LOCUS       NM_016988               2067 bp    mRNA    linear   ROD 27-JUL-2024
DEFINITION  Rattus norvegicus acid phosphatase 2, lysosomal (Acp2), transcript
            variant 1, mRNA.
ACCESSION   NM_016988
VERSION     NM_016988.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Loughran,G., Chou,M.Y., Ivanov,I.P., Jungreis,I., Kellis,M.,
            Kiran,A.M., Baranov,P.V. and Atkins,J.F.
  TITLE     Evidence of efficient stop codon readthrough in four mammalian
            genes
  JOURNAL   Nucleic Acids Res 42 (14), 8928-8938 (2014)
   PUBMED   25013167
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Mannan,A.U., Roussa,E., Kraus,C., Rickmann,M., Maenner,J.,
            Nayernia,K., Krieglstein,K., Reis,A. and Engel,W.
  TITLE     Mutation in the gene encoding lysosomal acid phosphatase (Acp2)
            causes cerebellum and skin malformation in mouse
  JOURNAL   Neurogenetics 5 (4), 229-238 (2004)
   PUBMED   15503243
REFERENCE   3  (bases 1 to 2067)
  AUTHORS   Suter,A., Everts,V., Boyde,A., Jones,S.J., Lullmann-Rauch,R.,
            Hartmann,D., Hayman,A.R., Cox,T.M., Evans,M.J., Meister,T., von
            Figura,K. and Saftig,P.
  TITLE     Overlapping functions of lysosomal acid phosphatase (LAP) and
            tartrate-resistant acid phosphatase (Acp5) revealed by doubly
            deficient mice
  JOURNAL   Development 128 (23), 4899-4910 (2001)
   PUBMED   11731469
REFERENCE   4  (bases 1 to 2067)
  AUTHORS   Lakowska,H., Maciejewski,R., Szkodziak,P. and Staskiewicz,G.
  TITLE     Changes in the activity of lysosomal enzymes in rat kidneys in the
            course of acute pancreatitis
  JOURNAL   Med Sci Monit 7 (6), 1193-1197 (2001)
   PUBMED   11687729
REFERENCE   5  (bases 1 to 2067)
  AUTHORS   Tanino,H., Yoshida,J., Yamamoto,R., Kobayashi,Y., Shimohama,S. and
            Fujimoto,S.
  TITLE     Abundance of low molecular weight phosphotyrosine protein
            phosphatase in the nerve-ending fraction in the brain
  JOURNAL   Biol Pharm Bull 22 (8), 794-798 (1999)
   PUBMED   10480315
REFERENCE   6  (bases 1 to 2067)
  AUTHORS   Saftig,P., Hartmann,D., Lullmann-Rauch,R., Wolff,J., Evers,M.,
            Koster,A., Hetman,M., von Figura,K. and Peters,C.
  TITLE     Mice deficient in lysosomal acid phosphatase develop lysosomal
            storage in the kidney and central nervous system
  JOURNAL   J Biol Chem 272 (30), 18628-18635 (1997)
   PUBMED   9228031
REFERENCE   7  (bases 1 to 2067)
  AUTHORS   Cirri,P., Caselli,A., Manao,G., Camici,G., Polidori,R., Cappugi,G.
            and Ramponi,G.
  TITLE     Kinetic studies on rat liver low M(r) phosphotyrosine protein
            phosphatases. The activation mechanism of the isoenzyme AcP2 by
            cGMP
  JOURNAL   Biochim Biophys Acta 1243 (1), 129-135 (1995)
   PUBMED   7827101
REFERENCE   8  (bases 1 to 2067)
  AUTHORS   Himeno,M., Fujita,H., Noguchi,Y., Kono,A. and Kato,K.
  TITLE     Isolation and sequencing of a cDNA clone encoding acid phosphatase
            in rat liver lysosomes
  JOURNAL   Biochem Biophys Res Commun 162 (3), 1044-1053 (1989)
   PUBMED   2764916
REFERENCE   9  (bases 1 to 2067)
  AUTHORS   Lemansky,P., Gieselmann,V., Hasilik,A. and von Figura,K.
  TITLE     Synthesis and transport of lysosomal acid phosphatase in normal and
            I-cell fibroblasts
  JOURNAL   J Biol Chem 260 (15), 9023-9030 (1985)
   PUBMED   3160696
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB720349.1 and BC081823.1.
            
            On Aug 18, 2012 this sequence version replaced NM_016988.1.
            
            Summary: The protein encoded by this gene belongs to the histidine
            acid phosphatase family, which hydrolyze orthophosphoric monoesters
            to alcohol and phosphate. This protein is localized to the
            lysosomal membrane, and is chemically and genetically distinct from
            the red cell acid phosphatase. Mice lacking this gene showed
            multiple defects, including bone structure alterations, lysosomal
            storage defects, and an increased tendency towards seizures. An
            enzymatically-inactive allele of this gene showed severe growth
            retardation, hair-follicle abnormalities, and an ataxia-like
            phenotype. Two isoforms are predicted to be produced from the same
            mRNA by the use of alternative in-frame translation termination
            codons via a stop codon readthrough mechanism. [provided by RefSeq,
            Oct 2017].
            
            Transcript Variant: This variant (1) encodes two isoforms, which
            result from the use of alternative in-frame translation termination
            codons. The shorter isoform (1) results from translation
            termination at the upstream UGA stop codon, while the longer
            isoform (1x) results from UGA stop codon readthrough to the
            downstream UAG termination codon. This RefSeq represents the
            shorter isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081823.1, M27893.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-524               CB720349.1         1-524
            525-2067            BC081823.1         496-2038
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q24"
     gene            1..2067
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /note="acid phosphatase 2, lysosomal"
                     /db_xref="GeneID:24162"
                     /db_xref="RGD:2021"
     exon            1..151
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..1309
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /EC_number="3.1.3.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; lysosomal acid phosphatase; LMW-PTP-II low
                     molecular weight phosphotyrosine protein phosphatase
                     isozyme 2; Acid phosphatase 2, lysozymal"
                     /codon_start=1
                     /product="lysosomal acid phosphatase isoform 1 precursor"
                     /protein_id="NP_058684.2"
                     /db_xref="GeneID:24162"
                     /db_xref="RGD:2021"
                     /translation="MAGRQSGWSQAALLQFLLGMCLMVMPPIQARSLRFVTLLYRHGD
                     RSPVKAYPKDPYQEEKWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNASYHRQEVYV
                     RSTDFDRTLMSAEANLAGLFPPTEVQHFNPNISWQPIPVHTVPITEDRLLKFPLGPCP
                     RYEQLQNETRQTPEYQNMSIQNAQFLDMVANETGLMNLTLETIWNVYDTLFCEQTHGL
                     LLPPWASPQTVQRLSQLKDFSFLFLFGIHDQVQKARLQGGVLLAQILKNLTLMATTSQ
                     FPKLLVYSAHDTTLVALQMALNVYNGKQAPYASCHIFELYQEDNGNFSVEMYFRNDSK
                     KAPWPLTLPGCPHRCPLQDFLRLTEPVIPKDWQKECQLASDTADTEVIVALAVCGSIL
                     FLLIVLLLTVLFRMQAQPPGYHHVADREDHA"
     exon            152..247
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            248..334
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            335..487
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            488..586
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            587..676
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            677..809
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            810..892
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            893..999
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1175
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..2042
                     /gene="Acp2"
                     /gene_synonym="LAP"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2042
                     /gene="Acp2"
                     /gene_synonym="LAP"
ORIGIN      
        1 ggcgttccgg gctcttgctg cacagcctac aacggtgatg gccggcagac agtctggttg
       61 gagccaggcg gctcttctcc agttccttct tggcatgtgc ctaatggtga tgccacccat
      121 acaagcccgg agtctgcgct ttgttacctt gctgtatcga cacggagatc ggtcaccagt
      181 gaaggcatat cctaaggacc cctatcagga agagaaatgg ccccagggat ttggtcagct
      241 aaccaaggaa gggatgctac agcattggga gctgggccag gccctgcggc aacgctacca
      301 tggctttctg aacgcctctt accacaggca agaggtttac gtgcgaagca cagactttga
      361 ccgtactctc atgagtgcag aggccaacct ggccggactc ttccctccca ctgaagttca
      421 gcacttcaac ccgaacattt catggcagcc tatccctgtc cacaccgtgc ccattactga
      481 agacaggttg ctgaagtttc ctttgggtcc atgtccccgt tatgagcagt tgcagaacga
      541 gactcggcag acaccagagt atcagaacat gagtattcag aatgcacaat ttctggacat
      601 ggtggccaat gagacagggc ttatgaactt gaccctagag accatctgga atgtgtatga
      661 cacactcttt tgtgagcaaa cacatgggct gctcctgcca ccctgggcct ctccccaaac
      721 cgtgcagcgt ctgagccagc taaaggactt cagcttcctc ttcctcttcg ggatccacga
      781 tcaagtacag aaggcccggc ttcagggggg agttctgctg gctcaaatat tgaagaatct
      841 gaccctaatg gcaactacct ctcaattccc taagcttctg gtttattctg cgcatgacac
      901 taccctggtt gctctgcaaa tggcactgaa tgtctacaat ggtaaacaag ccccctatgc
      961 ttcctgccac atatttgaac tgtaccagga agataatggg aatttctcag tcgagatgta
     1021 ctttcggaat gacagtaaga aggcaccctg gccactgacc ctgcctggct gtcctcaccg
     1081 ttgcccactg caggacttcc ttcgcctcac agaacctgtc atacccaagg actggcagaa
     1141 ggagtgccag ctagcaagcg atactgcaga cacagaggtg attgtggcac tggctgtctg
     1201 tggctccatc ctcttccttc taatagtgtt gctcctcact gtcctcttcc ggatgcaggc
     1261 ccagcctcct ggctaccacc atgttgcaga cagggaagac catgcttgac aaccactcag
     1321 tccccttccc tctgcctcct aggggatgtg ggctgagtcc ttgctcctga ctattgccga
     1381 tcccagccca gggacaggtt tagccttcct gattacctac gcccaaatga atgagtgagg
     1441 ctgggctggt cgcacgtcac ctgtgactta ccatcctcat gcctgatgtt taccatgtac
     1501 tgtgttggac actggctttc tctaaatggg atttgcctct tctatactcc ctaaggactt
     1561 gagatacaga caagcattca ggttttactc aagacctggg attaaaaaaa caaaacaaca
     1621 acagaaaaaa aaccccaaac cctgaagaag ctggtacttg atctgtctag ctcttccccc
     1681 aagccttgct ctttgtcttc tagcccatat ctttggcaaa tggcttggag gacattgtcc
     1741 cttggtactt ttgcagttca tataaatggc tcattttata tggatcaccc aacagcaaag
     1801 tttccctctg actgaggctg ccgtcagtgt caacctcagg caggttgaag gacactggtg
     1861 ctgcacctgg atgacagtgg gaggacacag gagtgaaaca catggaatca tgacgtgact
     1921 ctccctcagc tgggctggac tcttcaggga cttgaagtaa atgcagctgg tgaactgtac
     1981 accttgactg tggcccgaaa cagagctgaa gtaactccga ggaaatgaag gttttgactg
     2041 agaaaaaaaa aaaaaaaaaa aaaaaaa
//
