LOCUS       NM_022190               7728 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus aggrecan (Acan), mRNA.
ACCESSION   NM_022190 XM_039101034
VERSION     NM_022190.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Wu,Y., Xia,Y., Yue,C., Xin,T., Wang,Q., Zhang,H., Shen,C., Shen,M.,
            Gu,Y. and Shen,J.
  TITLE     Brachyury positively regulates extracellular matrix synthesis via
            directly promoting aggrecan transcription in nucleus pulposus
  JOURNAL   FASEB J 37 (6), e22976 (2023)
   PUBMED   37227215
  REMARK    GeneRIF: Brachyury positively regulates extracellular matrix
            synthesis via directly promoting aggrecan transcription in nucleus
            pulposus.
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Nham,G.T.H., Zhang,X., Asou,Y. and Shinomura,T.
  TITLE     Expression of type II collagen and aggrecan genes is regulated
            through distinct epigenetic modifications of their multiple
            enhancer elements
  JOURNAL   Gene 704, 134-141 (2019)
   PUBMED   30981839
  REMARK    GeneRIF: E1 and E2 enhancer activities in type II collagen and
            aggrecan are regulated through distinct epigenetic modifications by
            histone deacetylase 10 and sirtuin 6.
REFERENCE   3  (bases 1 to 7728)
  AUTHORS   Dzyubenko,E., Rozenberg,A., Hermann,D.M. and Faissner,A.
  TITLE     Colocalization of synapse marker proteins evaluated by
            STED-microscopy reveals patterns of neuronal synapse distribution
            in vitro
  JOURNAL   J Neurosci Methods 273, 149-159 (2016)
   PUBMED   27615741
REFERENCE   4  (bases 1 to 7728)
  AUTHORS   Barallobre-Barreiro,J., Oklu,R., Lynch,M., Fava,M., Baig,F.,
            Yin,X., Barwari,T., Potier,D.N., Albadawi,H., Jahangiri,M.,
            Porter,K.E., Watkins,M.T., Misra,S., Stoughton,J. and Mayr,M.
  TITLE     Extracellular matrix remodelling in response to venous
            hypertension: proteomics of human varicose veins
  JOURNAL   Cardiovasc Res 110 (3), 419-430 (2016)
   PUBMED   27068509
REFERENCE   5  (bases 1 to 7728)
  AUTHORS   Zhu,Y., Tao,H., Jin,C., Liu,Y., Lu,X., Hu,X. and Wang,X.
  TITLE     Transforming growth factor-beta1 induces type II collagen and
            aggrecan expression via activation of extracellular
            signal-regulated kinase 1/2 and Smad2/3 signaling pathways
  JOURNAL   Mol Med Rep 12 (4), 5573-5579 (2015)
   PUBMED   26165845
  REMARK    GeneRIF: The ERK1/2 and Smad2/3 signaling pathways regulate type II
            collagen and aggrecan expression in rat chondrocytes.
REFERENCE   6  (bases 1 to 7728)
  AUTHORS   Watanabe,H., Kimata,K., Line,S., Strong,D., Gao,L.Y., Kozak,C.A.
            and Yamada,Y.
  TITLE     Mouse cartilage matrix deficiency (cmd) caused by a 7 bp deletion
            in the aggrecan gene
  JOURNAL   Nat Genet 7 (2), 154-157 (1994)
   PUBMED   7920633
REFERENCE   7  (bases 1 to 7728)
  AUTHORS   Seegmiller,R.E., Brown,K. and Chandrasekhar,S.
  TITLE     Histochemical, immunofluorescence, and ultrastructural differences
            in fetal cartilage among three genetically distinct
            chondrodystrophic mice
  JOURNAL   Teratology 38 (6), 579-592 (1988)
   PUBMED   3070812
REFERENCE   8  (bases 1 to 7728)
  AUTHORS   Neame,P.J., Christner,J.E. and Baker,J.R.
  TITLE     Cartilage proteoglycan aggregates. The link protein and
            proteoglycan amino-terminal globular domains have similar
            structures
  JOURNAL   J Biol Chem 262 (36), 17768-17778 (1987)
   PUBMED   3693371
REFERENCE   9  (bases 1 to 7728)
  AUTHORS   Doege,K., Sasaki,M., Horigan,E., Hassell,J.R. and Yamada,Y.
  TITLE     Complete primary structure of the rat cartilage proteoglycan core
            protein deduced from cDNA clones
  JOURNAL   J Biol Chem 262 (36), 17757-17767 (1987)
   PUBMED   3693370
  REMARK    Erratum:[J Biol Chem 1988 Jul 15;263(20):10040]
REFERENCE   10 (bases 1 to 7728)
  AUTHORS   Doege,K., Fernandez,P., Hassell,J.R., Sasaki,M. and Yamada,Y.
  TITLE     Partial cDNA sequence encoding a globular domain at the C terminus
            of the rat cartilage proteoglycan
  JOURNAL   J Biol Chem 261 (18), 8108-8111 (1986)
   PUBMED   2424893
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On or before Mar 16, 2022 this sequence version replaced
            XM_039101034.1, NM_022190.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J03485.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN11044071, SAMN12840109
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-381               JAXUCZ010000001.1  142390951-142391331
            382-458             JAXUCZ010000001.1  142420594-142420670
            459-842             JAXUCZ010000001.1  142422979-142423362
            843-1017            JAXUCZ010000001.1  142424745-142424919
            1018-1145           JAXUCZ010000001.1  142426726-142426853
            1146-1439           JAXUCZ010000001.1  142428195-142428488
            1440-1844           JAXUCZ010000001.1  142429538-142429942
            1845-2019           JAXUCZ010000001.1  142430904-142431078
            2020-2147           JAXUCZ010000001.1  142431521-142431648
            2148-2441           JAXUCZ010000001.1  142432812-142433105
            2442-2645           JAXUCZ010000001.1  142435585-142435788
            2646-6116           JAXUCZ010000001.1  142436826-142440296
            6117-6230           JAXUCZ010000001.1  142446813-142446926
            6231-6389           JAXUCZ010000001.1  142449889-142450047
            6390-6472           JAXUCZ010000001.1  142450557-142450639
            6473-6617           JAXUCZ010000001.1  142451248-142451392
            6618-6800           JAXUCZ010000001.1  142452195-142452377
            6801-7728           JAXUCZ010000001.1  142452852-142453779
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q31"
     gene            1..7728
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /note="aggrecan"
                     /db_xref="GeneID:58968"
                     /db_xref="RGD:68358"
     exon            1..381
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            382..458
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             389..6877
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /note="aggrecan core protein; CSPCP; cartilage-specific
                     proteoglycan core protein; aggrecan 1; aggrecan,
                     structural proteoglycan of cartilage"
                     /codon_start=1
                     /product="aggrecan core protein precursor"
                     /protein_id="NP_071526.2"
                     /db_xref="GeneID:58968"
                     /db_xref="RGD:68358"
                     /translation="MTTLLLVFVTLRVIAAVISEEVPDHDNSLSVSIPQPSPLKALLG
                     TSLTIPCYFIDPMHPVTTAPSTAPLTPRIKWSRVSKEKEVVLLVATEGQVRVNSIYQD
                     KVSLPNYPAIPSDATLEIQNLRSNDSGIYRCEVMHGIEDSEATLEVIVKGIVFHYRAI
                     STRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPRE
                     GCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRL
                     GARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQ
                     TGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITIQTVTWPDLELPLPRNITEGE
                     ARGNVILTAKPIFDMSPTVSEPGEALTLAPEVGTTVFPEAGERTEKTTRPWGFPEEAT
                     RGPDSATAFASEDLVVRVTISPGAVEVPGQPRLPGGVVFHYRPGSTRYSLTFEEAQQA
                     CIRTGAAIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRT
                     YGVRPSSETYDVYCYVDKLEGEVFFATQMEQFTFQEAQAFCAAQNATLASTGQLYAAW
                     SQGLDKCYAGWLADGTLRYPIVNPRPACGGDKPGVRTVYLYPNQTGLPDPLSKHHAFC
                     FRGVSVAPSPGGTPTSPSDIEDWIVTRVEPGVDAVPLEPETTEVPYFTTEPEKQTEWE
                     PAYTPVGTSPLPGIPPTWLPTVPAAEEHTESPSASQEPSASQVPSTSEEPYTPSLAVP
                     SGTELPSSGDTSGAPDLSGDFTGSTDTSGRLDSSGEPSGGSESGLPSGDLDSSGLGPT
                     VSSGLPVESGSASGDGEIPWSSTPTVGRLPSGGESLEGSASASGTGDLSGLPSGGEIT
                     ETSASGTEEISGLPSGGDDLETSTSGIDGASVLPTGRGGLETSASGVEDLSGLPSGEE
                     GSETSTSGIEDISVLPTGESPETSASGVGDLSGLPSGGESLETSASGVEDVTQLPTER
                     GGLETSASGIEDITVLPTGRENLETSASGVEDVSGLPSGKEGLETSASGIEDISVFPT
                     EAEGLETSASGGYVSGIPSGEDGTETSTSGVEGVSGLPSGGEGLETSASGVEDLGLPT
                     RDSLETSASGVDVTGYPSGREDTETSVPGVGDDLSGLPSGQEGLETSASGAEDLGGLP
                     SGKEDLVGSASGALDFGKLPSGTLGSGQTPEASGLPSGFSGEYSGVDIGSGPSSGLPD
                     FSGLPSGFPTVSLVDSTLVEVITATTASELEGRGTISVSGSGEESGPPLSELDSSADI
                     SGLPSGTELSGQTSGSLDVSGETSGFFDVSGQPFGSSGTGEGTSGIPEVSGQPSGSPD
                     TTEISELSGLSSGQPDVSGEGSGILFGSGQSSGITSVSGETSGISDLSGQPSGFPVLS
                     GTTPGTPDLASGAMSGSGDSSGITFVDTSLIEVTPTTFREEEGLGSVELSGLPSGETD
                     LSGTSGMVDVSGQSSGAIDSSGLISPTPEFSGLPSGVAEVSGEVSGVETGSSLSSGAF
                     DGSGLVSGFPTVSLVDRTLVESITLAPTAQEAGEGPSSILEFSGAHSGTPDISGDLSG
                     SLDQSTWQPGWTEASTEPPSSPYFSGDFSSTTDASGESITAPTGSGETSGLPEVTLIT
                     SELVEGVTEPTVSQELGHGPSMTYTPRLFEASGEASASGDLGGPVTIFPGSGVEASVP
                     EGSSDPSAYPEAGVGVSAAPEASSQLSEFPDLHGITSASRETDLEMTTPGTEVSSNPW
                     TFQEGTREGSAAPEVSGESSTTSDIDAGTSGVPFATPMTSGDRTEISGEWSDHTSEVN
                     VTVSTTVPESRWAQSTQHPTETLQEIGSPNPSYSGEETQTAETAKSLTDTPTLASPEG
                     SGETESTAADIDECLSSPCLNGATCVDALDTFTCLCLPSYRGDLCEIDQEQCEEGWTK
                     FQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNKNAQDYQWIGLNDRTI
                     EGDFRWSDGHSLQFEKWRPNQPDNFFATGEDCVVMIWHERGEWNDVPCNYQLPFTCKK
                     GTVACGEPPAVEHARTLGQKKDRYEISSLVRYQCTEGFVQRHVPTIRCQPSGHWEEPR
                     ITCTDPNTYKHRLQKRSMRPTRRSRPSMAH"
     exon            459..842
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1017
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1145
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1439
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1844
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..2019
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2147
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2441
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2645
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2646..6116
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6117..6230
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6231..6389
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6390..6472
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6473..6617
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6618..6800
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
     exon            6801..7728
                     /gene="Acan"
                     /gene_synonym="Agc; Agc1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acctacctcc ccgcggtgcc agaggggctc acagagctga ggacgcgtgc agtaccgccc
       61 aaggtccctc attctccgcg gctagcatct gacgcaggtg ccgagggaac tcggggcacc
      121 tttctgtgtc ccttccctcc atcgaccctg ctccgaaacc cagccagcca gctaccactg
      181 cggccaccgc ccgaggggac ttgcggagga gacgccggcg ggaggagggc ttcgcagccc
      241 ccacccccac ccccactccc agcccagagc atccctgctg cagcgcagca agacccgggg
      301 ctgccagccc ccaggaatcc ctagctgctt agcagggata acggactgaa gttcttggag
      361 gagcgagttc aactcttcaa gctgaactat gaccacttta ctcttggtct ttgtgactct
      421 gcgggtcatc gctgcagtga tctcagaaga agttccagac catgacaact cactgagtgt
      481 gagcatccct caaccatccc cactgaaggc cctcctaggg acttccctca ccatcccctg
      541 ctacttcatc gaccccatgc atcctgtgac cactgctccc tccactgccc ccctcacccc
      601 aagaatcaag tggagccgtg tttccaagga aaaggaggtg gtactgttgg tggccactga
      661 aggacaggtt cgagtgaaca gcatctacca agacaaggtg tcacttccca actatccagc
      721 catccccagc gatgccacct tggaaatcca gaaccttcgc tccaatgact ctgggatcta
      781 tcggtgtgaa gtgatgcatg gcattgagga cagcgaagcc accctggagg tcatagtaaa
      841 aggcattgtg ttccactaca gagctatttc cacacgctac acactggact ttgaccgagc
      901 ccagcgggct tgccttcaga acagcgccat catcgccacc cctgagcaac tgcaggccgc
      961 ctatgaggat ggcttccacc agtgtgatgc aggctggctg gctgatcaga cagtcagata
     1021 ccccatccac actccccggg aaggttgcta tggtgacaag gacgagttcc ctggagtgag
     1081 gacctatgga atccgagaca ccaacgagac ctatgatgtg tactgcttcg ctgaagagat
     1141 ggagggtgag gtcttttatg ccacatcccc ggagaaattc accttccagg aggcagccaa
     1201 cgagtgccgg aggctggggg cacggttggc caccacaggc cagctctacc ttgcctggca
     1261 gggcggtatg gacatgtgca gcgctggctg gctggcggac cgcagcgttc gctaccccat
     1321 ctccaaggct cggcccaact gcggaggcaa cctcctgggt gtaaggactg tctatctgca
     1381 cgccaaccag acaggctacc ctgatccctc atcccgctac gacgccatct gctacacagg
     1441 tgaagacttt gtagacatcc cagaaaactt cttcggagtg ggtggtgaag aggacatcac
     1501 catccagaca gtgacctggc cagatctgga gctgcccctg ccccgtaata ttacggaggg
     1561 agaagcccgg ggcaatgtga tcctcactgc aaagcccatc ttcgacatgt cccccactgt
     1621 ctcagagcct ggggaggccc tcacacttgc ccctgaagtg gggaccacag tcttccctga
     1681 ggctggggag agaactgaaa agaccaccag gccctggggc tttcccgagg aagccacacg
     1741 tgggcctgat tctgccactg ccttcgccag tgaggacctg gtggtgcgag tgaccatctc
     1801 tccaggtgca gttgaggtcc ctggtcagcc ccgcttgcca gggggagttg tattccacta
     1861 ccgcccgggc tctaccagat actctctgac atttgaggag gcacagcagg cctgcattcg
     1921 cacgggagca gccatagctt ctcctgagca actccaggct gcctatgagg caggctatga
     1981 gcagtgtgat gctggctggc tgcaggacca gactgtcaga taccccattg tgagcccacg
     2041 gaccccatgt gtgggtgaca aggacagcag ccccggagtc aggacctatg gcgtgcgccc
     2101 atcatcagaa acctatgatg tctactgcta cgtggacaag cttgaggggg aagtgttctt
     2161 cgccacacaa atggagcagt tcaccttcca ggaagctcag gccttctgtg cagctcaaaa
     2221 tgctaccctg gcctccactg gccagctcta cgctgcctgg agccagggcc tggacaagtg
     2281 ctatgctggc tggttggcag atggcaccct ccgatacccc atcgtcaacc ctcggcctgc
     2341 ctgtggtggt gacaagccgg gcgtgagaac tgtctacctc taccccaacc agaccggcct
     2401 ccctgatcca ctgtccaagc accatgcctt ctgcttccga ggtgtgtcag tggcgccctc
     2461 tccaggtggt acacccacat cgccctctga catagaggac tggatcgtca ctcgagtgga
     2521 gcctggcgtg gatgctgtcc ccttggagcc ggagacgaca gaagtaccat atttcaccac
     2581 tgagccagaa aaacagactg aatgggagcc agcctacacc ccagtgggca catccccatt
     2641 gccagggatc cctcccacat ggcttcccac cgtcccagca gcagaggaac acaccgaaag
     2701 tccctctgcc tctcaagagc cctctgcctc gcaagtccct tccacatcag aagagccata
     2761 cacaccttca cttgcagtgc caagcgggac tgagctgccc agctcagggg acacatcagg
     2821 ggcacctgac ctcagtggtg acttcacagg cagcacagac acttcaggac gccttgactc
     2881 cagtggggag ccatcaggag gcagtgaaag tggacttccc tctggcgacc ttgactccag
     2941 tggtctcggc cccacagtga gctcaggctt gcctgtagaa agtgggtcag cctcgggaga
     3001 tggagaaatc ccctggtcga gcactcccac agttggcaga ctgccctctg gaggtgagag
     3061 ccttgaaggt tctgcctctg cctctggaac aggagacctt agtgggctgc cttcaggagg
     3121 ggaaattaca gaaacttctg cttctgggac agaagaaatc agtggacttc cttctggagg
     3181 agacgatcta gaaacttcta cctctggaat agatggagcc agtgtgcttc ctactggaag
     3241 aggaggtcta gagacgtctg cctctggagt agaggacctc agtggacttc cttctggaga
     3301 agaaggttca gaaacatcta cctctggaat agaggacatt agtgtacttc cgactggaga
     3361 aagtccagaa acttctgctt ctggagtggg agacttgagt ggacttccct caggaggaga
     3421 aagtctagaa acatctgctt caggtgtaga ggatgtcacc cagcttccta ctgaaagagg
     3481 aggtctagag acttctgcct ctggaataga ggacatcact gtgcttccta ctggaagaga
     3541 aaatctagaa acttctgcct ctggagtaga ggatgtcagt ggacttcctt ctggaaaaga
     3601 aggtctagag acttctgcct ctggaataga ggacattagt gtgttcccta ctgaagcaga
     3661 aggtctagaa acttctgcct ccgggggata tgtcagtggg attccttctg gagaagatgg
     3721 tacagaaacc tctacttctg gagtagaggg tgtgagtggt cttccttctg gaggagaggg
     3781 tctagaaacg tctgcctctg gagtagaaga tcttggtctt cctacaagag atagtctaga
     3841 gacttctgcc tcaggagtag atgttactgg gtatccttct ggaagagagg acacagaaac
     3901 ctctgttcct ggggtaggtg atgaccttag tggacttcct tctggacaag aaggcctaga
     3961 gacctcagct tcaggagctg aggaccttgg tggcttgcct tctggaaaag aagacttggt
     4021 agggtctgct tctggagccc tggacttcgg caagctacct tctggaactc taggaagtgg
     4081 tcaaactcca gaagctagtg gccttccctc tggatttagt ggtgaatatt ctggagtgga
     4141 cattggaagt ggcccatcct ctggcctgcc tgactttagt ggacttccat ccggcttccc
     4201 aacagtctcc cttgtggaca gtaccttagt ggaagtgatc acagccacca ctgccagtga
     4261 actggaagga agggggacca tcagcgtcag tggttcagga gaagagtcag ggccgcccct
     4321 cagtgagttg gacagtagtg cggacattag tggtctccct tcaggaactg aactcagtgg
     4381 ccaaacatcc ggatctcttg atgtcagtgg agaaacatct ggattttttg atgttagtgg
     4441 acagccattt gggtcttctg gcactggtga gggaacatct gggattcctg aagtcagtgg
     4501 acagccgtca gggagtcctg acaccactga gatatctgag cttagtggac tgtcctctgg
     4561 gcaaccagat gtcagtggag aagggtctgg aattctcttt ggcagtggcc aatcctctgg
     4621 cataacatct gtgagtggag aaacctctgg gatttctgat ctcagtgggc aaccttcagg
     4681 gttcccagtg ctcagcggaa caacacctgg aacccctgac ctggcttctg gcgccatgag
     4741 tggcagtgga gattcttctg gcattacgtt tgtggacacc agtcttattg aagtgacccc
     4801 taccacattt agagaagaag aggggttagg gtctgtggaa ctcagtggcc tcccttctgg
     4861 ggagacagat ctgtctggca catctgggat ggtggatgtc agtggacaat cttctggagc
     4921 aatcgattcc agtggactca tatcccccac tccagaattc agtggcctcc caagcggagt
     4981 agctgaggtc agtggagagg tctctggagt tgagactggg agcagcttgt cctcgggagc
     5041 attcgatggc agtggacttg tctcaggttt ccctactgtg tctcttgtag acagaacttt
     5101 ggtggagtct ataaccctgg ctcctactgc tcaagaagct ggagaagggc cttccagcat
     5161 tttggaattc agtggtgccc attctggaac accagacata tctggggacc tctctgggtc
     5221 tttagaccaa agcacatggc agcctgggtg gacagaagcc agcacagagc caccaagctc
     5281 cccatatttt agtggggact tctctagcac cactgatgca agtggagaat ccataactgc
     5341 cccaactggc agtggggaaa cctcggggct tccggaagtc actttaatca cttcagagtt
     5401 agtggagggc gtgactgaac caactgtttc ccaggaactt ggccatggtc cttctatgac
     5461 atatactccc cggctctttg aggccagtgg ggaagcctca gcatctgggg accttggtgg
     5521 acctgtaaca atctttcccg ggtctggggt agaagcttca gtcccagaag gcagcagtga
     5581 cccatctgct taccctgagg ctggagtggg agtgtctgct gcccctgagg ccagcagtca
     5641 attgtctgag ttcccagatc tgcatggaat cacctctgcc tcccgtgaaa cagatctgga
     5701 gatgacaacc ccaggcacgg aggtgagcag caacccatgg acctttcagg aaggcaccag
     5761 ggagggatcc gctgctccag aagtgagtgg agaatctagc actacctccg acatagatgc
     5821 aggcacttca ggtgtgcctt ttgccacgcc catgacttct ggagacagga ctgaaatcag
     5881 tggagaatgg tctgatcata cctcagaggt gaatgttacc gtcagcacca ccgtcccaga
     5941 gtccaggtgg gcccagtcta cccagcaccc tacagagaca cttcaagaaa tcggatcccc
     6001 aaatccctca tactcaggag aagagaccca aacagcagaa acagccaagt ccctgacaga
     6061 cacccctacc cttgcttctc cagaagggtc aggagaaaca gagtcaaccg ctgcagacat
     6121 tgatgagtgc ctctcaagcc cttgtctgaa tggagccacc tgcgtggatg ccttggacac
     6181 tttcacatgc ttatgccttc cgagctacag aggggacctg tgtgagatcg accaggagca
     6241 atgtgaggag gggtggacca agttccaggg ccactgttat cgccactttc ccgaccgcga
     6301 gacctgggtg gatgcagaga gacggtgtcg ggagcagcag tcacatctga gcagcatcgt
     6361 cacccctgag gaacaggagt tcgtcaacaa aaatgctcaa gactaccagt ggatcggtct
     6421 gaatgacaga accatcgaag gggacttccg ctggtctgat ggacactccc tgcaatttga
     6481 gaagtggcgt ccaaaccaac ccgacaattt ctttgccact ggagaggact gcgtagtgat
     6541 gatctggcat gagaggggtg aatggaacga tgtcccctgc aattaccagc tgcccttcac
     6601 atgtaaaaag ggcacagtgg cctgtggaga acccccagcg gtggagcatg ccagaaccct
     6661 tgggcagaag aaagatcgct atgagatcag ctccctggtg cggtaccagt gtactgaggg
     6721 ctttgtccag cgccatgtgc ccaccatccg gtgtcagccc agtgggcact gggaagagcc
     6781 tcgaatcact tgcacagacc ccaacaccta caagcacagg ctacagaagc ggagcatgag
     6841 acccacacgg aggagccgcc ccagcatggc ccactgagag gagcttccac aatgtgccca
     6901 ggatgctgag cccagcggcc agccatgctg accgtgcatc ccactcacat ggtgtcttct
     6961 tgtcgctttt tgtcatataa ggaatccatt aaagaaggaa aaaaaagcca cattgtgtgt
     7021 gccccactcc ccatatcacc caaactgcat ctaatttatc caccaccatc accgcccccc
     7081 ataaaaaacc gccaaagcaa attcactgta acccatggac tgagtttaga gacatttctt
     7141 tcatttccca ccgtgccttt ccagggacca gtgcagggac agggtgagaa gagttaaatt
     7201 aaataaagaa gattattttt tgttgttgct tcctgacttt acccaagaac agcacaattg
     7261 ttggttactt cacctccagg gagagctagg ggccagaggt ggaattggaa ggagacaggg
     7321 ccagggagag aggagtaggg cagccacaca gaccagagct ttaaggactc agaagaggca
     7381 aactatagct tagtgtgttg gacaaaaggg atcgaggtct gtgccatcta tgtgggaagg
     7441 agcctggggc agggggcagg ctggccaaag ggaagtgaag ttgcctttcc tccagggccc
     7501 ctctcctagg aacctttctc ctaggaacct ttcttctggg tcaaccaccc tagcaggtgt
     7561 catttctcgg ctggactggg taggggcact tccttcccag gagtgctccg ccgtcccctt
     7621 ccctctcccc accccgggac ggtgcaggca ccagtgttcc gtgcacctat ttatattttt
     7681 gaaaactaaa gattatgata attataataa taaagacatt ggaagaga
//
