LOCUS       NM_080900               6442 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus actin filament associated protein 1 (Afap1),
            mRNA.
ACCESSION   NM_080900 XM_008770246 XM_346465
VERSION     NM_080900.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Cho,Y., Silverstein,R., Geisinger,M.T., Martinkovich,S.,
            Corkill,H., Cunnick,J.M., Planey,S.L. and Arnott,J.A.
  TITLE     AFAP1 Is a Novel Downstream Mediator of TGF-beta1 for CCN2
            Induction in Osteoblasts
  JOURNAL   PLoS One 10 (9), e0136712 (2015)
   PUBMED   26340021
  REMARK    GeneRIF: AFAP1 is an essential downstream signaling component of
            TGF-beta1 for Src activation, CCN2 induction and collagen XIIa in
            osteoblasts.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Gharahkhani,P., Burdon,K.P., Fogarty,R., Sharma,S., Hewitt,A.W.,
            Martin,S., Law,M.H., Cremin,K., Bailey,J.N.C., Loomis,S.J.,
            Pasquale,L.R., Haines,J.L., Hauser,M.A., Viswanathan,A.C.,
            McGuffin,P., Topouzis,F., Foster,P.J., Graham,S.L., Casson,R.J.,
            Chehade,M., White,A.J., Zhou,T., Souzeau,E., Landers,J.,
            Fitzgerald,J.T., Klebe,S., Ruddle,J.B., Goldberg,I., Healey,P.R.,
            Mills,R.A., Wang,J.J., Montgomery,G.W., Martin,N.G.,
            RadfordSmith,G., Whiteman,D.C., Brown,M.A., Wiggs,J.L.,
            Mackey,D.A., Mitchell,P., MacGregor,S. and Craig,J.E.
  CONSRTM   Wellcome Trust Case Control Consortium 2, NEIGHBORHOOD consortium
  TITLE     Common variants near ABCA1, AFAP1 and GMDS confer risk of primary
            open-angle glaucoma
  JOURNAL   Nat Genet 46 (10), 1120-1125 (2014)
   PUBMED   25173105
REFERENCE   3  (bases 1 to 6442)
  AUTHORS   Kuo,J.C., Han,X., Hsiao,C.T., Yates,J.R. 3rd and Waterman,C.M.
  TITLE     Analysis of the myosin-II-responsive focal adhesion proteome
            reveals a role for beta-Pix in negative regulation of focal
            adhesion maturation
  JOURNAL   Nat Cell Biol 13 (4), 383-393 (2011)
   PUBMED   21423176
REFERENCE   4  (bases 1 to 6442)
  AUTHORS   Crimaldi,L., Courtneidge,S.A. and Gimona,M.
  TITLE     Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome
            formation
  JOURNAL   Exp Cell Res 315 (15), 2581-2592 (2009)
   PUBMED   19540230
  REMARK    GeneRIF: Results demonstrate that Tks5 plays a central role in the
            recruitment of AFAP-110, p190RhoGAP, and cortactin to drive
            podosome formation.
REFERENCE   5  (bases 1 to 6442)
  AUTHORS   Dorfleutner,A., Cho,Y., Vincent,D., Cunnick,J., Lin,H., Weed,S.A.,
            Stehlik,C. and Flynn,D.C.
  TITLE     Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells
  JOURNAL   J Cell Sci 121 (Pt 14), 2394-2405 (2008)
   PUBMED   18577577
  REMARK    GeneRIF: AFAP-110 phosphorylation and/or dephosphorylation is
            involved in the regulation of podosome stability and lifespan.
REFERENCE   6  (bases 1 to 6442)
  AUTHORS   Lodyga,M., Bai,X.H., Mourgeon,E., Han,B., Keshavjee,S. and Liu,M.
  TITLE     Molecular cloning of actin filament-associated protein: a putative
            adaptor in stretch-induced Src activation
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 283 (2), L265-L274 (2002)
   PUBMED   12114187
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On or before Feb 26, 2021 this sequence version replaced
            XM_008770246.3, NM_080900.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643285.38836.1,
                                           SRR26643290.44650.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               JAXUCZ010000014.1  79080784-79080939   c
            157-285             JAXUCZ010000014.1  79035629-79035757   c
            286-380             JAXUCZ010000014.1  79035188-79035282   c
            381-489             JAXUCZ010000014.1  79026269-79026377   c
            490-701             JAXUCZ010000014.1  79024019-79024230   c
            702-881             JAXUCZ010000014.1  79021410-79021589   c
            882-983             JAXUCZ010000014.1  79008102-79008203   c
            984-1065            JAXUCZ010000014.1  79007463-79007544   c
            1066-1215           JAXUCZ010000014.1  79001307-79001456   c
            1216-1427           JAXUCZ010000014.1  78995022-78995233   c
            1428-1573           JAXUCZ010000014.1  78993248-78993393   c
            1574-1691           JAXUCZ010000014.1  78986716-78986833   c
            1692-1806           JAXUCZ010000014.1  78981663-78981777   c
            1807-1971           JAXUCZ010000014.1  78977748-78977912   c
            1972-2162           JAXUCZ010000014.1  78975772-78975962   c
            2163-2327           JAXUCZ010000014.1  78974330-78974494   c
            2328-6442           JAXUCZ010000014.1  78967961-78972075   c
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q21"
     gene            1..6442
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /note="actin filament associated protein 1"
                     /db_xref="GeneID:140935"
                     /db_xref="RGD:619959"
     exon            1..156
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            157..285
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..2354
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /note="AFAP-110; 110 kDa actin filament-associated
                     protein"
                     /codon_start=1
                     /product="actin filament-associated protein 1"
                     /protein_id="NP_543176.2"
                     /db_xref="GeneID:140935"
                     /db_xref="RGD:619959"
                     /translation="MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRMQSSKGF
                     EVKDHAQKAETNNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPG
                     KAPEYITSNYDSEAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLL
                     RKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKI
                     TQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSASVPVNKAELEKK
                     LSSERPSSDGEGVVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKK
                     KPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHIVSIP
                     LRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGAL
                     HYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCV
                     NGSLKNKKPPASSNGLPVKGKAPSSQQKKVESAGGVKRTASNAEQYKYGKNRVEADAK
                     RLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQVALEDKLKRLEEECK
                     QREAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPI
                     SSCDTSDAEGPLPVNSAAVLKKSQPSSSSSPCRGHVLQKAKEWELKNGT"
     exon            286..380
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            381..489
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            490..701
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            702..881
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            882..983
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1065
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1215
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1427
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1573
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1691
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1806
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1971
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2162
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2327
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
     exon            2328..6442
                     /gene="Afap1"
                     /gene_synonym="Afap"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgcagctcg caccagcggt gtctgggctc cgggtccaga ctgagatcgc tgagctgcgc
       61 agggccccgc cccgggctcc attgttgagg cagcggacgc tccgcggtct gcgctccttt
      121 cggccgtcct gcgctcacga gccactgaat cctgagcaat ggaagaatta atagttgaac
      181 tccgcctgtt cctggagctc ctggaccatg agtacctcac gtcaactgtc agagagaaaa
      241 aggcagtgct taccaacatc ctgcttcgaa tgcagtcatc caagggcttt gaagtgaagg
      301 accatgcaca gaaggcagag accaacaacc tcccggcacc accccagatg cctctgccag
      361 agatccctca gccatggctg cctcctgaca gtgggcctcc gccgttaccg acatcctctc
      421 tcccagaggg ttactatgag gaagctgtgc ccctgagtcc tggaaaagct ccagagtaca
      481 tcacctcaaa ttatgactct gaggccatga gcagttccta tgagtcatat gatgaagagg
      541 aagaggatgg gaaggggaag aaaacgcggc atcagtggcc atcagaggag gcctctatgg
      601 acctggtgaa agatgccaag atctgcgcct tcctgttgcg taagaaacgc ttcggccagt
      661 ggaccaagct gctctgcgtc atcaaagaca ccaagctgct gtgctacaaa agttccaagg
      721 accagcagcc tcagatggag ctgcccctgc agggctgcag catcacgtac atcccgagag
      781 acagcaagaa gaagaaacac gagctgaaaa tcacccagca aggcacagac cctctggtcc
      841 tcgccgtcca gagcaaggag caggccgagc agtggctgaa ggtaatcaaa gaggcctaca
      901 gtggctgcag tgggcccgtg gatccagagt gttctccacc acccagcgcc agtgtccctg
      961 tgaacaaggc agaactggag aagaaattgt cttcagagag gcccagctct gatggagaag
     1021 gtgttgtaga aaacggagtc accacgtgta atggaaaaga acaagcaaag aggaagaaac
     1081 cctccaagtc agaggccaag gggactgtgt ccaaggtcac tgggaagaaa attaccaaga
     1141 tcatcggtct gggaaagaag aagccatcca cagatgagca gacgtcatca gcagaggaag
     1201 atgtgcccac ctgtggctac ctaaatgtgc tctctaacag tcgctggcgg gagcgctggt
     1261 gcagagtgaa ggacagcaag ctcattctcc acaaggaccg ggctgacctg aagacccaca
     1321 tcgtctccat ccctctccgc ggctgcgagg tgattccggg cttggattct aagcacccat
     1381 tgaccttccg gctgcttcga aatggacagg aggtggctgt cctagaggca tcatcttctg
     1441 aagacatggg cagatggatt ggcatcttac tggctgagac agggtcctca acggacccag
     1501 gagccctaca ctatgactac atagatgtgg agatgtcagc aaatgtcatc cagacggcta
     1561 aacagacctt ctgctttatg aacaggaggg cagtgtccac tagcccgtac ctggggagcc
     1621 tctccaatgg ctatgcccat cccagtggga cagcactaca ctatgacgac gtcccttgtg
     1681 tcaacggctc gttgaagaac aaaaaacctc cagcgtcatc taatgggctt cctgtgaagg
     1741 gaaaggctcc cagcagccag caaaaaaagg tggaatctgc ggggggcgtg aagcgcacag
     1801 catcaaatgc cgagcagtac aaatatggta agaaccgggt agaagcagat gccaagcggt
     1861 tacagtccaa agaggaagag ctactgaaga gaaaagaggc cctgcggaac aggctggcac
     1921 agctccggaa agagaggaag gacctgaggg ctgccatcga agtgaatgca ggcaggaaga
     1981 cccaggtggc cctggaggac aagttgaaga ggttggagga agagtgcaag cagagggagg
     2041 cagagcgggt cagcctggag ctggagctga cggaggtcaa ggagagcctg aagaaggcct
     2101 tggcaggtgg cgtcactcta ggactggcta tcgaacccaa gtcagggaca tcaagcccac
     2161 agtctccagt gtttcggcat cggactctgg aaaactcccc catctccagc tgtgatacaa
     2221 gcgatgctga ggggcctttg cctgtgaaca gtgcagccgt cctgaagaag agccagccgt
     2281 cctccagcag ctccccttgc cgtgggcacg tgctgcagaa ggccaaggag tgggagctga
     2341 agaatggaac atagagggag cccagccaca cccagagact gtgtactgcc accagccttg
     2401 catgtgacac gtgcgcctcc caaagtggcc cgagcatgac tgcagaagtt tagaccgagt
     2461 ctgacgctga cccttggctg gcttgcgtgc acgctttcta ctgtacgacg gtgcccttta
     2521 ggaaagttta ctttaaaact ttggtgtcca aatttacttg ttaagaagca gaaatgaaag
     2581 ggattgacaa gcggttttga agcaccctct agcagatggg gtggttctga ggacctcagc
     2641 tcagtcccaa cctgcagccc acggaccgtg cgcccctgtg agacggggtg ggccaagcct
     2701 ttcccctgtg tggtcagagc tgcatacctc accctgtttg caagagggtc ctactctgca
     2761 gcagcagcat gagctcctgt gtctctgcat acacacctct ccccaccccc tcccccttcc
     2821 cctccccctc cccttccctt cctgtcccct gtcactggag gtgaagccat gaggaatagt
     2881 gctgtgaagc gactttaaga ttcagttttg gtattggaca agccttttgt caccaggata
     2941 gagatgttgt cattcttttt accagggttt ttcatgtttg ttttaaagaa aatgcccctg
     3001 ccctgcccct tttcatcatg tgctgtgctt ggaccttaag gacttttcag agaccctgtt
     3061 tccttcccac tgtatgatgt actttgagag gctgcacaga gctttcagtc cttggctagc
     3121 atgtacccct ttctcctgac ctgagacccc agcttcccag gcaggggctc ctgagcccag
     3181 gaagcacacc ggccaccatc tgcctatggg tgtaacagag caggttctag aacctgtagt
     3241 ttaacctggc ccatctgtga acaaagacat tccttccaga gctccttcct caagtttaca
     3301 ctcgaagctg tagatcaggg tgcactccca tgccgaggtt cggattgcat gggcccttcc
     3361 cactctcccg tctgcctctg ggcatttcat aggatcgttc agctgctcgg gatgcatcgt
     3421 ctgcaggtca tgggggctag cctctaccct aggagtttga gattcaaact ggctttggga
     3481 gcttccagag tacagtgcga acctctccac catggggaga tgcaggcagt gacctggaca
     3541 ttaggcatct ctgcagactt gtgcagggcc agttatacac agtgagaacc tagtgatgcc
     3601 tttccatcgc agtccagaat gtccttagca cacacgggag gaaagtctcc caggctccct
     3661 gcagaggggc cttctctggg cagccttcat atccaggatt ggtgctctgg gcttgagccc
     3721 tgcagcagct gccttggagt cacaaaagag cagcaaggtg gttaccgggg tacagggaca
     3781 ggaaagctac tcagctgaag gtctgtccca ggctagccca ctgtaagacc cttgccaagc
     3841 tgtgcaatgg tgcttctctc tagtgccttc ctccaaaggg cagttctgat tcttcacagc
     3901 agcatctgtg tgggctggtg tctgccagga atctgtgtag aaagaccagg aggaggcata
     3961 cagcctccac acacaccagc ctactcacgg agctgcaact gggggagccc cttgggtcat
     4021 cgcagggagc tgtgaccatt ccatgctctg aaagggaaga gaatttaatg tgccatagac
     4081 ttctgctgcc tcgggtctca gagcagtgct tttggtcact ccaataacag ccttctttgc
     4141 caggaaacct ggtccaggct ttgactgtta ccaaaacaag gagaaaatgc ccaacttgac
     4201 agccagtcct ggccagcata gagggacata gtgaagtatc gtgtccagca gataaacctc
     4261 agatgagttt gtcctgatga aaacacagca gcctccaggc tcctggcatt cttagtttgt
     4321 gctgagaaac ccaggcctgt aatcctttca ctgtgccgga tgctacaaat tccttagaac
     4381 catataaagt aggtcctgga gaagttacgg ctcccaacac agggctccac tgtcctgtgg
     4441 tcccattaat gttctgagat ttggtcagaa agctagttca aagcatttgt ttcaaaataa
     4501 gttatttatt tgtatatgtt caataaatag agttttaatt tatatgtaca tattgggaca
     4561 cccatgatcc tgtcaggatt ctcagtgctc tcattctatg actctcccaa agccaccggc
     4621 atgaccccag ccatcgatgg caggtttgct ttgtgtggat ccaggtaggc agagggctga
     4681 accagcccct ttctgtgagc aaccaggtcc tctgcaccat gggagatgct ccacctcttg
     4741 gggcctctct ttgaggccac agagtttccc tgggtctact tttgctacaa cttggcaaga
     4801 tgtcatcagc tttcacaaac ccctactttt atgtccgttt tctaactaac catttgcatg
     4861 caaatatcat gctggcccgg ggatttgctc tggtccctgg cctgggcatg tgggtgcaac
     4921 cctcagtgtc acacaagctc atgtaccagt gttcttgcaa gggtgagtgt gggtcaaaca
     4981 ggtcatccca ggtgccagag tgacctgtca ggacggtatg gacccacttc acacagaatc
     5041 ttccagagtg cttgctgctc ccctgcctca gcgtcagtgc ccggtcgctt ccccgtgcat
     5101 gccttggtcc tgcctcacca tcctgtccac ttcatctgtc cactgctcca atttcccttt
     5161 ggctgtttct gtgtcagctc gcccatgcca atgggacttt tagaaaatcg gctttggaaa
     5221 gcaatgtccc aggccctgag gacatcgagg aaggcttcca atatgtgggg actgcagtcc
     5281 cccaggtaga attcacacac aggcagcagc tggactgtgt gtccctggcc tctcctccca
     5341 aaggcaaggg acaagccttc cctgtcgttg acctctgccc tgtgctctgg ggaatacttg
     5401 agccaagtta agacattcac acccaccctc accagcaagc aggacacctt caaggtccat
     5461 tctatgggtg gggcaacatc aaaatgattg atctttctgg gttttttttt ctaaagtaat
     5521 tttgaaatag agacatttca aatccatgac atagttttta tagaagcaac tgaatgtctg
     5581 ggtcttgttc ccactgactt actactaatc acgccagagg gctgggtttc tactgtgaac
     5641 taaactgaaa ctaaactccc tcactccccg tgccatcctc acacagctca gccccaggga
     5701 gagcggaagc agcttggcca tgtcactggc gtgcaaagcc ctcccctcag ttttgaggaa
     5761 caccctttgc cagtaggtgg ggatatacca ccatgatctt tggaaacctg ggcttcctgt
     5821 acaccagatt ggattgtcct ttcaaaatgg tgtctttagt tcataattct tagggaccaa
     5881 aggtgttttt aataggataa tgttttaatt gatttctaac aaaggtttac tctacatttt
     5941 aataccagtt tttttacatt ctagatattc caaatcaatt gctcgtggct ctaagttgta
     6001 aaatgttttc acgttatgaa tgtaattatt tccttaatgt atttttaaaa aaaaactaaa
     6061 atcatattta aaataacctt ggaaagacaa caggcgaagg aaagagaaag ttttttttta
     6121 attaactatg ttggtagccc atttgaaatg actgtaaata tattttgatt gtgtagctca
     6181 atgtatttaa ttcagaaagt aaaaattttt tatgtaaaga gacttgcaat gaaaaatcct
     6241 agttttcagt ccatgcccct atcttgtagt cactccctcc caacttttct cctctacaaa
     6301 ctgatttttt ttctactgtt gtaaccgtgt acccatcgac tggcgcccat tggcggaact
     6361 tgtttgtgta ttaataaaac tgtgctatgt agtccggcct tgtgtcctcc tgacagtggc
     6421 tcatggtgca gaagaagttg tt
//
