LOCUS       NM_022193               9030 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus acetyl-CoA carboxylase alpha (Acaca), mRNA.
ACCESSION   NM_022193
VERSION     NM_022193.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Zhang,Y., Gu,Y., Chen,Y., Huang,Z., Li,M., Jiang,W., Chen,J.,
            Rao,W., Luo,S., Chen,Y., Chen,J., Li,L., Jia,Y., Liu,M. and Zhou,F.
  TITLE     Dingxin Recipe IV attenuates atherosclerosis by regulating lipid
            metabolism through LXR-alpha/SREBP1 pathway and modulating the gut
            microbiota in ApoE-/- mice fed with HFD
  JOURNAL   J Ethnopharmacol 266, 113436 (2021)
   PUBMED   33011372
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Gertz,K., Uhlemann,R., Foryst-Ludwig,A., Barrientos,R.M.,
            Kappert,K., Thone-Reineke,C., Djoufack,P., Kirschbaum,C.,
            Fink,K.B., Heinz,A., Kintscher,U., Endres,M. and Kronenberg,G.
  TITLE     The cytoskeleton in 'couch potato-ism': Insights from a murine
            model of impaired actin dynamics
  JOURNAL   Exp Neurol 306, 34-44 (2018)
   PUBMED   29684438
REFERENCE   3  (bases 1 to 9030)
  AUTHORS   Hunkeler,M., Hagmann,A., Stuttfeld,E., Chami,M., Guri,Y.,
            Stahlberg,H. and Maier,T.
  TITLE     Structural basis for regulation of human acetyl-CoA carboxylase
  JOURNAL   Nature 558 (7710), 470-474 (2018)
   PUBMED   29899443
REFERENCE   4  (bases 1 to 9030)
  AUTHORS   Rameshreddy,P., Uddandrao,V.V.S., Brahmanaidu,P., Vadivukkarasi,S.,
            Ravindarnaik,R., Suresh,P., Swapna,K., Kalaivani,A., Parvathi,P.,
            Tamilmani,P. and Saravanan,G.
  TITLE     Obesity-alleviating potential of asiatic acid and its effects on
            ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese
            Sprague-Dawley rats
  JOURNAL   Mol Cell Biochem 442 (1-2), 143-154 (2018)
   PUBMED   28993954
  REMARK    GeneRIF: Obesity-alleviating potential of asiatic acid and its
            effects on ACC1, UCP2, and CPT1 mRNA expression in high fat
            diet-induced obese Sprague-Dawley rats.
REFERENCE   5  (bases 1 to 9030)
  AUTHORS   Li,H., Ying,H., Hu,A., Hu,Y. and Li,D.
  TITLE     Therapeutic Effect of Gypenosides on Nonalcoholic Steatohepatitis
            via Regulating Hepatic Lipogenesis and Fatty Acid Oxidation
  JOURNAL   Biol Pharm Bull 40 (5), 650-657 (2017)
   PUBMED   28458350
REFERENCE   6  (bases 1 to 9030)
  AUTHORS   Kong,I.S., Lopez-Casillas,F. and Kim,K.H.
  TITLE     Acetyl-CoA carboxylase mRNA species with or without inhibitory
            coding sequence for Ser-1200 phosphorylation
  JOURNAL   J Biol Chem 265 (23), 13695-13701 (1990)
   PUBMED   1974251
REFERENCE   7  (bases 1 to 9030)
  AUTHORS   Luo,X.C., Park,K., Lopez-Casillas,F. and Kim,K.H.
  TITLE     Structural features of the acetyl-CoA carboxylase gene: mechanisms
            for the generation of mRNAs with 5' end heterogeneity
  JOURNAL   Proc Natl Acad Sci U S A 86 (11), 4042-4046 (1989)
   PUBMED   2566999
REFERENCE   8  (bases 1 to 9030)
  AUTHORS   Lopez-Casillas,F. and Kim,K.H.
  TITLE     Heterogeneity at the 5' end of rat acetyl-coenzyme A carboxylase
            mRNA. Lipogenic conditions enhance synthesis of a unique mRNA in
            liver
  JOURNAL   J Biol Chem 264 (13), 7176-7184 (1989)
   PUBMED   2565337
REFERENCE   9  (bases 1 to 9030)
  AUTHORS   Kuts,N.L., Litovka,V.K., Andreev,A.M. and Kononuchenko,V.P.
  TITLE     [Gunshot wounds in children in peacetime]
  JOURNAL   Vestn Khir Im I I Grek 121 (11), 88-89 (1978)
   PUBMED   734871
REFERENCE   10 (bases 1 to 9030)
  AUTHORS   Bilikiewicz,A.
  TITLE     [Biological-molecular trend in psychiatry]
  JOURNAL   Pol Tyg Lek 33 (26), 1046-1050 (1978)
   PUBMED   704404
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000010.1.
            
            On Oct 25, 2022 this sequence version replaced NM_022193.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EF121986.1, J03808.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-250               JAXUCZ010000010.1  69580247-69580496
            251-383             JAXUCZ010000010.1  69595465-69595597
            384-522             JAXUCZ010000010.1  69597186-69597324
            523-632             JAXUCZ010000010.1  69603909-69604018
            633-714             JAXUCZ010000010.1  69605317-69605398
            715-813             JAXUCZ010000010.1  69607081-69607179
            814-920             JAXUCZ010000010.1  69609670-69609776
            921-1031            JAXUCZ010000010.1  69612786-69612896
            1032-1241           JAXUCZ010000010.1  69619377-69619586
            1242-1412           JAXUCZ010000010.1  69622199-69622369
            1413-1574           JAXUCZ010000010.1  69624275-69624436
            1575-1738           JAXUCZ010000010.1  69624763-69624926
            1739-1889           JAXUCZ010000010.1  69627728-69627878
            1890-1993           JAXUCZ010000010.1  69629519-69629622
            1994-2075           JAXUCZ010000010.1  69634744-69634825
            2076-2221           JAXUCZ010000010.1  69635234-69635379
            2222-2372           JAXUCZ010000010.1  69637208-69637358
            2373-2507           JAXUCZ010000010.1  69638664-69638798
            2508-2654           JAXUCZ010000010.1  69639851-69639997
            2655-2843           JAXUCZ010000010.1  69640759-69640947
            2844-2944           JAXUCZ010000010.1  69641627-69641727
            2945-3033           JAXUCZ010000010.1  69642391-69642479
            3034-3158           JAXUCZ010000010.1  69648859-69648983
            3159-3272           JAXUCZ010000010.1  69654674-69654787
            3273-3386           JAXUCZ010000010.1  69655942-69656055
            3387-3476           JAXUCZ010000010.1  69656950-69657039
            3477-3595           JAXUCZ010000010.1  69658489-69658607
            3596-3619           JAXUCZ010000010.1  69668527-69668550
            3620-3763           JAXUCZ010000010.1  69671075-69671218
            3764-3860           JAXUCZ010000010.1  69672164-69672260
            3861-3968           JAXUCZ010000010.1  69673048-69673155
            3969-4025           JAXUCZ010000010.1  69677879-69677935
            4026-4070           JAXUCZ010000010.1  69678419-69678463
            4071-4112           JAXUCZ010000010.1  69685037-69685078
            4113-4328           JAXUCZ010000010.1  69686447-69686662
            4329-4484           JAXUCZ010000010.1  69687736-69687891
            4485-4688           JAXUCZ010000010.1  69690527-69690730
            4689-4844           JAXUCZ010000010.1  69695483-69695638
            4845-4991           JAXUCZ010000010.1  69697352-69697498
            4992-5261           JAXUCZ010000010.1  69713102-69713371
            5262-5359           JAXUCZ010000010.1  69715794-69715891
            5360-5480           JAXUCZ010000010.1  69719704-69719824
            5481-5591           JAXUCZ010000010.1  69720474-69720584
            5592-5735           JAXUCZ010000010.1  69736899-69737042
            5736-5856           JAXUCZ010000010.1  69737562-69737682
            5857-5953           JAXUCZ010000010.1  69741595-69741691
            5954-6050           JAXUCZ010000010.1  69744398-69744494
            6051-6186           JAXUCZ010000010.1  69745684-69745819
            6187-6364           JAXUCZ010000010.1  69752711-69752888
            6365-6477           JAXUCZ010000010.1  69754678-69754790
            6478-6632           JAXUCZ010000010.1  69763736-69763890
            6633-6803           JAXUCZ010000010.1  69764469-69764639
            6804-6940           JAXUCZ010000010.1  69770171-69770307
            6941-9030           JAXUCZ010000010.1  69771799-69773888
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q26"
     gene            1..9030
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /note="acetyl-CoA carboxylase alpha"
                     /db_xref="GeneID:60581"
                     /db_xref="RGD:621248"
     exon            1..250
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..7064
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /EC_number="6.4.1.2"
                     /note="ACC-alpha; acetyl-coenzyme A carboxylase alpha"
                     /codon_start=1
                     /product="acetyl-CoA carboxylase 1"
                     /protein_id="NP_071529.2"
                     /db_xref="GeneID:60581"
                     /db_xref="RGD:621248"
                     /translation="MDEPSPLAKTLELNQHSRFIIGSVSEDNSEDEISNLVKLDLEEK
                     EGSLSPASVSSDTLSDLGISALQDGLAFHMRSSMSGLHLVKQGRDRKKIDSQRDFTVA
                     SPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPED
                     LKANAEYIKMADHYVPVPGGANNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLP
                     ELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKR
                     ILNVPQDLYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFR
                     QVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPA
                     AIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEM
                     VADVNLPAAQLQIAMGIPLFRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAAR
                     ITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENR
                     EEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAER
                     PDTMLGVVCGALHVADVNLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYEGIKYVL
                     KVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNK
                     TCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCI
                     HYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPQIQSTALRGEKLHRVFHYVL
                     DNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPLNVE
                     KSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQ
                     RYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSH
                     AQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASH
                     LPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRM
                     AALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFASN
                     LNHYGMTHVASVSDVLLDNAFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQS
                     PTFPESGHTSLYDEDKVPRDEPIHILNVAIKTDGDIEDDRLAAMFREFTQQNKATLVE
                     HGIRRLTFLVAQKDFRKQVNCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAF
                     QLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEA
                     SFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSM
                     VMRYGSRLWKLRVLQAELKINIRLTTTGKAIPIRLFLTNESGYYLDISLYKEVTDSRT
                     AQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIK
                     LWESMSTQAFLPSPPLPSDILTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMSLKSP
                     EYPDGRDVIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVAANSGARIGLA
                     EEIRHMFHVAWVDSEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDI
                     IGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVEN
                     SHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSY
                     MPKNVHSSVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGS
                     FSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSVPADPANLDSEAKIIQQAGQV
                     WFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECS
                     QPVMVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRKKDL
                     VKTMRRVDPVYIRLAERLGTPELSPTERKELESKLKEREEFLIPIYHQVAVQFADLHD
                     TPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHSANPELTDGQIQAMLR
                     RWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSL
                     VQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST"
     exon            251..383
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            384..522
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            523..632
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            633..714
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            715..813
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            814..920
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1031
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1241
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1412
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1574
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1575..1738
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1889
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1993
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2075
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2221
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2222..2372
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2507
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2508..2654
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2655..2843
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2844..2944
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..3033
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3034..3158
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3159..3272
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3273..3386
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3387..3476
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3477..3595
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3596..3619
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3763
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3764..3860
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3861..3968
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3969..4025
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            4026..4070
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            4071..4112
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            4113..4328
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            4329..4484
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            4485..4688
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            4689..4844
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            4845..4991
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            4992..5261
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            5262..5359
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            5360..5480
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            5481..5591
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            5592..5735
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            5736..5856
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            5857..5953
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            5954..6050
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            6051..6186
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            6187..6364
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            6365..6477
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            6478..6632
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            6633..6803
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            6804..6940
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
     exon            6941..9030
                     /gene="Acaca"
                     /gene_synonym="Acac; ACC1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctctgagagc ttatttcgaa agaataatgg atgaaccatc tccgttggcc aaaaccctgg
       61 agctgaacca gcactcccga ttcataattg ggtccgtgtc tgaagacaac tcagaagatg
      121 agatcagtaa cctggtaaag ctggacctag aggagaagga gggctccctg tcaccagcct
      181 ctgtcagctc agatacactt tctgatttgg gaatctctgc cttacaggat ggtttggcct
      241 ttcacatgag gtccagcatg tccggcttgc acctagtaaa acaaggtcga gacagaaaga
      301 aaatagactc tcaacgagat ttcactgtgg cttcgccagc agaatttgtt actcgttttg
      361 ggggaaataa agtgattgag aaggttctta tcgccaacaa tggtattgca gcagtgaaat
      421 gcatgcgatc tatccggcgg tggtcttatg aaatgttccg caatgaacgt gccatccggt
      481 ttgttgtcat ggttacaccc gaagacctta aagccaatgc agaatacatt aagatggcgg
      541 atcactatgt tccagtgcct ggaggagcaa acaacaacaa ttatgcaaat gtggaattga
      601 ttcttgatat tgcgaaaagg atacctgtac aggcagtgtg ggctggctgg ggtcatgcct
      661 ccgagaaccc caagctcccg gagctactct taaaaaatgg cattgctttc atgggccctc
      721 cgagtcaggc catgtgggcg ttgggggata agattgcatc gtctattgtg gctcaaactg
      781 caggtatccc cactcttccc tggagtggca gtggtcttcg agtggattgg caagaaaatg
      841 atttttcgaa acgcatctta aatgttccac aggatctgta tgagaaaggc tatgtgaagg
      901 atgtggatga tggactgaag gcagccgagg aggttggcta tccagtgatg atcaaggcct
      961 cagagggagg aggagggaag ggaatcagaa aagttaacaa tgcagatgac ttccctaacc
     1021 tcttcagaca ggttcaagct gaagtccctg gatcacctat atttgtaatg agattagcaa
     1081 aacagtctcg tcatctggag gtccagattc tggcggatca gtatggcaat gcaatttctt
     1141 tgtttggtcg tgactgctct gtacaacgca ggcatcagaa gatcattgaa gaagctcctg
     1201 ctgctattgc taccccagca gtatttgaac acatggaaca gtgtgctgta aaacttgcca
     1261 aaatggttgg ttatgtgagc gctgggactg tggaatactt gtacagccag gacggaagct
     1321 tctacttctt ggaactgaac cctcggctac aggttgaaca tccttgtaca gagatggtgg
     1381 ctgatgtcaa tcttcctgca gcacagctcc agattgccat ggggatccct ctatttagga
     1441 tcaaggatat tcgtatgatg tatggggtat ctccttgggg tgatgctccc attgattttg
     1501 aaaattctgc tcatgttcct tgcccaaggg gccatgtgat tgctgctcgg atcaccagtg
     1561 aaaacccaga tgaggggttt aagcccagct ctggaacagt tcaggaactt aattttcgca
     1621 gcaataagaa tgtttggggt tatttcagtg ttgctgctgc tgggggactt catgaatttg
     1681 ctgattctca gttcggtcac tgcttttcct ggggagaaaa cagagaagaa gcaatttcaa
     1741 atatggtggt ggcattgaag gagctgtcta ttcggggtga ctttcgaact acagtagaat
     1801 acctcatcaa gctgctggag acagaaagct ttcagttgaa cagaatcgac actggctggc
     1861 tggacagact gattgcagag aaagtgcagg cagagcgtcc tgacaccatg ttgggagttg
     1921 tgtgtggggc tcttcatgtg gcagatgtga acctgagaaa tagcatctct aacttccttc
     1981 actccttaga gaggggtcaa gtccttcctg ctcacacact tctgaacaca gtagatgttg
     2041 aacttatcta tgaaggaatc aaatatgtac ttaaggtgac tcggcagtct cccaactcct
     2101 acgtggtgat aatgaacggc tcgtgtgtgg aagtggacgt gcaccggctg agtgatggtg
     2161 gactgctctt gtcctacgat ggcagcagtt acaccacata catgaaggag gaggtagaca
     2221 gataccgaat cacaattggc aataaaacct gtgtgtttga gaaggaaaat gacccgtctg
     2281 taatgcgctc cccgtctgct gggaagttaa tccagtatat tgtggaagat ggaggccatg
     2341 tgtttgctgg ccagtgctat gcagagattg aggtaatgaa gatggtaatg actttgacag
     2401 ctgtagaatc tggctgcatc cattatgtca agcgacctgg agcagcactt gacccaggct
     2461 gtgtgatagc caaaatgcag ctggacaatc ccagtaaagt tcaacaggct gagcttcaca
     2521 cgggcagtct gccccagatc cagagcacag ctctccgagg cgaaaagctc catcgagttt
     2581 tccactatgt cctggataac ctggtcaatg tgatgaatgg atactgcctt ccagaccctt
     2641 tcttcagcag caaggtaaag gactgggtag aacggttaat gaagactctg agagacccct
     2701 ccctgcctct tctagaattg caggatatca tgaccagtgt ctctggccgg atccccctca
     2761 acgtggagaa gtctattaag aaggaaatgg ctcagtatgc tagcaacatc acatcggtcc
     2821 tgtgtcagtt tcccagccag cagattgcca acatcctaga tagtcatgca gctacactga
     2881 accggaaatc ggagcgggaa gtcttcttca tgaacaccca gagcattgtc cagctggtgc
     2941 agaggtaccg aagtggcatc cgtggccaca tgaaggctgt ggtgatggat ctgctccggc
     3001 agtacctgcg ggtggagaca cagtttcaga atggccacta cgacaaatgt gtattcgccc
     3061 ttcgggaaga gaacaaaagt gacatgaaca ccgtactgaa ctacatcttc tcccacgccc
     3121 aggtcaccaa gaagaatctc ctggtgacaa tgcttattga tcagttgtgt ggccgggacc
     3181 ctacacttac tgatgagctg ctaaacatcc tcacagagct aactcagctc agcaaaacca
     3241 ccaacgccaa agtggcactg cgggctcgcc aggttcttat tgcctcccat ttgccatcgt
     3301 acgagcttcg ccataaccaa gtagagtcca tcttcttatc agccatcgac atgtatggac
     3361 accagttttg cattgagaac ctgcagaaac tcatcctatc agaaacatct attttcgatg
     3421 tcctcccaaa ctttttttac cacagcaacc aggtggtgag gatggcggct ctggaggtat
     3481 atgttcgaag agcttatatc gcctatgagc tcaacagtgt acagcatcgc cagcttaagg
     3541 acaacacctg tgtggtagaa tttcagttca tgctgcccac atctcatcca aacagaggga
     3601 acatccccac gctaaacaga atgtcctttg cctccaacct caaccactac ggcatgactc
     3661 atgtagctag tgtcagcgat gttctgttgg acaacgcctt cacaccacct tgtcaacgca
     3721 tgggcgggat ggtctctttc cggacctttg aagatttcgt caggatcttt gatgaagtaa
     3781 tgggctgctt ctgcgactcc ccaccccaaa gccccacatt cccagagtcc ggtcacactt
     3841 cactctatga tgaggacaag gtccccaggg acgaaccaat acatattctg aatgtggcta
     3901 tcaagactga tggcgatatt gaggatgaca ggcttgcagc tatgttcaga gagttcaccc
     3961 aacagaataa agctactctg gttgagcatg ggatccggcg acttacgttc ctagttgcac
     4021 aaaaggattt cagaaaacaa gtcaactgtg aggtggatca gagatttcat agagaattcc
     4081 ccaaattttt cacattccga gcaagggata agtttgagga ggaccgcatt tatcgacatc
     4141 tggagcctgc tctggctttc cagttagagc tgaaccggat gagaaatttt gaccttactg
     4201 ccattccatg cgctaatcac aagatgcacc tgtaccttgg ggctgctaag gtggaagtag
     4261 gcacagaagt gactgactac aggttctttg ttcgtgcgat catcaggcac tctgatctgg
     4321 tcacgaagga agcttctttt gaatatctac aaaatgaagg agagcgactg ctcctggaag
     4381 ccatggatga attggaagtt gctttcaata acacaaatgt tcgcacagac tgtaaccata
     4441 tattcctcaa ctttgtgccc acagtcatca tggacccatc aaagattgaa gaatctgtgc
     4501 ggagcatggt aatgcgctat ggaagccggc tgtggaaatt gcgggtcctc caggcagaac
     4561 tgaaaatcaa cattcgcctg acaacaactg gaaaagcgat tcccatccgc ctcttcctga
     4621 caaacgagtc tggctactac ttggacatca gcctgtataa ggaagtgact gactccagga
     4681 cagcacagat catgtttcag gcgtatggag acaagcaggg accactgcat ggaatgttaa
     4741 tcaatactcc gtatgtgacc aaagaccttc ttcaatcaaa gaggtttcag gcacagtcct
     4801 tgggaacaac gtatatatat gatatcccag agatgtttcg gcagtcgctc atcaagctct
     4861 gggagtccat gtccactcaa gcatttcttc cttcaccccc tttgccttcc gacatactga
     4921 cgtatactga actggtgttg gatgatcaag gccagcttgt ccatatgaac agacttccag
     4981 gaggaaacga gattggcatg gtagcctgga aaatgagcct taaaagccct gaatatccag
     5041 atggccgaga tgtcattgtc atcggcaatg acattacata tcggattggc tcctttgggc
     5101 ctcaggaaga tttgctgttt ctcagagctt ctgaacttgc cagagcagaa ggcatcccac
     5161 gcatctacgt agcagccaac agtggagcta gaattggact ggcagaagaa atccgtcata
     5221 tgttccacgt ggcctgggta gactctgagg atccttacaa gggatacaag tatttatatc
     5281 tgacacccca ggattataaa agagtgagtg ctctcaattc tgtccactgt gaacatgtgg
     5341 aagatgaagg agaatccagg tacaagataa cagacattat cgggaaagaa gaaggacttg
     5401 gagcagagaa ccttcggggt tctggaatga ttgctgggga atcctcattg gcttatgatg
     5461 agatcatcac catcagcctg gttacatgcc gggccattgg tattggggct taccttgtcc
     5521 ggctgggaca aagaaccatc caggttgaga actctcactt aattctaaca ggagccggtg
     5581 ccctcaacaa agtccttggt cgggaagtat acacctccaa caatcagctt gggggcatcc
     5641 agataatgca caacaacgga gttacccatt gcactgtttg tgatgacttt gagggagtgt
     5701 tcacagtctt acactggctg tcatacatgc ctaagaacgt gcacagttca gttcctctcc
     5761 tgaattccaa ggatcctata gatagaatca tcgagtttgt tcccacaaag gccccgtatg
     5821 atcctcggtg gatgctggca ggccgtcctc acccaaccca gaaaggccaa tggttgagtg
     5881 gattttttga ttatggctct ttctcagaaa tcatgcagcc ctgggcgcag accgtggtag
     5941 ttggcagagc caggttgggg ggaatacctg tgggagtagt tgctgtagaa acccgaaccg
     6001 tggagctcag tgtaccagct gatcctgcaa acctggattc tgaagccaag ataatccagc
     6061 aggccggcca agtttggttt ccagactctg catttaagac ctatcaagct atcaaggact
     6121 ttaaccgtga agggctacct ctaatggtct ttgccaactg gagaggcttc tctggtggga
     6181 tgaaagatat gtatgaccag gtgctcaagt ttggtgctta tattgtggat ggcttgcggg
     6241 aatgttccca gcctgtgatg gtctacattc ccccacaggc tgagcttcgg ggtggttctt
     6301 gggttgtgat cgacccaacc atcaatcctc ggcacatgga gatgtatgct gaccgggaaa
     6361 gcaggggatc cgttctggaa ccagaaggga cagtagaaat caaattccgc aaaaaggatc
     6421 tggtgaaaac catgcgtcgc gtagacccag tctacatccg cttggctgag cgactgggca
     6481 ccccagagct aagccccact gagcggaagg agctggagag caagttgaag gagcgggagg
     6541 agttcctaat tcccatttac catcaggtag ctgtgcagtt tgctgacttg cacgacaccc
     6601 caggccggat gcaggagaag ggtgtcatta atgatatctt agattggaaa acatcccgca
     6661 ccttcttcta ctggcgactg aggcgtctcc tgctggaaga cctggtcaag aagaaaatcc
     6721 acagtgccaa ccctgagctg accgatggcc agatccaggc catgttgaga cgctggtttg
     6781 tggaagtgga aggcacagtg aaggcttacg tctgggacaa taataaggat ctggtggaat
     6841 ggctggagaa gcagctgaca gaggaagatg gtgtccgctc tgtgatagag gagaacatca
     6901 aatacatcag cagggactat gtcctcaagc agatccgcag cttggtgcag gccaatccag
     6961 aagttgccat ggactccatc gtccacatga cccagcacat ctcccccact cagcgagcag
     7021 aggttgtaag gatcctttcc actatggact ccccttctac gtaggaggaa cttcccaccc
     7081 atccccgccc tgtctccgga gaagagagct ggggctgcct ctcccatctg aaaccactgt
     7141 aaggagaagg caccggaggc ctgggactgg agtccgtggc atttgcttcc ccttgagtgt
     7201 ttcagggtct gcatgacatc ctgggctata ggatcacaaa gcccacccac gcatacccag
     7261 ttcagtattt attagcccag ctgtgatgac agtcctcttt ccatgcccag gactgagaag
     7321 gcaatgaaag gtacacgcct gtaccatgag gtcttactac tgcaggcctg ccctcctgtc
     7381 ctgcctgccc cagcataggg tcgtgtgaac agtgtccaag tgtctgcagc tccctgcccc
     7441 atgagccaca gccataggca gcaggggagg gctggggctg aggaaggagg aaactcaagt
     7501 ccatcactca ctaccccaca cacagccaag cacataccag gcactggaga ggaacggtcc
     7561 ccgtctaacc acagcaagat aactatttac gagaaatgtc agagcagaaa acctgtcaca
     7621 ggcccacaag aacacagaga tgagggcaaa gccatgatct aacaggtaag ctcaaaacca
     7681 gaccagacca tgtcattgtt cctggtcaac actttcaacc acctcgaatg gtgtcgcagt
     7741 acccctgggg caatgatcaa cctgccacca ccttccctat cgctgtgtag tcacatagtg
     7801 actataccta ggagctaaag gccctggcct ctgtgggtag atggggacag gcaggcagtg
     7861 caggagcctg aggtggggag ctgagaagag gcgctttcct gttggtgata gggagagagt
     7921 agctcaagac aggagaagtc tcttctgggg acagttgtca ctgtggggcc atgcctattc
     7981 ctggcagaag gtgtggcagg ctacccacct gtgacacctg tgctttcagc accagaactg
     8041 gccttcctga tgttaggagg cttcccggga acacgggttc acgtgccctt aagatacctt
     8101 acaggtcagc ctcagtgctg gtgtttccag actctctggg ccctgcatgg ccagcagcag
     8161 ggcctcctca agggtggcct gaagagttta acaacccatt tacccaggag agcagcttac
     8221 gggtcagcat tggtgttcct aaagcaagga tggggatgat cagagctggt gtaacatcca
     8281 gtttctactt tgactgtcgc tgaagatcaa ggaggaaaaa aatgcccgcc acagaaacca
     8341 ttccagtgag cgcttcagtc tgagccttaa cctggactcc acaaaaagag ctgacctccc
     8401 attcaggagc acctcgagca gatattccag aggaccttct caatggtgcc acattgcaag
     8461 cggacatcca agggactata gatgcaagtt gcccaactcc tacaagcgaa caggagggcc
     8521 agggccagat caggtgggga gcatcctcag ttagaaggaa gctccctggc ccctggttct
     8581 cagctcacct tgtaggtaag ggtgggattt gtctccagcc tctacacttt gctacggcac
     8641 atccagtccc acacccagtg gtatgaaggc tgctttcctg ccgttccttc tgaggtgaag
     8701 tgggttgtgc ttgtataact cccttttccc tgctgcccca tccccaggac cggggctcct
     8761 ccatggagaa gtcagagcca cagcacatga gcagtctgat ggccccacag cccattacac
     8821 atgcaaggtt actgagggag gaggatgaat gatgtgttga cccagacttg cttgaactga
     8881 gattgccata ttatctgtcc acttgttcac aaagcagcct tcactcgtgt cagtgagaat
     8941 tcgatggaca cacacgccaa cgagcagggg cttcagtcca atgcactctt ttcacgtttt
     9001 gttgaaataa acctccacat ttgtagaaga
//
