LOCUS       NM_001401063            4192 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus alkaline ceramidase 3 (Acer3), mRNA.
ACCESSION   NM_001401063 XM_008759809
VERSION     NM_001401063.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Wang,K., Li,C., Lin,X., Sun,H., Xu,R., Li,Q., Wei,Y., Li,Y.,
            Qian,J., Liu,C., Zhang,Q., Yu,S., Cui,Z., Huang,X., Zhu,B., Zhou,J.
            and Mao,C.
  TITLE     Targeting alkaline ceramidase 3 alleviates the severity of
            nonalcoholic steatohepatitis by reducing oxidative stress
  JOURNAL   Cell Death Dis 11 (1), 28 (2020)
   PUBMED   31949129
  REMARK    Erratum:[Cell Death Dis. 2020 Mar 17;11(3):191. PMID: 32184395]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Vasiliauskaite-Brooks,I., Healey,R.D., Rochaix,P., Saint-Paul,J.,
            Sounier,R., Grison,C., Waltrich-Augusto,T., Fortier,M., Hoh,F.,
            Saied,E.M., Arenz,C., Basu,S., Leyrat,C. and Granier,S.
  TITLE     Structure of a human intramembrane ceramidase explains enzymatic
            dysfunction found in leukodystrophy
  JOURNAL   Nat Commun 9 (1), 5437 (2018)
   PUBMED   30575723
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4192)
  AUTHORS   Yin,Y., Xu,M., Gao,J. and Li,M.
  TITLE     Alkaline ceramidase 3 promotes growth of hepatocellular carcinoma
            cells via regulating S1P/S1PR2/PI3K/AKT signaling
  JOURNAL   Pathol Res Pract 214 (9), 1381-1387 (2018)
   PUBMED   30097213
REFERENCE   4  (bases 1 to 4192)
  AUTHORS   Edvardson,S., Yi,J.K., Jalas,C., Xu,R., Webb,B.D., Snider,J.,
            Fedick,A., Kleinman,E., Treff,N.R., Mao,C. and Elpeleg,O.
  TITLE     Deficiency of the alkaline ceramidase ACER3 manifests in early
            childhood by progressive leukodystrophy
  JOURNAL   J Med Genet 53 (6), 389-396 (2016)
   PUBMED   26792856
REFERENCE   5  (bases 1 to 4192)
  AUTHORS   Wang,K., Xu,R., Snider,A.J., Schrandt,J., Li,Y., Bialkowska,A.B.,
            Li,M., Zhou,J., Hannun,Y.A., Obeid,L.M., Yang,V.W. and Mao,C.
  TITLE     Alkaline ceramidase 3 deficiency aggravates colitis and
            colitis-associated tumorigenesis in mice by hyperactivating the
            innate immune system
  JOURNAL   Cell Death Dis 7 (3), e2124 (2016)
   PUBMED   26938296
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4192)
  AUTHORS   Wang,K., Xu,R., Schrandt,J., Shah,P., Gong,Y.Z., Preston,C.,
            Wang,L., Yi,J.K., Lin,C.L., Sun,W., Spyropoulos,D.D., Rhee,S.,
            Li,M., Zhou,J., Ge,S., Zhang,G., Snider,A.J., Hannun,Y.A.,
            Obeid,L.M. and Mao,C.
  TITLE     Alkaline Ceramidase 3 Deficiency Results in Purkinje Cell
            Degeneration and Cerebellar Ataxia Due to Dyshomeostasis of
            Sphingolipids in the Brain
  JOURNAL   PLoS Genet 11 (10), e1005591 (2015)
   PUBMED   26474409
  REMARK    Erratum:[PLoS Genet. 2018 Jan 23;14 (1):e1007190. PMID: 29360825]
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4192)
  AUTHORS   Xu,R., Sun,W., Jin,J., Obeid,L.M. and Mao,C.
  TITLE     Role of alkaline ceramidases in the generation of sphingosine and
            its phosphate in erythrocytes
  JOURNAL   FASEB J 24 (7), 2507-2515 (2010)
   PUBMED   20207939
REFERENCE   8  (bases 1 to 4192)
  AUTHORS   Hu,W., Xu,R., Sun,W., Szulc,Z.M., Bielawski,J., Obeid,L.M. and
            Mao,C.
  TITLE     Alkaline ceramidase 3 (ACER3) hydrolyzes unsaturated long-chain
            ceramides, and its down-regulation inhibits both cell proliferation
            and apoptosis
  JOURNAL   J Biol Chem 285 (11), 7964-7976 (2010)
   PUBMED   20068046
REFERENCE   9  (bases 1 to 4192)
  AUTHORS   Mao,C., Xu,R., Szulc,Z.M., Bielawska,A., Galadari,S.H. and
            Obeid,L.M.
  TITLE     Cloning and characterization of a novel human alkaline ceramidase.
            A mammalian enzyme that hydrolyzes phytoceramide
  JOURNAL   J Biol Chem 276 (28), 26577-26588 (2001)
   PUBMED   11356846
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Feb 1, 2022 this sequence version replaced XM_008759809.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22582419.601815.1,
                                           SRR26643429.2673.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN12840115,
                                           SAMN12840127 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-193               JAXUCZ010000001.1  162017606-162017798 c
            194-304             JAXUCZ010000001.1  161970434-161970544 c
            305-357             JAXUCZ010000001.1  161966353-161966405 c
            358-410             JAXUCZ010000001.1  161964133-161964185 c
            411-492             JAXUCZ010000001.1  161962585-161962666 c
            493-528             JAXUCZ010000001.1  161958050-161958085 c
            529-587             JAXUCZ010000001.1  161937030-161937088 c
            588-689             JAXUCZ010000001.1  161924264-161924365 c
            690-794             JAXUCZ010000001.1  161921510-161921614 c
            795-840             JAXUCZ010000001.1  161919427-161919472 c
            841-4192            JAXUCZ010000001.1  161915397-161918748 c
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q32"
     gene            1..4192
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /note="alkaline ceramidase 3"
                     /db_xref="GeneID:499210"
                     /db_xref="RGD:1561254"
     exon            1..193
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..894
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /EC_number="3.5.1.23"
                     /note="phytoceramidase, alkaline"
                     /codon_start=1
                     /product="alkaline ceramidase 3"
                     /protein_id="NP_001387992.1"
                     /db_xref="GeneID:499210"
                     /db_xref="RGD:1561254"
                     /translation="MAPAVDRKGYWGPTTSTLDWCEENYVVTLFVAEFWNTVSNLIMI
                     IPPIFGAIQGFRDRLEKRYIAAYVALTVVGMGSWCFHMTLKYEMQLLDELPMIYSCCI
                     FVYCMFECFKTKSSINYHLLFTLVLFSLIVTTIYLKVKEPIFHQVMYGMLVFALVLRS
                     IYIVTWVYPWLRGLGYTSLTVFLLGFLLWNVDNIFCDSLRNFRKTVPPVLGVATQFHA
                     WWHILTGLGSYLHILFSLYTRTLYLRYRPKVKFLFGIWPMVMFEPQRKH"
     exon            194..304
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            305..357
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            358..410
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            411..492
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            493..528
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            529..587
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            588..689
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            690..794
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            795..840
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
     exon            841..4192
                     /gene="Acer3"
                     /gene_synonym="Phca; RGD1561254"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccctttggg taagggagga gggagccgga gagcgcgcgt tgcctgagcc ggcccgagag
       61 cggccgagca gagtgtgggg cgtcggcgcg atggctccgg ctgtggaccg caaaggctat
      121 tggggcccca cgacctccac attggactgg tgtgaggaga attatgtggt gaccttgttc
      181 gtcgctgagt tctggaatac agtgagtaac ctgattatga tcatacctcc aatttttggt
      241 gcaattcaag gctttagaga tagactggag aaacggtaca ttgctgctta cgtagcactc
      301 acagtggtag gaatgggatc ctggtgtttc cacatgactc tgaaatatga aatgcagctg
      361 ttggatgagc tccccatgat ttacagttgc tgcatatttg tatactgcat gtttgagtgt
      421 ttcaagacaa agagctcaat aaactaccat cttcttttta ccctggttct attcagttta
      481 atagtaacca cgatttacct aaaagtaaaa gagcctatat tccatcaggt catgtatgga
      541 atgttggtct ttgcattagt actccgttct atttatattg ttacatgggt ttatccgtgg
      601 ctcagaggac taggttatac atccttgact gtcttcttat tgggattttt attgtggaat
      661 gtagataaca tcttttgtga ttcactgagg aactttcgaa agacagtgcc ccctgtccta
      721 ggtgttgcaa cacagtttca tgcatggtgg catattctaa ctggcctggg ctcttatctc
      781 cacatccttt tcagtttata tacaagaaca ctttacctga ggtacaggcc aaaagtgaag
      841 tttctctttg gaatctggcc catggtcatg tttgaacctc agaggaagca ctgatgaatt
      901 aattattcta ccaagaaaac aaaaagcact tactgtatgt agttctggca agatggtcaa
      961 gacatctcca aagacttgtg catttgaaga caccatgccc atcacaagag atgaatgact
     1021 cagtcacaga gaatgggaga ttggtctgtt gggtgattag ctcctggctt tatctcgttt
     1081 gtcctggacc taagatgctt agagagaaac agatagtgta tatttatatt ctggaatggt
     1141 ggagaagttg gacttttctt aacaggttaa cagtttgtgc tggtcattca tggaaaatta
     1201 atattttttc ttttcttttt tctatttttt atgagttgca tgttaattgt acctttatta
     1261 atttttgatt aaatattgaa cagagtcatt aagtactggc aagatcccat atccagatgg
     1321 tcgactgtga gccagcactt agccgtgtca gctttgactc acattatcac attaacttcg
     1381 cacatgactt tccagtaata aaagataagg tgtgggactc aaggtagctc ctcgctatct
     1441 gccttggtgc tagctggagt cttgtaagca aactccgacc tgccagtgag taggctctgt
     1501 tctcatcaca tccaagaatt tgtcttgggc tctgtggccc acaggcagtc gaagggatgg
     1561 gccatctaac accccttcag aagttttccc acagcacctg tggagcatag ccctctcacc
     1621 agttaaaggc tgctggtttg ctttctttaa tcttcaactt attattatta ttgtgtctag
     1681 caaattgaac tggtaatctt atgacagcac agtttaattg actttatcag ggaaagttgt
     1741 atgttttttt actttttgca ttatgcagta tgcatagtga agcaaatagt tctactttgg
     1801 atttgagtgc atatgaaatt gaattgattg tcaccaacag taaatgaaag atcacatgtt
     1861 atgtctgttg attgcatcct gagctatggc ttccaaagaa aagtaggggt ttttctcatg
     1921 taaaatgggt tttaaaaaat tttcatcctt tcaatattca ctaactttac cctggcaggg
     1981 tgatgcacgc ctttaatccc agcacttcaa aggcagagat gagcagatct ctgtgagttt
     2041 aaggccagcc tggcctacat agttccagga caggcaggga tacatggtga aactatttcc
     2101 aaaaaaaagt gtttggttca ttaggtagcc ttggtatatt tataaaacct tcaccctctg
     2161 gccttagtgt tttaaagccc aagtaccagt ttccttcctt tatatctcag atggcattaa
     2221 acaaacatgc caacacgtaa acctaagtaa gagaacctta gttgtctttt gattataggc
     2281 gtggatcagg gtctagagca catcaactga gtttcccagt ttcttgtgtt ctggaacaag
     2341 aactggacca gggacacatg gatggtgaag aaagaaagga agaaaagaaa aatacccccc
     2401 agaatgagag tgtgcccaag gactaggaaa gtctaagtct gaaaagcctt ggaccaaggt
     2461 ctctgctctt tataggacct ccatcttttc tagcaagtag ggaaggcagg gctttgtttt
     2521 taaagcatgc agtttagtta tgcatagatc tgttggagag gtttccagtc ttcctttggg
     2581 attggttttt gtttatttgc ctctagaatt tcctattctt ctacctcact ttaccccatc
     2641 ctcagagctg tggttccaaa aatctctttg aggcgctaaa ggatgataat ctgttttgta
     2701 taatttccat gttaatgagg gatgtagtct ttgccaaaga gagaccaaag ctcgactcct
     2761 attttatcca gtggttggag aggaggcttt ggagtttttg ttttgggagt cggcttaaat
     2821 ccttgtatca taatgataaa ttgtacatgg tttcttaaaa atgttaagaa tttgggcatg
     2881 gtttagcatt taagagcacc aattgcgggg ttggggattt agctcagtgc tagagcactt
     2941 gcctagcaag cgcaagccct gggttcggtc cccagctccg aaaaaaaaag aaaaaaaaaa
     3001 aagaaaagga aaaagaaaaa aaagagcacc aattgccctt gcaggaacct tgagtctgat
     3061 tcttagcacc tacatggtaa ctcacaacca tccgaaactg tagcttctaa ggatgtcaca
     3121 actttttctg acatacgcag gcaccagaca tgcacacgat acacatacag atgtaagaaa
     3181 aacatccata tacctaaaat aaataaattt ttttaaaaat tgtcaatgca agaataatat
     3241 gattatttca accattagtc ccagaaagga ccagagtcag gcaacccata gctatatatg
     3301 cttatgtaga tatggtcctt tagactgtta aaacaagaac ccaaacaaaa caaaatccac
     3361 aaacaaaaac caaactgatt gtggcaaaat acaaaaagaa caatttgtag aaaaaaatgc
     3421 agtactggaa tctattaagg agaaggaagt gtgggattgt gaccatagag taccaacggt
     3481 gccaaaagaa aaaatggaaa accaaagcat acggttataa gtactgattg ttaatttgcc
     3541 tttaagaagt taaagcatcc tgttattgtc taaagagatc ctttaaatcc ttcagccttt
     3601 taatgtaggt agcctgtctt taagatattc ttgaggctat ggacttgggt ctgtggacac
     3661 tggtgcacct ccttagaagg acctccaaca ggttatatcc tgtctccaaa cagctagtgc
     3721 ccttctttag ccaactagta tcctgtcaga acagtatatt gtctctgccc aggatctcag
     3781 agaaataaga ttttagcaat gtgctaagtt gtaaccaaaa taaggaagac ttttcctttt
     3841 tggtaagact tcatagtaag tcctttttta ttacatacag agtaagtata accagtttga
     3901 tgttactgct tgatggtatt gtaaattcca taattttcat tatggaaaag aagttcaact
     3961 taaagatctt tggcaaatat aaccaccatt ctagatgtat ctcggcttta ccagaaatct
     4021 tatctccatc acttgtacac aggaacacta ctataaaagg aatcagccca acagaagaat
     4081 tcttaacaca gcattcagag cactggatca acataaagca ttttgatgct gttttatttt
     4141 tgttaccctt gactttgaaa aaatgaaata aaattttaaa tctttcacag aa
//
