LOCUS       NM_001106259            1599 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus AKT1 substrate 1 (Akt1s1), mRNA.
ACCESSION   NM_001106259 XM_001078095 XM_238103
VERSION     NM_001106259.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Hao,J., Li,F., Liu,W., Liu,Q., Liu,S., Li,H. and Duan,H.
  TITLE     Phosphorylation of PRAS40-Thr246 involved in renal lipid
            accumulation of diabetes
  JOURNAL   J Cell Physiol 229 (8), 1069-1077 (2014)
   PUBMED   24347388
  REMARK    GeneRIF: These data suggested that phosphorylation of PRAS40-Thr246
            mediated abnormal lipid metabolism in kidney of diabetes and might
            be the potential target for treating lipogenesis of diabetic
            nephropathy.
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Xiong,X., Xie,R., Zhang,H., Gu,L., Xie,W., Cheng,M., Jian,Z.,
            Kovacina,K. and Zhao,H.
  TITLE     PRAS40 plays a pivotal role in protecting against stroke by linking
            the Akt and mTOR pathways
  JOURNAL   Neurobiol Dis 66, 43-52 (2014)
   PUBMED   24583056
  REMARK    GeneRIF: PRAS40 appears to reduce brain injury following ischemic
            stroke by converting cell signaling from Akt to mTOR
REFERENCE   3  (bases 1 to 1599)
  AUTHORS   Wang,H., Zhang,Q., Zhang,L., Little,P.J., Xie,X., Meng,Q., Ren,Y.,
            Zhou,L., Gao,G., Quirion,R. and Zheng,W.
  TITLE     Insulin-like growth factor-1 induces the phosphorylation of PRAS40
            via the PI3K/Akt signaling pathway in PC12 cells
  JOURNAL   Neurosci Lett 516 (1), 105-109 (2012)
   PUBMED   22490886
  REMARK    GeneRIF: Taken together, these data demonstrate that IGF-1
            stimulates the phosphorylation of PRAS40 at Thr246 in neuronal
            cells and the effect of IGF-1 is mediated, at least in part, by the
            PI3K/Akt signaling pathway.
REFERENCE   4  (bases 1 to 1599)
  AUTHORS   Laplante,M. and Sabatini,D.M.
  TITLE     mTOR signaling in growth control and disease
  JOURNAL   Cell 149 (2), 274-293 (2012)
   PUBMED   22500797
  REMARK    Review article
REFERENCE   5  (bases 1 to 1599)
  AUTHORS   Dey,N., Ghosh-Choudhury,N., Das,F., Li,X., Venkatesan,B.,
            Barnes,J.L., Kasinath,B.S. and Ghosh Choudhury,G.
  TITLE     PRAS40 acts as a nodal regulator of high glucose-induced TORC1
            activation in glomerular mesangial cell hypertrophy
  JOURNAL   J Cell Physiol 225 (1), 27-41 (2010)
   PUBMED   20629086
  REMARK    GeneRIF: Data identify high glucose-induced phosphorylation and
            inactivation of PRAS40 as a central node for mesangial cell
            hypertrophy in diabetic nephropathy.
REFERENCE   6  (bases 1 to 1599)
  AUTHORS   Yu,F., Narasimhan,P., Saito,A., Liu,J. and Chan,P.H.
  TITLE     Increased expression of a proline-rich Akt substrate (PRAS40) in
            human copper/zinc-superoxide dismutase transgenic rats protects
            motor neurons from death after spinal cord injury
  JOURNAL   J Cereb Blood Flow Metab 28 (1), 44-52 (2008)
   PUBMED   17457363
  REMARK    GeneRIF: overexpression of SOD1 in the Tg rats results in an
            increase in endogenous pPRAS40 and a decrease in motor neuron death
            through the PI3K/Akt pathway.
REFERENCE   7  (bases 1 to 1599)
  AUTHORS   Sancak,Y., Thoreen,C.C., Peterson,T.R., Lindquist,R.A., Kang,S.A.,
            Spooner,E., Carr,S.A. and Sabatini,D.M.
  TITLE     PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein
            kinase
  JOURNAL   Mol Cell 25 (6), 903-915 (2007)
   PUBMED   17386266
REFERENCE   8  (bases 1 to 1599)
  AUTHORS   Nascimento,E.B., Fodor,M., van der Zon,G.C., Jazet,I.M.,
            Meinders,A.E., Voshol,P.J., Vlasblom,R., Baan,B., Eckel,J.,
            Maassen,J.A., Diamant,M. and Ouwens,D.M.
  TITLE     Insulin-mediated phosphorylation of the proline-rich Akt substrate
            PRAS40 is impaired in insulin target tissues of high-fat diet-fed
            rats
  JOURNAL   Diabetes 55 (12), 3221-3228 (2006)
   PUBMED   17130464
  REMARK    GeneRIF: effect of in vivo insulin action on phosphorylation of the
            PKB/Akt substrate 40
REFERENCE   9  (bases 1 to 1599)
  AUTHORS   Saito,A., Hayashi,T., Okuno,S., Nishi,T. and Chan,P.H.
  TITLE     Modulation of proline-rich akt substrate survival signaling
            pathways by oxidative stress in mouse brains after transient focal
            cerebral ischemia
  JOURNAL   Stroke 37 (2), 513-517 (2006)
   PUBMED   16397181
REFERENCE   10 (bases 1 to 1599)
  AUTHORS   Saito,A., Narasimhan,P., Hayashi,T., Okuno,S., Ferrand-Drake,M. and
            Chan,P.H.
  TITLE     Neuroprotective role of a proline-rich Akt substrate in apoptotic
            neuronal cell death after stroke: relationships with nerve growth
            factor
  JOURNAL   J Neurosci 24 (7), 1584-1593 (2004)
   PUBMED   14973226
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FM032217.1, EV773331.1, JAXUCZ010000001.1 and BI276211.1.
            
            On Oct 12, 2012 this sequence version replaced NM_001106259.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments and orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643292.2021.1,
                                           SRR26643299.2315.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-385               FM032217.1         1-385
            386-823             EV773331.1         302-739
            824-1263            JAXUCZ010000001.1  104475410-104475849
            1264-1599           BI276211.1         1-336               c
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q22"
     gene            1..1599
                     /gene="Akt1s1"
                     /gene_synonym="PRAS40"
                     /note="AKT1 substrate 1"
                     /db_xref="GeneID:292887"
                     /db_xref="RGD:1312049"
     exon            1..111
                     /gene="Akt1s1"
                     /gene_synonym="PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            112..500
                     /gene="Akt1s1"
                     /gene_synonym="PRAS40"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..892
                     /gene="Akt1s1"
                     /gene_synonym="PRAS40"
                     /note="PKB/Akt substrate 40; AKT1 substrate 1
                     (proline-rich)"
                     /codon_start=1
                     /product="proline-rich AKT1 substrate 1"
                     /protein_id="NP_001099729.2"
                     /db_xref="GeneID:292887"
                     /db_xref="RGD:1312049"
                     /translation="MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPC
                     AYAAHGRGALAEAARRCLHDIAQAHRAATATRPPGPPPAPQPPSPAPSSPPRPALARE
                     EDEEDEDEPTETETSGERLGGSDNGGLFMMDEDATLQDLPPFCESDPESTDDGSLSEE
                     TPAGPPAYPKLPATALPTQQYAKSLPVSVPVWAFKEKRTEARSSDEENGPPSSPDLDR
                     IAASMRALVLREAEDNQVFGDLPRPRLNTSDFQKLKRKY"
     exon            501..578
                     /gene="Akt1s1"
                     /gene_synonym="PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            579..748
                     /gene="Akt1s1"
                     /gene_synonym="PRAS40"
                     /inference="alignment:Splign:2.1.0"
     exon            749..1582
                     /gene="Akt1s1"
                     /gene_synonym="PRAS40"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgaggacaaa ccatagaggc ttgttgggag ccatgttaat gcggggcact atcatctctt
       61 tgcggggcac gacggcgagg tgttggaaaa gcgaaacgga gaaaggaaaa gggctcggat
      121 ggcgtctggg cggccagagg aactgtggga agccgttgtg ggtgccgccg agcgctttcg
      181 ggcccgcact ggcacggagt tggtgttact gacagcagca ccgccgccgc cgccccgccc
      241 agggccctgt gcctatgctg cccatggccg aggagccctg gcagaggccg cccgccgctg
      301 cctccacgac atcgcccagg cacacagggc tgccactgcc acccgacctc ctggtccccc
      361 accagcacca cagccaccca gccccgctcc tagctcacca cctcggccag ccttggccag
      421 ggaggaagat gaggaagatg aagacgagcc cactgaaaca gagacatctg gagagcggct
      481 gggcggtagc gataatggag gtctcttcat gatggatgag gatgccaccc tccaggacct
      541 tccccccttc tgcgagtcag acccggaaag cacagatgac ggcagcctga gtgaggagac
      601 acccgccggt cccccagcct accccaaact cccggctaca gccctgccta cccagcagta
      661 tgccaagtct ctgcccgtgt cggtgccagt gtgggccttc aaggagaaga ggacagaagc
      721 ccgatcgtca gatgaggaga atggcccgcc ctcctcgccc gacctagacc gaatagcggc
      781 tagcatgcgc gcgctagtgc tgcgggaggc tgaggacaat caggtcttcg gggaccttcc
      841 gcggccgcgg ctcaatacca gcgactttca gaagctgaag cgaaaatatt aagcgtgcag
      901 aatgcctcgg cacacgcgag gccactgtct gctctgccca gcaacagaac aattctttac
      961 gggattggct cgctccgatt gggcggggct agttctaaac tctcagaggg tcaactccgt
     1021 ttcttgccta gcaacagaag ctttgcccgc acagccttga ctcgcggccc cagggcggag
     1081 cccagctgcg ggagctggct ctgctcagcc agtgtccgaa gagttgccag cttccgttcc
     1141 agagttccct tgtccgattg gtcccggcct cctgggagtg tggccaagcc ctccctaacc
     1201 cagaattggc cggaggcctt atatttctta cactactagg gctgttaatg aatctttttt
     1261 ttgtttttgt ttttgttttt ttcttttttc ctttgcctga gtcctgacat ccggtcccct
     1321 aggggacagt ccagtcctcc tctgcgtcta acgcccctat aggtttcacc gcacgacagc
     1381 ctgtccatca aaacttgtcc tgtacccagg acagtaccag cttcctgtcc cccgacccta
     1441 acaggtgcct taaaaggccc tctcccaccc aaggtggggg ggccaggggg cctcattttc
     1501 tggccctgga cttgggtgcg ggagagtggg atggggatcg ggaagggcgc tctgagatta
     1561 aagttttacc tctgactggt gaaaaaaaaa aaaaaaaaa
//
