LOCUS       NM_013217               7442 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus afadin, adherens junction formation factor
            (Afdn), mRNA.
ACCESSION   NM_013217
VERSION     NM_013217.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Maruo,T., Mizutani,K., Miyata,M., Kuriu,T., Sakakibara,S.,
            Takahashi,H., Kida,D., Maesaka,K., Sugaya,T., Sakane,A., Sasaki,T.,
            Takai,Y. and Mandai,K.
  TITLE     s-Afadin binds to MAGUIN/Cnksr2 and regulates the localization of
            the AMPA receptor and glutamatergic synaptic response in
            hippocampal neurons
  JOURNAL   J Biol Chem 299 (4), 103040 (2023)
   PUBMED   36803960
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Matsuda,T., Namura,A. and Oinuma,I.
  TITLE     Dynamic spatiotemporal patterns of alternative splicing of an
            F-actin scaffold protein, afadin, during murine development
  JOURNAL   Gene 689, 56-68 (2019)
   PUBMED   30572094
  REMARK    GeneRIF: Spatiotemporal and dynamic alternative splicing produces
            differential expression patterns of afadin isoforms and alternative
            splicing of afadin is controlled by the neuron-specific
            regulator(s) whose activity is triggered and dynamically altered
            during neuronal differentiation and maturation.
REFERENCE   3  (bases 1 to 7442)
  AUTHORS   Sellers,K.J., Watson,I.A., Gresz,R.E., Raval,P. and Srivastava,D.P.
  TITLE     Cyto-nuclear shuttling of afadin is required for rapid
            estradiol-mediated modifications of histone H3
  JOURNAL   Neuropharmacology 143, 153-162 (2018)
   PUBMED   30268521
  REMARK    GeneRIF: Study found that afadin undergoes a bi-directional
            trafficking to both synaptic and nuclear compartment in response to
            acute 17beta-estradiol (estradiol) treatment, in mixed sex neuronal
            cultures derived from fetal cortex. Nuclear accumulation of afadin
            was coincidental with an increase in the phosphorylation of histone
            H3 at serine 10 (H3S10p). This epigenetic modification is
            associated with the remodeling of chromatin.
REFERENCE   4  (bases 1 to 7442)
  AUTHORS   Shah,J., Rouaud,F., Guerrera,D., Vasileva,E., Popov,L.M.,
            Kelley,W.L., Rubinstein,E., Carette,J.E., Amieva,M.R. and Citi,S.
  TITLE     A Dock-and-Lock Mechanism Clusters ADAM10 at Cell-Cell Junctions to
            Promote alpha-Toxin Cytotoxicity
  JOURNAL   Cell Rep 25 (8), 2132-2147 (2018)
   PUBMED   30463011
REFERENCE   5  (bases 1 to 7442)
  AUTHORS   Perez White,B.E., Ventrella,R., Kaplan,N., Cable,C.J., Thomas,P.M.
            and Getsios,S.
  TITLE     EphA2 proteomics in human keratinocytes reveals a novel association
            with afadin and epidermal tight junctions
  JOURNAL   J Cell Sci 130 (1), 111-118 (2017)
   PUBMED   27815408
REFERENCE   6  (bases 1 to 7442)
  AUTHORS   Kawabe,H., Hata,Y., Takeuchi,M., Ide,N., Mizoguchi,A. and Takai,Y.
  TITLE     nArgBP2, a novel neural member of ponsin/ArgBP2/vinexin family that
            interacts with synapse-associated protein 90/postsynaptic
            density-95-associated protein (SAPAP)
  JOURNAL   J Biol Chem 274 (43), 30914-30918 (1999)
   PUBMED   10521485
REFERENCE   7  (bases 1 to 7442)
  AUTHORS   Takahashi,K., Nakanishi,H., Miyahara,M., Mandai,K., Satoh,K.,
            Satoh,A., Nishioka,H., Aoki,J., Nomoto,A., Mizoguchi,A. and
            Takai,Y.
  TITLE     Nectin/PRR: an immunoglobulin-like cell adhesion molecule recruited
            to cadherin-based adherens junctions through interaction with
            Afadin, a PDZ domain-containing protein
  JOURNAL   J Cell Biol 145 (3), 539-549 (1999)
   PUBMED   10225955
REFERENCE   8  (bases 1 to 7442)
  AUTHORS   Hock,B., Bohme,B., Karn,T., Yamamoto,T., Kaibuchi,K., Holtrich,U.,
            Holland,S., Pawson,T., Rubsamen-Waigmann,H. and Strebhardt,K.
  TITLE     PDZ-domain-mediated interaction of the Eph-related receptor
            tyrosine kinase EphB3 and the ras-binding protein AF6 depends on
            the kinase activity of the receptor
  JOURNAL   Proc Natl Acad Sci U S A 95 (17), 9779-9784 (1998)
   PUBMED   9707552
REFERENCE   9  (bases 1 to 7442)
  AUTHORS   Mandai,K., Nakanishi,H., Satoh,A., Obaishi,H., Wada,M.,
            Nishioka,H., Itoh,M., Mizoguchi,A., Aoki,T., Fujimoto,T.,
            Matsuda,Y., Tsukita,S. and Takai,Y.
  TITLE     Afadin: A novel actin filament-binding protein with one PDZ domain
            localized at cadherin-based cell-to-cell adherens junction
  JOURNAL   J Cell Biol 139 (2), 517-528 (1997)
   PUBMED   9334353
  REMARK    Erratum:[J Cell Biol 1997 Nov 17;139(4):1060]
REFERENCE   10 (bases 1 to 7442)
  AUTHORS   Nakano,K., Matuda,S., Yamanaka,T., Tsubouchi,H., Nakagawa,S.,
            Titani,K., Ohta,S. and Miyata,T.
  TITLE     Purification and molecular cloning of succinyltransferase of the
            rat alpha-ketoglutarate dehydrogenase complex. Absence of a
            sequence motif of the putative E3 and/or E1 binding site
  JOURNAL   J Biol Chem 266 (28), 19013-19017 (1991)
   PUBMED   1918017
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Feb 26, 2021 this sequence version replaced NM_013217.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U83230.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on computational evidence
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-233               JAXUCZ010000001.1  58041641-58041873
            234-429             JAXUCZ010000001.1  58080269-58080464
            430-542             JAXUCZ010000001.1  58083249-58083361
            543-706             JAXUCZ010000001.1  58084949-58085112
            707-867             JAXUCZ010000001.1  58086633-58086793
            868-1025            JAXUCZ010000001.1  58094948-58095105
            1026-1137           JAXUCZ010000001.1  58099075-58099186
            1138-1305           JAXUCZ010000001.1  58100438-58100605
            1306-1350           JAXUCZ010000001.1  58103095-58103139
            1351-1445           JAXUCZ010000001.1  58105964-58106058
            1446-1708           JAXUCZ010000001.1  58106764-58107026
            1709-1778           JAXUCZ010000001.1  58109180-58109249
            1779-1897           JAXUCZ010000001.1  58111979-58112097
            1898-1959           JAXUCZ010000001.1  58115996-58116057
            1960-2165           JAXUCZ010000001.1  58116154-58116359
            2166-2186           JAXUCZ010000001.1  58118036-58118056
            2187-2332           JAXUCZ010000001.1  58118781-58118926
            2333-2427           JAXUCZ010000001.1  58119222-58119316
            2428-2693           JAXUCZ010000001.1  58120654-58120919
            2694-2837           JAXUCZ010000001.1  58122227-58122370
            2838-3036           JAXUCZ010000001.1  58124516-58124714
            3037-3140           JAXUCZ010000001.1  58127915-58128018
            3141-3227           JAXUCZ010000001.1  58129765-58129851
            3228-3293           JAXUCZ010000001.1  58146210-58146275
            3294-3367           JAXUCZ010000001.1  58146609-58146682
            3368-3486           JAXUCZ010000001.1  58147139-58147257
            3487-3681           JAXUCZ010000001.1  58148514-58148708
            3682-3773           JAXUCZ010000001.1  58149306-58149397
            3774-3959           JAXUCZ010000001.1  58149864-58150049
            3960-4955           JAXUCZ010000001.1  58152336-58153331
            4956-5090           JAXUCZ010000001.1  58161059-58161193
            5091-5379           JAXUCZ010000001.1  58163914-58164202
            5380-5464           JAXUCZ010000001.1  58167226-58167310
            5465-7442           JAXUCZ010000001.1  58168528-58170505
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q12"
     gene            1..7442
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /note="afadin, adherens junction formation factor"
                     /db_xref="GeneID:26955"
                     /db_xref="RGD:708561"
     exon            1..233
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..5618
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /note="protein Af-6; myeloid/lymphoid or mixed
                     lineage-leukemia translocation to 4 homolog;
                     myeloid/lymphoid or mixed-lineage leukemia (trithorax
                     homolog, Drosophila); translocated to, 4; myeloid/lymphoid
                     or mixed-lineage leukemia; translocated to, 4"
                     /codon_start=1
                     /product="afadin"
                     /protein_id="NP_037349.1"
                     /db_xref="GeneID:26955"
                     /db_xref="RGD:708561"
                     /translation="MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMR
                     FYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGER
                     RLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRT
                     LSKKEKKEKKKREKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNP
                     EVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADF
                     AVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPS
                     DKGILVFQLKRRPPDYIPKKMKKHVEGKPLKGKDRADGSGYGSALPPEKLPYLVELSP
                     GRRNHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHC
                     DLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQFGTSHVFKFVDPIQDHVL
                     SKRSVDGGLMVKGPRHKPGAVQETTFELGGDIHSGTALPASRSTTRLDSDRVSSASST
                     AERGMVKPMIRLDQEQDYRRRESRTQDAAGPELMLPASIEFRESSEDSFLSAIINYTN
                     SSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVD
                     QVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHC
                     LQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLF
                     HFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQA
                     TTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAE
                     NTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFC
                     RLVPHTRSPGTWTIYFEGADYESHLMRENTELTQPLRKEPEVITVTLKKQNGMGLSIV
                     AAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRT
                     SSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNG
                     SPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSPYTSGTAAKI
                     TSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPVQNQWPNYEEK
                     PHMHTESDHASIAIQRVTRSQEELREEKVYQLERHRVESGMDRKCDSDMWINQSSSVE
                     SSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPP
                     PAHYTSDFDGISMDLPLPPPPANQAAPQSAQVAAAERKKREEHQRWYEKEKARLEEER
                     ERKRREQERKLGQMRTQSLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRT
                     IERRDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKG
                     DRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARL
                     QDEERRRQQQLEEMRKREVEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAE
                     RRRQHEEAARRLLEPEEPGLSRPPLPQDYEPPSQSSAPSAPPPPPQRNASYLKTQVLS
                     PDSLFTAKFVAYDDDDEEENYVPAGPNSYSGSAGTTAGTYDAPRDTREKLSRSQDADL
                     PGSSGAPENLTFRERQRLFSQGQDVSDKVKASRKLTELENELNTK"
     exon            234..429
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            430..542
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            543..706
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            707..867
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1025
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1137
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1305
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1350
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1445
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1708
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..1778
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1897
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1898..1959
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1960..2165
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            2166..2186
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2332
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2427
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2693
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            2694..2837
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..3036
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..3140
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3141..3227
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3228..3293
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3294..3367
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3368..3486
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3487..3681
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3682..3773
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3774..3959
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            3960..4955
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            4956..5090
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            5091..5379
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            5380..5464
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
     exon            5465..7442
                     /gene="Afdn"
                     /gene_synonym="Af6; Mllt4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggcgcggg ccggacgcgg gccgagcctg gcggaggacg cgcggcggct gcggcggcgg
       61 cggtggtggt ggtgaggcgg cctgaggtgt tccggcccag cgggtcgcgg agaggagccg
      121 ccgcgatcat gtcggcgggc ggccgcgacg aagagcggcg gaagctggcc gacatcattc
      181 accactggaa cgccaaccgg ctggacctgt tcgagatcag ccagccgacc gaggatttgg
      241 agtttcatgg agtcatgaga ttttattttc aagataaagc tgctggaaac tttgcaacaa
      301 aatgtattcg ggtctctagc acagcaacca ctcaagatgt cattgaaaca ctggcagaga
      361 agttccgccc tgatatgcgc atgctctcct ctcccaagta ctccctctat gaagtgcatg
      421 tcagtggaga aagaagattg gacattgatg agaaacctct agttgtacag ttgaattgga
      481 acaaagatga tcgggagggt agattcgtcc ttaagaacga gaatgacgcc attcctgcca
      541 agaaggctca gagtaatgga cctgagaagc aggagaaaga aggtgttatt cagaacttca
      601 aaagaactct gtcaaagaaa gaaaagaagg aaaaaaagaa gagagagaag gaggctttga
      661 gacaagcctc tgataaggaa gaaaggcctt ctcaagggga tgacagtgag aattctcgtc
      721 tggctgctga agtttacaaa gacatgcctg aaaccagctt tactcgaacg atttctaatc
      781 ctgaagttgt tatgaaacgg cggcggcagc aaaaactgga gaagagaatg caagagtttc
      841 ggagctcaga tgggcggccc gactcgggtg gaacactgag aatctatgca gacagtttaa
      901 aaccaaatat tccatacaag acaatcctgc tgtctactac agatcctgca gactttgctg
      961 tagcagagtc cttagagaag tatggtctgg aaaaagagaa ccccaaggac tactgcattg
     1021 ctcgggttat gcttcctcct ggagcccagc attctgatga gagaggtgct aaggaaataa
     1081 ttcttgatga cgatgaatgc cctttacaaa tcttcaggga atggccaagt gacaaaggga
     1141 ttttagtctt tcagttgaag aggagaccac cggactacat tccaaagaaa atgaagaaac
     1201 atgttgaagg caagccattg aaggggaaag atagagctga cgggtctggc tacggctctg
     1261 ctcttcctcc tgagaagctg ccctacttag tagagctcag cccagggaga aggaatcact
     1321 ttgcctacta cagctatcac acttacgaag atggctctga ctccagagac aagccaaagt
     1381 tataccgcct tcagttaagt gtgactgaag ttgggacaga gaagttcgat gacaattcta
     1441 ttcagctgtt tggtcctgga attcagcccc atcactgtga tctcaccaac atggatggag
     1501 tggtcactgt gacacccaga agtatggatg cagagaccta tgtggatggt cagcgcatct
     1561 cagagaccac aatgctacag agtggcatga gactgcagtt tggcacctcg catgtgttta
     1621 agtttgtgga ccccatccag gaccacgttc tttccaagag atctgtggat ggaggcctga
     1681 tggtcaaggg cccaagacat aaacctggag ccgttcaaga gacaaccttt gaattgggag
     1741 gagatatcca cagtgggaca gcattgccgg caagcaggag cactactaga ttggacagtg
     1801 acagggtgtc ctctgcctct agcacagctg agcgagggat ggtgaagcca atgatccgac
     1861 tggaccagga gcaggactac cggcgaagag aaagcaggac tcaggatgct gctgggcctg
     1921 aactgatgct gcctgccagc attgaattcc gggaaagctc tgaagactct tttttatctg
     1981 ccattataaa ctataccaat agctctacag ttcattttaa gttgtcacct acatatgtgt
     2041 tatacatggc atgccggtat gtattgtcca gccagcacag gcccgacatc agtcctacag
     2101 agcggacaca taaggccatt gctgttgtga acaagatggt gagcatgatg gaaggggtca
     2161 tccaggaagt agaccaggtt gatcagaaac agaagaacat tgcaggagca cttgctttct
     2221 ggatggcaaa tgcatctgag cttctcaact tcatcaagca ggaccgagac cttagtcgga
     2281 ttactctgga tgcccaggat gttctagcac acttggttca gatggcattt aagtacttgg
     2341 ttcactgcct tcagtcagaa cttaataact acatgccagc ctttctggat gatcccgagg
     2401 agaacagtct gcagagacca aaaatagatg atgtgctgca tacgctcaca ggagccatgt
     2461 ccttgttgcg gcgctgcaga gtcaatgccg ccttgaccat ccagctgttc tctcagcttt
     2521 tccacttcat caatatgtgg ctattcaaca gactggtgac tgacccagat tcaggcctgt
     2581 gttcccacta ctggggcgca attatccgcc agcagctggg gcatattgaa gcttgggcag
     2641 agaagcaggg actagagttg gcagctgact gtcacttgag caggatcgtg caggctacta
     2701 cgttgcttac aatggataag tatgtacctg atgacattcc aaatataaac agcacatgct
     2761 ttaagttaaa ttctctgcag cttcaagccc tcttacagaa ttaccactgt gcacctgatg
     2821 agccttttat tcctacggat ctcatagaaa atgtcgtggc tgttgctgag aacacagctg
     2881 atgagcttgc ccgcagtgat ggcagagatg tgcagttaga ggaggaccct gatctacaac
     2941 tgccctttct tctgccagaa gatggctatt cctgtgatgt tgtcagaaac attccaaatg
     3001 gtttacaaga atttttagac cctctgtgcc agagaggatt ttgcaggttg gttcctcaca
     3061 cacgttctcc aggtacctgg acaatatatt ttgaaggagc agattatgaa agtcacctca
     3121 tgcgtgagaa cacagaactg acccagcctc tgaggaagga gcctgaggta atcactgtga
     3181 ccctgaagaa gcagaatgga atgggcctca gcattgttgc agcaaagggt gctggccagg
     3241 ataaactggg aatctacgtt aagtccgttg tcaaaggagg tgctgcagat gtggatggac
     3301 gcctagctgc tggtgaccaa ctccttagtg tggatggacg aagtctggtt ggactttctc
     3361 aggaaagggc agcagaactc atgacaagaa ccagttccgt ggtaacactg gaagttgcaa
     3421 agcaaggcgc tatctatcac ggactggcca ctctccttaa ccagccatca cccatgatgc
     3481 agagaatttc agatcgtcgt ggctcaggta aacccagacc aaagagcgaa ggttttgagc
     3541 tctataataa ttcagctcaa aacggctccc cagagagtcc tcagatgcct tggacagagt
     3601 acagtgagcc aaagaagctg cctggtgacg acaggctgat gaaaaacaga gctgaccacc
     3661 gttccagccc caatgtggca aatcagcccc ctagtcctgg agggaagagt ccatatacct
     3721 ctggaacagc agctaaaata acatctgtct ctactggaaa cctctgcact gaggagcaga
     3781 cccctccacc tagacctgaa gcctatccta tccctactca gacatatacc agagagtatt
     3841 ttacctttcc agcttcaaag tcgcaggatc ggatggctcc tgttcaaaac cagtggccaa
     3901 attatgagga aaaaccacac atgcacacag aaagtgatca tgccagtatt gcaatccagc
     3961 gtgttacccg ttcccaggaa gagcttcggg aagagaaggt ttaccaactt gagcgtcatc
     4021 gagtagagtc aggcatggat cgcaagtgtg acagtgatat gtggatcaat cagagctcct
     4081 cagtggaatc tagcacgtct agccaggagc acctgaacca ttcctccaag tcagttaccc
     4141 ctgcttccac tctgaccaag agtggtcctg gtcgttggaa aaccccagca gctgtgctcc
     4201 ccacccctgt ggctgtctcc caacccattc gaacagacct ccctccacca ccacctccac
     4261 cacctgccca ctacaccagt gattttgatg gtatttcaat ggatttgccc ctcccacctc
     4321 ctcctgccaa ccaagcagct ccccagtctg ctcaggtggc tgctgcagag aggaaaaaga
     4381 gagaggagca tcagaggtgg tatgaaaaag agaaggcccg cctggaggag gaacgagaga
     4441 gaaagcgcag ggagcaagag aggaagttgg ggcagatgcg cactcagtcc ctgaaccctg
     4501 cttccttctc tcctttggcc acacaggcca agccagaaaa gccttccaca ctgcagaggc
     4561 cccaagaaac ggtcattcgg gagctgcagc cccagcagca gccccgtact attgagcgca
     4621 gggatctgca gtacataacc atcagtaaag aggagctgtc ctctggggat agtctgtctc
     4681 cagacccatg gaaacgagat gccagggaga agctggagaa gcaacagcaa atgcacattg
     4741 tagacatgct aagcaaggag atccatgagc tgcaaaacaa gggggaccgt actgcagagg
     4801 agagtgaccg cctgcggaag cttatgctgg agtggcagtt ccagaagaga ctacaggagt
     4861 ccaagcagaa ggatgaggat gatgatgaag aggaagatga tgatgttgac accatgctga
     4921 tcatgcagcg actggaggcg gagcggagag ccaggttgca ggatgaagag cgcaggcgcc
     4981 agcaacagtt ggaagagatg cggaaacgag aagttgaaga cagagtaaga caagaggaag
     5041 atggacgcca tcaggaggag gagcgagtaa agagagatgc tgaagaaaag aggcgacagg
     5101 aagaagggta ttacagccgc ctagaagctg agaggcgcag acagcatgaa gaggcagcac
     5161 gcaggttgct ggagcccgaa gaacccgggc tgagccgacc tccacttcca caggactacg
     5221 agcccccatc ccagtcctca gcacccagtg cccctcctcc cccacctcag cgaaatgcct
     5281 cctacctcaa aacacaggtc ctctccccag actcgctgtt cactgccaag tttgttgcgt
     5341 atgatgatga tgatgaggag gagaactacg tcccagcagg accaaactct tattcgggat
     5401 ctgctggcac aactgctgga acttatgatg ctcctcggga cacaagagaa aaactcagta
     5461 gaagccaaga tgcagactta cctggcagtt ctggagcccc tgaaaacctg acatttagag
     5521 agcgtcagcg cctcttttca caaggtcaag atgtgtcaga caaagtgaaa gcttctcgta
     5581 aattaacaga gcttgagaat gaactcaaca ccaagtgaga agagagaggt cacctgtact
     5641 caagcaggtc tgaccttggc agtggatttg tggggtgggc ctgaagagga aggaacaatt
     5701 gtacttctaa gtgatgttta atttctttct gaaatggttg caattgagag atgttccaaa
     5761 tccaagaaac tttgttttac tttaccaaga aaaatcctgc cttgttctta gtgatcggac
     5821 ttcgtgaagt tacctgttct tgttagtttt agggagtctt ggactcaaag ggcagaatga
     5881 agggctgtaa agatgacagg gtgtttcctt taccctatct tctccattgt cattccaagg
     5941 gcagtaggcc tagaaattat ctttccattc tttctccaaa ctccattcag ctacgcaata
     6001 gtgacagctg agcatttcag agcactactg tgtcatagtg gaccctgtct gcaggagaca
     6061 gacctctcag cacaggtgaa atgttgcctt tatcttctgt aatttgcatg aggtggtctg
     6121 aaggagtctt tttatgtaag gtgcacaaca caacattctt gacaagtaag agcctcctga
     6181 tcaattctaa atgtttgaga ttgaatatct tattaataat tcaattttga aaattctaaa
     6241 gaaacatttg tgaatttaat cctagggatt ttataaatac aaatcatcat tagactaatc
     6301 actggtggaa attaaagatg acagttctgt tagaaagtta cctaggttcc aaagtctcta
     6361 gggcgctcta attacagcgc tttcttgcag gtaggagcaa agacgtccga gaagaatggg
     6421 agctcatggg tggcagtcaa acacagctat ctcagaagtt ataaatttag taaagagatt
     6481 tagaaacact gtcaggaaag gaagaagcgt gttgctgtgc ctcctgtgac ttaagtgcta
     6541 taagaacact tgtgtcagtt gagtctgcat ctctttctca gcgtaataga gttatttaat
     6601 tttaattact caattttgca ctgtcattcc tcagctgagt tatgggagaa agaactcagt
     6661 ttgcttattt tgtggtaatg ttgactttat tatttcaagc acattttctc tttgagaatc
     6721 ctctaatgtg ggtccagata atctttctgt atcatactat tctcttacaa atctgtgcat
     6781 caggaaaata tgcacacttt caagtagaac agaggtattt gcaacccaaa aaaccacttt
     6841 taataagagc tgtatgataa aatgttgaaa tacaagtgta aatatttttt atgtttatcc
     6901 agtttcctat ttgaaagtgt gtctttgctt tttcctctgt aagagaatag aatgtcccag
     6961 agagctgatg aaggatttgc acacttgtct tgcccaggaa gtgcattctc cttgtgtctg
     7021 cagcacatgg gcagcctttg atatcataaa aatacctccc gaagaagagc aggtacctgc
     7081 tgtccctagt ccatgggaag acagatgcac tcaagaacct gcaagcatct gcaaactctg
     7141 catgtgtgtt tgtgagacct gtagtgaagt agacatggcc atccctgcac agtgaggtca
     7201 gatgtgaata agtgttgcaa gaacacattg aatagtgtgt attactgtgg ggaaataccc
     7261 aaactgctgt ttccatttct actgtatttc agttgcagcc tatttttaat aaactttgta
     7321 tctatttaag tgtatttgct attgttttca aagtgctgac aaagtttata tttgtaatgc
     7381 cagcattcct ttttcccatt tccatctttc actccttcat taaactgctg agtcattcct
     7441 tc
//
