LOCUS       NM_001034006            3579 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus ArfGAP with coiled-coil, ankyrin repeat and PH
            domains 2 (Acap2), mRNA.
ACCESSION   NM_001034006 XM_001073652
VERSION     NM_001034006.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Etoh,K. and Fukuda,M.
  TITLE     Structure-function analyses of the small GTPase Rab35 and its
            effector protein centaurin-beta2/ACAP2 during neurite outgrowth of
            PC12 cells
  JOURNAL   J Biol Chem 290 (14), 9064-9074 (2015)
   PUBMED   25694427
  REMARK    GeneRIF: showed by knockdown-rescue approaches that neither a
            centaurin-beta2 binding-deficient Rab35(T76S/T81A) mutant nor a
            Rab35 binding-deficient centaurin-beta2(N610A/N691A) mutant
            supported neurite outgrowth of PC12 cells
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Miyamoto,Y., Yamamori,N., Torii,T., Tanoue,A. and Yamauchi,J.
  TITLE     Rab35, acting through ACAP2 switching off Arf6, negatively
            regulates oligodendrocyte differentiation and myelination
  JOURNAL   Mol Biol Cell 25 (9), 1532-1542 (2014)
   PUBMED   24600047
  REMARK    GeneRIF: Rab35/ACAP2 and cytohesin-2 antagonistically control
            oligodendrocyte differentiation and myelination through Arf6
            regulation.
REFERENCE   3  (bases 1 to 3579)
  AUTHORS   Kobayashi,H. and Fukuda,M.
  TITLE     Rab35 establishes the EHD1-association site by coordinating two
            distinct effectors during neurite outgrowth
  JOURNAL   J Cell Sci 126 (Pt 11), 2424-2435 (2013)
   PUBMED   23572513
REFERENCE   4  (bases 1 to 3579)
  AUTHORS   Kobayashi,H. and Fukuda,M.
  TITLE     Rab35 regulates Arf6 activity through centaurin-beta2 (ACAP2)
            during neurite outgrowth
  JOURNAL   J Cell Sci 125 (Pt 9), 2235-2243 (2012)
   PUBMED   22344257
  REMARK    GeneRIF: Rab35 regulates Arf6 activity through centaurin-beta2
            (ACAP2) during neurite outgrowth.
REFERENCE   5  (bases 1 to 3579)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 3579)
  AUTHORS   Jackson,T.R., Brown,F.D., Nie,Z., Miura,K., Foroni,L., Sun,J.,
            Hsu,V.W., Donaldson,J.G. and Randazzo,P.A.
  TITLE     ACAPs are arf6 GTPase-activating proteins that function in the cell
            periphery
  JOURNAL   J Cell Biol 151 (3), 627-638 (2000)
   PUBMED   11062263
REFERENCE   7  (bases 1 to 3579)
  AUTHORS   Dowler,S., Currie,R.A., Campbell,D.G., Deak,M., Kular,G.,
            Downes,C.P. and Alessi,D.R.
  TITLE     Identification of pleckstrin-homology-domain-containing proteins
            with novel phosphoinositide-binding specificities
  JOURNAL   Biochem J 351 (Pt 1), 19-31 (2000)
   PUBMED   11001876
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000011.1.
            
            On Jun 12, 2015 this sequence version replaced NM_001034006.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC090073.1, SRR26643291.31392.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-338               JAXUCZ010000011.1  83043503-83043840
            339-396             JAXUCZ010000011.1  83091476-83091533
            397-516             JAXUCZ010000011.1  83094313-83094432
            517-570             JAXUCZ010000011.1  83095459-83095512
            571-629             JAXUCZ010000011.1  83106612-83106670
            630-813             JAXUCZ010000011.1  83108920-83109103
            814-858             JAXUCZ010000011.1  83113794-83113838
            859-954             JAXUCZ010000011.1  83117009-83117104
            955-1029            JAXUCZ010000011.1  83119270-83119344
            1030-1142           JAXUCZ010000011.1  83123036-83123148
            1143-1194           JAXUCZ010000011.1  83131014-83131065
            1195-1295           JAXUCZ010000011.1  83133425-83133525
            1296-1401           JAXUCZ010000011.1  83134663-83134768
            1402-1610           JAXUCZ010000011.1  83139901-83140109
            1611-1680           JAXUCZ010000011.1  83140620-83140689
            1681-1772           JAXUCZ010000011.1  83142031-83142122
            1773-1933           JAXUCZ010000011.1  83142919-83143079
            1934-2026           JAXUCZ010000011.1  83143960-83144052
            2027-2214           JAXUCZ010000011.1  83145435-83145622
            2215-2324           JAXUCZ010000011.1  83146385-83146494
            2325-2435           JAXUCZ010000011.1  83147681-83147791
            2436-2497           JAXUCZ010000011.1  83149740-83149801
            2498-3579           JAXUCZ010000011.1  83155062-83156143
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="11"
                     /map="11q22"
     gene            1..3579
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /note="ArfGAP with coiled-coil, ankyrin repeat and PH
                     domains 2"
                     /db_xref="GeneID:619382"
                     /db_xref="RGD:1562939"
     exon            1..338
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     CDS             286..2598
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /note="centaurin-beta-2"
                     /codon_start=1
                     /product="arf-GAP with coiled-coil, ANK repeat and PH
                     domain-containing protein 2"
                     /protein_id="NP_001029178.1"
                     /db_xref="GeneID:619382"
                     /db_xref="RGD:1562939"
                     /translation="MKMTVDFEECLKDSPRFRAALEEVEGDVAELELKLDKLVKLCIA
                     MIDTGKAFCAANKQFMNGIRDLAQYSSNDAVVETSLTKFSDSLQEMINFHTILFDQTQ
                     RSIKAQLQNFVKEDLRKFKDAKKQFEKVSEEKENALVKNAQVQRNKQHEVEEAANILT
                     ATRKCFRHIALDYVLQINVLQSKRRSEILKSMLSFMYAHLAFFHQGYDLFSELGPYMK
                     DLGAQLDRLVVDAAKEKREMEQKHSTIQQKDFSSDDSKLEYNVDAANGIVMEGYLFKR
                     ASNAFKTWNRRWFSIQNNQLVYQKKFKDSPTVVVEDLRLCTVKHCEDIERRFCFEVVS
                     PTKSCMLQADSEKLRQAWIKAVQTSIATAYREKGDESEKLDKKSSPSTGSLDSGSESK
                     EKLLKGESALQRVQCIPGNTSCCDCGLADPRWASINLGITLCIECSGIHRSLGVHFSK
                     VRSLTLDTWEPELLKLMCELGNDVINRVYEAKLEKMGVKKPQPGQRQEKEAYIRAKYV
                     ERKFVDKYSTLLSPSEQEKRIISKSCEDQRLSHTRVSVHTPVKSNDSGIQQCSDDGRE
                     SLPSTVSANSLYEPEGERQESSVFLDSKHLNPGLQLYRASYEKNLPKMAEALAHGADV
                     NWANSDENQATPLIQAVLGGSLVTCEFLLQNGANVNQRDVQGRGPLHHATVLGHTGQV
                     CLFLKRGANQHATDEEGKDPLSIAVEAANADIVTLLRLARMNEEMRESEGLYGQPGDE
                     TYQDIFRDFSQMASNNPEKLNRFQQDSQKF"
     exon            339..396
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            397..516
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            517..570
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            571..629
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            630..813
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            814..858
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            859..954
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1029
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1142
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1194
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1295
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1401
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1610
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1680
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1772
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1933
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2026
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2214
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2324
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2435
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            2436..2497
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
     exon            2498..3579
                     /gene="Acap2"
                     /gene_synonym="Centb2; cnt-b2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggggccgcgg ctcggtggct ggctgaccgc ggctgtcact cccgggcgtt ttccccgggc
       61 cggtgctcag ctggggcgga ggaagaggcg gccagggcct gccgagccgt tgcagtcttc
      121 gcggctgcta tgctcaggga gcgcagcgcc ctccgcagct ccgcgcagcc ctagaggcag
      181 ccgagcgccc gccgacgccg cccggatcac cggtgccacc gctaccccgg tcccgcagct
      241 cccgtggatt ctccggcgct gcttgcgagg ccagacggag gcaagatgaa gatgacggtg
      301 gatttcgagg agtgtctgaa ggactcgccc cgtttcaggg ctgctttgga agaagtagaa
      361 ggagatgtgg ctgagctgga actgaaactg gacaagctgg taaaactttg tatcgcgatg
      421 attgataccg gcaaagcctt ttgtgccgca aataaacagt tcatgaatgg gatccgagac
      481 ctggcccagt attctagcaa tgacgctgtg gttgagacga gtttgaccaa gttttctgac
      541 agtcttcagg aaatgatcaa tttccacaca attctgtttg accaaactca gagatcaatt
      601 aaggcccagc ttcagaactt tgtcaaagaa gatcttagaa aattcaaaga tgccaagaaa
      661 caatttgaaa aagtcagtga agaaaaggaa aacgctctag taaaaaacgc ccaagttcaa
      721 agaaacaagc agcacgaggt tgaagaggct gcaaacattc tcacggccac gcggaagtgc
      781 ttccggcaca tagcgctcga ctatgtcctg cagattaacg tgcttcaatc aaaaaggaga
      841 tcggaaatcc tgaaatcaat gctgtccttc atgtatgcac atctggcctt cttccatcaa
      901 gggtatgacc tgttcagtga gcttgggccc tacatgaaag atcttggcgc acagttggat
      961 cgactggttg tggatgcagc aaaggagaaa agagaaatgg agcaaaaaca ttctacgatc
     1021 cagcagaagg atttctccag tgatgattcc aagttagagt ataacgtaga cgctgcaaac
     1081 ggcattgtca tggaagggta tctgttcaaa cgagccagca acgccttcaa aacgtggaac
     1141 agacgctggt tctccataca gaacaatcag ctggtttacc agaaaaagtt taaggacagc
     1201 cccactgtgg tggtggaaga cctcaggctc tgcacagtga agcactgtga agacatcgag
     1261 cgacggttct gctttgaggt cgtctctccc acaaaaagtt gcatgctcca agcagattcc
     1321 gaaaagcttc gccaggcctg gattaaggct gttcagacca gtattgcaac tgcttacaga
     1381 gagaaaggcg atgaatcaga gaaactggat aagaaatcgt ctccatcgac aggaagccta
     1441 gattctggaa gtgagtctaa agagaaatta ctgaaaggag aaagtgctct gcagcgtgtg
     1501 cagtgtatcc ctggcaacac cagctgctgc gactgtggtc tcgcagaccc ccgctgggcc
     1561 agcatcaacc tgggcattac cttgtgtatt gagtgctctg ggattcatcg gagtctcggg
     1621 gttcattttt cgaaagtgag atctttaact ttagacactt gggagcctga gctcttaaag
     1681 cttatgtgtg aactggggaa cgatgttata aatcgtgttt atgaagctaa actggaaaaa
     1741 atgggagtaa agaaaccaca gccaggacaa agacaggaga aggaggcata tatcagagca
     1801 aaatatgtgg agaggaaatt cgtggataaa tactctacat tgctgtcacc ttccgagcag
     1861 gagaaaagga tcatctccaa gagctgtgag gaccagagac tgagccacac cagagtgtct
     1921 gtccacaccc cagtcaaaag taatgacagt gggatccagc aatgctctga tgatggacgg
     1981 gagtctttac cttccaccgt gtcagccaat agcttatacg agcccgaagg agaaaggcaa
     2041 gaatcttctg tgtttctcga ctctaaacat cttaatccag gacttcagct ttatagggct
     2101 tcgtatgaaa aaaaccttcc caaaatggcc gaggctttgg ctcatggtgc agatgtgaac
     2161 tgggctaatt cagatgaaaa ccaagcaaca ccactcattc aggctgtatt agggggctct
     2221 ttggtgacat gtgagttcct gttgcagaat ggtgctaatg tgaaccaaag agatgtccaa
     2281 gggcggggac cgctgcacca cgccaccgtc ttaggacaca cagggcaggt atgtttattc
     2341 ctaaagcgag gtgccaatca acatgccact gatgaagagg ggaaggaccc tctgagtata
     2401 gctgtggaag cagccaatgc tgacatagta acgctgttac gcttagcaag aatgaatgag
     2461 gaaatgcggg aatccgaagg actttatgga cagccaggtg atgaaactta ccaggatatc
     2521 ttccgtgatt tttctcaaat ggcatccaat aatccagaga aactcaatcg tttccagcaa
     2581 gattcacaga agttctgatc cttttaaaag ggggaaacta tgaaatttgg tgaattctta
     2641 atgtgtgcaa ccagaaactc acacattttt tactacttta attccactta gttttggtag
     2701 gcattgaatc gtttggaaaa aaatggtacc cattcatttt ggatttttgt aaatgaaaac
     2761 acgatctctc ataactggga aagggagaaa tccactgtag ttcctctttg atttgagaag
     2821 agaaaagcct tttttttttt tttttaaact gggttgcgct tttatataaa gttgtatcca
     2881 gatctaagca tctctaagtt ctgaggtgct acactggaga cccctccatc ctttgccatg
     2941 catggtggta ctctggttcc cctgggaaca ttcagtcatt gtgtctgcgt caggtggaac
     3001 cagttatctc acctgcttgg aggatgtgct gtcctcataa tcgttggtgg ttcagaagta
     3061 ggtctgcgtg ctttctggta gcttagccgt ggcacccaca gtgtgcagtg cagttgccat
     3121 gaccagtggg ttcgcaccgt gtttgttcag aggtgtcatt gtcctccagg ccccagcaac
     3181 acggttgccg cgtgagtaga ccacacttca ctctttagat ttattttgtc tcatagtgaa
     3241 gagaagcttt tactgttttc tttcttccat cacccaaaga atcctagctc agcttctgtt
     3301 gtcaggttga cttcctgaaa ccttgacaga tcatcagtgc aacaaaaccg gatttcagaa
     3361 attgactatt gcaaaatcgg gctttcatcc aaacacggtg atgtcaaaga taatatttta
     3421 gggcattttt atatttggaa ttaatccatt ttaagataca tattttttag tgttactctt
     3481 ggtgaaagaa aaatctccat gtaatggaag agaaagctaa tttttttcct gtttatacta
     3541 aaacttacca gcaataaagt ccttttattt ttttctaca
//
