LOCUS       NM_023026               4884 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH
            domain 2 (Agap2), mRNA.
ACCESSION   NM_023026 XM_001054911 XM_576238
VERSION     NM_023026.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Jiang,Y., Liao,L., Shrestha,C., Ji,S., Chen,Y., Peng,J., Wang,L.,
            Liao,E. and Xie,Z.
  TITLE     Reduced expression of E-cadherin and p120-catenin and elevated
            expression of PLC-gamma1 and PIKE are associated with
            aggressiveness of oral squamous cell carcinoma
  JOURNAL   Int J Clin Exp Pathol 8 (8), 9042-9051 (2015)
   PUBMED   26464646
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Chan,C.B., Liu,X., Zhao,L., Liu,G., Lee,C.W., Feng,Y. and Ye,K.
  TITLE     PIKE is essential for oligodendroglia development and CNS
            myelination
  JOURNAL   Proc Natl Acad Sci U S A 111 (5), 1993-1998 (2014)
   PUBMED   24449917
  REMARK    GeneRIF: PIKE plays pivotal roles to advance oligodendroglia
            development and myelinogenesis through Akt/mTOR activation.
REFERENCE   3  (bases 1 to 4884)
  AUTHORS   Dwane,S., Durack,E., O'Connor,R. and Kiely,P.A.
  TITLE     RACK1 promotes neurite outgrowth by scaffolding AGAP2 to FAK
  JOURNAL   Cell Signal 26 (1), 9-18 (2014)
   PUBMED   24056044
  REMARK    GeneRIF: RACK1 scaffolds AGAP2 to FAK to regulate FAK activity and
            cell adhesion during the differentiation process.
REFERENCE   4  (bases 1 to 4884)
  AUTHORS   Chan,C.B., Chen,Y., Liu,X., Papale,L., Escayg,A., Mei,L. and Ye,K.
  TITLE     Essential role of PIKE GTPases in neuronal protection against
            excitotoxic insults
  JOURNAL   Adv Biol Regul 52 (1), 66-76 (2012)
   PUBMED   21925531
REFERENCE   5  (bases 1 to 4884)
  AUTHORS   Chan,C.B., Chen,Y., Liu,X., Tang,X., Lee,C.W., Mei,L. and Ye,K.
  TITLE     PIKE-mediated PI3-kinase activity is required for AMPA receptor
            surface expression
  JOURNAL   EMBO J 30 (20), 4274-4286 (2011)
   PUBMED   21847098
  REMARK    GeneRIF: PIKE is a critical factor in controlling synaptic AMPA
            receptor insertion.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4884)
  AUTHORS   Xia,C., Ma,W., Stafford,L.J., Liu,C., Gong,L., Martin,J.F. and
            Liu,M.
  TITLE     GGAPs, a new family of bifunctional GTP-binding and
            GTPase-activating proteins
  JOURNAL   Mol Cell Biol 23 (7), 2476-2488 (2003)
   PUBMED   12640130
REFERENCE   7  (bases 1 to 4884)
  AUTHORS   Nie,Z., Stanley,K.T., Stauffer,S., Jacques,K.M., Hirsch,D.S.,
            Takei,J. and Randazzo,P.A.
  TITLE     AGAP1, an endosome-associated, phosphoinositide-dependent
            ADP-ribosylation factor GTPase-activating protein that affects
            actin cytoskeleton
  JOURNAL   J Biol Chem 277 (50), 48965-48975 (2002)
   PUBMED   12388557
REFERENCE   8  (bases 1 to 4884)
  AUTHORS   Ye,K., Aghdasi,B., Luo,H.R., Moriarity,J.L., Wu,F.Y., Hong,J.J.,
            Hurt,K.J., Bae,S.S., Suh,P.G. and Snyder,S.H.
  TITLE     Phospholipase C gamma 1 is a physiological guanine nucleotide
            exchange factor for the nuclear GTPase PIKE
  JOURNAL   Nature 415 (6871), 541-544 (2002)
   PUBMED   11823862
REFERENCE   9  (bases 1 to 4884)
  AUTHORS   Ye,K., Hurt,K.J., Wu,F.Y., Fang,M., Luo,H.R., Hong,J.J.,
            Blackshaw,S., Ferris,C.D. and Snyder,S.H.
  TITLE     Pike. A nuclear gtpase that enhances PI3kinase activity and is
            regulated by protein 4.1N
  JOURNAL   Cell 103 (6), 919-930 (2000)
   PUBMED   11136977
REFERENCE   10 (bases 1 to 4884)
  AUTHORS   Yamane,O., Hayashi,T., Sako,S., Kihara,T. and Koyama,M.
  TITLE     Studies on hemorrhagic diathesis of experimental bovine bracken
            poisoning. I. Detection of circulating anticoagulants
  JOURNAL   Nihon Juigaku Zasshi 37 (4), 335-340 (1975)
   PUBMED   1238857
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF280816.1, AY128688.1 and JAXUCZ010000007.1.
            
            On or before Sep 25, 2009 this sequence version replaced
            XM_001054911.1, XM_576238.2, NM_023026.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY128688.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2254              AF280816.1         1-2254
            2255-2839           AY128688.1         2255-2839
            2840-3062           JAXUCZ010000007.1  64797365-64797587
            3063-3318           JAXUCZ010000007.1  64797683-64797938
            3319-4884           JAXUCZ010000007.1  64798059-64799624
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q22"
     gene            1..4884
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /note="ArfGAP with GTPase domain, ankyrin repeat and PH
                     domain 2"
                     /db_xref="GeneID:65218"
                     /db_xref="RGD:628844"
     CDS             1..3561
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /note="centaurin, gamma 1; AGAP-2; cnt-g1; nuclear GTPase
                     PIKE; phosphatidylinositol 3-kinase enhancer"
                     /codon_start=1
                     /product="arf-GAP with GTPase, ANK repeat and PH
                     domain-containing protein 2"
                     /protein_id="NP_075415.2"
                     /db_xref="GeneID:65218"
                     /db_xref="RGD:628844"
                     /translation="MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAVGAPGA
                     RGSEPRDPGSPRGAEEPGKKRHERLFHRQDALWISTSSAGAGGAEPPALSPAPASPAR
                     PVSPAPGRRLSLWAAPPGPPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTG
                     GGVPGSSSPHPGTGSRRLKVAPPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGK
                     PRVKGSKSTAGTGASAVAAGGGGSAAVTTSGGVGAGAGARGKLSPRKGKSKTLDNSDL
                     HPGPSAGSPPLTVPAIPVPATSVTAASTQPLGPAPPITLEPPAPGLKRGREGGRASTR
                     DRKMLKFISGIFTKSTGGPPGPGPLPGPQGLSSSSGSRELLGAELRASPKAVVNSQEW
                     TLSRSIPELRLGVLGDVRSGKSSLIHRFLTGSYQVLEKPESEQYKKEMLVDGQTHLVL
                     IREEAGAPDAKFSGWADAVIFVFSLEDESSFQAVSHLHGQLISLRGEGRGGLALALVG
                     TQDRISASSPRVVGDARARALCTDMKRCSYYETCATYGLNVDRVFQEVAQKVVTLRKQ
                     QQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAV
                     AGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSG
                     NSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISA
                     FGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQLPTDQTSKHLLKPDRNLA
                     RALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEGSAVQAEAKRKMWKLKS
                     FGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSK
                     VKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALICIECSGIHRNLGT
                     HLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSSREERESWIRA
                     KYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSVEDPQLRSP
                     LHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQHGCPGEG
                     GSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV"
     exon            1..1150
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1209
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1297
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1383
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1531
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1666
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1776
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1935
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2022
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2127
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2290
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            2291..2410
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            2411..2539
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            2540..2599
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2748
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2839
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..3062
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            3063..3318
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
     exon            3319..4884
                     /gene="Agap2"
                     /gene_synonym="Centg1; Pike"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgagccggg gcgcgggcgc gcttcagcgc cggacaacga cctacctcat ctcgctgacc
       61 ctggtcaagc tcgagtcggt acctccgccg ccgccttctc cgtctgcagc tgcggtcggc
      121 gcccctgggg ccagaggctc ggagccccga gatcctggca gcccccgagg cgcggaggaa
      181 cccggcaaga agcggcacga gcgtctcttc caccggcagg atgcgctgtg gatcagtacc
      241 agcagcgcgg gcgccggggg cgcggagccc cccgcgctgt ccccggctcc ggccagtccg
      301 gctcgcccgg tctccccggc tccgggccgc cgactgtccc tctgggccgc ccctccggga
      361 cccccgctct ccggggggct gagcccggac tccaagccgg ggggcgcccc ctcttcctct
      421 cggcgccctc tactcagcag cccgagctgg gggggtccgg aacctgaagg ccggacgggt
      481 ggcggcgtcc cgggctcgtc ctccccgcat cctggcaccg gtagccgaag gctcaaggtg
      541 gcgcctcctc cgccagctcc caagcctttc aagaccgtga ccactagtgg agccaaagcc
      601 ggcgggggca agggcgcggg tagccgcctg tcatggcccg aaagcgaggg caaacccagg
      661 gtcaaggggt caaagagcac cgccgggact ggagcctctg ccgttgcggc cggcggggga
      721 gggagcgctg cagtcacgac ctctggtggg gtcggtgctg gggctggagc gagaggaaaa
      781 ttgtcccctc ggaaaggcaa gagtaagacc ttggacaata gtgacttgca cccaggaccc
      841 agtgccggtt ctcctccgct caccgtgcca gcaatcccag ttccagctac ttctgtcact
      901 gccgcctcca cgcagcccct cgggcctgca ccccctatca ctctggagcc accagctccg
      961 gggctaaaaa ggggccggga gggcggccga gcgtccactc gtgaccgcaa gatgctcaag
     1021 tttatcagcg gtatcttcac caagagcaca ggagggcctc ccggtcccgg gccccttccc
     1081 ggaccccaag gcttgtcttc cagcagcggg tccagggagc tgctgggcgc agagctacgc
     1141 gcctccccta aggctgtggt caatagtcag gaatggactt tgagccgatc aattcccgaa
     1201 ctgcgcctgg gtgtgctggg tgatgtcagg agtgggaagt catccctcat ccacagattc
     1261 ctcacgggtt cataccaggt gctggagaag cctgagagtg agcagtacaa gaaggagatg
     1321 ctggtggatg ggcagaccca tctggtgctg atccgagagg aagcaggggc accggatgcc
     1381 aagttctcag gctgggcaga tgctgtgatc ttcgtcttca gcctggagga tgagagcagc
     1441 ttccaggctg tgagccatct ccatgggcag ctgatctccc tccgggggga gggacgagga
     1501 ggtctggccc ttgccctggt tgggacccaa gacaggatca gcgcatcgtc ccctcgagtg
     1561 gtgggtgatg ctcgagcccg ggcattgtgt accgacatga agcgctgcag ctactatgag
     1621 acctgtgcaa cctatgggct caatgtggac cgggtcttcc aggaggtggc ccagaaggtg
     1681 gtgaccttgc gcaaacagca gcaactcctg gcggcttgca agtccctgcc cagctcaccg
     1741 agccattcag ctgcttctac tcctgtagct gggcaggcta gtaatggggg ccacactagc
     1801 gactactctt cttccctccc atcttcaccc aatgttggac accgggagct ccgggctgag
     1861 gcagctgcgg tggctgggtt gagcacccca gggtccttac atcgagcagc caagcggcgg
     1921 accagccttt ttgcgaaccg tcgaggcagt gattctgaga aacggagctt agacagtcgg
     1981 ggcgagacaa cagggagtgg gcgagccatc cccattaaac agagtttcct gctaaagcga
     2041 agtggcaatt ccttgaacaa agagtggaag aagaaatacg tgaccttgtc cagcaatggc
     2101 tttctgctct accaccctag cattaatgat tacatccaca gtacacacgg caaagagatg
     2161 gacttgttac gaacaacagt caaagtccca ggcaagcggc ccccaagggc tatctctgct
     2221 ttcggcccct cagccagcat caatgggttg gtcaaggata tgagcactgt ccagatgggt
     2281 gaaggccctg aagcctcaac tccaatgcca agccccagtc ccagccccag ctccctgcag
     2341 ctcccaacag accaaacatc caagcacctg ttgaagccag accggaattt ggcccgtgcc
     2401 cttagcaccg actgtacccc atctggagac ctgagtcccc tgagtcggga acccccacct
     2461 tctcccatgg tgaagaagca gaggaggaag aaattgtcca caccatctaa gactgaaggc
     2521 tcagctgtgc aggctgaagc caagcgcaaa atgtggaaac taaaatcctt tggtagttta
     2581 agaaatattt ataaagcaga ggaaaacttt gagttcctga tcgtgtccag cactggtcag
     2641 acgtggcact ttgaggcagc cagcttcgag gagagggatg cctgggttca ggccattgag
     2701 agtcagatcc tggccagtct gcaatgctgt gagagcagca aggtcaagct gcgcactgac
     2761 agccaaagtg aagccgtggc catccaggcg atccgcaacg caaagggaaa ctcgacctgt
     2821 gtggactgtg gagcccccaa ccccacgtgg gccagtttga atctgggcgc actcatctgc
     2881 atcgaatgtt ctggcatcca ccggaacctg ggcacgcacc tgtctcgagt gcgttcgctg
     2941 gacttggatg actggccgcg ggagctgacc ctggtgctga cggccattgg caacgacacg
     3001 gccaatcgcg tgtgggagag cgacactcgg ggccgtgcca agcccacgcg ggactcttcg
     3061 agggaggagc gtgagtcgtg gattcgggcc aagtacgaac agctgctgtt cctggcgcct
     3121 ctgggcacca cggaggagcc gctgggccgc caactgtggg ccgctgtgga ggcccaggac
     3181 gtggccgccg ttctgctgct tctggcccac gcgcgacatg ggccgctgga caccagcgtg
     3241 gaggaccctc agctccgctc gccgctgcac ttggcggccg agctcgccca cgttgttatt
     3301 acacagctgc tgttgtggta tggcgcagac gtggcggccc gcgatgcgca gggccgcaca
     3361 gcattgttct acgcccgcca ggctggcagc cagctatgtg cagacatcct cctccagcac
     3421 ggctgtccgg gagagggcgg cagtacagcc accacgccca gcgcggccac cacccccagc
     3481 attaccgcca cgcccagccc gaggcgccgg agcagtgctg ccagcttggg tcgcgtggac
     3541 accacgatcg cgctggtata gttgcccagc cgcgggagag acacccctca cccccgcgcg
     3601 ggccaggcac gactacaccg ggtggaccat tggacagatg cacccactca cctctccagt
     3661 ccgcaccccg tcccacggga gcactttcta cccccacgag ggcacagccc cgcacgcctc
     3721 ccaggagaca caaactcacc tccctctccc cagcgaggca tggagaccct agctcatcac
     3781 gcccctactt cacagcttcc ttacttggat tgccctgggt ctacctgctg ggtgtttgta
     3841 ggtgggcact ccctgtatgg gctccaaggt gcttggactt gctggcttgt gccccctagg
     3901 ggcggggaaa agatccagtc ctgcctgcgt tctacaatcg ggtggccgga gccgaaaccc
     3961 aggctggctc ctggggcaac gccacaccag agggaggggt tagtatgtgg aagactagcc
     4021 ctgggacttg ggaccaattt ggggacccca ccccttccat gctaatctca ctgcccagtg
     4081 aggggggagg gcagcgaggg gcaggacccc tgctgcccat ggaggtggag gggtccccaa
     4141 ccctttccgt gaaagggacc atgaggagtg gaaatcagat atcccccctc tacctatcta
     4201 tgaatggaag ctaaagagcc agggggagcc gagagcctgg gtccttcccc ttacaatctt
     4261 ggggtagggg aaggaggatt ggaacaggag gggactagag aggggccttg taatttattg
     4321 ctttgttccc caacatgggg atggggtggg taggggcaag gggagggcat ttcagggtag
     4381 gggaagccag gggtgggtag gggtggggtg ggatgggggt gctctcgggt tgtgtctgtg
     4441 actgtgactg cgtacctgtg actgtgcagc gcccgtggtg atctggggta gggggcaccc
     4501 ctacaatggg acccctcccc cataatcctt tctgtccagc ccctctctca ctggagcagc
     4561 tccaggtccg tttctccttt gctccctccc agccggggct gagagggtgt gtagtggctg
     4621 gaagggtttc aaggtccttg ccccatcccc agaaaggggg agatggtgtg gacttctctg
     4681 gcccagccct gccaccctta ttcagcccgg ctttgggccg tctgtgtcag tggtttccgg
     4741 tctttttttt tcctggctaa aatattttgc aaaagaccag gtaattgggc agtggagtga
     4801 agagggggca ggggttaccc ccctcacctc caaagcaggt ggtgtgaatc ttaacagcaa
     4861 ttaaagagca agtggttttg tcta
//
