LOCUS       NM_016986               1954 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus acyl-CoA dehydrogenase medium chain (Acadm),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_016986
VERSION     NM_016986.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,C., Zhang,L., Wang,X., He,S., Bai,J., Li,Q., Zhang,M., Zhang,C.,
            Yu,X., Zhang,J., Xin,W., Li,Y. and Zhu,D.
  TITLE     piRNA-63076 contributes to pulmonary arterial smooth muscle cell
            proliferation through acyl-CoA dehydrogenase
  JOURNAL   J Cell Mol Med 24 (9), 5260-5273 (2020)
   PUBMED   32227582
  REMARK    GeneRIF: piRNA-63076 contributes to pulmonary arterial smooth
            muscle cell proliferation through acyl-CoA dehydrogenase.
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Martin,J., Balmer,M.L., Rajendran,S., Maurhofer,O., Dufour,J.F. and
            St-Pierre,M.V.
  TITLE     Nutritional stress exacerbates hepatic steatosis induced by
            deletion of the histidine nucleotide-binding (Hint2) mitochondrial
            protein
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 310 (7), G497-G509 (2016)
   PUBMED   26767982
REFERENCE   3  (bases 1 to 1954)
  AUTHORS   Malecki,J., Ho,A.Y., Moen,A., Dahl,H.A. and Falnes,P.O.
  TITLE     Human METTL20 is a mitochondrial lysine methyltransferase that
            targets the beta subunit of electron transfer flavoprotein
            (ETFbeta) and modulates its activity
  JOURNAL   J Biol Chem 290 (1), 423-434 (2015)
   PUBMED   25416781
REFERENCE   4  (bases 1 to 1954)
  AUTHORS   Kim,J.E., Choi,S.I., Lee,H.R., Hwang,I.S., Lee,Y.J., An,B.S.,
            Lee,S.H., Kim,H.J., Kang,B.C. and Hwang,D.Y.
  TITLE     Selenium significantly inhibits adipocyte hypertrophy and abdominal
            fat accumulation in OLETF rats via induction of fatty acid
            beta-oxidation
  JOURNAL   Biol Trace Elem Res 150 (1-3), 360-370 (2012)
   PUBMED   23076603
REFERENCE   5  (bases 1 to 1954)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   6  (bases 1 to 1954)
  AUTHORS   Inagaki,T., Ohishi,N., Tsukagoshi,N., Udaka,S., Ghisla,S. and
            Yagi,K.
  TITLE     Structurally different rat liver medium-chain acyl CoA
            dehydrogenases directed by complementary DNAs differing in their
            5'-region
  JOURNAL   Biochim Biophys Acta 1077 (3), 285-290 (1991)
   PUBMED   2029527
REFERENCE   7  (bases 1 to 1954)
  AUTHORS   Gregersen,N., Andresen,B.S., Bross,P., Winter,V., Rudiger,N.,
            Engst,S., Christensen,E., Kelly,D., Strauss,A.W., Kolvraa,S. et al.
  TITLE     Molecular characterization of medium-chain acyl-CoA dehydrogenase
            (MCAD) deficiency: identification of a lys329 to glu mutation in
            the MCAD gene, and expression of inactive mutant enzyme protein in
            E. coli
  JOURNAL   Hum Genet 86 (6), 545-551 (1991)
   PUBMED   1902818
REFERENCE   8  (bases 1 to 1954)
  AUTHORS   Matsubara,Y., Narisawa,K., Miyabayashi,S., Tada,K., Coates,P.M.,
            Bachmann,C., Elsas,L.J. 2nd, Pollitt,R.J., Rhead,W.J. and Roe,C.R.
  TITLE     Identification of a common mutation in patients with medium-chain
            acyl-CoA dehydrogenase deficiency
  JOURNAL   Biochem Biophys Res Commun 171 (1), 498-505 (1990)
   PUBMED   2393404
REFERENCE   9  (bases 1 to 1954)
  AUTHORS   Bross,P., Engst,S., Strauss,A.W., Kelly,D.P., Rasched,I. and
            Ghisla,S.
  TITLE     Characterization of wild-type and an active site mutant of human
            medium chain acyl-CoA dehydrogenase after expression in Escherichia
            coli
  JOURNAL   J Biol Chem 265 (13), 7116-7119 (1990)
   PUBMED   1970566
REFERENCE   10 (bases 1 to 1954)
  AUTHORS   McMullin,P.F.
  TITLE     A skeletal growth defect in the puma (Felis concolor)
  JOURNAL   Vet Rec 103 (16), 356-358 (1978)
   PUBMED   734877
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1, CK359511.1, J02791.1 and BP502473.1.
            
            On Mar 30, 2010 this sequence version replaced NM_016986.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: J02791.1, SRR21887622.1144241.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760386,
                                           SAMEA5760400 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                JAXUCZ010000002.1  245542780-245542864 c
            86-818              CK359511.1         1-733
            819-1928            J02791.1           734-1843
            1929-1954           BP502473.1         1-26                c
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q45"
     gene            1..1954
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /note="acyl-CoA dehydrogenase medium chain"
                     /db_xref="GeneID:24158"
                     /db_xref="RGD:2012"
     exon            1..140
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..1376
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /EC_number="1.3.8.7"
                     /note="medium-chain specific acyl-CoA dehydrogenase,
                     mitochondrial; medium-chain acyl-CoA dehydrogenase;
                     acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain;
                     acyl-CoA dehydrogenase, C-4 to C-12 straight chain;
                     acyl-Coenzyme A dehydrogenase, medium chain; Acyl-Coenzyme
                     A dehydrogenase, C-4 to C-12 straight-chain"
                     /codon_start=1
                     /product="medium-chain specific acyl-CoA dehydrogenase,
                     mitochondrial precursor"
                     /protein_id="NP_058682.2"
                     /db_xref="GeneID:24158"
                     /db_xref="RGD:2012"
                     /translation="MAAALRRGYKVLRSVSHFECRAQHTKPSLKQEPGLGFSFELTEQ
                     QKEFQTIARKFAREEIIPVAPDYDKSGEYPFPLIKRAWELGLINTHIPESCGGLGLGT
                     FDACLITEELAYGCTGVQTAIEANSLGQMPVIIAGNDQQKKKYLGRMTEQLMMCAYCV
                     TEPSAGSDVAGIKTKAEKKGDEYVINGQKMWITNGGKANWYFVLTRSNPDPKVPASKA
                     FTGFIVEADTPGIHIGKKELNMGQRCSDTRGITFEDVRVPKENVLIGEGAGFKIAMGA
                     FDRTRPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELAR
                     LSYQRAAWEVDSGRRNTYFASIAKAFAGDIANQLATDAVQIFGGYGFNTEYPVEKLMR
                     DAKIYQIYEGTAQIQRLIIAREHIEKYKN"
     exon            141..228
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            229..326
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            327..396
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            397..497
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            498..578
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            579..709
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            710..818
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            819..959
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1055
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1304
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1953
                     /gene="Acadm"
                     /gene_synonym="MCAD"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgcccctct ccgccctcca ggccacgccc ctgtgggcgg gaccccagtt cgcttagctt
       61 ctgccctgtg gtggtcggag cctgcagagc caacagagca ggaaggcatc atggcagcag
      121 cgctccgcag aggctacaag gtcctgagaa gtgtctctca ttttgagtgt cgagcacaac
      181 acacaaaacc atctctcaag caggagccgg gactagggtt tagcttcgag ttgacggagc
      241 agcagaaaga gtttcaaaca attgctcgga agtttgccag agaggaaata atcccggtcg
      301 ccccagacta cgataaaagc ggggaatacc cgttccctct catcaagaga gcctgggaac
      361 ttgggttgat caacacacac attccggaga gttgtggtgg tcttggcctg ggaacttttg
      421 atgcgtgttt aattacggaa gagttggcat atgggtgtac aggggtgcag actgctattg
      481 aagcaaattc tttggggcaa atgcctgtga ttattgctgg aaatgatcaa cagaagaaga
      541 agtatttggg gaggatgacg gagcagctga tgatgtgtgc ctactgcgtg acagaaccct
      601 cagcaggctc tgatgtggcg ggcattaaga ccaaagcaga gaagaagggt gatgaatatg
      661 tcatcaatgg ccagaagatg tggataacca acgggggaaa ggccaactgg tattttgtat
      721 tgacgcgatc taacccagat cctaaagtac ctgctagtaa agccttcacc ggattcatcg
      781 tggaggccga caccccggga atacacatcg gaaaaaagga actaaacatg ggtcagcggt
      841 gctctgacac cagaggaatc accttcgaag atgtcagagt gcctaaggaa aatgtgttaa
      901 ttggtgaagg agcaggtttc aagattgcaa tgggggcttt tgatagaacc aggccgacgg
      961 tcgcagctgg tgctgtcggg ctagcccaga gagccctgga cgaagctact aagtatgccc
     1021 tggacaggaa aacatttgga aagctgctag tggagcacca aggagtttca tttctgctcg
     1081 cagaaatggc gatgaaagtt gaactggcca gactcagtta ccagcgagca gcctgggagg
     1141 ttgactccgg ccgccggaac acgtactttg cctctattgc gaaggccttt gctggagata
     1201 ttgccaacca gctcgctacc gatgctgtgc agattttcgg aggctatgga ttcaacactg
     1261 agtacccagt agaaaagctg atgcgggacg ccaagatcta tcagatttac gaaggtactg
     1321 cacaaattca gaggctgatc atagctcgtg agcacattga aaagtataaa aattaacaga
     1381 aattactatc gaacgatgct tcaccctcat gtaactacgc tcagagcact gttgctgctt
     1441 cagggggaaa gggctttact tgtcttccca cagaaatgag ataaaagacg cgtgtcacag
     1501 atctgtgcaa tggggtccca cggcggaggg tgcctctgtt gagttccaca gtgacccttt
     1561 ctagataggt ttggttttgg acagtgagtg gtcagtcctt ggccccgaat tgtgttaatt
     1621 tgctccttga tcacttgaga tggagaaata ccctggagtt ctaatgctca ttcaagtgac
     1681 aagaaaggta gcctgtcacg aaagaactca ggattctaca cagacactga ggaatgtggc
     1741 ggattggacc catcacactg tgaagagaga gcatttctgt gctgagctgt ttcataattt
     1801 tgattatatt tcccttgtat tgcagaagag taaaaaagtt tatatgcatt ttctcccatt
     1861 ataaaactaa aaactttctg gaaaatctta attctgaact ggcattttat ttgtcttgat
     1921 tacaatgatt caataaagct agccttaact tata
//
