LOCUS       NM_024398               3287 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus aconitase 2 (Aco2), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_024398
VERSION     NM_024398.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Wei,J., Zhang,J., Wei,J., Hu,M., Chen,X., Qin,X., Chen,J., Lei,F.
            and Qin,Y.
  TITLE     Identification of AGXT2, SHMT1, and ACO2 as important biomarkers of
            acute kidney injury by WGCNA
  JOURNAL   PLoS One 18 (2), e0281439 (2023)
   PUBMED   36735737
  REMARK    GeneRIF: Identification of AGXT2, SHMT1, and ACO2 as important
            biomarkers of acute kidney injury by WGCNA.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Kim,S.J., Cheresh,P., Williams,D., Cheng,Y., Ridge,K.,
            Schumacker,P.T., Weitzman,S., Bohr,V.A. and Kamp,D.W.
  TITLE     Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced
            mitochondrial DNA damage in alveolar epithelial cells
  JOURNAL   J Biol Chem 289 (9), 6165-6176 (2014)
   PUBMED   24429287
  REMARK    GeneRIF: Ogg1 chaperoning of Aco-2 in preventing oxidant-mediated
            mtDNA damage and apoptosis may afford an innovative target for the
            molecular events underlying oxidant-induced toxicity.
REFERENCE   3  (bases 1 to 3287)
  AUTHORS   Zhao,X., Leon,I.R., Bak,S., Mogensen,M., Wrzesinski,K., Hojlund,K.
            and Jensen,O.N.
  TITLE     Phosphoproteome analysis of functional mitochondria isolated from
            resting human muscle reveals extensive phosphorylation of inner
            membrane protein complexes and enzymes
  JOURNAL   Mol Cell Proteomics 10 (1), M110.000299 (2011)
   PUBMED   20833797
REFERENCE   4  (bases 1 to 3287)
  AUTHORS   Zhuravliova,E., Barbakadze,T., Zaalishvili,E., Chipashvili,M.,
            Koshoridze,N. and Mikeladze,D.
  TITLE     Social isolation in rats inhibits oxidative metabolism, decreases
            the content of mitochondrial K-Ras and activates mitochondrial
            hexokinase
  JOURNAL   Behav Brain Res 205 (2), 377-383 (2009)
   PUBMED   19616040
REFERENCE   5  (bases 1 to 3287)
  AUTHORS   Lin,G., Brownsey,R.W. and MacLeod,K.M.
  TITLE     Regulation of mitochondrial aconitase by phosphorylation in
            diabetic rat heart
  JOURNAL   Cell Mol Life Sci 66 (5), 919-932 (2009)
   PUBMED   19153662
  REMARK    GeneRIF: These results demonstrate the regulation of mitochondrial
            aconitase activity by protein kinase C-dependent phosphorylation.
REFERENCE   6  (bases 1 to 3287)
  AUTHORS   Andersson,U., Leighton,B., Young,M.E., Blomstrand,E. and
            Newsholme,E.A.
  TITLE     Inactivation of aconitase and oxoglutarate dehydrogenase in
            skeletal muscle in vitro by superoxide anions and/or nitric oxide
  JOURNAL   Biochem Biophys Res Commun 249 (2), 512-516 (1998)
   PUBMED   9712727
REFERENCE   7  (bases 1 to 3287)
  AUTHORS   Mirel,D.B., Marder,K., Graziano,J., Freyer,G., Zhao,Q., Mayeux,R.
            and Wilhelmsen,K.C.
  TITLE     Characterization of the human mitochondrial aconitase gene (ACO2)
  JOURNAL   Gene 213 (1-2), 205-218 (1998)
   PUBMED   9630632
REFERENCE   8  (bases 1 to 3287)
  AUTHORS   Chernyshev,V.V., Mavlikaev,R.G., Burtsev,V.I., Dutko,Iu.S. and
            Zhidkov,V.A.
  TITLE     [Aerosol vaccination of broilers against Newcastle disease]
  JOURNAL   Veterinariia (12), 78-80 (1978)
   PUBMED   734909
REFERENCE   9  (bases 1 to 3287)
  AUTHORS   Masini,A., Guarriero-Bobyleva,V., Pincelli-Gatti,A. and Cennamo,C.
  TITLE     Developmental changes of rat liver cytoplasmic and mitochondrial
            aconitate hydratases
  JOURNAL   Ital J Biochem 27 (5), 300-304 (1978)
   PUBMED   755797
REFERENCE   10 (bases 1 to 3287)
  AUTHORS   Slaughter,C.A., Hopkinson,D.A. and Harris,H.
  TITLE     Aconitase polymorphism in man
  JOURNAL   Ann Hum Genet 39 (2), 193-202 (1975)
   PUBMED   1052766
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC061999.1.
            
            On Jan 1, 2004 this sequence version replaced NM_024398.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061999.1, AJ243266.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q34"
     gene            1..3287
                     /gene="Aco2"
                     /note="aconitase 2"
                     /db_xref="GeneID:79250"
                     /db_xref="RGD:621360"
     exon            1..40
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     CDS             5..2347
                     /gene="Aco2"
                     /EC_number="4.2.1.3"
                     /note="aconitate hydratase, mitochondrial; citrate
                     hydro-lyase; aconitase 2, mitochondrial; mitochondrial
                     aconitase (nuclear aco2 gene)"
                     /codon_start=1
                     /product="aconitate hydratase, mitochondrial precursor"
                     /protein_id="NP_077374.2"
                     /db_xref="GeneID:79250"
                     /db_xref="RGD:621360"
                     /translation="MAPYSLLVTRLQKALGVRQYHVASALCQRAKVAMSHFEPSEYIR
                     YDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPANQEIERGKTYLRLRPDRVAMQDAT
                     AQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAK
                     YGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMA
                     GIPWELKCPKVIGVKLTGTLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSIS
                     CTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLAEEFKDHLVPDPGCQY
                     DQVIEINLNELKPHINGPFTPDLAHPVADVGTVAEKEGWPLDIRVGLIGSCTNSSYED
                     MGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGP
                     CIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFN
                     PETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQ
                     LLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINI
                     ENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLG
                     GRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLNC
                     IIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQQ"
     exon            41..177
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            178..436
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            437..529
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            530..688
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            689..839
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            840..944
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1036
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1142
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1300
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1374
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1486
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..1609
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1765
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1957
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2090
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2212
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..3268
                     /gene="Aco2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caaaatggcg ccttacagcc tactggtcac ccggctgcag aaggccctgg gtgtgcggca
       61 gtaccatgtg gcctctgccc tgtgccaacg ggccaaggtg gccatgagcc actttgagcc
      121 cagtgagtac atccgatatg acctgctaga gaagaacatt aacattgtcc gtaaacggtt
      181 gaaccggcct cttactctct cggagaagat tgtgtatgga cacctggatg acccagccaa
      241 ccaggagatc gagcggggaa agacatacct gcgtttacgg cccgaccggg tggccatgca
      301 ggatgcaaca gcccagatgg ctatgctaca gtttattagc agtgggctgc ccaaggtggc
      361 tgtgccatca accatccact gtgatcatct gattgaggcc cagcttgggg gtgagaaaga
      421 cctacgccgg gccaaggaca taaaccagga agtatataat ttcctggcaa ctgccggtgc
      481 caagtatggt gtgggcttct ggaggcctgg atctggaatc attcaccaga tcattctaga
      541 aaactatgca taccctggag ttcttctgat tggcactgac tcccacaccc ccaatggggg
      601 tggcctggga ggtatctgca ttggagtagg gggtgctgat gctgtggatg tcatggctgg
      661 gatcccctgg gagctgaagt gtcccaaggt gattggcgtg aagctgacgg gcaccctctc
      721 tggttggacc tcacccaaag atgtgatcct gaaagtggcc ggcatcctca cagtgaaagg
      781 tggcacaggt gccattgtgg aataccatgg acctggtgtc gactccatct cctgcactgg
      841 catggcaaca atttgcaaca tgggtgcaga aattggggcc actacatcag tgttccccta
      901 caaccacagg atgaaaaagt acctgagcaa gacaggccga gcagacattg ccaacctagc
      961 agaagaattc aaggatcact tagtgcctga ccctggctgc cagtatgacc aagtgattga
     1021 aattaacctc aatgagctaa agccacatat caatggaccc tttacccctg acttggctca
     1081 cccagtggca gatgtgggca ctgtggcaga gaaggaaggg tggcctctgg acatcagagt
     1141 aggtttgatt ggcagctgca ccaattcaag ctacgaggac atgggacgat cagcagctgt
     1201 ggccaagcaa gccctggcgc acggactcaa gtgcaagtct cagttcacca tcaccccagg
     1261 ctctgagcag atccgagcca ctatcgagcg ggatggctat gcacagatcc taagggatgt
     1321 gggtggaatc gttttggcca atgcctgtgg cccttgcatc ggccagtggg acaggaaaga
     1381 catcaagaag ggggagaaga atacaatcgt cacctcctac aacaggaact tcacaggccg
     1441 taatgatgca aatcctgaga cccatgcctt tgtcacatcc ccagagattg tcacagccct
     1501 ggccattgcg ggaaccctta agttcaaccc agaaactgac ttcttaacag gcaaggatgg
     1561 caagaagttc aagttggagg ctccagatgc agatgagctt ccccgatcgg actttgatcc
     1621 agggcaggac acataccagc acccccctaa ggacagcagt ggccagcggg tggatgtgag
     1681 ccctaccagc cagcgcctgc agctcctgga accttttgac aaatgggatg gcaaagacct
     1741 agaggacctg caaatcctta tcaaggtcaa agggaagtgc accacagacc acatttctgc
     1801 tgccggcccc tggctcaagt tccgtgggca tctggataac atctctaata acctgcttat
     1861 cggtgccatc aacatcgaaa atggcaaagc caattctgtg cgcaatgctg tcacccagga
     1921 gtttggccct gtacctgaca ctgctcgcta ctacaagaaa catggcatca ggtgggtggt
     1981 gattggagat gagaactacg gtgaaggctc aagccgggag cacgcagcac tggagcctcg
     2041 ccatctgggg ggccgagcca tcatcaccaa gagcttcgca aggatccatg aaaccaatct
     2101 aaagaagcag ggcttgctgc ccctcacctt tgctgacccc tctgactaca acaagattca
     2161 tccagtggac aagctgacca ttcaaggcct gaaggacttt gcccctggca agcctctgaa
     2221 ctgcatcatc aagcacccca atgggaccca ggagaccatc ctgctgaacc acactttcaa
     2281 cgagacacag atcgagtggt tccgtgcagg cagcgccctc aacaggatga aggagctgca
     2341 gcagtgagga gccaggctgg ctcctgtccc actctggacc ctgctggggc aaaggtgcag
     2401 gcccatgtgc atccacagag cctcatctgg tctgtccagc atgaccacct gctctaagat
     2461 ggtgaccaga cctgcttctt gccctcccta tcctcagccc actgagtgaa ccaacatgga
     2521 gggcacttaa gatgatggag ggcacttaag atgatgtccc agccccgcct tcctgttcag
     2581 tttggtttgt ctttgagcaa cccatgcaac tgtatttatt tttgatgaca gcactccctc
     2641 tacagaagtt tttattctgc ctaatcattt cactggtggc tgaaggattt tagagaaact
     2701 tgttcttata agaaaaaaaa aaagatccaa atctattgag cgtcctgtct gagagtggtt
     2761 ggtgtactgt tttatcagct tgggctgatg aagggttttt caagccgagg tttcatcaag
     2821 cctggtctct gtagcaaagt caggagtgat gacaaaaaaa ctactccatc ccccctgccc
     2881 tgcacatgtg cacatgtgcc tgcaatagac gagaggtgcc tcctcctctg ttcccctgag
     2941 agtagacgca gtacttacct taaagataac gctagtggcg gtggcacaca cctggcagat
     3001 ggatctgagt tcaagtccag tctagactgc agagttaaaa cagcccgaaa aggggctgga
     3061 gagatggctc agtggttaag agcacccaac tgctcttcca gaggtcctga gttcaattcc
     3121 cagcaaccac atggtggctc acaaccatct gtaatgagat ctgatgcctt cttctggtgt
     3181 atctgaagac agctacagtg tacttatata taatgaatga ataaatctta aaaaaataaa
     3241 taaatacaaa gtaaacaaac aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
