LOCUS       NM_012490               1504 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus acrosin (Acr), mRNA.
ACCESSION   NM_012490
VERSION     NM_012490.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Isotani,A., Matsumura,T., Ogawa,M., Tanaka,T., Yamagata,K.,
            Ikawa,M. and Okabe,M.
  TITLE     A delayed sperm penetration of cumulus layers by disruption of
            acrosin gene in rats
  JOURNAL   Biol Reprod 97 (1), 61-68 (2017)
   PUBMED   28859281
  REMARK    GeneRIF: This study showed that that acrosin disrupted spermatozoa
            possessed an equal ability to penetrate the zona pellucida with
            wild-type spermatozoa, but the cumulus cell dispersal was slower
            compared to wild-type and heterozygous spermatozoa.
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   3  (bases 1 to 1504)
  AUTHORS   Furlong,L.I., Veaute,C. and Vazquez-Levin,M.H.
  TITLE     Binding of recombinant human proacrosin/acrosin to zona pellucida
            glycoproteins. II. Participation of mannose residues in the
            interaction
  JOURNAL   Fertil Steril 83 (6), 1791-1796 (2005)
   PUBMED   15950652
REFERENCE   4  (bases 1 to 1504)
  AUTHORS   Nayernia,K., Drabent,B., Meinhardt,A., Adham,I.M., Schwandt,I.,
            Muller,C., Sancken,U., Kleene,K.C. and Engel,W.
  TITLE     Triple knockouts reveal gene interactions affecting fertility of
            male mice
  JOURNAL   Mol Reprod Dev 70 (4), 406-416 (2005)
   PUBMED   15685642
REFERENCE   5  (bases 1 to 1504)
  AUTHORS   Kwitek,A.E., Gullings-Handley,J., Yu,J., Carlos,D.C., Orlebeke,K.,
            Nie,J., Eckert,J., Lemke,A., Andrae,J.W., Bromberg,S., Pasko,D.,
            Chen,D., Scheetz,T.E., Casavant,T.L., Soares,M.B., Sheffield,V.C.,
            Tonellato,P.J. and Jacob,H.J.
  TITLE     High-density rat radiation hybrid maps containing over 24,000
            SSLPs, genes, and ESTs provide a direct link to the rat genome
            sequence
  JOURNAL   Genome Res 14 (4), 750-757 (2004)
   PUBMED   15060019
REFERENCE   6  (bases 1 to 1504)
  AUTHORS   Lister,P.
  TITLE     Implementing Project 2000 in a context of change
  JOURNAL   Sr Nurse 12 (2), 34-36 (1992)
   PUBMED   1299864
REFERENCE   7  (bases 1 to 1504)
  AUTHORS   Nagdas,S.K., Skudlarek,M.D., Orgebin-Crist,M.C. and Tulsiani,D.R.
  TITLE     Biochemical alterations in the proacrosin-acrosin system during
            epididymal maturation of the rat spermatozoa
  JOURNAL   J Androl 13 (1), 36-43 (1992)
   PUBMED   1551805
REFERENCE   8  (bases 1 to 1504)
  AUTHORS   Klemm,U., Flake,A. and Engel,W.
  TITLE     Rat sperm acrosin: cDNA sequence, derived primary structure and
            phylogenetic origin
  JOURNAL   Biochim Biophys Acta 1090 (2), 270-272 (1991)
   PUBMED   1932123
REFERENCE   9  (bases 1 to 1504)
  AUTHORS   Kremling,H., Flake,A., Adham,I.M., Radtke,J. and Engel,W.
  TITLE     Exon-intron structure and nucleotide sequence of the rat proacrosin
            gene
  JOURNAL   DNA Seq 2 (1), 57-60 (1991)
   PUBMED   1802037
REFERENCE   10 (bases 1 to 1504)
  AUTHORS   De Jonge,C.J., Mack,S.R. and Zaneveld,L.J.
  TITLE     Inhibition of the human sperm acrosome reaction by proteinase
            inhibitors
  JOURNAL   Gamete Res 23 (4), 387-397 (1989)
   PUBMED   2550339
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC097345.1.
            
            On Mar 8, 2007 this sequence version replaced NM_012490.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC097345.1, X59254.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1504              BC097345.1         1-1504
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..1504
                     /gene="Acr"
                     /gene_synonym="Acro"
                     /note="acrosin"
                     /db_xref="GeneID:24163"
                     /db_xref="RGD:2024"
     exon            1..97
                     /gene="Acr"
                     /gene_synonym="Acro"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..1334
                     /gene="Acr"
                     /gene_synonym="Acro"
                     /EC_number="3.4.21.10"
                     /note="acrosin prepropeptide"
                     /codon_start=1
                     /product="acrosin precursor"
                     /protein_id="NP_036622.2"
                     /db_xref="GeneID:24163"
                     /db_xref="RGD:2024"
                     /translation="MVEMLPTVVALVLAVSVVAKDNTTCDGPCGLRFRQNPQAGIRIV
                     GGQTSSPGAWPWMVSLQIFTSHNSRRYHACGGSLLNSHWVLTAAHCFDNKKKVYDWRL
                     VFGAHEIEYGRNKPVKEPQQERYVQKIVIHEKYNAVTEGNDIALLKVTPPVTCGDFVG
                     PGCLPHFKSGPPRIPHTCYVTGWGYIKDNAPRPSPVLMEARVDLIDLDLCNSTQWYNG
                     RVTSTNVCAGYPEGKIDTCQGDSGGPLMCRDSVDSPFVIVGITSWGVGCARAKRPGVY
                     TATWDYLDWIASKIGPTALHLIQPATPHPPTTQQPVISFHPPSIHPPWYFQHLSPRPP
                     HLRPPRPLLHQPSSVHTSSAPVIPLLSLLTPVQPVSFTLAAYHTRHHTTLSFARRLQH
                     LIEALKMRTYPIKYPSRYSGPVNYQHRFSTFEPLSNKPSEPLLHS"
     exon            98..304
                     /gene="Acr"
                     /gene_synonym="Acro"
                     /inference="alignment:Splign:2.1.0"
     exon            305..588
                     /gene="Acr"
                     /gene_synonym="Acro"
                     /inference="alignment:Splign:2.1.0"
     exon            589..734
                     /gene="Acr"
                     /gene_synonym="Acro"
                     /inference="alignment:Splign:2.1.0"
     exon            735..1504
                     /gene="Acr"
                     /gene_synonym="Acro"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggccaggtta gggcaggagt atggtagaga tgctgccaac tgtcgttgcg ctggtcctgg
       61 cagtgtccgt ggttgccaag gataacacca cgtgtgatgg cccctgtggg ttacgattca
      121 ggcagaaccc acaagcaggt atccggattg tcggagggca gacttcgtcg cctggggcct
      181 ggccctggat ggtcagttta cagatcttca cgtcccataa cagccgtagg tatcacgcct
      241 gcggaggcag cctactgaac tcccactggg tgctcacggc tgctcactgc ttcgataaca
      301 aaaaaaaagt ctatgactgg agactggttt ttggagccca tgaaattgaa tacggaagaa
      361 acaagccagt gaaagagccc cagcaggaaa gatacgtgca gaaaattgtc atccacgaga
      421 aatacaacgc tgtgaccgag gggaacgaca ttgccctctt gaaagtcact cctcctgtta
      481 catgtgggga cttcgttggg cctggctgcc tacctcattt taagtctggt cctcccagaa
      541 tcccccacac ctgctacgtg actgggtggg gatacataaa agataacgcc cccagaccat
      601 cacctgtcct gatggaggcc cgcgtggatc tcattgacct cgacctgtgt aactccaccc
      661 agtggtacaa tgggcgtgtc acatcaacta atgtgtgcgc agggtatcct gaaggcaaga
      721 ttgacacctg ccagggggac agtggtgggc ctctcatgtg cagagacagc gtcgacagcc
      781 cctttgtgat cgtggggatc acgagctggg gggtaggctg tgcccgtgct aagcgtcctg
      841 gagtctacac agccacctgg gactacctgg actggattgc ttccaagatc ggccctaccg
      901 ccttgcactt gattcaaccg gccacccctc acccacctac aacccagcaa ccggtcatct
      961 ctttccaccc tccttcgatc caccctcctt ggtacttcca acacctgtct cctcggccgc
     1021 ctcacctacg accgcctcgg cctctgctcc atcagccgtc ttcagtccat acctcctcag
     1081 ctccagtcat acctctactc tccctgctca ccccagtcca gcctgtgtcc tttacccttg
     1141 ctgcgtacca cacaaggcac cacacaacgc tgtcttttgc tcggcgtcta cagcatctca
     1201 tagaggccct gaagatgaga acttacccta taaaatatcc ctcccggtac agtggacctg
     1261 tgaactacca gcaccgcttc tccacgttcg agcccctttc caacaaaccc agtgagcccc
     1321 tcctccattc ctgagaaaag agaaaagagt gaaatacaca cacacacaca cacacacaca
     1381 cacacacaca cacacacacg aggacatgct ttctggactt ctgttttctc caactcaacc
     1441 caaatcttcc tccatcaaat ctacgtcctc cctcctcagc tccaataaaa tggaatttct
     1501 cata
//
