LOCUS       NM_173136                993 bp    mRNA    linear   ROD 20-MAR-2023
DEFINITION  Rattus norvegicus aldo-keto reductase family 1, member B8 (Akr1b8),
            mRNA.
ACCESSION   NM_173136
VERSION     NM_173136.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Sundaram K, Dhagat U, Endo S, Chung R, Matsunaga T, Hara A and
            El-Kabbani O.
  TITLE     Structure of rat aldose reductase-like protein AKR1B14 holoenzyme:
            Probing the role of His269 in coenzyme binding by site-directed
            mutagenesis
  JOURNAL   Bioorg Med Chem Lett 21 (2), 801-804 (2011)
   PUBMED   21168333
  REMARK    GeneRIF: The crystal structure of the binary complex aldose
            reductase-like protein-NADPH was determined at 1.86A resolution.
REFERENCE   2  (bases 1 to 993)
  AUTHORS   Zeindl-Eberhart E, Klugbauer S, Dimitrijevic N, Jungblut PR, Lamer
            S and Rabes HM.
  TITLE     Proteome analysis of rat hepatomas: carcinogen-dependent
            tumor-associated protein variants
  JOURNAL   Electrophoresis 22 (14), 3009-3018 (2001)
   PUBMED   11565795
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AJ277957.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ277957.1, SRR8487226.111853.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q22"
     gene            1..993
                     /gene="Akr1b8"
                     /note="aldo-keto reductase family 1, member B8"
                     /db_xref="GeneID:286921"
                     /db_xref="RGD:708475"
     exon            1..108
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..993
                     /gene="Akr1b8"
                     /EC_number="1.1.1.21"
                     /function="detoxification of xenobiotic and endogenous
                     aldehydes and ketones"
                     /note="aldose reductase-like protein"
                     /codon_start=1
                     /product="aldose reductase-related protein 2"
                     /protein_id="NP_775159.1"
                     /db_xref="GeneID:286921"
                     /db_xref="RGD:708475"
                     /translation="MATFVELSTKAKMPIVGLGTWKSMPNQVKEAVKAAIDAGYRHID
                     CAYAYCNENEVGEAIQEKIKEKAVRREDLFIVSKLWPTCFEKKLLKEAFQKTLTDLKL
                     DYLDLYLIHWPQGFQAGKELFPKDEQGNVLPSKTTFLEAWEGMEELVDQGLVKALGVS
                     NFNHFQIERLLNKPGLKHKPVTNQVECHPYLTQEKLIQYCHSKGIVVTAYSPLGSPDR
                     PRAKPDDPSLLQDPKIKEIAAKHKKTTAQVLIRFHIQRNVVVIPKSVTPARIQENIQV
                     FDFQLSDQEMATILSFNRNWRACLLPETVNMEEFPYDAEY"
     exon            109..276
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     exon            277..393
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     exon            394..471
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     exon            472..594
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     exon            595..701
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     exon            702..783
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     exon            784..867
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     exon            868..950
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
     exon            951..993
                     /gene="Akr1b8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggacacaga cctctgctct cacaaagatt tcagcaacaa ccatggcaac cttcgtggag
       61 ctcagtacca aagccaagat gcccattgtg ggtctgggca cctggaagtc tatgccaaac
      121 caagtcaagg aagctgtgaa ggcagccatt gatgctgggt atcgacatat tgactgtgcg
      181 tatgcatatt gcaacgagaa tgaggtgggg gaagccatcc aagagaagat caaagagaag
      241 gctgtgaggc gggaggacct cttcattgtc agcaagttgt ggcccacctg ctttgagaaa
      301 aaactgctaa aggaagcctt tcagaagacc ctcacggatc tcaagctgga ctacttggac
      361 ctctatctta tccactggcc tcagggattt caggcaggga aggagttatt ccccaaagat
      421 gaacaaggca acgtcctccc cagtaaaaca acgttcctgg aagcctggga gggcatggag
      481 gaactggtgg accaggggct ggtgaaagct ctgggcgtct ccaacttcaa ccacttccag
      541 atcgaaaggc tcctgaacaa gcctggactg aaacataagc cagtgaccaa ccaggtcgag
      601 tgccaccctt acctcaccca ggaaaaactg atccagtact gtcactccaa gggcatcgtg
      661 gtcacagcct acagtcccct gggctcccca gacaggccta gggccaagcc agatgaccct
      721 tcactattac aggaccccaa gattaaagag attgccgcca agcacaagaa aaccacagcc
      781 caggttctta ttcggttcca catccagagg aacgtggtag tgatccccaa gtctgtgacg
      841 ccagcacgta tacaggagaa cattcaggtc tttgacttcc agttgagcga ccaggagatg
      901 gccactatcc tgagcttcaa cagaaactgg agggcctgcc tgctgcctga gacagtcaac
      961 atggaagagt ttccctatga cgcagagtac tga
//
