LOCUS       NM_030656               1628 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus alanine--glyoxylate aminotransferase (Agxt),
            transcript variant 1, mRNA.
ACCESSION   NM_030656
VERSION     NM_030656.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Falix,F.A., Weeda,V.B., Labruyere,W.T., Poncy,A., de Waart,D.R.,
            Hakvoort,T.B., Lemaigre,F., Gaemers,I.C., Aronson,D.C. and
            Lamers,W.H.
  TITLE     Hepatic Notch2 deficiency leads to bile duct agenesis perinatally
            and secondary bile duct formation after weaning
  JOURNAL   Dev Biol 396 (2), 201-213 (2014)
   PUBMED   25446530
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Montioli,R., Fargue,S., Lewin,J., Zamparelli,C., Danpure,C.J.,
            Borri Voltattorni,C. and Cellini,B.
  TITLE     The N-terminal extension is essential for the formation of the
            active dimeric structure of liver peroxisomal alanine:glyoxylate
            aminotransferase
  JOURNAL   Int J Biochem Cell Biol 44 (3), 536-546 (2012)
   PUBMED   22198249
REFERENCE   3  (bases 1 to 1628)
  AUTHORS   Fodor,K., Wolf,J., Erdmann,R., Schliebs,W. and Wilmanns,M.
  TITLE     Molecular requirements for peroxisomal targeting of
            alanine-glyoxylate aminotransferase as an essential determinant in
            primary hyperoxaluria type 1
  JOURNAL   PLoS Biol 10 (4), e1001309 (2012)
   PUBMED   22529745
REFERENCE   4  (bases 1 to 1628)
  AUTHORS   Ghosh,D. and Berg,J.M.
  TITLE     A proteome-wide perspective on peroxisome targeting signal
            1(PTS1)-Pex5p affinities
  JOURNAL   J Am Chem Soc 132 (11), 3973-3979 (2010)
   PUBMED   20178365
REFERENCE   5  (bases 1 to 1628)
  AUTHORS   Danpure,C.J., Fryer,P., Jennings,P.R., Allsop,J., Griffiths,S. and
            Cunningham,A.
  TITLE     Evolution of alanine:glyoxylate aminotransferase 1 peroxisomal and
            mitochondrial targeting. A survey of its subcellular distribution
            in the livers of various representatives of the classes Mammalia,
            Aves and Amphibia
  JOURNAL   Eur J Cell Biol 64 (2), 295-313 (1994)
   PUBMED   7813517
REFERENCE   6  (bases 1 to 1628)
  AUTHORS   Nishiyama,K., Funai,T., Katafuchi,R., Hattori,F., Onoyama,K. and
            Ichiyama,A.
  TITLE     Primary hyperoxaluria type I due to a point mutation of T to C in
            the coding region of the serine:pyruvate aminotransferase gene
  JOURNAL   Biochem Biophys Res Commun 176 (3), 1093-1099 (1991)
   PUBMED   2039493
REFERENCE   7  (bases 1 to 1628)
  AUTHORS   Purdue,P.E., Takada,Y. and Danpure,C.J.
  TITLE     Identification of mutations associated with
            peroxisome-to-mitochondrion mistargeting of alanine/glyoxylate
            aminotransferase in primary hyperoxaluria type 1
  JOURNAL   J Cell Biol 111 (6 Pt 1), 2341-2351 (1990)
   PUBMED   1703535
REFERENCE   8  (bases 1 to 1628)
  AUTHORS   Oda,T., Funai,T. and Ichiyama,A.
  TITLE     Generation from a single gene of two mRNAs that encode the
            mitochondrial and peroxisomal serine:pyruvate aminotransferase of
            rat liver
  JOURNAL   J Biol Chem 265 (13), 7513-7519 (1990)
   PUBMED   2332438
REFERENCE   9  (bases 1 to 1628)
  AUTHORS   Hoshino,J., Kuhne,U., Filjak,B. and Kroger,H.
  TITLE     Regulation and characterization of L-serine: pyruvate
            aminotransferase in rat liver cytosol and mitochondria
  JOURNAL   Z Naturforsch C Biosci 32 (3-4), 249-253 (1977)
   PUBMED   17958
REFERENCE   10 (bases 1 to 1628)
  AUTHORS   Hoshino,J., Kuhne,U., Filjak,B. and Kroger,H.
  TITLE     Inhibitory effect of cortisone acetate on the stimulation of rat
            liver cytosol L-serrine. Pyruvate aminotransferase by dibutyryl
            adenosine 3,5-monophosphate
  JOURNAL   Biochim Biophys Acta 399 (1), 42-49 (1975)
   PUBMED   167860
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from M35270.1 and BC088133.1.
            
            On Jul 25, 2012 this sequence version replaced NM_030656.1.
            
            Summary: This gene encodes alanine-glyoxylate aminotransferase,
            which catalyzes the interconversion of L-alanine and glyoxylate to
            pyruvate and glycine. Two alternatively spliced transcript variants
            encoding distinct isoforms have been found for this gene. The
            longer transcript variant includes an upstream translation start
            codon and a downstream translation start codon. The upstream start
            codon initiates the translation of the mitochondrial enzyme
            precursor while the downstream start codon initiates the
            translation of the peroxisomal enzyme (see PMID:2332438). [provided
            by RefSeq, Feb 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. It contains an upstream start codon and a downstream
            start codon. The upstream start codon initiates the translation of
            the longest isoform (1), which is a mitochondrial enzyme precursor.
            The mature peptide, also known as the mitochondrial enzyme, is
            identical to isoform 3, also known as the peroxisomal enzyme.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M35270.1, BC088133.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1595              M35270.1           1-1595
            1596-1628           BC088133.1         1428-1460
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q36"
     gene            1..1628
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /note="alanine--glyoxylate aminotransferase"
                     /db_xref="GeneID:24792"
                     /db_xref="RGD:2073"
     exon            1..339
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     CDS             109..1353
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /EC_number="2.6.1.44"
                     /EC_number="2.6.1.51"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; serine:pyruvate aminotransferase SPT;
                     angiotensin receptor 2; alanine--glyoxylate and
                     serine--pyruvate aminotransferase;
                     serine:pyruvate/alanine:glyoxylate aminotransferase;
                     serine-pyruvate aminotransferase; serine--pyruvate
                     aminotransferase, mitochondrial; serine--pyruvate
                     aminotransferase, peroxisomal"
                     /codon_start=1
                     /product="alanine--glyoxylate aminotransferase isoform 1
                     precursor"
                     /protein_id="NP_085914.2"
                     /db_xref="GeneID:24792"
                     /db_xref="RGD:2073"
                     /translation="MFRMLAKASVTLGSRAASWVRNMGSHQLLVPPPEALSKPLSIPK
                     RLLLGPGPSNLAPRVLAAGSLRMIGHMQKEMFQIMDEIKQGIQYVFQTRNPLTLVVSG
                     SGHCAMETALFNLLEPGDSFLVGTNGIWGIRAAEIAERIGARVHQMIKKPGEHYTLQE
                     VEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYQCLLLVDSVASLGGVPIYMD
                     QQGIDILYSGSQKVLNAPPGISLISFNDKAKSKVYSRKTKPVSFYTDITYLSKLWGCE
                     GKTRVIHHTLPVISLYCLRESLALISEQGLENSWRRHREATAHLHKCLRELGLKFFVK
                     DPEIRLPTITTVTVPAGYNWRDIVSYVLDHFNIEISGGLGPSEDKVLRIGLLGYNATT
                     ENADRVAEALREALQHCPKNKL"
     exon            340..532
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            533..597
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            598..698
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            699..769
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            770..854
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            855..950
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1020
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1116
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1245
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     exon            1246..1600
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1577..1582
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
     polyA_site      1600
                     /gene="Agxt"
                     /gene_synonym="AGT; Spat; SPT"
ORIGIN      
        1 aggacaaaca tcgatcaggg tcaaattgac aataaaaggg ctggagcaag caacagggac
       61 tcaccaacca ggcctcgcct ctgagttcag cccagagcta gctgggaaat gttccggatg
      121 ttggccaagg ccagtgtgac gctgggctcc agggcagcaa gttgggtacg gaacatgggc
      181 tcgcaccagc tgctggtgcc acccccagag gccctgagca agcccctgtc aattcctaag
      241 aggctcctgt tgggtccggg accctccaac ctggctcctc gtgtgctagc agctggaagt
      301 ctgaggatga ttggccacat gcaaaaagag atgtttcaga tcatggatga gatcaagcag
      361 ggcatccagt atgtgttcca gaccaggaac cccctcacac tggttgtcag cggctcagga
      421 cattgtgcca tggagactgc cctgttcaac ctcctggagc ctggggactc ctttcttgtg
      481 ggaaccaatg gcatctgggg gatacgggct gcagagatcg ctgagcggat tggagcccgt
      541 gtgcaccaga tgatcaagaa gcctggagaa cattacacac tgcaggaggt ggaggagggc
      601 ctggctcagc ataaaccagt gttgctgttc ctgacccacg gggagtcatc cactggtgtg
      661 ctgcagcccc tggatggttt cggggagctc tgccacaggt atcagtgcct actcctggtg
      721 gactcggtgg catcattggg cggagtccct atctacatgg accaacaagg catcgacatc
      781 ttgtactctg gctctcagaa ggtcctgaat gccccaccag ggatctccct catctccttc
      841 aacgacaagg ccaaatccaa agtctactcc cggaagacaa agccagtctc cttctacaca
      901 gacatcactt atttgtccaa gttgtggggc tgtgagggca agaccagagt aattcatcat
      961 acgttgcctg tcatcagctt atactgcctg agggagagcc tagcactcat ttcagagcag
     1021 ggcctggaga attcctggcg gcgtcacagg gaggctacag cacatctgca caagtgcctg
     1081 cgggagttgg gcttaaagtt ctttgtgaag gacccggaaa tccggctacc tacaatcacc
     1141 accgtgaccg tgcctgccgg ctacaactgg agggacatcg tcagctacgt gctggaccac
     1201 ttcaacattg aaatctctgg tggtcttggg ccctctgagg ataaggtgct gcggattggc
     1261 ctcctgggct acaacgccac cacagagaat gcggaccgtg tagcggaggc cctgagggag
     1321 gccctgcaac attgtcctaa gaataaattg tgagcatcgt ctcaccagac tgtgccctcc
     1381 tggaggggct gggaatatag caggaacgag aagactgtgc aagccctcca gccagcaaag
     1441 gctgccgatg taaccaggcg ggaagggtca gggcgaagct gcccctctcc ccacagatgg
     1501 agccctgtgg tcacatgatg ctaatcacct tccgatgaag ctgcattctg caggccactg
     1561 gacttcggga atattcaata aagtacttgc cagacatcta aaaaaaaaaa aaaaaaaaaa
     1621 aaaaaaaa
//
