LOCUS       NM_172320               2025 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus afamin (Afm), mRNA.
ACCESSION   NM_172320 XM_008770001
VERSION     NM_172320.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Mihara,E., Hirai,H., Yamamoto,H., Tamura-Kawakami,K., Matano,M.,
            Kikuchi,A., Sato,T. and Takagi,J.
  TITLE     Active and water-soluble form of lipidated Wnt protein is
            maintained by a serum glycoprotein afamin/alpha-albumin
  JOURNAL   Elife 5, e11621 (2016)
   PUBMED   26902720
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Bastos-Amador,P., Royo,F., Gonzalez,E., Conde-Vancells,J.,
            Palomo-Diez,L., Borras,F.E. and Falcon-Perez,J.M.
  TITLE     Proteomic analysis of microvesicles from plasma of healthy donors
            reveals high individual variability
  JOURNAL   J Proteomics 75 (12), 3574-3584 (2012)
   PUBMED   22516433
REFERENCE   3  (bases 1 to 2025)
  AUTHORS   Kratzer,I., Bernhart,E., Wintersperger,A., Hammer,A., Waltl,S.,
            Malle,E., Sperk,G., Wietzorrek,G., Dieplinger,H. and Sattler,W.
  TITLE     Afamin is synthesized by cerebrovascular endothelial cells and
            mediates alpha-tocopherol transport across an in vitro model of the
            blood-brain barrier
  JOURNAL   J Neurochem 108 (3), 707-718 (2009)
   PUBMED   19046407
REFERENCE   4  (bases 1 to 2025)
  AUTHORS   Palmer,D.J., Kelly,V.C., Smit,A.M., Kuy,S., Knight,C.G. and
            Cooper,G.J.
  TITLE     Human colostrum: identification of minor proteins in the aqueous
            phase by proteomics
  JOURNAL   Proteomics 6 (7), 2208-2216 (2006)
   PUBMED   16502470
REFERENCE   5  (bases 1 to 2025)
  AUTHORS   Jerkovic,L., Voegele,A.F., Chwatal,S., Kronenberg,F.,
            Radcliffe,C.M., Wormald,M.R., Lobentanz,E.M., Ezeh,B., Eller,P.,
            Dejori,N., Dieplinger,B., Lottspeich,F., Sattler,W., Uhr,M.,
            Mechtler,K., Dwek,R.A., Rudd,P.M., Baier,G. and Dieplinger,H.
  TITLE     Afamin is a novel human vitamin E-binding glycoprotein
            characterization and in vitro expression
  JOURNAL   J Proteome Res 4 (3), 889-899 (2005)
   PUBMED   15952736
REFERENCE   6  (bases 1 to 2025)
  AUTHORS   Hinojos,C.A., Boerwinkle,E., Fornage,M. and Doris,P.A.
  TITLE     Combined genealogical, mapping, and expression approaches to
            identify spontaneously hypertensive rat hypertension candidate
            genes
  JOURNAL   Hypertension 45 (4), 698-704 (2005)
   PUBMED   15710778
REFERENCE   7  (bases 1 to 2025)
  AUTHORS   Voegele,A.F., Jerkovic,L., Wellenzohn,B., Eller,P., Kronenberg,F.,
            Liedl,K.R. and Dieplinger,H.
  TITLE     Characterization of the vitamin E-binding properties of human
            plasma afamin
  JOURNAL   Biochemistry 41 (49), 14532-14538 (2002)
   PUBMED   12463752
REFERENCE   8  (bases 1 to 2025)
  AUTHORS   Belanger,L., Roy,S. and Allard,D.
  TITLE     New albumin gene 3' adjacent to the alpha 1-fetoprotein locus
  JOURNAL   J Biol Chem 269 (8), 5481-5484 (1994)
   PUBMED   7509788
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On or before Sep 30, 2022 this sequence version replaced
            XM_008770001.3, NM_172320.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643286.38047.1,
                                           SRR26643287.30053.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760400,
                                           SAMEA5760476 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-129               JAXUCZ010000014.1  17847911-17848039   c
            130-178             JAXUCZ010000014.1  17845406-17845454   c
            179-311             JAXUCZ010000014.1  17845085-17845217   c
            312-523             JAXUCZ010000014.1  17843411-17843622   c
            524-656             JAXUCZ010000014.1  17835627-17835759   c
            657-754             JAXUCZ010000014.1  17834955-17835052   c
            755-884             JAXUCZ010000014.1  17834234-17834363   c
            885-1099            JAXUCZ010000014.1  17827060-17827274   c
            1100-1232           JAXUCZ010000014.1  17823324-17823456   c
            1233-1330           JAXUCZ010000014.1  17821172-17821269   c
            1331-1463           JAXUCZ010000014.1  17820188-17820320   c
            1464-1687           JAXUCZ010000014.1  17818613-17818836   c
            1688-1820           JAXUCZ010000014.1  17817147-17817279   c
            1821-1883           JAXUCZ010000014.1  17816107-17816169   c
            1884-2025           JAXUCZ010000014.1  17815194-17815335   c
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p22"
     gene            1..2025
                     /gene="Afm"
                     /note="afamin"
                     /db_xref="GeneID:282708"
                     /db_xref="RGD:628614"
     exon            1..129
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1868
                     /gene="Afm"
                     /note="alpha-Alb; alpha-albumin"
                     /codon_start=1
                     /product="afamin precursor"
                     /protein_id="NP_758823.2"
                     /db_xref="GeneID:282708"
                     /db_xref="RGD:628614"
                     /translation="MRHLKLTGFIFFLLSLTESLALPTKPQDVDHFNATQKFINENVA
                     YLTIIASAQYVQEASFEEVEMLVKVMLDYKDRCLADSTLPECSKIANDAIQDMLCDMK
                     GLPQKHNFSHCCRQAGFQRRLCFFYNKKANVGFLPPFPTLDPEEKCQAYKNNSESFLN
                     LYMYEVARRNPFAFAPVLLNVAARFEEAATTCCEQQQKATCFQDKAAPITQYLKALSS
                     YQRNVCGALLKFGPKTLNSINIAVFSKKFPKIGFEDLTSLLEDVSSMYDGCCEGDVVQ
                     CIRSQSQVMHHICSKQDSISSKIKACCEKKPPERADCIINANKDDRPEDLSLRTPKFT
                     DSENVCQERDSEQDKFFAEFLYDYSRRHTELSTPELLRITKVYKDLLEDCCNRKNPLS
                     CYRHAEDKFNETTERSLAMVQQECKQFQELGKDALQRHFLVKFTKAAPQLPMEELVSL
                     SKEMVAALATCCTLSDEFACVDNLADLVLGELCGINKNRTINPTVDHCCRADFAFRRP
                     CFEHLKADTTYALPSVSALVSALRADWCQPLKEDLQNKRHRFLVNLVKWMPEITDEER
                     LCLFTKFTAAGEECGNIQKPEACFSPESSKTGDVSQDAEKQR"
     exon            130..178
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            179..311
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            312..523
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            524..656
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            657..754
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            755..884
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1099
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1232
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1330
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1463
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1687
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1820
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..1883
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2025
                     /gene="Afm"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acattactct cttgaatgtg gcctcttttt taaatttaaa catgagacac ttaaaactta
       61 caggttttat ttttttcctg ttgtctttga ctgaatccct agccctaccc acaaagcctc
      121 aggatgtaga tcatttcaat gctactcaga aattcataaa tgagaacgtt gcatacctga
      181 ccatcattgc atctgcccag tatgttcagg aagcatcctt tgaggaggtg gaaatgctgg
      241 tgaaggtcat gctggattac aaggataggt gcttggctga cagcacactt ccggagtgtt
      301 caaagatagc taatgatgct atccaggaca tgctctgtga tatgaagggg ctgccacaga
      361 agcataactt ctcccattgc tgtaggcaag ccggctttca aagaagactc tgcttcttct
      421 acaacaagaa agctaatgta ggatttctgc cccctttccc tactctggat ccagaagaga
      481 aatgccaagc ttataaaaat aacagtgaat cttttctcaa cctctatatg tatgaggtgg
      541 ccagaaggaa cccctttgcc ttcgccccag ttcttctgaa cgttgccgct aggtttgagg
      601 aggcggcgac cacgtgctgt gaacagcaac agaaagccac ctgctttcaa gacaaggcag
      661 cccccatcac acagtattta aaagcgttat cttcttatca aagaaatgtt tgtggagccc
      721 ttttaaaatt tggacccaag accttaaact ctataaacat cgccgtgttt agtaagaagt
      781 tccccaagat tggatttgaa gaccttacgt ccctcttaga agatgtttct tccatgtatg
      841 acgggtgctg tgaaggggac gtcgtgcagt gcatccggag ccagagccag gttatgcacc
      901 atatttgttc aaaacaagac tccatctcaa gtaagatcaa agcgtgctgt gagaagaaac
      961 cgccagagcg cgcagactgc ataatcaacg caaacaagga tgacagacca gaagacttat
     1021 ctctgagaac acccaaattt accgacagtg aaaatgtgtg ccaagagcga gattccgagc
     1081 aggacaagtt ctttgctgag tttctttatg attactccag aagacatact gaactgtcta
     1141 caccagagct cctaagaatt actaaagtgt ataaggatct cctggaagat tgctgcaaca
     1201 gaaaaaaccc gttaagctgt taccgccatg cggaggataa gttcaacgag acaacagagc
     1261 gaagccttgc gatggtccag caagagtgca aacagttcca ggaattgggg aaggacgctc
     1321 tacaacgcca tttccttgtc aagttcacca aggcagcccc tcaactcccc atggaagaac
     1381 tggtctccct tagtaaggaa atggtggcgg ctttggcaac ctgctgcact ctcagtgacg
     1441 agttcgcctg tgtggacaac ttggcagatt tagttcttgg agaattatgt ggaataaata
     1501 agaatcgaac tatcaatccc actgtggatc actgctgcag agctgacttt gccttcagga
     1561 gaccctgctt tgagcacctg aaagccgaca ccacctatgc actcccatca gtctctgcac
     1621 ttgtatctgc tttgcgtgca gactggtgtc agcctctcaa ggaggacctt cagaataaga
     1681 gacacaggtt tctggtcaac ttagtgaagt ggatgccaga gatcacagat gaggaacgtc
     1741 tgtgtttgtt cacaaagttc acggctgctg gggaggagtg tggcaacata cagaagcctg
     1801 aggcctgttt cagtccagag agttcaaaaa ccggtgatgt gagtcaagat gcagagaaac
     1861 aaaggtgaac ggaatgacca gagccagtac cagctcacag gatcgtcctg tggagaagtt
     1921 cagctcttaa gaagactgga atagaaggtc cttctgtaac cctttcttga atcatgttcc
     1981 tacaaaaggc aataaaaagg gaggataaag tttctcgccg actaa
//
