LOCUS       NM_001394371            6622 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus argonaute RISC component 4 (Ago4), mRNA.
ACCESSION   NM_001394371 XM_006238862
VERSION     NM_001394371.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Gu S, Jin L, Huang Y, Zhang F and Kay MA.
  TITLE     Slicing-independent RISC activation requires the argonaute PAZ
            domain
  JOURNAL   Curr Biol 22 (16), 1536-1542 (2012)
   PUBMED   22795694
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Modzelewski AJ, Holmes RJ, Hilz S, Grimson A and Cohen PE.
  TITLE     AGO4 regulates entry into meiosis and influences silencing of sex
            chromosomes in the male mouse germline
  JOURNAL   Dev Cell 23 (2), 251-264 (2012)
   PUBMED   22863743
REFERENCE   3  (bases 1 to 6622)
  AUTHORS   Ender C and Meister G.
  TITLE     Argonaute proteins at a glance
  JOURNAL   J Cell Sci 123 (Pt 11), 1819-1823 (2010)
   PUBMED   20484662
  REMARK    Review article
REFERENCE   4  (bases 1 to 6622)
  AUTHORS   Yoda M, Kawamata T, Paroo Z, Ye X, Iwasaki S, Liu Q and Tomari Y.
  TITLE     ATP-dependent human RISC assembly pathways
  JOURNAL   Nat Struct Mol Biol 17 (1), 17-23 (2010)
   PUBMED   19966796
REFERENCE   5  (bases 1 to 6622)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 6622)
  AUTHORS   Rybak A, Fuchs H, Hadian K, Smirnova L, Wulczyn EA, Michel G,
            Nitsch R, Krappmann D and Wulczyn FG.
  TITLE     The let-7 target gene mouse lin-41 is a stem cell specific E3
            ubiquitin ligase for the miRNA pathway protein Ago2
  JOURNAL   Nat Cell Biol 11 (12), 1411-1420 (2009)
   PUBMED   19898466
REFERENCE   7  (bases 1 to 6622)
  AUTHORS   Chi SW, Zang JB, Mele A and Darnell RB.
  TITLE     Argonaute HITS-CLIP decodes microRNA-mRNA interaction maps
  JOURNAL   Nature 460 (7254), 479-486 (2009)
   PUBMED   19536157
REFERENCE   8  (bases 1 to 6622)
  AUTHORS   Wu L, Fan J and Belasco JG.
  TITLE     Importance of translation and nonnucleolytic ago proteins for
            on-target RNA interference
  JOURNAL   Curr Biol 18 (17), 1327-1332 (2008)
   PUBMED   18771919
REFERENCE   9  (bases 1 to 6622)
  AUTHORS   Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G and
            Tuschl T.
  TITLE     Human Argonaute2 mediates RNA cleavage targeted by miRNAs and
            siRNAs
  JOURNAL   Mol Cell 15 (2), 185-197 (2004)
   PUBMED   15260970
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000162.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001394371.2.
            
            On Aug 31, 2021 this sequence version replaced XM_006238862.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-402               JACYVU010000162.1  53374796-53375197   c
            403-568             JACYVU010000162.1  53365359-53365524   c
            569-689             JACYVU010000162.1  53362141-53362261   c
            690-871             JACYVU010000162.1  53358642-53358823   c
            872-1008            JACYVU010000162.1  53358341-53358477   c
            1009-1143           JACYVU010000162.1  53358109-53358243   c
            1144-1231           JACYVU010000162.1  53357135-53357222   c
            1232-1379           JACYVU010000162.1  53352859-53353006   c
            1380-1499           JACYVU010000162.1  53352245-53352364   c
            1500-1628           JACYVU010000162.1  53351976-53352104   c
            1629-1762           JACYVU010000162.1  53351653-53351786   c
            1763-1947           JACYVU010000162.1  53350270-53350454   c
            1948-2107           JACYVU010000162.1  53348631-53348790   c
            2108-2423           JACYVU010000162.1  53344761-53345076   c
            2424-2558           JACYVU010000162.1  53344491-53344625   c
            2559-2660           JACYVU010000162.1  53335965-53336066   c
            2661-2860           JACYVU010000162.1  53335507-53335706   c
            2861-6622           JACYVU010000162.1  53329745-53333506   c
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..6622
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="argonaute RISC component 4"
                     /db_xref="GeneID:298533"
                     /db_xref="RGD:1304583"
     exon            1..402
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     CDS             384..2969
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /note="eukaryotic translation initiation factor 2C, 4;
                     argonaute 4, RISC catalytic component; argonaute RISC
                     catalytic component 4; protein argonaute-4-like"
                     /codon_start=1
                     /product="protein argonaute-4"
                     /protein_id="NP_001381300.1"
                     /db_xref="GeneID:298533"
                     /db_xref="RGD:1304583"
                     /translation="MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDV
                     YHYDVDIKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGRDR
                     VDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLSEVPDDSVQALDVITRHLP
                     SMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYRAQ
                     PIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQMKRKYRVCNVTR
                     RPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYSHLPCLQVGQEQKHTYLPLEVC
                     NIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSVLGGPDPYLKEFGIV
                     VHNEMTELMGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAP
                     QKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQ
                     LIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINAKLGGI
                     NNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQ
                     TSRQEIAQELLFSQEVVQDLTSMARELLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVA
                     WPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDS
                     TITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRS
                     VSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTQH
                     TMYFA"
     exon            403..568
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            569..689
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            690..871
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1008
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1143
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1231
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1379
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1499
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1628
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1762
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1763..1947
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2107
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2423
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2424..2558
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2660
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2860
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
     exon            2861..6622
                     /gene="Ago4"
                     /gene_synonym="Eif2c4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 accccgcccc ctgcctcgga cctgctcgct ggaccctgcg cgtgccgggc tgcgcacgcc
       61 cccttcgcca gccgggaccc cggaccgcac gcgcctggcg ccccctccct gcccggcggg
      121 cgcgcgccgc ctcccgctcc cgctccagct ggcggcggcg gcggcggccc ggattgtttt
      181 tgttgttgcc gaggccggaa gagccgtgag agagccgggt ccctgtcccc ggaccgggca
      241 ctgccgccgc cccttgccca gcgctcgcgt cttcgcggcg ccgcttcagc gccaatattc
      301 cggagagcaa gcgttacgcg gcggcggcgg ggcccggagc gggaggcgcc ggggaccggg
      361 gcgaggcggc ccccgccgcc gccatggagg cgctgggacc cggacctcca gccagcctct
      421 tccagccacc ccggcgtcct ggccttggca ctgttgggaa accaatccga ctgttagcca
      481 atcattttca ggttcagatt cctaaaatag atgtgtatca ctatgatgtg gatattaaac
      541 cggaaaaacg gcctcgtaga gtcaacaggg aggtagtaga cactatggtg cgacacttca
      601 agatgcagat atttggagat cggcagcctg gctatgatgg caaaagaaac atgtacacag
      661 cacatccact gccaatcgga cgggataggg ttgatatgga ggtgaccctc ccaggtgagg
      721 gcaaggacca aacgttcaaa gtgtcagtgc aatgggtgtc agttgtgagc cttcagctgc
      781 ttttagaagc tttagctgga cacctgagtg aagtcccaga tgactcagta caagcccttg
      841 acgtgattac aaggcatctc ccctctatga ggtacactcc agtaggccgg tcttttttct
      901 cacctcctga aggttactac caccctctgg gagggggcag agaggtctgg ttcggctttc
      961 accagtctgt gcgccctgcc atgtggaaga tgatgctcaa cattgatgta tctgcaactg
     1021 ctttctaccg ggctcagccc atcattgagt tcatgtgtga ggttttagac atccagaaca
     1081 tcaacgagca gacgaaacct ctaacagact cccagcgtgt caagtttacc aaagaaatca
     1141 ggggtcttaa agttgaggtg actcactgtg gacagatgaa acgaaaatat cgagtttgta
     1201 atgtgacaag acgaccagcc agtcatcaaa ccttcccttt gcagctagaa aatggtcaag
     1261 ctatggaatg tacagtagct cagtatttta agcaaaaata taatctgcag ctgaaatact
     1321 cccatcttcc ctgccttcaa gtgggacaag agcaaaagca tacatacctg ccacttgagg
     1381 tctgtaatat agtagcaggg cagagatgta taaagaagct cacagacaac cagacatcca
     1441 ccatgatcaa agccacagcg agatctgctc ctgacagaca ggaggaaatc agtagactgg
     1501 tgaagagcaa cagcgtgctg ggtggacctg acccatacct gaaggaattt gggatcgttg
     1561 tgcacaatga gatgacggaa ctcatgggca gggtgcttcc ggcgccaatg ctgcagtatg
     1621 gaggccggaa taaaacagta gccacaccca accagggtgt ctgggacatg cgaggaaagc
     1681 agttttatgc tggcattgaa attaaagtat gggcagtggc ttgttttgcg cctcagaaac
     1741 aatgtaggga agatttgcta aagagcttca ctgaccaact tcgtaaaatc tccaaggatg
     1801 cagggatgcc catccagggt caaccatgtt tctgcaaata tgctcaaggt gcagacagtg
     1861 tggagcccat gtttaaacat ctgaaaatga catatgtggg cctgcagcta atagtggtga
     1921 ttttgcctgg gaagacacca gtatatgcgg aggtgaagcg tgttggagat accctcctag
     1981 gtatggccac gcagtgtgtc caggtcaaaa atgttgtgaa gacctcgcca caaacccttt
     2041 ccaacctttg tctgaagata aacgcaaagc ttggaggaat taacaacgta cttgtgcctc
     2101 atcaaaggcc ctcagtgttc cagcagcctg tcatcttcct gggagcagat gtcactcatc
     2161 cacctgctgg ggatgggaag aagccttcta ttgcagcggt ggtgggcagc atggatggcc
     2221 atcccagtcg gtactgtgcc actgtccggg tgcagacatc tcgccaggag attgcccagg
     2281 agctcctgtt cagtcaggag gtcgtgcagg acctgacgag catggctcgg gagctgctga
     2341 ttcagttcta taagtccacg cgcttcaagc ccacgcgtat catctactac cgcggagggg
     2401 tatccgaggg acagatgaaa caggtagctt ggccagaact aatagcaatt cgaaaggcat
     2461 gtataagtct ggaggaagac taccggccag gaataaccta cattgtggta caaaagcgac
     2521 atcacacacg actcttctgt gcagataaaa cagaaagggt ggggaaaagt ggcaatgtcc
     2581 cagcaggcac tacggtggat agcaccatca cacatccatc ggagtttgac ttttacctct
     2641 gcagtcatgc aggaattcag ggaaccagtc gtccttcaca ttaccaggtc ctgtgggatg
     2701 acaactgctt taccgcggat gaactgcagc tgctgaccta ccagctctgc cacacctacg
     2761 tgaggtgcac acgctcagtc tccattcctg cccctgcata ttatgcccgg cttgtagcat
     2821 tccgggcaag gtatcatttg gtggacaaag atcatgacag tgcggaaggc agtcacgtgt
     2881 caggacagag caacggccgg gatcctcaag ccttggctaa ggcggtgcag atccaccatg
     2941 atacccagca cactatgtat tttgcctgag agtcttggaa aagaactcaa ccaatttggc
     3001 atcccacgca tcctcaaaat gtttcagatg cctactgcct ctcttagtgc acagtgattt
     3061 caggtggtcc ctaccagcag ctcagaaaag ttgcactgaa tctgtactct gtagccctgt
     3121 ctgatgcatc aacaaaatcg agcctttttt tttttttaag aaatagatac tcatagatta
     3181 tgttttctga tgcactgggc agaattttta cctcaacgtg cgaaggctga ttagccttga
     3241 ctttctaaag gactttccaa gaagggtttc tgtggaatat tttgctagct gtggtattca
     3301 ccgcatctag tcttataaga ggagtatttc cttttttctg tgttattgac tggagtgtgt
     3361 acataagaac atttggaaga gagaaaccaa acgacctgga tcatttccat gtaacttttt
     3421 aattgagtgg gtccgtagac ttcttaatgg agatttctta ctttgccact gcaaatgcct
     3481 ttgaggagca ttaagttttg aaagttttcc agaactctgt taccttcact gcgttatctg
     3541 actgacctct ccgggcctgc gctgtagagt acaggctgcc tggtgattgc actaatactt
     3601 ggctgagatt tgaacaggca gctcccttcc ttggctggct tttgtgaatc tgtgacataa
     3661 gcagatgtga tgtgtgtcac agggacagat aacactgcca atggtaaaaa gtacccatgt
     3721 tctccatatt tgggaaaaaa aagaattagt ttttagtatt tttccacatt ttcaaaagtg
     3781 cccattttat gctgctgtgt ggtgtgttag atctaaacga tctttaaact tctctcatag
     3841 aaaattaact ttggcaataa gcatttttaa ggtctctgct ctttttcctc ctgacatagt
     3901 tttctagata agatttcagt atgattttat caactatttc ttgtatctca taactgggat
     3961 attctgaaac cagtttataa agacatggat ttccatgtcc tttaaataat tgttttttta
     4021 aaaaacaaaa caaacctatt ttatagtcag tgtcctgtca ctttaaaaaa aaaaaaaagc
     4081 tggaaccagg atgctttctg attggtcagg ctttgctgac gtataaggct cattgagcag
     4141 tactgcactt gaaaagaaag taccagccag taaaaaaata gtttccctgg gaatcaaggc
     4201 tgcacacaca gctgcttaca agacagcctg aagtgtgctt ggctttgccc atctctctct
     4261 caactaaaac tctgtagaag ttgatttttt tttctctctc tctgtaatag attctggatt
     4321 ccctcacagg aagacaggac ttcttttctt gtcgtaacaa agggagttga tgtaaagcaa
     4381 gtatcagtta gaatgaaggg aaaatgtatc ctgtatttag tgtagatagt acttggtaaa
     4441 ggacaagcgt tacatagtca aggtagtcct acctgcggtt tccagcgcac agttactcca
     4501 acctggaagg gtaaggagtg aaaggcaaga ggctctatgt agagccacac tcctcatggc
     4561 tgaggagggt gctgggaaga gacagacgca atatgctaga gtcaattttg cagtctctct
     4621 gtagaaaccg ttggtgggaa acactgggtc agatgataaa gggtgtttgc ttgaaccctt
     4681 tagttggtcc aaggtccaaa tctgttaaac gggccctctc aatcccagca atataaaaca
     4741 ctgtttccct tcagtctttt aaacaagtgt gtagatacat taaatgttta catttccgtg
     4801 tgctgtcgtc gctcagcggt gccaccagtg tttgctgaga ctggtctcca agagagtttc
     4861 caatgtccat ctaatcctat agcacgtgag ggtgggtgca gaccccctta atctgcagac
     4921 cgtgggaaaa agcaagtcct gaggctgaag cacaacatag cagtgaagag acagcagacg
     4981 tctgttctct gagattttcc ttttctgcaa atacctcaca tggattatac aaatcaggac
     5041 atgttgatga atggctgact gccactttta ttcacacctg ttcagggaaa gcacagaaaa
     5101 tactctgcag cccagccact ctggacccaa gaagagggaa aaaacaaaag gaagcttttt
     5161 cagaaaaagg aaaacttgcc tggagtctgt gaggcgaaga accaagaggg ggctggcagc
     5221 tagccagagg gcaccgtacc tgcactggag tgcctagggc tcgcttgtta acaatacaca
     5281 gaagaaactc ttgggatctt ccgacataat ggaacagtga gcaagccgtg tgcccagacg
     5341 ctccatccac cttcccgcag ttggcttcga ggtttgccca tgaacaatgt atctgaatat
     5401 accgtcagcc ctcgtatgca aggccctggg ttcggtcccc agctccggaa aaaaagaacc
     5461 aaaaaaaaaa aaaaaaaaaa aaaaaaaaca acagaattgc agtcatacca tcgcaagcca
     5521 atctgaatgc atagttttaa ttgaacaaga gttggcaatt caaatagata cccccccccc
     5581 aaaactgact gaattgttat gcatgtgtca atcaatggga ggtgtgcatt ctctagaagt
     5641 agaattaggt tgtttggttt tttaaaacac agcctattta ggtcctgtca tatagcgagg
     5701 aagatttcag tgtagcgact ctaaggagtg actatttggg gctaaggagc acaggacctt
     5761 acggatggga aaggcccaga tgtcgaacca attatgtttt gttgttgttg atgtgttgtc
     5821 gttagttgtt gaaacccgag ctgtttataa agggaagcag ccggcttggt gtagccagtg
     5881 ttctgggcag gtgaaccaca ttcattctcg ctgtaaattc tacgtgctgc ttccaaaggc
     5941 gatggttcac aagcaggtgt ggcctaagtg gtctgtacct taggatctgg tgtctggccc
     6001 atatcctaca tgaaaacctg cccacctggc tcagctaccc actccttccc atggacaaag
     6061 ataaagcact gttttaacca ggctcacccc gtctcccccg ccatggttac cctccctccg
     6121 aggacgctct gagactccct tgcttagtac aaggtggctg ctctgctttc attttgagaa
     6181 agcagaggtt agggcgggcg ccatattaat actgcttcaa catcaaccat tttcttaccc
     6241 cttcctgccg acactcacat tctttctgca tccaccagcc aaaagtgctt gtgttcatct
     6301 gaccgatggt gtggtttctg tttctgtatg cttagacttg taatgcttta aaatgcattt
     6361 tactaatttg gactggatgt ctgtctccag cgatgcgagg tactttctaa gctatgaagg
     6421 aagggtttgt atccttatgt gagatgagta acggttgttt aaattttgca gaggttcttg
     6481 ttactgttac tggtttgggg ttttttggtt tttggggggg gttttgtttg tttgtttgtt
     6541 tgtttgtttt ggcttgcagg gatattttgt gttcatgtgt ctgaaaagtg gggaggggaa
     6601 taaatggcat tcttgtgcta aa
//
