LOCUS       NM_001106725             932 bp    mRNA    linear   ROD 26-OCT-2024
DEFINITION  Rattus norvegicus anterior gradient 2, protein disulphide isomerase
            family member (Agr2), transcript variant 1, mRNA.
ACCESSION   NM_001106725 XM_001077035 XM_216691
VERSION     NM_001106725.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Clarke,C., Rudland,P. and Barraclough,R.
  TITLE     The metastasis-inducing protein AGR2 is O-glycosylated upon
            secretion from mammary epithelial cells
  JOURNAL   Mol Cell Biochem 408 (1-2), 245-252 (2015)
   PUBMED   26169982
  REMARK    GeneRIF: Results show that AGR2 is O-glycosylated upon secretion
            from human and rat cell lines, and that this form of the protein is
            released from healthy cells and not as a result of cell lysis.
REFERENCE   2  (bases 1 to 932)
  AUTHORS   Dong,A., Wodziak,D. and Lowe,A.W.
  TITLE     Epidermal growth factor receptor (EGFR) signaling requires a
            specific endoplasmic reticulum thioredoxin for the
            post-translational control of receptor presentation to the cell
            surface
  JOURNAL   J Biol Chem 290 (13), 8016-8027 (2015)
   PUBMED   25666625
REFERENCE   3  (bases 1 to 932)
  AUTHORS   Shi,T., Gao,Y., Quek,S.I., Fillmore,T.L., Nicora,C.D., Su,D.,
            Zhao,R., Kagan,J., Srivastava,S., Rodland,K.D., Liu,T., Smith,R.D.,
            Chan,D.W., Camp,D.G. 2nd, Liu,A.Y. and Qian,W.J.
  TITLE     A highly sensitive targeted mass spectrometric assay for
            quantification of AGR2 protein in human urine and serum
  JOURNAL   J Proteome Res 13 (2), 875-882 (2014)
   PUBMED   24251762
REFERENCE   4  (bases 1 to 932)
  AUTHORS   Patel,P., Clarke,C., Barraclough,D.L., Jowitt,T.A., Rudland,P.S.,
            Barraclough,R. and Lian,L.Y.
  TITLE     Metastasis-promoting anterior gradient 2 protein has a dimeric
            thioredoxin fold structure and a role in cell adhesion
  JOURNAL   J Mol Biol 425 (5), 929-943 (2013)
   PUBMED   23274113
REFERENCE   5  (bases 1 to 932)
  AUTHORS   Ryu,J., Park,S.G., Lee,P.Y., Cho,S., Lee,D.H., Kim,G.H., Kim,J.H.
            and Park,B.C.
  TITLE     Dimerization of pro-oncogenic protein Anterior Gradient 2 is
            required for the interaction with BiP/GRP78
  JOURNAL   Biochem Biophys Res Commun 430 (2), 610-615 (2013)
   PUBMED   23220234
REFERENCE   6  (bases 1 to 932)
  AUTHORS   Riener,M.O., Pilarsky,C., Gerhardt,J., Grutzmann,R.,
            Fritzsche,F.R., Bahra,M., Weichert,W. and Kristiansen,G.
  TITLE     Prognostic significance of AGR2 in pancreatic ductal adenocarcinoma
  JOURNAL   Histol Histopathol 24 (9), 1121-1128 (2009)
   PUBMED   19609859
REFERENCE   7  (bases 1 to 932)
  AUTHORS   Park,S.W., Zhen,G., Verhaeghe,C., Nakagami,Y., Nguyenvu,L.T.,
            Barczak,A.J., Killeen,N. and Erle,D.J.
  TITLE     The protein disulfide isomerase AGR2 is essential for production of
            intestinal mucus
  JOURNAL   Proc Natl Acad Sci U S A 106 (17), 6950-6955 (2009)
   PUBMED   19359471
REFERENCE   8  (bases 1 to 932)
  AUTHORS   Sitek,B., Sipos,B., Alkatout,I., Poschmann,G., Stephan,C.,
            Schulenborg,T., Marcus,K., Luttges,J., Dittert,D.D., Baretton,G.,
            Schmiegel,W., Hahn,S.A., Kloppel,G., Meyer,H.E. and Stuhler,K.
  TITLE     Analysis of the pancreatic tumor progression by a quantitative
            proteomic approach and immunhistochemical validation
  JOURNAL   J Proteome Res 8 (4), 1647-1656 (2009)
   PUBMED   19714807
REFERENCE   9  (bases 1 to 932)
  AUTHORS   Innes,H.E., Liu,D., Barraclough,R., Davies,M.P., O'Neill,P.A.,
            Platt-Higgins,A., de Silva Rudland,S., Sibson,D.R. and Rudland,P.S.
  TITLE     Significance of the metastasis-inducing protein AGR2 for outcome in
            hormonally treated breast cancer patients
  JOURNAL   Br J Cancer 94 (7), 1057-1065 (2006)
   PUBMED   16598187
REFERENCE   10 (bases 1 to 932)
  AUTHORS   Fletcher,G.C., Patel,S., Tyson,K., Adam,P.J., Schenker,M.,
            Loader,J.A., Daviet,L., Legrain,P., Parekh,R., Harris,A.L. and
            Terrett,J.A.
  TITLE     hAG-2 and hAG-3, human homologues of genes involved in
            differentiation, are associated with oestrogen receptor-positive
            breast tumours and interact with metastasis gene C4.4a and
            dystroglycan
  JOURNAL   Br J Cancer 88 (4), 579-585 (2003)
   PUBMED   12592373
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473947.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_216691.4, XM_001077035.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: CF110592.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN16676809 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q16"
     gene            1..932
                     /gene="Agr2"
                     /note="anterior gradient 2, protein disulphide isomerase
                     family member"
                     /db_xref="GeneID:298961"
                     /db_xref="RGD:1304606"
     exon            1..220
                     /gene="Agr2"
                     /inference="alignment:Splign:2.1.0"
     exon            221..366
                     /gene="Agr2"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..755
                     /gene="Agr2"
                     /note="anterior gradient protein 2 homolog; anterior
                     gradient homolog 2; anterior gradient 2 homolog"
                     /codon_start=1
                     /product="anterior gradient protein 2 homolog precursor"
                     /protein_id="NP_001100195.1"
                     /db_xref="GeneID:298961"
                     /db_xref="RGD:1304606"
                     /translation="MEKFSVSAILLLVAISGTLAKDTTVKSGSKKDPKDSRPKLPQTL
                     SRGWGDQLIWTQTYEEALYKSKTSNRPLMVIHHLDECPHSQALKKVFAENKEIQKLAE
                     QFVLLNLIYETTDKHLSPDGQYVPRIVFVDPSLTVRADITGRYSNRLYAYEPSDTALL
                     HDNMKKALKLLKTEL"
     exon            367..430
                     /gene="Agr2"
                     /inference="alignment:Splign:2.1.0"
     exon            431..483
                     /gene="Agr2"
                     /inference="alignment:Splign:2.1.0"
     exon            484..557
                     /gene="Agr2"
                     /inference="alignment:Splign:2.1.0"
     exon            558..621
                     /gene="Agr2"
                     /inference="alignment:Splign:2.1.0"
     exon            622..705
                     /gene="Agr2"
                     /inference="alignment:Splign:2.1.0"
     exon            706..932
                     /gene="Agr2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctttaaggta caagagttct ctccagagtt ccagggccag tggcatcaca gatgactagc
       61 ctggaagggt ccttccacat tcctttgtaa caaggcactc ttcctactga atgtacgggc
      121 atcctcacca aacacaactc tgaataaggt ctagctgagg tgctgagact ggaaagacag
      181 cagctgttgg aagcagcatc ttacttctct tccttttgcg atttgccatg gagaaatttt
      241 cagtctcggc aatcctgctt cttgtggcca tctctggtac tctggccaaa gacaccacag
      301 tcaaatctgg atccaaaaag gacccaaagg actctcgacc caaactaccc cagaccctgt
      361 ccagaggttg gggagatcag ctcatctgga ctcagactta cgaagaagcc ttatacaaat
      421 ccaagacaag caacagaccc ttgatggtca ttcatcactt ggacgaatgc ccgcacagtc
      481 aagctttaaa gaaagtgttt gctgaaaata aggagatcca gaaattggca gagcagtttg
      541 ttctcctcaa cttgatctat gaaacaactg acaagcacct ttctcctgat ggccagtacg
      601 tccccagaat tgtgtttgtg gacccttccc tgacggtgag ggcagacatc accggaagat
      661 actcaaaccg tctctacgct tacgaacctt ctgacacagc tctgctgcac gacaacatga
      721 agaaagctct caagttgctg aagacagagt tgtagagtca actgtacagt gcctcaggag
      781 ccgggaaggc agaagcactg tggacctgcc gatgacatta cagtttaatg ttacaacaaa
      841 tgtatttttt aaacacccac gtgtggggaa acaatattat tatctactac agacacatga
      901 ttttctagaa aataaagtct tgtgagaact cc
//
