LOCUS       NM_017321               3472 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus aconitase 1 (Aco1), mRNA.
ACCESSION   NM_017321 XM_346735
VERSION     NM_017321.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Zhang,X.M., Zhao,Y.Q., Yan,H., Liu,H. and Huang,G.W.
  TITLE     Inhibitory effect of homocysteine on rat neural stem cell growth in
            vitro is associated with reduced protein levels and enzymatic
            activities of aconitase and respiratory complex III
  JOURNAL   J Bioenerg Biomembr 49 (2), 131-138 (2017)
   PUBMED   27914013
  REMARK    GeneRIF: Proteomic analysis revealed that the expression levels of
            two mitochondrial proteins, cytochrome bc1 complex2 (UQCRC2, the
            major component of electron transport chain complex III) and
            aconitase (an enzyme involved in the tricarboxylic acid cycle),
            were decreased by homocysteine in neural stem cells
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Lane,D.J., Merlot,A.M., Huang,M.L., Bae,D.H., Jansson,P.J.,
            Sahni,S., Kalinowski,D.S. and Richardson,D.R.
  TITLE     Cellular iron uptake, trafficking and metabolism: Key molecules and
            mechanisms and their roles in disease
  JOURNAL   Biochim Biophys Acta 1853 (5), 1130-1144 (2015)
   PUBMED   25661197
  REMARK    Review article
REFERENCE   3  (bases 1 to 3472)
  AUTHORS   Liu,Y. and Templeton,D.M.
  TITLE     Iron-dependent turnover of IRP-1/c-aconitase in kidney cells
  JOURNAL   Metallomics 7 (5), 766-775 (2015)
   PUBMED   25652229
  REMARK    GeneRIF: Iron increases turnover of IRP-1 in kidney cells, while
            increasing aconitase activity, suggesting that the apoprotein
            (aconitase-inactive) form is not exclusively responsible for
            turnover.
REFERENCE   4  (bases 1 to 3472)
  AUTHORS   Liu,Y., Xiao,W. and Templeton,D.M.
  TITLE     Cadmium-induced aggregation of iron regulatory protein-1
  JOURNAL   Toxicology 324, 108-115 (2014)
   PUBMED   25106854
  REMARK    GeneRIF: Polymer-dependent translocation of IRP-1 in Cd(2+)-exposed
            cells may underlie effects of Cd(2+) on iron homeostasis.
REFERENCE   5  (bases 1 to 3472)
  AUTHORS   Gammella,E., Maccarinelli,F., Buratti,P., Recalcati,S. and Cairo,G.
  TITLE     The role of iron in anthracycline cardiotoxicity
  JOURNAL   Front Pharmacol 5, 25 (2014)
   PUBMED   24616701
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3472)
  AUTHORS   Philpott,C.C., Klausner,R.D. and Rouault,T.A.
  TITLE     The bifunctional iron-responsive element binding protein/cytosolic
            aconitase: the role of active-site residues in ligand binding and
            regulation
  JOURNAL   Proc Natl Acad Sci U S A 91 (15), 7321-7325 (1994)
   PUBMED   8041788
  REMARK    GeneRIF: Human ACO1 is a moonlighting protein that has both
            aconitase enzyme activity as well as an mRNA binding function.
REFERENCE   7  (bases 1 to 3472)
  AUTHORS   Haile,D.J., Rouault,T.A., Harford,J.B., Kennedy,M.C., Blondin,G.A.,
            Beinert,H. and Klausner,R.D.
  TITLE     Cellular regulation of the iron-responsive element binding protein:
            disassembly of the cubane iron-sulfur cluster results in
            high-affinity RNA binding
  JOURNAL   Proc Natl Acad Sci U S A 89 (24), 11735-11739 (1992)
   PUBMED   1281544
REFERENCE   8  (bases 1 to 3472)
  AUTHORS   Yu,Y., Radisky,E. and Leibold,E.A.
  TITLE     The iron-responsive element binding protein. Purification, cloning,
            and regulation in rat liver
  JOURNAL   J Biol Chem 267 (26), 19005-19010 (1992)
   PUBMED   1527027
REFERENCE   9  (bases 1 to 3472)
  AUTHORS   Kaptain,S., Downey,W.E., Tang,C., Philpott,C., Haile,D.,
            Orloff,D.G., Harford,J.B., Rouault,T.A. and Klausner,R.D.
  TITLE     A regulated RNA binding protein also possesses aconitase activity
  JOURNAL   Proc Natl Acad Sci U S A 88 (22), 10109-10113 (1991)
   PUBMED   1946430
REFERENCE   10 (bases 1 to 3472)
  AUTHORS   Masini,A., Guarriero-Bobyleva,V., Pincelli-Gatti,A. and Cennamo,C.
  TITLE     Developmental changes of rat liver cytoplasmic and mitochondrial
            aconitate hydratases
  JOURNAL   Ital J Biochem 27 (5), 300-304 (1978)
   PUBMED   755797
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Nov 30, 2020 this sequence version replaced NM_017321.1.
            
            Summary: This gene encodes a member of the aconitase/IPM isomerase
            protein family. The encoded protein has been identified as a
            moonlighting protein based on its ability to perform
            mechanistically distinct functions. Depending on iron levels in the
            cytosol, the encoded protein can function as either an aconitase
            enzyme or as an mRNA binding protein. When cellular iron levels are
            high, the encoded protein functions as an aconitase, an essential
            enzyme in the TCA cycle that catalyzes the conversion of citrate to
            isocitrate. When cellular iron levels are low, the encoded protein
            regulates iron uptake and utilization by binding to iron-responsive
            elements in the untranslated regions of mRNAs for genes involved in
            iron metabolism [provided by RefSeq, Jan 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.2354219.1,
                                           SRR22399481.2043645.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s) :: inferred from conservation
            RefSeq Select criteria          :: based on single protein-coding
                                               transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-117               JAXUCZ010000005.1  60055895-60056011
            118-236             JAXUCZ010000005.1  60075175-60075293
            237-405             JAXUCZ010000005.1  60076005-60076173
            406-543             JAXUCZ010000005.1  60078549-60078686
            544-613             JAXUCZ010000005.1  60087609-60087678
            614-797             JAXUCZ010000005.1  60087793-60087976
            798-937             JAXUCZ010000005.1  60088533-60088672
            938-1109            JAXUCZ010000005.1  60090838-60091009
            1110-1210           JAXUCZ010000005.1  60092550-60092650
            1211-1327           JAXUCZ010000005.1  60093477-60093593
            1328-1487           JAXUCZ010000005.1  60094902-60095061
            1488-1623           JAXUCZ010000005.1  60096304-60096439
            1624-1708           JAXUCZ010000005.1  60097143-60097227
            1709-1865           JAXUCZ010000005.1  60098457-60098613
            1866-1990           JAXUCZ010000005.1  60099924-60100048
            1991-2095           JAXUCZ010000005.1  60101692-60101796
            2096-2238           JAXUCZ010000005.1  60102968-60103110
            2239-2386           JAXUCZ010000005.1  60104521-60104668
            2387-2509           JAXUCZ010000005.1  60108727-60108849
            2510-2695           JAXUCZ010000005.1  60109802-60109987
            2696-3472           JAXUCZ010000005.1  60111144-60111920
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q22"
     gene            1..3472
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /note="aconitase 1"
                     /db_xref="GeneID:50655"
                     /db_xref="RGD:2019"
     exon            1..117
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            118..236
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..2809
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /EC_number="4.2.1.3"
                     /note="IRE-BP 1; citrate hydro-lyase; iron regulatory
                     protein 1; iron-responsive element-binding protein 1;
                     aconitase 1, soluble"
                     /codon_start=1
                     /product="cytoplasmic aconitate hydratase"
                     /protein_id="NP_059017.2"
                     /db_xref="GeneID:50655"
                     /db_xref="RGD:2019"
                     /translation="MKNPFAHLAEPLDPAQPGKKFFNLNKLEDSRYGRLPFSIRVLLE
                     AAVRNCDEFLVKKNDIENILNWSIMQHKSIEVPFKPARVILQDFTGVPAVVDFAAMRD
                     AVKKLGGNPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWG
                     SQAFCNMRIIPPGSGIIHQVNLEYLARVVFDQDGCYYPDSLVGTDSHTTMIDGLGVLG
                     WGVGGIEAEAVMLGQPISMVLPQVIGYKLMGKPHPLVTSTDIVLTITKHLRQVGVVGK
                     FVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDNVSIAYLVQTGREEDKVKHIK
                     RYLQAVGMFRDFSDSSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSEMKRDFESC
                     LGAKQGFKGFQVAPDHHNDHKTFIYNDSEFTLAHGSVVIAAITSCTNTSNPSVMLGAG
                     LLAKKAVEAGLNVKPYVKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIG
                     NSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTV
                     RIDFEKEPLGVNAQGQQVFLKDIWPTRDEIQEVERKYVIPGMFKEVYQKIETVNKSWN
                     ALAAPSEKLYAWNPKSTYIKSPPFFESLTLDLQPPKSIVDAYVLLNLGDSVTTDHISP
                     AGNIARNSPAARYLTNRGLTPRDFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQ
                     TVHLPSGETLDVFDAAERYQQAGLPLIVLAGKEYGSGSSRDWAAKGPFLLGIKAVLAE
                     SYERIHRSNLVGMGVIPLEYLPGETADSLGLTGRERYTIHIPEDLKPRMKVQIKLDTG
                     KTFQAVMRFDTDVELTYFHNGGILNYMIRKMAQ"
     exon            237..405
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            406..543
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            544..613
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            614..797
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            798..937
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1109
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1210
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1327
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1487
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1623
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1708
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..1865
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1990
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1991..2095
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2238
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2386
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2509
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2695
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2696..3472
                     /gene="Aco1"
                     /gene_synonym="Acon1; AH; IRP1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccgcctcgc agccggagcg tccggaggac cgctcagggg gaggagcgca aagtcggcct
       61 gggttcttgc ttgcacccgc ctgtgtgctc cgtgctccgt gagccccgct gtgagcgaga
      121 cacgtgaccg tcagtaatca tgaagaatcc atttgcgcac cttgccgagc ccttggaccc
      181 tgcacagcca ggaaagaaat tcttcaactt gaataaattg gaggactcaa gatatggacg
      241 cttaccattc tctatcagag ttctcctgga ggccgccgtt cggaactgtg acgagttttt
      301 ggtgaagaag aatgacattg agaatatcct gaactggagt atcatgcagc ataagagcat
      361 agaagtgccg tttaagccag cccgagtcat actgcaggac tttacgggcg tgcctgctgt
      421 ggttgatttt gcagcaatgc gcgatgctgt gaagaagttg ggaggaaatc cagagaaaat
      481 aaaccctgtc tgccccgctg accttgtaat cgatcattcc atccaggttg atttcaacag
      541 aagggcagac agtttgcaga agaatcaaga cctggaattt gaaaggaata gagaacgatt
      601 tgaattttta aagtggggtt cccaggcctt ttgcaacatg aggattattc ctcctggctc
      661 aggaattatt caccaagtga atctggagta tttggcaaga gtagtgtttg atcaggatgg
      721 atgttactac ccagatagcc tcgtgggcac agattctcac accaccatga ttgatggtct
      781 gggagttctt ggttggggtg taggtggtat tgaagcagaa gctgtcatgc tgggtcagcc
      841 aatcagcatg gtgcttcccc aggtgattgg ctacaagctg atggggaagc ctcaccctct
      901 ggtaacctcc acggacattg tgctcaccat taccaagcac ctccgacaag ttggggtcgt
      961 gggcaaattc gtggagtttt tcgggccagg agtggcccag ctgtccattg ctgaccgagc
     1021 tacgattgcc aacatgtgcc cagagtacgg cgcgacggca gccttcttcc cggtcgacaa
     1081 cgttagcatc gcgtacctgg tgcagacagg tcgtgaggaa gacaaagtaa agcacattaa
     1141 aaggtatctt caggctgtag gcatgtttcg agacttcagt gactcctctc aagacccaga
     1201 cttcactcag gtcgtggagt tagatttgaa aacagttgtg ccttgctgca gtggacctaa
     1261 aagacctcag gacaaagttg ctgtgtctga gatgaaaagg gactttgaaa gctgccttgg
     1321 agccaagcaa ggatttaaag gttttcaagt tgctccagac catcacaatg accacaagac
     1381 gtttatctat aacgacagtg aattcactct tgctcatggc tccgtggtga tcgccgccat
     1441 cactagctgc acaaacacca gcaatccgtc cgtgatgtta ggcgcaggat tgttagcaaa
     1501 gaaagccgta gaggcggggc tgaatgtgaa gccttacgtc aaaaccagcc tgtctcctgg
     1561 gagtggagtg gtcacctact accttcgaga gagtggagtc atgccttacc tgtcccagtt
     1621 agggtttgac gtggtgggct acggctgcat gacctgcatc ggcaacagtg gacccctccc
     1681 cgaacctgtg gtggaggcta tcacccaggg agaccttgtg gctgttgggg tactgtctgg
     1741 aaacaggaat tttgaaggac gagtccatcc taacacccgg gccaactact tagcatctcc
     1801 cccactagta atagcatatg caattgcagg caccgtcagg atcgacttcg agaaagagcc
     1861 tttgggagtg aacgcacagg gccaacaagt gtttctgaag gatatctggc caactcgaga
     1921 tgagatccag gaggtggagc ggaagtatgt catccccggc atgttcaagg aggtctatca
     1981 gaagatagag actgtaaaca aaagctggaa tgccttagca gccccttcag agaagctgta
     2041 tgcgtggaac cccaagtcta cttatatcaa gtcaccgcca ttctttgaaa gcttgacttt
     2101 agatctccag ccccccaagt ctatagtgga tgcctatgtg ctactaaatc taggagattc
     2161 cgtaacaacg gatcatatct ctccagcggg gaacattgca agaaacagcc ctgccgctcg
     2221 ctacttgacg aacagaggcc tgacgccacg agatttcaac tcctacggct cccgccgggg
     2281 taacgacgcc atcatggcac gggggacatt tgccaacatt cgcttgctga acaagtttct
     2341 gaacaagcag gcccctcaga ctgtccacct tccttcagga gaaaccctcg atgtgttcga
     2401 tgccgctgag cggtaccagc aggctggact tcccctgatt gttctggctg gcaaagagta
     2461 cggttcaggc agctcccgag actgggcagc caaaggtcct ttcctgctgg gaatcaaagc
     2521 tgtcctggca gagagctacg agcgcattca tcgcagcaac ctggttggca tgggggtgat
     2581 cccccttgag tatctccccg gcgaaactgc agactctctg ggactcacgg gtcgggaaag
     2641 gtacacgatc cacattcccg aagaccttaa gccccgcatg aaggttcaga taaagctgga
     2701 caccgggaag accttccagg ccgtgatgag gttcgacacc gacgtggagc tcacttactt
     2761 ccacaatgga ggcatcctga actacatgat ccgaaagatg gcccagtagg tgctggcctc
     2821 tcaggagacc cgcgcttggt gctagaccca atgaggtacc aggcctccgc tggtggaggc
     2881 ctggcgagca gccacctcta cttctcgtga gggtgctagc aagatgagca agtgggccct
     2941 gccattcctg gaggctcagc ggcaggagtc tctagttcgg tgatttgtta atctttttat
     3001 ccttttctgt aatccggaat ctagaatcat gggaaggtcc atagtcccaa agagagctac
     3061 cttctcttta aagtcactca tcaccggtca ttgatttttt tcactctgac taatcttcag
     3121 cagaactagc cagtatctca gaagtgtctc ctaccctttc tgttactctg tctgtctgtg
     3181 ctcagtgaca cccttccctg gagagcccat tcctccgtgt atcacaccag tgttaacgac
     3241 atagcttcag actctgtcac acttcaaatt catagtaatc gtgtgatccc ttccttccaa
     3301 gtgagcgaag accttgtggc taggtcggcc gtcccaagtg tttgattacc taccttccaa
     3361 tcaccgtgag ttgtctttta ccattttcaa catttgttga cagggtttga aagtaaccgg
     3421 ggagcgagac aggatttcta attgaataag attaaatata ttttcatgaa aa
//
