LOCUS       NM_133411               4526 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus ATP binding cassette subfamily C member 4
            (Abcc4), mRNA.
ACCESSION   NM_133411 XM_341375
VERSION     NM_133411.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Furtado,A., Mineiro,R., Duarte,A.C., Goncalves,I., Santos,C.R. and
            Quintela,T.
  TITLE     The Daily Expression of ABCC4 at the BCSFB Affects the Transport of
            Its Substrate Methotrexate
  JOURNAL   Int J Mol Sci 23 (5), 2443 (2022)
   PUBMED   35269592
  REMARK    GeneRIF: The Daily Expression of ABCC4 at the BCSFB Affects the
            Transport of Its Substrate Methotrexate.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Akanuma,S.I., Hashimoto,K., Yoshida,Y., Kubo,Y. and Hosoya,K.I.
  TITLE     Inflammation-Induced Attenuation of Prostaglandin D2 Elimination
            across Rat Blood-Brain Barrier: Involvement of the Downregulation
            of Organic Anion Transporter 3 and Multidrug Resistance-Associated
            Protein 4
  JOURNAL   Biol Pharm Bull 43 (11), 1669-1677 (2020)
   PUBMED   33132311
  REMARK    GeneRIF: Inflammation-Induced Attenuation of Prostaglandin D2
            Elimination across Rat Blood-Brain Barrier: Involvement of the
            Downregulation of Organic Anion Transporter 3 and Multidrug
            Resistance-Associated Protein 4.
REFERENCE   3  (bases 1 to 4526)
  AUTHORS   Xia,W., Zhang,H., Pan,Z., Li,G., Zhou,Q., Hu,D. and Liu,Y.
  TITLE     Inhibition of MRP4 alleviates sepsis-induced acute lung injury in
            rats
  JOURNAL   Int Immunopharmacol 72, 211-217 (2019)
   PUBMED   30995593
  REMARK    GeneRIF: These data suggest that inhibition of MRP4 significantly
            alleviates sepsis-induced acute lung injury in rats.
            Erratum:[Int Immunopharmacol. 2023 Sep;122:110699. doi:
            10.1016/j.intimp.2023.110699.. PMID: 37500384]
REFERENCE   4  (bases 1 to 4526)
  AUTHORS   Zhu,L., Wang,L., Cao,F., Liu,P., Bao,H., Yan,Y., Dong,X., Wang,D.,
            Wang,Z. and Gong,P.
  TITLE     Modulation of transport and metabolism of bile acids and bilirubin
            by chlorogenic acid against hepatotoxicity and cholestasis in bile
            duct ligation rats: involvement of SIRT1-mediated deacetylation of
            FXR and PGC-1alpha
  JOURNAL   J Hepatobiliary Pancreat Sci 25 (3), 195-205 (2018)
   PUBMED   29360226
REFERENCE   5  (bases 1 to 4526)
  AUTHORS   Han,H., Xu,L., Xiong,K., Zhang,T. and Wang,Z.
  TITLE     Exploration of Hepatoprotective Effect of Gentiopicroside on
            Alpha-Naphthylisothiocyanate-Induced Cholestatic Liver Injury in
            Rats by Comprehensive Proteomic and Metabolomic Signatures
  JOURNAL   Cell Physiol Biochem 49 (4), 1304-1319 (2018)
   PUBMED   30223280
REFERENCE   6  (bases 1 to 4526)
  AUTHORS   Chen,C. and Klaassen,C.D.
  TITLE     Rat multidrug resistance protein 4 (Mrp4, Abcc4): molecular
            cloning, organ distribution, postnatal renal expression, and
            chemical inducibility
  JOURNAL   Biochem Biophys Res Commun 317 (1), 46-53 (2004)
   PUBMED   15047146
REFERENCE   7  (bases 1 to 4526)
  AUTHORS   Denk,G.U., Soroka,C.J., Takeyama,Y., Chen,W.S., Schuetz,J.D. and
            Boyer,J.L.
  TITLE     Multidrug resistance-associated protein 4 is up-regulated in liver
            but down-regulated in kidney in obstructive cholestasis in the rat
  JOURNAL   J Hepatol 40 (4), 585-591 (2004)
   PUBMED   15030973
  REMARK    GeneRIF: Obstructive cholestasis results in progressive
            up-regulation of Mrp4 protein in liver but down-regulation in
            kidney. The absence of corresponding changes in Mrp4 mRNA suggests
            posttranscriptional mechanisms as predominant regulators of Mrp4
            expression.
REFERENCE   8  (bases 1 to 4526)
  AUTHORS   Rius,M., Nies,A.T., Hummel-Eisenbeiss,J., Jedlitschky,G. and
            Keppler,D.
  TITLE     Cotransport of reduced glutathione with bile salts by MRP4 (ABCC4)
            localized to the basolateral hepatocyte membrane
  JOURNAL   Hepatology 38 (2), 374-384 (2003)
   PUBMED   12883481
  REMARK    GeneRIF: MRP4 can mediate the efflux of reduced glutatione from
            hepatocytes into blood by cotransport with monoanionic bile salts.
REFERENCE   9  (bases 1 to 4526)
  AUTHORS   Hagenbuch,B., Gao,B. and Meier,P.J.
  TITLE     Transport of xenobiotics across the blood-brain barrier
  JOURNAL   News Physiol Sci 17, 231-234 (2002)
   PUBMED   12433976
  REMARK    Review article
REFERENCE   10 (bases 1 to 4526)
  AUTHORS   van Aubel,R.A.M.H., Smeets,P.H.E., Peters,J.G.P., Bindels,R.J.M.
            and Russel,F.G.M.
  TITLE     The MRP4/ABCC4 gene encodes a novel apical organic anion
            transporter in human kidney proximal tubules: putative efflux pump
            for urinary cAMP and cGMP
  JOURNAL   J Am Soc Nephrol 13 (3), 595-603 (2002)
   PUBMED   11856762
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY533524.1.
            
            On Mar 26, 2005 this sequence version replaced XM_341375.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY533524.1, AF534126.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15q24"
     gene            1..4526
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /note="ATP binding cassette subfamily C member 4"
                     /db_xref="GeneID:170924"
                     /db_xref="RGD:620266"
     CDS             1..3978
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /EC_number="7.6.2.2"
                     /EC_number="7.6.2.3"
                     /note="multidrug resistance-associated protein 4;
                     ATP-binding cassette, subfamily C (CFTR/MRP), member 4;
                     ABC-tranporter; ATP-binding cassette protein C4;
                     ATP-binding cassette, sub-family C (CFTR/MRP), member 4"
                     /codon_start=1
                     /product="ATP-binding cassette subfamily C member 4"
                     /protein_id="NP_596902.1"
                     /db_xref="GeneID:170924"
                     /db_xref="RGD:620266"
                     /translation="MLPVHTEVKPNPLQDANLCSRLFFWWLNPLFKAGHKRRLEEDDM
                     FSVLPEDRSKHLGEELQGYWDKEVLRAKKDARKPSLTKAIVKCYWKSYLILGIFTLIE
                     ETTRVVQPIFLGKIIDYFEKYDSDDSAALHTAYGYAAVLSLCTLILAILHHLYFYHVQ
                     CAGMRIRVAMCHMIYRKALRLSNSAMGKTTTGQIVNLLSNDVNKFDQVTIFLHFLWAG
                     PLQAIGVTILLWVEIGISCLAGLAILVILLPLQSCIGKLFSSLRSKTAAFTDARFRTM
                     NEVITGMRIIKMYAWEKSFADLITNLRKKEISKILGSSYLRGMNMASFFIANKVILFV
                     TFTTYVLLGNKITSSHVFVAMTLYGAVRLTVTLFFPSAIERVSEAVVSVRRIKNFLLL
                     DELPERKAQEPSDGKAIVHVQDFTAFWDKALDTPTLQGLSFTARPGELLAVVGPVGAG
                     KSSLLSAVLGELPPTSGLVSVHGRIAYVSQQPWVFSGTVRSNILFGRKYEKERYEKVI
                     KACALKKDLQLLEDGDLTVIGDRGATLSGGQKARVNLARAVYQDADIYLLDDPLSAVD
                     AEVGKHLFQLCICQTLHEKITILVTHQLQYLKAASHILILKDGEMVQKGTYTEFLKSG
                     VDFGSLLKKENEEAEPSPVPGTPTLRNRTFSEASIWSQQSSRPSLKDGVPDAQDAENT
                     QAAQPEESRSEGRIGFKAYKNYFSAGASWFFIIFLVLLNLMGQVFYVLQDWWLSHWAN
                     RQGALNDTKNANGNVTGTLDLSWYLGIYTGLTAVTVLFGIARSLLVFYVLVNASQTLH
                     NRMFESILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTFLDFIQTLLLVVSVIAV
                     AAAVIPWILIPLVPLSIIFVVLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQGLWTI
                     RAYKAEERCQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAVFVIVVAFGSLVLAK
                     TLDAGQVGLALSYSLTLMGMFQWSVRQSAEVENMMISVERVIEYTDLEKEAPWECRKR
                     PPPGWPHEGVIVFDNVNFTYSLDGPLVLKHLTALIKSREKVGIVGRTGAGKSSLISAL
                     FRLSEPEGKIWIDKILTTEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHSDEEL
                     WKALEEVQLKEAIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILKKNRILIIDEAT
                     ANVDPRTDELIQQKIREKFAQCTVLTIAHRLNTIIDSDKIMVLDSGRLREYDEPYVLL
                     QNPESLFYKMVQQLGKGEAAALTETAKQVYFRRNYPDIAFSSPAVMSTSNGQPSALTI
                     FETAL"
     exon            1..74
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            75..185
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            186..306
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            307..531
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            532..621
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            622..785
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            786..911
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            912..1161
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1263
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1353
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1545
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1640
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1727
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1824
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..2034
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2175
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2176..2213
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2308
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..2455
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2535
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2536..2686
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2687..2806
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2807..2917
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..3018
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            3019..3210
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            3211..3366
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            3367..3456
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            3457..3629
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3735
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            3736..3870
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
     exon            3871..4512
                     /gene="Abcc4"
                     /gene_synonym="Mrp4; RGD1565953"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgctgccgg tgcacaccga ggtgaaaccc aacccgctgc aggacgccaa cctctgctcg
       61 cgcttgttct tctggtggct caacccgttg tttaaagctg gacataagcg gagattggaa
      121 gaagatgaca tgttttcagt gcttccagaa gatcgctcaa agcaccttgg agaggagttg
      181 caagggtact gggataaaga agttctgcga gccaagaagg acgctcggaa gccttcctta
      241 acgaaggcaa tcgtgaagtg ttactggaaa tcttacctga ttttgggaat ttttacgtta
      301 attgaggaga ccacccgagt agttcagccc atatttttag ggaaaattat tgattatttt
      361 gagaagtatg actctgacga ctcggccgct ttgcacacag cttacggcta cgcggcggtg
      421 ctgtcgctgt gcacgctcat cctggccata ctacatcact tgtatttcta ccacgtgcag
      481 tgcgccggga tgaggatacg ggtcgccatg tgccacatga tttaccggaa ggctctccgg
      541 ctaagtaact cggccatggg gaagacaacc acgggccaga tagtcaacct gctgtccaac
      601 gacgtgaaca agtttgacca ggtgaccatc ttcttgcact tcctgtgggc ggggccgctg
      661 caagccatag gggtaaccat ccttctctgg gtggaaatcg gaatctcctg tctggcgggc
      721 ctggccattc tggttattct gctgcctctg caaagctgca tcgggaaact gttctcgtca
      781 ctgcggagta aaacagcggc tttcacggat gccaggttcc ggaccatgaa tgaagtgata
      841 acgggcatga ggataataaa gatgtacgcg tgggagaagt cattcgccga cctcataacc
      901 aatctgagaa agaaggaaat ttctaagatt ctgggcagct cctacctcag agggatgaac
      961 atggcgtcgt ttttcattgc aaacaaagtc atcctgttcg tgaccttcac cacctatgtg
     1021 ctgcttggca ataagattac atctagccac gtgttcgtgg ccatgactct gtatggtgct
     1081 gtccggttga cagttaccct cttcttccca tcagccattg agagagtgtc agaggccgtc
     1141 gtcagcgttc ggaggatcaa gaactttctg ttactcgatg aactgccaga gcgcaaagcc
     1201 caggaaccat ctgatggcaa agccatcgtt cacgtgcaag attttaccgc tttctgggac
     1261 aaggcattag acactcctac cctgcaaggt ctttccttca ctgccagacc tggtgagttg
     1321 ttagctgtgg tcggcccagt cggagcaggc aagtcgtccc tgttgagcgc ggtgctcggt
     1381 gagctgcctc ccaccagtgg gctggtcagc gtgcacggga ggatcgccta cgtttcccag
     1441 cagccctggg tgttctcagg aaccgtgagg agcaatattt tatttgggag gaaatacgag
     1501 aaagaacgct acgagaaagt catcaaggcc tgtgctctga agaaggactt gcaacttctg
     1561 gaggacgggg atctgactgt cataggcgac cggggagcca cgctgagcgg aggccagaaa
     1621 gcacgggtga acttggcacg ggcagtctac caggatgccg acatctacct cctcgatgat
     1681 ccgctcagtg ctgttgatgc agaagtgggc aagcacctgt tccagctgtg tatctgccag
     1741 actttgcacg agaagatcac cattttagtg actcaccagt tacagtacct caaagctgca
     1801 agccacatcc tcatactaaa agatggcgaa atggtgcaga aggggactta caccgagttt
     1861 ctgaagtctg gtgtagattt cggttccctg ctaaagaagg aaaatgagga agcagagccc
     1921 tcccctgtcc caggaacacc gacactcagg aaccgaacct tctccgaggc ctcgatttgg
     1981 tctcaacagt catccagacc ctcgttgaaa gacggggtcc cagatgccca agatgcagag
     2041 aatactcagg cagctcaacc cgaggagagc cgttcagaag ggagaatcgg cttcaaggcc
     2101 tacaagaact acttctctgc aggcgcatcc tggtttttca ttattttcct cgttctgctt
     2161 aacctgatgg gtcaggtttt ctacgttctt caggactggt ggctttccca ctgggcgaat
     2221 aggcaaggtg ccctgaatga caccaaaaac gcgaacggga atgtaaccgg gaccctcgat
     2281 ctcagctggt acttagggat ttacacaggt ttaactgcgg tcacagtcct ctttggcata
     2341 gcgagatccc tgctggtgtt ctatgtcctt gtgaatgctt cccagactct gcacaacagg
     2401 atgttcgagt cgatcctgaa ggcaccggtg ttgttcttcg atagaaatcc aataggaagg
     2461 attttaaatc gtttctccaa ggacatcgga cacatggacg acttgcttcc cctaacgttt
     2521 ctagacttca tccagacctt gctccttgtt gtaagtgtga tcgccgtggc ggcggccgtg
     2581 atcccttgga tcctcatacc cctggttcca ctctccatca tctttgtggt ccttcggaga
     2641 tatttcttag aaacgtcgcg ggacgtcaag cgcctggagt ccacaacacg gagcccggta
     2701 ttctcccatt tatcatcctc cctccaggga ctctggacca tccgtgccta taaggctgaa
     2761 gagaggtgtc aggagctgtt tgatgcacac caggacttgc attcagaggc ttggttcttg
     2821 ttcctgacga catcaagatg gttcgccgtg cgtctggacg ccatctgcgc cgtctttgtc
     2881 atcgtcgttg ccttcgggtc cctggttctg gcaaagacct tggatgctgg gcaggttggc
     2941 ttggccttgt cctactccct cacgctcatg gggatgttcc agtggtctgt gagacagagc
     3001 gccgaagtag agaatatgat gatttctgtg gagagggtga ttgaatacac ggacctagag
     3061 aaggaggcgc cttgggaatg caggaagcgc ccacccccag gctggcccca cgaaggagta
     3121 attgtcttcg acaatgtgaa cttcacctac agcttggacg ggccccttgt tctgaagcac
     3181 ctgaccgcac tcatcaagtc acgagaaaag gttggaattg tgggaagaac tggagctgga
     3241 aaaagttccc tcatctcagc cctcttcagg ttgtcagaac ctgaggggaa aatctggatt
     3301 gataagatct tgacgactga aattggactt cacgatttaa ggaagaaaat gtcaatcata
     3361 cctcaggaac ctgtcctgtt cactggaacc atgaggaaga acctggaccc ctttaatgag
     3421 cactcggacg aggagctgtg gaaagcccta gaggaggtac aacttaaaga ggccatagaa
     3481 gatcttcctg gtaaaatgga cactgaacta gcagaatctg gatccaattt cagtgttgga
     3541 cagagacagt tagtgtgcct tgcgagggca attctgaaga aaaaccggat actgatcatt
     3601 gatgaagcaa ctgcaaatgt ggatccgaga actgacgagt taatacaaca gaaaatccgg
     3661 gagaagtttg cccagtgcac tgttctcacc attgctcaca gactgaacac catcattgac
     3721 agcgacaaga taatggtttt ggattcggga agactgagag aatatgatga gccgtatgtt
     3781 ttgctgcaga atccagagag cctcttttac aagatggttc agcagctggg taagggcgaa
     3841 gccgctgccc tcaccgaaac agcaaaacag gtgtacttca gacggaatta tccggatatt
     3901 gcattcagca gccctgcggt tatgagcacc tccaacggac agccctccgc cttaacgata
     3961 tttgaaacag cattgtgact actaccagga cgtcaagtct gtttccaggg tgtcccctgc
     4021 cccaacaagg ggctcgcact tcttagacta cagcactcct ggtcttcatt ctgcaacaga
     4081 tccttcacag acaagagacc ggcgtctttg gactttcccc ttcgacctta tcctgtgact
     4141 tcaggtccta acaacatggc ctgttgtttt atttaaatgt tcctactgtt ctttacaaat
     4201 ccagttctga ggtgcaggcc actgacaaca gctgcctacc aggttttgtg ccttaacaga
     4261 ctctagggcc aaagcccgtg cgttttataa gctgtcacag tgggttgttt cttcctcttg
     4321 ccttaaaagt acactctccg ggtgcgtcag ggactctgtt tacttgaaga cggtgtgcag
     4381 atgccagttt gtttccctgc tttctctgac ttagctagga cccgttactt ctcccacaaa
     4441 ggcctctggt taaaacagaa cagataaaat taacaggaac cacaacaaca aaccaaaaaa
     4501 aaaaaaaaaa aaaaaaaaaa aaaaaa
//
