LOCUS       NM_012833               5073 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Rattus norvegicus ATP binding cassette subfamily C member 2
            (Abcc2), mRNA.
ACCESSION   NM_012833 XM_346593
VERSION     NM_012833.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Zecchinati,F., Barranco,M.M., Tocchetti,G.N., Dominguez,C.J.,
            Arana,M.R., Perdomo,V.G., Mottino,A.D., Garcia,F. and
            Villanueva,S.S.M.
  TITLE     Multidrug resistance-associated protein 2 is negatively regulated
            by oxidative stress in rat intestine via a posttranslational
            mechanism. Impact on its membrane barrier function
  JOURNAL   Toxicology 460, 152873 (2021)
   PUBMED   34303734
  REMARK    GeneRIF: Multidrug resistance-associated protein 2 is negatively
            regulated by oxidative stress in rat intestine via a
            posttranslational mechanism. Impact on its membrane barrier
            function.
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Tocchetti,G.N., Dominguez,C.J., Zecchinati,F., Arana,M.R.,
            Rigalli,J.P., Ruiz,M.L., Villanueva,S.S.M. and Mottino,A.D.
  TITLE     Intraluminal nutrients acutely strengthen rat intestinal MRP2
            barrier function by a glucagon-like peptide-2-mediated mechanism
  JOURNAL   Acta Physiol (Oxf) 230 (4), e13514 (2020)
   PUBMED   32476256
  REMARK    GeneRIF: Intraluminal nutrients acutely strengthen rat intestinal
            MRP2 barrier function by a glucagon-like peptide-2-mediated
            mechanism.
REFERENCE   3  (bases 1 to 5073)
  AUTHORS   Tocchetti,G.N., Arias,A., Arana,M.R., Rigalli,J.P., Dominguez,C.J.,
            Zecchinati,F., Ruiz,M.L., Villanueva,S.S.M. and Mottino,A.D.
  TITLE     Acute regulation of multidrug resistance-associated protein 2
            localization and activity by cAMP and
            estradiol-17beta-D-glucuronide in rat intestine and Caco-2 cells
  JOURNAL   Arch Toxicol 92 (2), 777-788 (2018)
   PUBMED   29052767
  REMARK    GeneRIF: we demonstrate redistribution of intestinal MRP2 between
            plasma membrane and intracellular membrane in rat jejunum and in
            Caco-2 cells in response to db-cAMP and
            estradiol-17beta-D-glucuronide
REFERENCE   4  (bases 1 to 5073)
  AUTHORS   Qu,X., Zhang,Y., Zhang,S., Zhai,J., Gao,H., Tao,L. and Song,Y.
  TITLE     Dysregulation of BSEP and MRP2 May Play an Important Role in
            Isoniazid-Induced Liver Injury via the SIRT1/FXR Pathway in Rats
            and HepG2 Cells
  JOURNAL   Biol Pharm Bull 41 (8), 1211-1218 (2018)
   PUBMED   30068870
  REMARK    GeneRIF: In conclusion, our data supports the hypothesis that
            isoniazid can induce liver injury via the down-regulation of
            hepatic efflux transporters BSEP and MRP2, and the SIRT1/FXR
            pathway may be the primary regulator with regard to the expression
            of transporters and the biosynthesis of bile acids in the liver.
REFERENCE   5  (bases 1 to 5073)
  AUTHORS   Arana,M.R., Tocchetti,G.N., Zecchinati,F., Londero,A.S.,
            Dominguez,C., Perdomo,V., Rigalli,J.P., Villanueva,S.S.M. and
            Mottino,A.D.
  TITLE     Glucagon-like peptide 2 prevents down-regulation of intestinal
            multidrug resistance-associated protein 2 and P-glycoprotein in
            endotoxemic rats
  JOURNAL   Toxicology 390, 22-31 (2017)
   PUBMED   28842383
  REMARK    GeneRIF: GLP-2 can prevent down-regulation of intestinal expression
            and activity of Mrp2 and P-gp in endotoxemic rats.
REFERENCE   6  (bases 1 to 5073)
  AUTHORS   Zhang,Y., Li,W. and Vore,M.
  TITLE     Translational regulation of rat multidrug resistance-associated
            protein 2 expression is mediated by upstream open reading frames in
            the 5' untranslated region
  JOURNAL   Mol Pharmacol 71 (1), 377-383 (2007)
   PUBMED   17065236
REFERENCE   7  (bases 1 to 5073)
  AUTHORS   Buchler,M., Konig,J., Brom,M., Kartenbeck,J., Spring,H., Horie,T.
            and Keppler,D.
  TITLE     cDNA cloning of the hepatocyte canalicular isoform of the multidrug
            resistance protein, cMrp, reveals a novel conjugate export pump
            deficient in hyperbilirubinemic mutant rats
  JOURNAL   J Biol Chem 271 (25), 15091-15098 (1996)
   PUBMED   8662992
REFERENCE   8  (bases 1 to 5073)
  AUTHORS   Paulusma,C.C., Bosma,P.J., Zaman,G.J., Bakker,C.T., Otter,M.,
            Scheffer,G.L., Scheper,R.J., Borst,P. and Oude Elferink,R.P.
  TITLE     Congenital jaundice in rats with a mutation in a multidrug
            resistance-associated protein gene
  JOURNAL   Science 271 (5252), 1126-1128 (1996)
   PUBMED   8599091
REFERENCE   9  (bases 1 to 5073)
  AUTHORS   Mayer,R., Kartenbeck,J., Buchler,M., Jedlitschky,G., Leier,I. and
            Keppler,D.
  TITLE     Expression of the MRP gene-encoded conjugate export pump in liver
            and its selective absence from the canalicular membrane in
            transport-deficient mutant hepatocytes
  JOURNAL   J Cell Biol 131 (1), 137-150 (1995)
   PUBMED   7559771
REFERENCE   10 (bases 1 to 5073)
  AUTHORS   Kojima,H., Omoto,Y., Hidaka,H., Aoki,T., Nakamura,T., Kawabata,T.,
            Konaka,K., Kashiwagi,A., Kikkawa,R. and Shigeta,Y.
  TITLE     Increased plasma post-heparin diamine oxidase activity and plant
            sterol levels in streptozotocin diabetic rat
  JOURNAL   Biochem Biophys Res Commun 186 (1), 398-404 (1992)
   PUBMED   1632778
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000001.1, L49379.1 and
            X96393.1.
            
            On Aug 27, 2014 this sequence version replaced NM_012833.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X96393.1, D86086.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760400, SAMEA5760476
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            regulatory uORF        :: PMID: 17065236
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-159               JAXUCZ010000001.1  252613875-252614033
            160-5037            L49379.1           41-4918
            5038-5073           X96393.1           4874-4909
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q54"
     gene            1..5073
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /note="ATP binding cassette subfamily C member 2"
                     /db_xref="GeneID:25303"
                     /db_xref="RGD:2366"
     exon            1..243
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      105..275
                     /regulatory_class="uORF"
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:17065236]"
                     /note="this uORF is predicted to encode a 56 aa peptide
                     (PMID:17065236)"
     CDS             214..4839
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /EC_number="7.6.2.2"
                     /EC_number="7.6.2.3"
                     /note="canalicular multispecific organic anion transporter
                     1; canalicular multidrug resistance protein; cMrp2/cMoat
                     canalicular apical conjugate export pump; multidrug
                     resistance-associated protein 2; ATP-binding cassette,
                     sub-family C (CFTR/MRP), member 2; calicular multispecific
                     organic anion transporter; ATP-binding cassette, subfamily
                     C (CFTR/MRP), member 2"
                     /codon_start=1
                     /product="ATP-binding cassette sub-family C member 2"
                     /protein_id="NP_036965.1"
                     /db_xref="GeneID:25303"
                     /db_xref="RGD:2366"
                     /translation="MDKFCNSTFWDLSLLESPEADLPLCFEQTVLVWIPLGFLWLLAP
                     WQLYSVYRSRTKRSSITKFYLAKQVFVVFLLILAAIDLSLALTEDTGQATVPPVRYTN
                     PILYLCTWLLVLAVQHSRQWCVRKNSWFLSLFWILSVLCGVFQFQTLIRALLKDSKSN
                     MAYSYLFFVSYGFQIVLLILTAFSGPSDSTQTPSVTASFLSSITFSWYDRTVLKGYKH
                     PLTLEDVWDIDEGFKTRSVTSKFEAAMTKDLQKARQAFQRRLQKSQRKPEATLHGLNK
                     KQSQSQDVLVLEEAKKKSEKTTKDYPKSWLIKSLFKTFHVVILKSFILKLIHDLLVFL
                     NPQLLKLLIGFVKSSNSYVWFGYICAILMFAVTLIQSFCLQSYFQHCFVLGMCVRTTV
                     MSSIYKKALTLSNLARKQYTIGETVNLMSVDSQKLMDATNYMQLVWSSVIQITLSIFF
                     LWRELGPSILAGVGVMVLLIPVNGVLATKIRNIQVQNMKNKDKRLKIMNEILSGIKIL
                     KYFAWEPSFQEQVQGIRKKELKNLLRFGQLQSLLIFILQITPILVSVVTFSVYVLVDS
                     ANVLNAEKAFTSITLFNILRFPLSMLPMVTSSILQASVSVDRLERYLGGDDLDTSAIR
                     RVSNFDKAVKFSEASFTWDPDLEATIQDVNLDIKPGQLVAVVGTVGSGKSSLVSAMLG
                     EMENVHGHITIQGSTAYVPQQSWIQNGTIKDNILFGSEYNEKKYQQVLKACALLPDLE
                     ILPGGDMAEIGEKGINLSGGQKQRVSLARAAYQDADIYILDDPLSAVDAHVGKHIFNK
                     VVGPNGLLAGKTRIFVTHGIHFLPQVDEIVVLGKGTILEKGSYRDLLDKKGVFARNWK
                     TFMKHSGPEGEATVNNDSEAEDDDDGLIPTMEEIPEDAASLAMRRENSLRRTLSRSSR
                     SSSRRGKSLKNSLKIKNVNVLKEKEKEVEGQKLIKKEFVETGKVKFSIYLKYLQAVGW
                     WSILFIILFYGLNNVAFIGSNLWLSAWTSDSDNLNGTNNSSSHRDMRIGVFGALGLAQ
                     GICLLISTLWSIYACRNASKALHGQLLTNILRAPMRFFDTTPTGRIVNRFSGDISTVD
                     DLLPQTLRSWMMCFFGIAGTLVMICMATPVFAIIIIPLSILYISVQVFYVATSRQLRR
                     LDSVTKSPIYSHFSETVTGLPIIRAFEHQQRFLAWNEKQIDINQKCVFSWITSNRWLA
                     IRLELVGNLVVFCSALLLVIYRKTLTGDVVGFVLSNALNITQTLNWLVRMTSEAETNI
                     VAVERISEYINVENEAPWVTDKRPPADWPRHGEIQFNNYQVRYRPELDLVLKGITCNI
                     KSGEKVGVVGRTGAGKSSLTNCLFRILESAGGQIIIDGIDVASIGLHDLRERLTIIPQ
                     DPILFSGSLRMNLDPFNKYSDEEVWRALELAHLRSFVSGLQLGLLSEVTEGGDNLSIG
                     QRQLLCLGRAVLRKSKILVLDEATAAVDLETDSLIQTTIRKEFSQCTVITIAHRLHTI
                     MDSDKIMVLDNGKIVEYGSPEELLSNRGSFYLMAKEAGIENVNHTEL"
     exon            244..417
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            418..543
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            544..678
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            679..786
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            787..842
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1074
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1232
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1410
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1665
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1731
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1869
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..2016
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2101
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2168
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2169..2295
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2472
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..2640
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2641..2821
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2822..2948
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            2949..3084
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3304
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3305..3459
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3615
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3616..3815
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3816..3942
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            3943..4044
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4045..4188
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4189..4347
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4348..4514
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4515..4709
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
     exon            4710..5052
                     /gene="Abcc2"
                     /gene_synonym="Cmoat; cMRP; mrp; Mrp2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atgtctgctc actgggatga catagcgtac aacattcaga gaagttaact attaagtcgt
       61 caggatgaaa ggtcaggagg caggccttta actgggctgt gagaatggag aaagcacggt
      121 gcactttaac atctgctttc ccagaggaaa aagtaaagaa gaaacagtac aatcatagaa
      181 gagtcttcgt aacagaagcg cgaggagagc attatggaca agttctgcaa ctctactttt
      241 tgggatctct cattactgga aagtccagag gctgacctgc ctctttgttt tgagcaaact
      301 gttctggtgt ggattccctt gggctttctt tggctcctgg ctccttggca actttacagc
      361 gtgtacagat ccaggaccaa gagatcttct ataaccaaat tctaccttgc caagcaggtg
      421 ttcgtcgtgt ttcttcttat tttagcagcc atagacctgt ctcttgcgct cacagaagat
      481 actggacaag ccacagttcc tcctgtcaga tatacgaatc caatcctcta cctgtgcaca
      541 tggctcctgg ttttggcagt ccagcacagc aggcaatggt gtgtacgaaa gaactcttgg
      601 ttcctgtctc tgttctggat cctctcggtc ttatgcggcg tattccagtt tcagactctg
      661 atacgagcac tcctgaagga cagcaagtcc aacatggcct actcctacct gttcttcgtc
      721 tcctacggtt tccagattgt cctcctgatt cttacagcct tttcaggacc aagtgactca
      781 acacaaactc catcagtcac ggcttccttt ctgagtagca ttacatttag ttggtatgac
      841 aggactgttc tgaaaggtta caagcatcca ctgacactag aagatgtctg ggatatcgat
      901 gaagggttta aaacaaggtc agtcaccagc aagtttgagg cggccatgac aaaggacctg
      961 cagaaagcca ggcaggcttt tcagaggcgg ctgcagaagt cccagcggaa acctgaggcc
     1021 acactacacg gactgaacaa gaagcagagt cagagccaag acgttctcgt cctggaagaa
     1081 gcgaaaaaga agtctgagaa gaccaccaaa gactatccca aatcgtggtt gatcaagtct
     1141 ctcttcaaaa ccttccacgt agtgatcctg aaatcattta tactgaaatt aatacatgac
     1201 cttttggtgt ttctgaatcc tcagctgctg aagttgctga tcggtttcgt gaagagctct
     1261 aactcatacg tgtggtttgg ctatatctgt gcaatcctaa tgtttgctgt gactctcatc
     1321 caatctttct gccttcagtc ttactttcaa cattgttttg tgttgggaat gtgcgtacgg
     1381 acaaccgtca tgtcttcgat atataagaag gcattgaccc tatctaactt ggctaggaag
     1441 cagtacacca ttggagagac ggtgaacttg atgtctgtag attcccagaa gctaatggat
     1501 gcgaccaact acatgcagtt ggtgtggtca agtgttatac agattacttt gtccatcttc
     1561 ttcctgtgga gagagttggg accgtccatc ttagcaggtg ttggggttat ggttctccta
     1621 atcccagtta atggagttct ggctaccaag atcagaaata ttcaggtcca aaatatgaag
     1681 aataaagaca aacgtttaaa aatcatgaat gagattctca gtggaatcaa gatcctgaaa
     1741 tactttgcct gggaaccttc atttcaagag caagtccagg gcattcggaa gaaagaactc
     1801 aagaacttgc tgcggttcgg ccagctgcag agtctgctga tcttcatttt acagataact
     1861 ccaatcctgg tgtctgtggt cacattttct gtctatgtcc tggtggatag cgccaatgtt
     1921 ttgaatgcgg agaaggcatt tacctccatc accctcttca atatcctacg cttccctctg
     1981 tccatgcttc ccatggtgac ctcatcgatc ctccaggcca gtgtttctgt ggaccggctg
     2041 gagaggtatt tgggaggaga cgatttagac acatctgcca ttcgccgcgt cagcaatttt
     2101 gataaagctg tgaagttttc agaggcctct tttacttggg acccggactt ggaagccaca
     2161 atccaagatg tgaacctgga cataaagcca ggccaactgg tggctgtggt gggcactgta
     2221 ggctctggga aatcctcttt ggtatcagcc atgctgggag aaatggaaaa cgttcacggg
     2281 cacatcacca tccagggatc cacagcctat gtccctcagc agtcctggat tcagaatgga
     2341 accatcaaag acaacatcct gtttgggtcc gaatacaatg aaaagaagta ccagcaagtt
     2401 ctcaaagcat gcgctctcct cccagacttg gaaatattgc ctggaggaga catggctgag
     2461 atcggagaga aggggataaa tctcagtggt ggtcagaagc agcgagtcag cctggccaga
     2521 gctgcctatc aagatgctga catctatatt ctggacgatc ccctgtcggc tgtggatgct
     2581 catgtgggaa aacacatttt caacaaggtt gtgggcccca acggcctgtt ggctggcaag
     2641 acgagaatct ttgttactca tggtattcac ttccttcccc aagtggatga gattgtagtt
     2701 ctggggaaag gcaccatctt agagaaagga tcctatcgtg acctgttgga caagaaggga
     2761 gtgtttgcta ggaactggaa gaccttcatg aagcattcag ggcctgaagg agaggccaca
     2821 gtcaataatg acagtgaggc ggaagacgac gatgatgggc tgattcccac catggaggaa
     2881 atccctgagg atgcagcttc cttggccatg agaagagaaa atagtcttcg ccgtacactg
     2941 agccgcagct ctaggtccag cagccgacgt gggaagtccc tcaaaaactc cttgaagatt
     3001 aaaaatgtga atgtcttgaa ggagaaggaa aaagaagtgg aaggacaaaa actaattaag
     3061 aaagaatttg tggaaaccgg gaaggtcaag ttctccatct acctgaagta tctacaggca
     3121 gtagggtggt ggtccatact tttcatcatc cttttctacg gattgaataa tgttgctttt
     3181 atcggctcta acctctggct gagtgcttgg accagtgact ctgacaactt gaatgggacc
     3241 aacaattcgt cttctcatag ggacatgaga attggggtct ttggagctct gggattagca
     3301 caaggtatat gtttgcttat ttcaactctg tggagcatat atgcttgcag aaatgcatca
     3361 aaagctttgc acgggcagct gttaaccaac atcctccggg cacccatgag gttttttgac
     3421 acaactccca caggccggat tgtgaacaga ttttctggtg atatttctac tgtggacgac
     3481 ttgctccccc agacacttcg aagctggatg atgtgtttct ttggcatcgc tggcactctt
     3541 gtcatgatct gcatggccac cccagtcttc gctatcatca tcattcctct cagcattctt
     3601 tatatttcgg tgcaggtttt ttatgtggct acttcccgcc agctgagacg gttggattct
     3661 gtcaccaaat ctccgatcta ttctcacttc agtgagactg tcacaggttt gcccattatc
     3721 cgtgcctttg agcaccagca gcgatttcta gcttggaatg agaagcagat tgacatcaac
     3781 cagaaatgtg tcttttcctg gattacctcc aacaggtggc ttgcaattcg gctggagctg
     3841 gttggaaact tggtcgtctt ctgttccgcc ttgctgctgg ttatttatag aaaaacctta
     3901 accggggacg ttgtgggctt tgttctgtcc aacgccctca atatcacaca aaccttgaac
     3961 tggctagtga ggatgacgtc agaagcagag accaacattg tggcagttga gcgaataagt
     4021 gaatacataa atgtagagaa tgaggcgccc tgggtgactg acaagaggcc tccggcagac
     4081 tggcccagac atggtgagat ccagtttaac aactatcaag tgcggtatcg gccggagctg
     4141 gatctggtac tgaaagggat cacttgtaac atcaagagcg gagagaaggt cggcgtagtg
     4201 ggcaggactg gggctgggaa atcatccctc acaaactgcc tcttcagaat cttagagtct
     4261 gcggggggcc agatcatcat tgatgggata gatgttgcct ccattggact gcacgacctt
     4321 cgagagaggc tgaccatcat tccccaggac cccattttgt tctcggggag tctgaggatg
     4381 aatctcgacc ctttcaacaa atattcagat gaggaggttt ggagggccct ggagttggct
     4441 cacctcagat cctttgtgtc tggcctacag cttgggttgt tatccgaagt gacagagggt
     4501 ggtgacaacc tgagcatagg gcagaggcag ctcctatgcc tgggcagggc tgtgcttcga
     4561 aaatccaaaa tcctggtcct ggatgaagcc acggctgcag tggatctcga gacggatagc
     4621 ctcattcaga cgaccatccg aaaggagttc tcccagtgca cggtcatcac catcgctcac
     4681 aggctgcaca ccatcatgga cagtgacaag ataatggtcc tagacaacgg gaagattgtc
     4741 gagtatggca gtcctgaaga actgctgtcc aacagaggtt ccttctatct gatggccaag
     4801 gaagccggca ttgaaaatgt gaatcacaca gagctctagc agctggttcc gtggctggcg
     4861 gactataaga acagtttcta ttatttgctt tggtttctgt gactgtgctc taggtgcaaa
     4921 gacacatatt ttgttcccgt tgctcaggct ggcctcaaac tctaaggctc cagcaatctc
     4981 tggtctcagc cagagacctg taaaaataga cacttcaaag attatcatga ataaatattt
     5041 aaataaatag taaaaaaaaa aaaaaaaaaa aaa
//
