aattacctgt tgattttgag cattctaatc aaaaaggaaa
     6001 actcttacaa agatcagctt ttaattttag ccattctgaa cccagtgtca gtacaaatgt
     6061 tagtttcaag ggagttcaat tttctggtaa cgataccatc gttaggcagg acaaattaat
     6121 ttccacagtt gaggtaaaag agaagaatgt aactttaact cctgatcttc cttcttgcat
     6181 ttttcttgag caaggagaag aaagccacac cccacacaca gatcttcata tgaggaagtg
     6241 cccttctccc tctggtctag acattgcttc ttgcattttt cttgagcagc acaaattctt
     6301 tgagcaaagc caaggccagc atgtagatga tcatgaaatg agagaagact actctctctt
     6361 ccctaagtgc cagggttaca tagctgcaga cccttctgac tttcctgatc agcagcaatg
     6421 caaacctcca ggtgtagatg gccacgtgag aaaacagcac tatcctcttt ctccagttca
     6481 ggattgtgag gtagaagagg ttcagtgtat accccaatca tacttttcta atatggtaaa
     6541 tgttgaagcc aaggtcagtg atatggtctc ccctgacgcc cctgaccact ttacagcagc
     6601 atctactcca ccttcaagta gaaaagcact ttcatgtgtt cgcataactc tttgtcccaa
     6661 gactccttcc aagttggata gtggaacctt agacgaaagg tttcattcat tggatcctgc
     6721 ttctaaaaca aggattaata gtgagtttaa ctctgatctt cgaattatat cttcaaggtc
     6781 attggaacca acctccaaat tactgacctg taaaccagta gcacaggatc aagaatcttt
     6841 agtttttcta ggacctaaat ctccactgga cttgcaagtc gcagagtctt ctcttccaga
     6901 tagtaagact atttctcagg acttgaaaac cacacctcct cagaatagcc agatagtaac
     6961 ctcaaggcaa acacaagtga acatttcaga tttggaagaa tattccaaac cagaggggac
     7021 tccagtacct gcagatggtt cacaagaaca gaataaggct ccctttacta catcctctgg
     7081 aatgttatca tctgatgcag tcactcaggt aacaacagaa agtccagaaa agatgacatt
     7141 ttcatctgaa atttttatta atgctgatga tcgtggacag gacattccat accccacgac
     7201 ccaaaatcca tccaggtttg cctcctcttc ctcagggcag caaatcacag cttctcaggg
     7261 caaagacgcc cagcctgtgt tgttgccata taagccatct ggtagttcga agatgtacta
     7321 tgttccactg ttaaaaccag ttccgtcata tctggattcc aaatcagata ctactgttga
     7381 gagctcacat tcaggctcca atgatgctat tgccccagac tttccacctc aagtgctagg
     7441 cacaagagat gatgacctct ccaccactgt aaacattaag cacaaagaag ggatctatag
     7501 caagagggct gcatcaaagg ggaaacaacc ctctcagaaa ggtaatgcag aggccccagt
     7561 tcaaatgcct gtaacttggg ataagaatgt ctataaggaa aaccaagacg agatcatcag
     7621 tagaagagtt gtgatcgaga cggcaggacc tgaagaaatg ccatcccagg agaaggacct
     7681 tgcaggatcc agtgatatca ctgtgcaaga cagaaaaact ggaaatctgc cagacgctaa
     7741 atccattaaa cagaaggaag gatccttaga gatagagcct gagtgtcact ccgagtttgc
     7801 aaacaccact cactctgtct tcaggtcagc cagcttttac ttccaccacc cagtgcacct
     7861 gccccaagag caggatctct gccgcgagtc ttcgggaagg aatgttctca cgcagcatcc
     7921 ttggaaggat ctctttcact gtcattcagg gcacagcggc ctcccgcccc ctggcccaag
     7981 gtcagacaac actgagatgg agtgcaccag agttaagagc ctcagcatgg atttgaattt
     8041 ggggggccat gagaagatac atactatcaa gaatcaggcc agagacacaa aagaagacaa
     8101 aagaccggca aatgacccaa aaaaggatca gaaggtcacc ccagagctaa caagtcagtg
     8161 ccccatgagt ttgaatgaac tctggaacaa gtaccaggag cgacagaagc agcagaaacc
     8221 atctggcgct tgtgataaca aagagctctc cttggtggaa cggcttgatc gtttggctaa
     8281 actgcttcag aatcccatca cacattcact ccaggcctca gaaagtactc aagatgaccg
     8341 cagatgggga cgtggagcca gggggtggac tgggaggagg caacatcaac agaaaggcaa
     8401 gcagcagagg aagtggagta agagcctaga gaggggtcac agtacaggtg actttaggaa
     8461 aagcaaagtg ctttctcctc atgaaggtgg gaaatccagt cagcgtaata ttgagaagat
     8521 taaactagat aagtacatcc tgagaaagca gccagatttt aataatgtca gcaatacctc
     8581 cttggattct agaccctcag aggaaagtga gctcctcctc acagatagtc ccaacctttt
     8641 ttccagcact gagtctcctg tggagtcaga tgtattgacc cacactggta gggacatgcc
     8701 tttaaatgag aggagtagct ccatttctac cgttgacaca gcccggctga tccaagcctt
     8761 tggccacgat agactgtatt tgtcacccag gcaaattaaa ttatacagta gtgtcactaa
     8821 tcaacggagg aaacatctgg agaagccatg caagcacaag aggaaagcat tgaacacagc
     8881 ctgcccccaa atgacttctg agcacaccag aaggaggcac gtccaggtgg caaaccgtat
     8941 aatgtcttct gactctgttt catctgctgg tagttgtttg agcttggact ctactctgtc
     9001 caatgaggaa aatgtgcaca tgttgaataa gggtgtacaa gctggtaact tggagattgt
     9061 gactggtgcc aaaaaataca ctcaggatgt tggggtgact ttcccaacgc ccagttctag
     9121 tgaggccaga ttggaagagg atagtgatat gacttcttgg tcagaagaaa aagccaaaga
     9181 gaaaaagttc ctttccaact atctgcgggg caaaaactta aggaaaaaca agccaaatcc
     9241 ctgtgaagga gtctcatggt ttgttcctgt ggaaaacaga aagtctgaat ctaagaagga
     9301 aaacctgccc aagatttaca gacctgctat ctcctggttt gaaccagtaa cccaaaccaa
     9361 accctggagg gagccactga gggaacaaaa ctggcaggca caatgcatga acagtcgtgg
     9421 gtccctggga ggcccaggca gggatagtgg ccagagtttt cttcagccat ttgtgagagc
     9481 aaccctgcag gaatcacttc agcttcacag acctgatttc atctcccgct ctggggagcg
     9541 gataaaacgt ctgaagctac tagtccagga gaggaagcta cagaacctgt tccagaatga
     9601 gcgagaagct ctgttcaaca ctgcgaggcc cctgcccagg agagttctct tggcagtcca
     9661 gaagaacaag cctattggga agacggaaat gattcggagg accagacggt tttatgagca
     9721 gcttccagaa gtgaagaaga agagagaaga ggagaagaga aaatcagaat atacatccta
     9781 ccggctacga gctcagcact ataaaatgaa agtgaccaat catctcctgg ggagaaaagt
     9841 gccttgggac tgacataaga ctacttcaga gcctaggaat acttttttta tgagcccaaa
     9901 gaagctaatt tatactttaa tgggtgtgat ttaataaagt ggtgtctttg tctgtgtgca
     9961 cctta
//
