LOCUS       NM_053503               3261 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus ajuba LIM protein (Ajuba), mRNA.
ACCESSION   NM_053503 XM_341313
VERSION     NM_053503.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Foxler,D.E., Bridge,K.S., James,V., Webb,T.M., Mee,M., Wong,S.C.,
            Feng,Y., Constantin-Teodosiu,D., Petursdottir,T.E., Bjornsson,J.,
            Ingvarsson,S., Ratcliffe,P.J., Longmore,G.D. and Sharp,T.V.
  TITLE     The LIMD1 protein bridges an association between the prolyl
            hydroxylases and VHL to repress HIF-1 activity
  JOURNAL   Nat Cell Biol 14 (2), 201-208 (2012)
   PUBMED   22286099
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   James,V., Zhang,Y., Foxler,D.E., de Moor,C.H., Kong,Y.W.,
            Webb,T.M., Self,T.J., Feng,Y., Lagos,D., Chu,C.Y., Rana,T.M.,
            Morley,S.J., Longmore,G.D., Bushell,M. and Sharp,T.V.
  TITLE     LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for
            microRNA-mediated gene silencing
  JOURNAL   Proc Natl Acad Sci U S A 107 (28), 12499-12504 (2010)
   PUBMED   20616046
REFERENCE   3  (bases 1 to 3261)
  AUTHORS   Das Thakur,M., Feng,Y., Jagannathan,R., Seppa,M.J., Skeath,J.B. and
            Longmore,G.D.
  TITLE     Ajuba LIM proteins are negative regulators of the Hippo signaling
            pathway
  JOURNAL   Curr Biol 20 (7), 657-662 (2010)
   PUBMED   20303269
REFERENCE   4  (bases 1 to 3261)
  AUTHORS   Zheng,P. and Dean,J.
  TITLE     Role of Filia, a maternal effect gene, in maintaining euploidy
            during cleavage-stage mouse embryogenesis
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7473-7478 (2009)
   PUBMED   19376971
REFERENCE   5  (bases 1 to 3261)
  AUTHORS   Montoya-Durango,D.E., Velu,C.S., Kazanjian,A., Rojas,M.E.,
            Jay,C.M., Longmore,G.D. and Grimes,H.L.
  TITLE     Ajuba functions as a histone deacetylase-dependent co-repressor for
            autoregulation of the growth factor-independent-1 transcription
            factor
  JOURNAL   J Biol Chem 283 (46), 32056-32065 (2008)
   PUBMED   18805794
REFERENCE   6  (bases 1 to 3261)
  AUTHORS   Kisseleva,M., Feng,Y., Ward,M., Song,C., Anderson,R.A. and
            Longmore,G.D.
  TITLE     The LIM protein Ajuba regulates phosphatidylinositol
            4,5-bisphosphate levels in migrating cells through an interaction
            with and activation of PIPKI alpha
  JOURNAL   Mol Cell Biol 25 (10), 3956-3966 (2005)
   PUBMED   15870270
REFERENCE   7  (bases 1 to 3261)
  AUTHORS   Pratt,S.J., Epple,H., Ward,M., Feng,Y., Braga,V.M. and
            Longmore,G.D.
  TITLE     The LIM protein Ajuba influences p130Cas localization and Rac1
            activity during cell migration
  JOURNAL   J Cell Biol 168 (5), 813-824 (2005)
   PUBMED   15728191
REFERENCE   8  (bases 1 to 3261)
  AUTHORS   Marie,H., Pratt,S.J., Betson,M., Epple,H., Kittler,J.T., Meek,L.,
            Moss,S.J., Troyanovsky,S., Attwell,D., Longmore,G.D. and Braga,V.M.
  TITLE     The LIM protein Ajuba is recruited to cadherin-dependent cell
            junctions through an association with alpha-catenin
  JOURNAL   J Biol Chem 278 (2), 1220-1228 (2003)
   PUBMED   12417594
REFERENCE   9  (bases 1 to 3261)
  AUTHORS   Marie,H., Billups,D., Bedford,F.K., Dumoulin,A., Goyal,R.K.,
            Longmore,G.D., Moss,S.J. and Attwell,D.
  TITLE     The amino terminus of the glial glutamate transporter GLT-1
            interacts with the LIM protein Ajuba
  JOURNAL   Mol Cell Neurosci 19 (2), 152-164 (2002)
   PUBMED   11860269
  REMARK    GeneRIF: We propose that Ajuba is a scaffolding protein allowing
            GLT-1 to regulate intracellular signaling or interact with the
            cytoskeleton.
REFERENCE   10 (bases 1 to 3261)
  AUTHORS   Goyal,R.K., Lin,P., Kanungo,J., Payne,A.S., Muslin,A.J. and
            Longmore,G.D.
  TITLE     Ajuba, a novel LIM protein, interacts with Grb2, augments
            mitogen-activated protein kinase activity in fibroblasts, and
            promotes meiotic maturation of Xenopus oocytes in a Grb2- and
            Ras-dependent manner
  JOURNAL   Mol Cell Biol 19 (6), 4379-4389 (1999)
   PUBMED   10330178
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC085802.1.
            
            On Nov 22, 2004 this sequence version replaced XM_341313.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC085802.1, AJ306292.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676787 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p13"
     gene            1..3261
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /note="ajuba LIM protein"
                     /db_xref="GeneID:85265"
                     /db_xref="RGD:620407"
     exon            1..1358
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /inference="alignment:Splign:2.1.0"
     CDS             323..1969
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /note="protein ajuba; ajuba homolog; jub, ajuba homolog"
                     /codon_start=1
                     /product="LIM domain-containing protein ajuba"
                     /protein_id="NP_445955.1"
                     /db_xref="GeneID:85265"
                     /db_xref="RGD:620407"
                     /translation="MERLGEKASRLLEKLRLSDSGSAKFGRRKGEASRSGSDGTPGAG
                     KGRLSGLGGPRKSGHRGANGGPGDEALEPAREQGPLDAERNARGSFEAQRFEGSFPGG
                     PPPTRALPLPLSSPPDFRLETTAPALSPRSSFASSSASDASKPSSPRGSLLLDGAGAS
                     GAGGSRPCSNRTSGISMGYDQRHGSPLPAGPCLFGLPLTTAPSGYSSGGVPSAYPELH
                     AALDRLCAHRPVGFGCQESRHSYPPALGSPGALTGAVVGTAGPLERRGTQPGRHSVTG
                     YGDCAAGARYQDELTALLRLTVATGGREAGARGEPLGIEPSGLEESPGSFVPEASRSR
                     IREPEAREDYFGTCIKCNKGIYGQSNACQALDSLYHTQCFVCCSCGRTLRCKAFYSVN
                     GSVYCEEDYLFSGFQEAAEKCCVCGHLILEKILQAMGKSYHPGCFRCIVCNKCLDGVP
                     FTVDFSNQVYCVTDYHKNYAPKCAACGQPILPSEGCEDIVRVISMDRDYHFECYHCED
                     CRMQLSDEEGCCCFPLDGHLLCHGCHMQRLSARQPPTNYI"
     exon            1359..1460
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1528
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1591
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1722
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1774
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1843
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..3234
                     /gene="Ajuba"
                     /gene_synonym="Jub"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggcagacaaa agtttggaga agttgggtcg gtgcaagtga gagccagggg ggcgggccgg
       61 gcagccgggc ggtggcgcga gccgacgcgt ggacagacct gcgaagacag cggctaccgt
      121 gggccctgcc agccacgcgc ggaccctctc gctggggcat gcttcgggat cgcgggcgcc
      181 cggcacctgg gccgttcgct gagccccggt gcgtggcttg catcccagga atccgagccc
      241 cggggaaccc gagaccggcc tctggccgaa agagtgcagc gtgcccggcg aggagggggc
      301 tgcaggcccc agatccgtgg gcatggagcg gttaggggag aaagccagtc gtctgctaga
      361 aaagttgaga ctttcggact ccggcagcgc caagttcggc cgcagaaagg gcgaagcgag
      421 ccggtctggg tctgatggga cccccggagc aggcaaggga cgcctgagtg ggctaggggg
      481 acctaggaaa tcaggacacc gaggagcgaa tggggggcct ggggatgaag ctttggaacc
      541 ggccagggag caagggcccc tggacgccga gcggaacgca cgcggctcct ttgaagcgca
      601 gcgcttcgaa gggtcctttc ccggggggcc gccgcccact cgagccttgc ctctgcccct
      661 gtcgtcgcct cctgattttc ggttggagac cacggctcca gcccttagcc ctcgctccag
      721 cttcgccagt agctcggcca gcgatgcgag caagccatcc agcccccggg gcagcctgct
      781 gctggacgga gcgggggcca gcggagccgg tggtagccgc ccgtgcagca atcggaccag
      841 cggcatcagc atgggctacg accagcgcca cgggagcccg ctgcctgcgg ggccgtgcct
      901 atttggcctc cccctgacca ctgctccttc gggctactcc tccggagggg ttccatccgc
      961 ctatccggaa ctccacgctg ccctcgaccg actatgcgct catcggccgg tgggtttcgg
     1021 ctgccaggag agccgtcact cttacccccc ggccctgggc agccccggag ctctaaccgg
     1081 agccgtggtg ggaacagcgg gtcctttgga gagacgtggg acgcaacccg gacgtcactc
     1141 ggttacgggc tacggggact gcgccgcggg agcccgttac caggacgagc taacagcact
     1201 gctgcgtttg acggtggcta ccggtgggcg agaagccggt gcccgcgggg aacccttggg
     1261 gattgagccg tcgggtctgg aggagtctcc tggttccttc gttccagagg cctcccgatc
     1321 ccggatacgg gagccagagg ccagagaaga ttactttggc acctgtatca agtgcaacaa
     1381 aggtatctat gggcagagca atgcctgcca ggccctggac agcctctacc acacccagtg
     1441 ctttgtctgc tgctcctgtg gacgaacttt gcggtgcaag gctttctaca gcgtcaacgg
     1501 ctctgtctac tgtgaggaag attatctgtt ttcagggttt caggaggcag ctgagaagtg
     1561 ctgtgtctgt ggccacttga ttctagagaa gatcctccag gccatgggga agtcctatca
     1621 tccaggctgc ttccggtgca tcgtttgtaa caagtgcctg gacggcgtcc ccttcactgt
     1681 ggacttctcc aaccaggtgt actgtgtgac tgactaccac aaaaattacg ccccgaaatg
     1741 tgcagcctgt ggacagccca tcctcccctc agagggctgt gaggacattg tgagggtgat
     1801 atccatggac cgtgattatc actttgagtg ctaccactgt gaggactgcc gcatgcagct
     1861 gagtgacgag gaaggttgct gctgtttccc ccttgacgga catttgctct gccacggctg
     1921 tcacatgcag cgcctcagtg cccgacagcc ccctaccaac tatatctgag ctgtagttac
     1981 tgctgccatc accactgttg agaaattctc tggccagctg aggcaaggag catgctctac
     2041 aatccttgtg aagaggtctc tggggaggcc ccgaaggcca gacacccaaa gatcctaaca
     2101 ttccaaatga cctgtggagg aggaaccttc ttcgcgggca gcctgtgcta cagcagatgt
     2161 gatgtggcag gcacggtggg tttcagtact tcctgaacat cagattataa tgtatctttt
     2221 cagtgtatgt ttctgtacca gtcaaaacaa tgttccggtg ctggatcaca tgagagggca
     2281 tcacagaaac caccccagtc tgttgtaccc ttcacatagg gttagcttgg cctttcccac
     2341 agtatcctat ctttgtctgc atgaagggct ggaagccttg gacttcatga tgagtcattt
     2401 cttggctatc tccctaagga ctagagagct cccccatctg tctgctggga ctgagaccag
     2461 ctctgggggc aggatggggt gtgccttgct gcctgtgttg cctgtttctc agggacctgg
     2521 ggaggctaac tggggcctgt ccttggttgc tgttttgttc cgctggtaat gagcaggttt
     2581 ctggatgtga aacttgcccc atgaataggc tgtcccggaa gcttccccca ctcccggggt
     2641 ttaagtgcac tgagccctct agccgagcat atgtggatac attgtatccg tgtgagcctg
     2701 tgcatctccc tcctgtactc tttttctcag gtgatttaca cttagttccc tagcagtagc
     2761 ctatcttttg agacccattt cttaaaggga tcctgcactg gatctgatct gcttaccacc
     2821 ccacaactca aggctttggg gaagctggag agaagaaaat aggttgactt ggttctagcc
     2881 acttggctga aacttgaatt caccttaagc cggaggactt aaagccagga cccttgctgg
     2941 ctgaaagtta ggttccttgg atgaccaggt tgccaagagt tccacccact atggagtgtt
     3001 tttatagctg cctaaacgtt ctgctcttct cgttacactg agcgttcagt ggtttctgcc
     3061 tgttcatcaa gagtccttca gagctattag caacctacat ttatcaactt tgtggcactt
     3121 tgtggtacac ttgcaccctg ccgtcacgtc ctgggtgtct tcctatgcta tcacttctct
     3181 tgacacttaa aaaaaaaagt ttaaattcta gcaataaata caattgataa tacaaaaaaa
     3241 aaaaaaaaaa aaaaaaaaaa a
//
